Immunofluorescent study on experimentally induced gingivitis in dogs (author's transl)

Abstract

In the present study the localizations of immunoglobulins (Ig) and lymphocytes (Ly) were investigated in the initial, early and established lesions of gingivitis experimentally induced in dogs by fluorescent antibody techniques.After the bimaxillary premolars were brushed twice a day for three months to obtain a clinically healthy gingiva, the experimental gingivitis was induced by accumulation of plaque following ligature with floss silk. Antisera against dog IgG, IgA, IgM, T cell and B cell raised in rabbits were conjugated with FITC. Histological and immunofluorescent investigations were made on the gingival biopsy specimens obtained at O day, one week, one month and three months after the start of plaque accumulation.The following findings were obtained:In a clinically healthy gingiva, faint staining for Ig was observed in the connective tissue (CT) adjacent to the sulcular epithelium (SE). At one week, Ig was obviously observed in the SE and perivascular areas in the CT. A number of polymorphonuclear leukocytes and a few T cells were noticed in the CT adjacent to the SE. With progress of the inflammation, T cells were infiltrated in the marginal and sulcular regions, where Ig was stained in fine granules. At three months, B cells and plasma cells were detected mainly in the CT.