Gingivitis In Dogs Research Articles

Therapeutic effect of aged garlic extract on gingivitis in dogs.

Periodontal disease is one of the most common dental health problems in dogs. Clinical studies in humans have shown that aged garlic extract (AGE), which contains stable and water-soluble sulfur-containing bioactive compounds, improves the symptoms of periodontal diseases. Our previous study demonstrated that oral administration of AGE in healthy Beagle dogs at 90 mg/kg/day for 12 weeks had no adverse effects such as hemolytic anemia, which is well known to occur as a result of ingestion of Allium species, including onions and garlic, in dogs. However, the therapeutic potential of AGE in canine periodontal disease remains unclear. Accordingly, we investigated the therapeutic effects of AGE in Beagle dogs with mild gingivitis. Feeding 18 mg/kg/day of AGE for 8 weeks resulted in the improvement of gingival index score, level of volatile sulfur compounds in exhaled air, and enzyme activity of periodontal pathogens without any adverse effects on clinical signs and hematological and serum biochemical parameters. Moreover, AGE increased the concentration of salivary cathelicidin, an antimicrobial peptide that contributes to the oral innate immune response. These results suggest that AGE could be a potential therapeutic agent for canine gingivitis.

Effectiveness of silver diamine fluoride 38% on reduction of gingivitis in dogs: a randomized clinical trial.

Periodontal disease is a ubiquitous disease in small animal veterinary medicine. Currently regular professional dental cleaning and daily tooth brushing are considered gold standards in the prevention of periodontal disease. Efforts to find a noninvasive, cost effective and easy to use preventative for periodontal disease are ongoing. The primary objective of this double-blind randomized clinical trial was to determine if a single application of silver diamine fluoride (SDF) 38% on the buccal surface of all teeth would reduce gingivitis within 3 months in dogs with stage two periodontal disease. Twenty-nine client-owned dogs 3-12 years old, 6-35 pounds were randomized 1:1 into active and placebo-control groups. Both groups underwent a baseline treatment and a three-month follow-up under general anesthesia. Gingival Index (GI), Plaque Index (PI), and Calculus Index (CI) were assessed and recorded at each event. A single application of SDF 38% did not significantly lower GI relative to the control group. However, the GI score dropped significantly in both groups relative to baseline, with a 53% reduction in the average GI score for dogs that received SDF 38% treatment and a 44% reduction for dogs that received placebo treatment. There were no differences in PI or CI scores compared to control groups. Further research is needed to determine if a more frequent application or a longer study duration would yield a different outcome.

Features of the course and treatment of chronic catarrhal gingivitis in dogs

Periodontal disease is known to be the most common and serious health problem in dogs today. Despite the rather large arsenal of medicinal substances, the problem of treatment and prevention of periodontal disease remains relevant. Based on this, the purpose of the work was to study the features of chronic catarrhal gingivitis and to develop an effective treatment regimen for dentistically ill dogs. For research, two groups of animals with chronic catarrhal gingivitis were formed in 5 dogs in each (control and experimental), selected on the principle of analogues in terms of age, character and localization of the inflammatory process. Animals of the control and experimental groups were performed tartar removal, irrigation of the oral cavity with water and drying of the mucosa with a sterile gauze swab. Dogs of the control group on the mucous membrane was applied 1 ml of septogel 2 times a day, and the experimental – argumentistin 2 times a day. Before and after the procedures on the mucous membrane was applied a 3 % solution of hydrogen peroxide. It was determined the prevalence of dental disease in dogs, the influence of microflora on the condition of the oral cavity of dogs with chronic catarrhal gingivitis, the clinical condition and the main indices and samples were studied, reflecting the intensity of the inflammatory process in the gums of sick dogs and was developed a method of treatment. The results of researches have shown that the use of argumentistin in the complex treatment of dogs with chronic catarrhal gingivitis helps to reduce the recovery period by 5 days.

Long-term follow-up study after administration of a canine interferon-α preparation for feline gingivitis.

Intraoral administration of canine interferon alpha (CaIFN-α) has been shown to reduce gingivitis in dogs, but has not been confirmed in cats. Therefore, in this study, a CaIFN-α preparation was used for feline gingivitis, and the degree and duration of its effects were examined. Cats were divided into two groups: one was administered CaIFN-α, and the other was not. They were observed up to 12 months. It was suggested that CaIFN-α have a reducing effect on gingivitis and halitosis for a certain period although may not on plaque or calculus. In addition, the duration of the CaIFN-α gingivitis-reducing effect was suggested to be about three months. The CaIFN-α preparation is considered to be a useful treatment for oral hygiene control.

