Gibberellic Acid Research Articles

Developing an Optimized Protocol for Regeneration and Transformation in Pepper.

Capsicum annuum L. is extensively cultivated in subtropical and temperate regions globally, respectively, when grown in a medium with 8 holding significant economic importance. Despite the availability of genome sequences and editing tools, gene editing in peppers is limited by the lack of a stable regeneration and transformation method. This study assessed regeneration and transformation protocols in seven chili pepper varieties, including CM334, Zunla-1, Zhongjiao6 (ZJ6), 0818, 0819, 297, and 348, in order to enhance genetic improvement efforts. Several explants, media compositions, and hormonal combinations were systematically evaluated to optimize the in vitro regeneration process across different chili pepper varieties. The optimal concentrations for shoot formation, shoot elongation, and rooting in regeneration experiments were determined as 5 mg/L of 6-Benzylaminopurine (BAP) with 5 mg/L of silver nitrate (AgNO3), 0.5 mg/L of Gibberellic acid (GA3), and 1 mg/L of Indole-3-butyric acid (IBA), respectively. The highest regeneration rate of 41% was observed from CM334 cotyledon explants. Transformation optimization established 300 mg/L of cefotaxime for bacterial control, with a 72-h co-cultivation period at OD600 = 0.1. This study optimizes the protocols for chili pepper regeneration and transformation, thereby contributing to genetic improvement efforts.

Response of Elymus nutans Griseb. seedling physiology and endogenous hormones to drought and salt stress

Elymus nutans Griseb. (E. nutans), a pioneer plant for the restoration of high quality pasture and vegetation, is widely used to establish artificial grasslands and ecologically restore arid and salinized soils. To investigate the effects of drought stress and salt stress on the physiology and endogenous hormones of E. nutans seedlings, this experiment configured the same environmental water potential (0 (CK), − 0.04, − 0.14, − 0.29, − 0.49, − 0.73, and − 1.02 MPa) of PEG-6000 and NaCl stress to investigate the effects of drought stress and salt stress, respectively, on E. nutans seedlings under the same environmental water potential. The results showed that although the physiological indices and endogenous hormones of the E. nutans seedlings responded differently to drought stress and salt stress under the same environmental water potential, the physiological indices of E. nutans shoots and roots were comprehensively evaluated using the genus function method, and the physiological indices of the E. nutans seedlings under the same environmental water potential exhibited better salt tolerance than drought tolerance. The changes in endogenous hormones of the E. nutans seedlings under drought stress were analyzed to find that treatment with gibberellic acid (GA3), gibberellin A7 (GA7), 6-benzyladenine (6-BA), 6-(y,y-dimethylallylaminopurine) (2.IP), trans-zeatin (TZ), kinetin (KT), dihydrozeatin (DHZ), indole acetic acid (IAA), and 2,6-dichloroisonicotininc acid (INA) was more effective than those under drought stress. By analyzing the amplitude of changes in the endogenous hormones in E. nutans seedlings, the amplitude of changes in the contents of GA3, GA7, 6-BA, 2.IP, TZ, KT, DHZ, IAA, isopentenyl adenosine (IPA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), and abscisic acid was larger in drought stress compared with salt stress, which could be because the endogenous hormones are important for the drought tolerance of E. nutans itself. The amplitude of the changes in the contents of DHZ, TZR, salicylic acid, and jasmonic acid was larger in salt stress compared with drought stress. Changes in the content of melatonin were larger in salt stress compared with drought stress, which could indicate that endogenous hormones and substances are important for the salt tolerance of E. nutans itself.

Dormancy induced by floating starvation method in Nymphaea atrans

In plants, dormancy is induced by numerous environmental stressors. In Hainan's tropical climate, dormancy is crucial for the repropagation of water lily plants, and the tubers emerging from dormancy can produce 3–5 plantlets; however, tropical water lilies often resist entering this stage even in cold and short-day conditions, leading to frost injuries and death. In this study, we employed the floating starvation method (FSM) to induce nutritional deficiency, driving tropical water lily plants into dormancy. We examined the effects of various durations of FSM (0, 13, 26, and 40 days) on Nymphaea atrans plants. Our results indicated that nutritional stress was essential for inducing dormancy in N. atrans, with plants beginning to enter dormancy after approximately 40 days of treatment. During this period, the dry matter content of the tubers significantly increased, unlike in the leaves, suggesting an enhanced allocation of underground resources in response to nutrient stress. The tubers accumulated substantial amounts of carbohydrates, soluble sugars, and starch, providing the energy needed to break dormancy during subsequent growth. Increases in free proline, as well as and the activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and malondialdehyde (MDA), indicated that N. atrans initiated an active oxygen-scavenging system to mitigate oxidative stress. A notable increase in abscisic acid (ABA) content was observed, accompanied by a decrease in gibberellic acid (GA3) and indole-3-acetic acid (IAA) levels. This prolonged nutritional stress significantly increased ABA levels, leading to leaf and stem abscission and a reduction in GA3 and IAA through decomposition, finally resulting in dormancy. These findings provide new insights into the potential regulatory mechanisms of tuber dormancy induction through FSM in N. atrans.

