The glaciers in southeastern Tibet Plateau (SETP) influenced by oceanic climate are sensitive to global warming, and there remains a notable deficiency in accurate multitemporal change analyses of these glaciers. We conduct glacier inventories in the Yigong Zangbo River Basin (YZRB) in SETP for the years 1988, 2015, and 2023 utilizing Landsat and Sentinel-2 imagery, and analyze the glacier spatiotemporal variation incorporating the existing glacier inventory data. Since the 1970s until 2023, the glaciers significantly retreated at a rate of 0.76 ± 0.11%·a−1, with the area decreasing from 2583.09 ± 88.80 km2 to 1635.89 ± 71.74 km2, and the ice volume reducing from 221.7017 ± 7.9618 km3 to 152.7429 ± 6.1747 km3. The most significant retreat occurred in glaciers smaller than 1 km2. Additionally, glaciers on southern aspects retreated slower than the northern counterparts. The glaciers in the western YZRB witnessed a significantly greater shrinkage rate than those in the eastern section, with the most pronounced changes occurring in Aso Longbu River Basin. Furthermore, severe glacier mass deficits were observed from 2000 to 2019, averaging a loss rate of 0.57 ± 0.06 m w.e. a−1. The continuous rise in air temperature has primarily induced a general widespread glacier change in the YZRB. However, diverse topography led to spatial variability in glacier changes with discrepancies as large as several times. The features of individual glaciers, such as glacier size, debris cover, and the development of ice-contact glacial lakes enhanced the local complexity of glacier change and elusive response behaviors to climate warming led by the different topographic conditions.