Features of the course and treatment of chronic catarrhal gingivitis in dogs

Chronic catarrhal gingivitis is a polyetiological disease, in the pathogenesis of which the main role belongs to the microorganisms of the oral cavity of the dog, odontogenic deposition in the form of dental plaques and tartar. These depositions, due to the constant irritation of the gums cause disease, the main clinical signs of which are halitosis, poor feed intake, swelling and bleeding gums. The problem of chronic catarrhal gingivitis treatment is devoted to numerous inverstigations, the priority of which is a comprehensive approach, which includes a step-by-step influence on the etiological and pathogenetic mechanisms of the disease. Therefore, the purpose of the work is to study the features of the course of chronic catarrhal gingivitis and to develop a method for the treatment of dogs with dental disease. The prevalence gingivitis in dogs, the influence of microflora on the condition of the oral cavity in chronic catarrhal gingivitis, was investigated the symptomatology of the disease, was established the value of clinical indicators and dental indices and tests, which reflect the intensity and nature of the inflammatory process in the gums of dogs suffering from chronic catarrhal gingivitis, and a method of treating dental animals was developed. The results of the inverstigations showed that the application by application to the mucous membrane of gums 0.05% solution of chlorhexidine and a gel composition of 4% chitosan ascorbate with metronidazole, and also feeding once a day for 30 days of the vitamin-mineral supplement “Canvit Biocal Plus” in the complex treatment of dogs with chronic catarrhal gingivitis helps to reduce the recovery period of dogs by 5 days.

Frequency of the beginning and features of the course of chronic catarrhal gingivitis in dogs

Out of all diseases of organs and systems of teeth disease in dogs is a group of diseases, the symptoms of which are most often observed only when the process involves not only teeth, but also the tissues surrounding them. Paronodopathies deserve special attention, among which chronic catarrhal gingivitis is a polietiological disease in which pathogenesis has a major role in the microorganisms of the oral cavity of dogs, odontogenic deposits in the form of plaque and dental stone. The disease can be complicated by loss of teeth, deformation, reduced functionality of the tooth-jaw system, the formation of chronic diseases in the body, sensitization and development of various forms of somavtic pathology. It has been established that among surgical diseases of dogs gingivitis is most often detected, which is 31.7%. Among the various forms of gingivitis, the largest percentage is the chronic catarrhal gingivitis, which is registered in 47.5% of dogs, mostly dwarf breed. Somewhat less, namely 20.3%, is acute catarrhal gingivitis, 11.9% is chronic hypertrophic and 8.5% is chronic ulcerative gingivitis. The disease is appeared by galitis, disturbance in the intake of food, edema, bleeding of the gums, lesions of the clear papillae, dental plaque with anthrax, formation of pseudocysts. For the objectivity of disease information, clinical manifestations are presented in the form of numerical expressions and dental indexes. It has been established that chronic catarrhal gingivitis in dogs is characterized by an increase in the number of microorganisms in the biotope of the oral cavity, which contribute to the development of inflammation in the gums.

犬の歯肉炎に対するイヌインターフェロンα製剤投与後の長期経過観察

犬の歯肉炎に対するイヌインターフェロンα製剤投与後の長期経過観察

Microcirculation alterations in experimentally induced gingivitis in dogs.

The present study aimed to morphologically examine the gingival microvascular network using a microvascular resin cast (MRC) technique, and to investigate how inflammatory disease functionally affects gingival microcirculation using laser Doppler flowmetry (LDF). We used four beagle dogs with healthy periodontal tissue as experimental animals. To cause periodontal inflammation, dental floss was placed around the cervical neck portions of the right premolars. The unmanipulated left premolars served as controls, and received plaque control every 7days. After 90days, gingivitis was induced in the experimental side, while the control side maintained healthy gingiva. To perform morphological examinations, we used an MRC method involving the injection of low-viscosity synthetic resin into the blood vessels, leading to peripheral soft-tissue dissolution and permitting observation of the bone, teeth, and vascular cast. Gingival blood flow was estimated using an LDF meter. The control gingival vasculature showed hairpin-loop-like networks along the tooth surface. The blood vessels had diameters of 20-40μm and were regularly arranged around the cervical portion. On the other hand, the vasculature in the experimental group was twisted and gathered into spiral forms, with blood vessels that had uneven surfaces and smaller diameters of 8-10μm. LDF revealed reduced gingival blood flow in the group with experimentally induced gingivitis compared to controls. The actual measurements of gingival blood flow by LDF were in agreement with the alterations that would be expected based on the gingivitis-induced morphological alterations observed with the MRC technique.

Effect of Frequency of Brushing Teeth on Plaque and Calculus Accumulation, and Gingivitis in Dogs.

The efficacy of brushing the teeth of beagle dogs in a randomized, controlled, blinded study design using a clearly-defined brushing technique was evaluated for 4 brushing frequencies: brushing daily, brushing every other day, brushing weekly and brushing every other week, compared with no brushing in a control group of dogs. All dogs were fed a standard dry kibble diet during the study. Standard plaque, calculus, and gingivitis indices were used to score the teeth. A 'clean tooth' model was used. No gingival or non-gingival lacerations or other signs of injury to oral tissues were found at the end of the 28 day trial period. Brushing more frequently had greater effectiveness in retarding accumulation of plaque and calculus, and reducing the severity of pre-existing gingivitis. Brushing daily or every other day produced statistically significant improved results compared with brushing weekly or every other week. Based on the results of this study, daily brushing is recommended.

Effect of a Mucoadhesive Gel and Dental Scaling on Gingivitis in Dogs

Twenty client-owned dogs diagnosed with gingivitis were studied over a 45-day period in order to investigate the effect of professional dental prophylaxis combined with the use of a topical mucoadhesive gel containing adelmidrol, an aliamide. A non-intrusive papillary-marginal-gingival index (PMGI) was measured at each assessment, while the gingivitis index (GI) was measured only at the beginning and end of the study. Compared to the control group, the treated dogs had a significant decrease (P < 0.005) in the average GI index during the course of the study. A significant reduction (P < 0.002) in the average PMGI index was observed in both groups 15-days following dental prophylaxis. However at 30 and 45-days following dental prophylaxis, the PMGI index values were significantly different (P < 0.005) from baseline only in treated dogs. These results suggest that the combined use of a mucoadhesive gel with dental scaling was able to improve the regression of gingival inflammation and lengthen the therapeutic benefits of dental scaling and polishing during a limited study period.

Effects of cyclosporine A on plasma cells in experimental gingivitis in dogs

The aim of the present investigation was to evaluate the effect of Cyclosporine A (CsA) on the inflammatory lesion formed in the gingival tissues during de novo plaque formation. 5 beagle dogs were used. On day 0, all teeth of the 5 dogs were scaled and polished. A 6-week period of plaque control including daily tooth cleaning with toothbrush and dentifrice was initiated. A clinical examination regarding plaque and gingivitis was performed, and the plaque control measures were abandoned on the right side of mandible. 3 weeks later, the clinical examination was repeated, samples of subgingival plaque harvested and biopsies obtained from the 3rd and 4th right mandibular premolar regions. The tooth cleaning measures on the left side of the mandible were terminated at this interval. During the following 3 weeks, the animals formed plaque in the lower left premolar regions, and received, 1 x daily, a subcutaneous injection of CsA. At the end of this 2nd plaque formation period (test), the clinical examination was repeated, subgingival plaque was sampled and biopsies from the 3rd and 4th left mandibular premolar regions harvested. The biopsies were prepared for histometric and morphometric analyses. The clinical and histological examinations demonstrated that plaque formation resulted in a gingival lesion (ICT) which, in the 2 periods, had similar size and apical extension. The ICT formed during the CsA administration period, however, harbored an increased number of plasma cells and a reduced macrophage density than the control lesion. It is suggested that CsA administration may result in a Th-2 (T-helper 2-cell) dependent activation of B-lymphocytes.

Clinical observations on the use of oral amoxycillin/clavulanate in the treatment of gingivitis in dogs and cats and anal sacculitis in dogs

Clinical observations on the use of oral amoxycillin/clavulanate in the treatment of gingivitis in dogs and cats and anal sacculitis in dogs

Laser Doppler measurement of gingival blood flow in dogs with increasing and decreasing inflammation

Dramatic changes in vessel morphology and vascularization are associated with early clinical signs of gingivitis in the dog; there is conflicting evidence of both increased and decreased blood flow in the inflamed gingiva. The blood-flow rates were compared in treated and untreated gingivitis in dogs by a new, non-invasive technique, the laser Doppler flow meter (LDF). The LDF measures blood flow in 1 mm 3 of the gingival margin by back-scattered light. Four adult mongrel dogs with generalized mild gingivitis were fed a dental-plaque-inducing diet. Teeth on the left were scaled and polished at zero and four weeks and brushed six times a week during the seven-week experimental period; teeth on the right were left untreated. Test teeth were the upper canines through to the fourth premolars, and lower second premolars through to the first molars. On the facial aspect of these teeth, plaque index (PlI), gingival index (GI), and gingival crevicular fluid (GCF) were assessed at weeks zero, one, four and seven. Blood flow at the gingival margin was measured with LDF at weeks one, four and seven. Acrylic appliances were used to reposition the fibre-optic probe in the same place at each follow-up examination. In a conditional binary logistic regression model, PlI, GI and GCF were significantly related to treatment ( p < 0.01), but LDF blood flow was not ( p = 0.01). Blood flow increased slightly with time on the untreated side, but there was no significant decrease in blood flow with the resolution of inflammation on the treated side. Thus microvascular changes in gingival inflammation may not quickly, if ever, be reversed.

Lymphoproliferative response of submandibular nodes to mitogens in experimental gingivitis in dogs

The purpose of the present study was to evaluate the lymphoproliferative response of submandibular nodes cells to stimulation by phytohemagglutinin (PHA), concanavalin A (Con A), and lipopolysaccharide (LPS) following experimental gingivitis in dogs.In 4 dogs of the experimental group, gingival inflammation was induced by cervical ligatures on the left jaw, and the right jaw was kept clean by toothbrushing. In 2 dogs of the control group, toothbrushing was performed throughout. After the weight and lymphocyte number in the submandibular nodes were calculated, the lymphocyte response was determined by the uptake of 3H‐thymidine.The weight and lymphocyte number of the ipsilateral nodes increased 2.2 and 3.7 times, respectively, as compared with the contralateral nodes. There was a higher background proliferation of unstimulated lymphocytes from ipsilateral nodes. The lymphocyte response to lower PHA concentration from ligated side lymphnodes significantly increased compared to that from contralateral nodes. It is suggested that the plaque accumulation and the intensive gingival inflammation considerably influence lymphocytes in the submandibular nodes.

Effect of selective antimicrobial therapy on plaque and gingivitis in the dog

The present investigation was performed to assess the effect of selective antibiotic therapy on developing plaque and gingivitis in dogs, which at the start of the study had normal gingiva. Fifteen beagle dogs were used. Throughout the entire observation period the animals were fed a diet which favored plaque accumulation. A baseline examination involved assessments of plaque, gingivitis and gingival exudate. The subgingival bacterial flora was assessed by dark-field microscopy. Subsequently the teeth of the right jaws were allowed to accumulate plaque. A careful tooth-cleaning program was maintained in the left jaws. Plaque and gingivitis assessments were repeated and subgingival plaque sampled in the right jaws after 14 and 28 days. On experimental day 28 the second part of the study was initiated. The dogs were randomly distributed into three groups of five animals each. A new baseline examination was performed in the left jaws, after which all tooth cleanings were terminated. During the subsequent 28 days each group of dogs was treated with one of three antimicrobial compounds (vancomycin, metronidazole or clindamycin). Examinations were repeated after 14 and 28 days. The results demonstrated that systemic administration of antimicrobial substances can reduce the rate of plaque formation, change the composition of the developing subgingival microbiota and prevent (or retard) the onset of gingivitis. A comparison of the ability of the three compounds to prevent the formation of a "gingivitis-inducing" plaque revealed that metronidazole and clindamycin were markedly more effective than vancomycin. In fact, in dogs receiving metronidazole and clindamycin treatment, the initiation of gingivitis was almost entirely prevented during the 28 days of treatment.

The appearance of bone resorbing activity in ligature induced gingivitis in dogs.

The in vitro bone resorbing activity of extracts from healthy and inflamed dog gingiva were compared and the appearance of the resorbing factor between 1 and 6 weeks of gingivitis was measured. Seven adult dogs were used. The gingiva were initially rendered healthy by scaling and polishing. Plaque retentive ligatures were placed around the necks of teeth so that at the end of the experiment samples of healthy gingiva and gingiva with 1 and 6 week old ligature induced gingivitis could be harvested from each dog. The bone resorbing activity of extracts from these gingival samples was studied by measuring the release of 45Ca from prelabeled fetal rat long bones in organ culture. Six out of 7 extracts from healthy quadrants had no bone resorbing potential whereas 13 out of 14 extracts from ligatured quadrants stimulated significant amounts of bone resorption. The resorbing activity appeared during the first week after ligature placement and no significant increase in resorbing potential was noted between extracts obtained 1 and 6 weeks after ligature placement. It was concluded that the appearance of the bone resorbing factor(s) is related to the accumulation of plaque and/or the resultant inflammation and that it may be responsible for the alveolar bone loss seen in ligature induced periodontal disease in dogs.

Immunofluorescent study on experimentally induced gingivitis in dogs (author's transl)

In the present study the localizations of immunoglobulins (Ig) and lymphocytes (Ly) were investigated in the initial, early and established lesions of gingivitis experimentally induced in dogs by fluorescent antibody techniques.After the bimaxillary premolars were brushed twice a day for three months to obtain a clinically healthy gingiva, the experimental gingivitis was induced by accumulation of plaque following ligature with floss silk. Antisera against dog IgG, IgA, IgM, T cell and B cell raised in rabbits were conjugated with FITC. Histological and immunofluorescent investigations were made on the gingival biopsy specimens obtained at O day, one week, one month and three months after the start of plaque accumulation.The following findings were obtained:In a clinically healthy gingiva, faint staining for Ig was observed in the connective tissue (CT) adjacent to the sulcular epithelium (SE). At one week, Ig was obviously observed in the SE and perivascular areas in the CT. A number of polymorphonuclear leukocytes and a few T cells were noticed in the CT adjacent to the SE. With progress of the inflammation, T cells were infiltrated in the marginal and sulcular regions, where Ig was stained in fine granules. At three months, B cells and plasma cells were detected mainly in the CT.

Resolution of Gingivitis in Dogs Following Topical Applications of 0.4% Stannous Fluoride and Toothbrushing

Resolution of Gingivitis in Dogs Following Topical Applications of 0.4% Stannous Fluoride and Toothbrushing

Effect of experimental neutropenia on initial gingivitis in dogs

The role of neutrophilic granulocytes in the loss of gingival collagen has been studied by inducing experimental neutropenia during initial gingivitis in beagle dogs. Neutropenia was induced for 4 d in three animals with normal gingiva by repeated injections of rabbit anti-neutrophil serum. During neutropenia microbial plaque was allowed to form on the teeth. Samples of junctional (crevicular) leukocytes and gingival fluid were taken on days 0 and 4. Block biopsies of buccal gingiva were obtained on day 4. Stained semi- and ultrathin sections were used for histometric and stereologic tissue analysis. Gingival fluid flow increased from day 0 to day 4 in all dogs while junctional leukocytes increased in one dog only. Subgingival plaque had formed in most biopsies, and in the junctional epithelium very few neutrophilic granulocytes were present. In the coronal connective tissue subjacent to the junctional epithelium lymphoid cells, structurally abnormal neutrophilic granulocytes and monocytes/macrophages were diffusely scattered. The gingival collagen appeared mainly displaced by the inflammatory cells rather than dissolved. The data suggest that neutrophilic granulocytes may contribute to the loss of gingival collagen during initial gingivitis in dogs. The neutrophils also seem to be of importance for the limitation of subgingival plaque growth along the tooth surface.

The effect of systemic antimicrobial therapy on plaque and gingivitis in dogs

Six dogs with varying degrees of gingivitis wer divided into 3 groups of 2 dogs each. One pair of dogs received tetracycline HCI 250 mg, 2 times/day, for 4 weeks. The controls reveived no medication during the experimental period of 4 weeks. The results showed a decrease in the proportions of coccoid cells and straight rods in the periodontal flora with increasing gingival inflammation, while the proportions of spirochetes and motile bacteria were markedly reduced. Metronidazole appeared to be somewhat more effective in eliminating spirochetes than tetracycline. Treatment with the antimicropbial drugs tended to derease the size of th infiltrated connective tissue as well as the proportion of junctional epithelium occupied by inflammatory cells, but did not succeed in eliminating the inflammatory reaction entirely. At the initial examination around 60% of the leukocyte volume in junctional epthelium consisted of neutrophilic granulocytes. At the final examination less than 30 % of the leukocyte volume consisted of neutrophis. It was not clear from the results if the improvement in the clinical and histological parameters was due to the specific suppression of ertain organisms or to an overall. non‐specific reduction in volume of the periodontal flora.