Gibberellic Acid and Benzyl Adenine Improve Garlic Seed Cloves Sprouting and Boost Plant Growth and Productivity in Unfavorable Conditions

Gibberellic Acid and Benzyl Adenine Improve Garlic Seed Cloves Sprouting and Boost Plant Growth and Productivity in Unfavorable Conditions

Effect of zinc and gibberellic acid on growth and yield of cluster bean

Effect of zinc and gibberellic acid on growth and yield of cluster bean

A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus.

Dwarfing is a pivotal agronomic trait affecting both yield and quality. Citrus species exhibit substantial variation in plant height, among which internode length is a core element. However, the molecular mechanism governing internode elongation remains unclear. Here, we unveiled that the transcriptional cascade consisting of B-BOX DOMAIN PROTEIN 22 (BBX22) and ELONGATED HYPOCOTYL 5 (HY5) finely tunes plant height and internode elongation in citrus. Loss-of-function mutations of BBX22 in an early-flowering citrus (Citrus hindsii "SJG") promoted internode elongation and reduced pigment accumulation, whereas ectopic expression of BBX22 in SJG, sweet orange (C. sinensis), pomelo (C. maxima) or heterologous expression of BBX22 in tomato (Solanum lycopersicum) significantly decreased internode length. Furthermore, exogenous application of gibberellin A3 (GA3) rescued the shortened internode and dwarf phenotype caused by BBX22 overexpression. Additional experiments revealed that BBX22 played a dual role in regulation internode elongation and pigmentation in citrus. On the one hand, it directly bound to and activated the expression of HY5, GA metabolism gene (GA2 OXIDASE 8, GA2ox8), carotenoid biosynthesis gene (PHYTOENE SYNTHASE 1, PSY1) and anthocyanin regulatory gene (Ruby1, a MYB DOMAIN PROTEIN). On the other hand, it acted as a cofactor of HY5, enhancing the ability of HY5 to regulate target genes expression. Together, our results reveal the critical role of the transcriptional cascade consisting of BBX22 and HY5 in controlling internode elongation and pigment accumulation in citrus. Unraveling the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.

Physiological Mechanisms Underlying Enhanced Strawberry Shelf-Life through Integrated Gibberellic Acid, Magnetic Field and Temperature

Physiological Mechanisms Underlying Enhanced Strawberry Shelf-Life through Integrated Gibberellic Acid, Magnetic Field and Temperature

Morphological and physiological investigations reveal the regulatory effect of exogenous paclobutrazol on flowering promotion by winter warming in Chaenomeles speciosa ‘Changshouguan’

The application of exogenous paclobutrazol (PP333) can improve the ability of winter warming to promote flowering in Chaenomeles speciosa, but the underlying mechanism is unclear. In this study, the cultivar ‘Changshouguan’ was sprayed with different concentrations of PP333 during flower bud differentiation, and the changes in the anatomical structures and physiological characteristics of the flower buds during the differentiation process, as well as the growth state of the flower buds and the effect on flowering promotion after winter warming treatment, were comprehensively investigated. The results showed that different concentrations of PP333 could advance the flowering time of ‘Changshouguan’ by 15–24 d under the warming treatment and increase the flowering duration to 17 d compared with those under the warming treatment alone (CK), and 1000 mg/L was the best treatment. Compared with the CK treatment, the PP333 treatment decreased the contents of indole acetic acid (IAA) and gibberellic acid (GAs) and increased the contents of zeatin ribosides (ZRs) and abscisic acid (ABA), thus changing the balance of hormones during flower bud differentiation. The inflection point (low point) of the curve shapes of the ZRs/GAs and ZRs/IAA ratios appeared significantly earlier, which showed a pattern consistent with soluble sugar and protein content and antioxidant activity. Interestingly, the above changes also corresponded to earlier flowering times during the warming process. Taken together, these results indicate that spraying an appropriate concentration of PP333 in the early stage of ‘Changshouguan’ flower bud differentiation promotes the early differentiation of flower buds and early flowering under winter warming treatment by altering their endogenous hormone content and homeostasis and changing their physiological state. The key to maintaining a relatively long flowering period in plants in the PP333 treatment group after flowering promotion was the increased accumulation of sugars and proteins.

How to store araticum seeds and maintain their physiological quality

The purpose of seed storage is its preservation; in this sense, the storage period and types of packaging materials utilized in this process interfere with the conservation of seed vigor. The objective here was to evaluate the physiological potential of araticum seeds in different types of packaging materials and storage periods. The work was developed at the State University of Western Paraná Seed Technology Laboratory (Unioeste) - Campus Marechal C. Rondon - from February to August 2020. The seeds were packed in different packaging materials for three months (February, March, and April / 2020), kept in a cold chamber (16º C and 40% RH). The experimental design used was completely randomized, in a 5 x 7 factorial scheme [five packages (glass, plastic bag, burlap bag, kraft paper, and pet bottle) x seven storage periods (0, 15, 30, 45, 60, 75 and 90 days)]. It is important to note that araticum seeds have dormancy, so they were submerged in 500 mg L-1 of gibberellic acid (GA3) for 24 hours before the setting up of the germination test. Evaluating the physiological aspect of the germination and vigor. The kraft paper packaging is the most suitable for storing araticum seeds. The physiological potential of seeds of this species decreases when its storage period is increased.

A delayed response in phytohormone signaling and production contributes to pine susceptibility to Fusarium circinatum

BackgroundFusarium circinatum is the causal agent of pine pitch canker disease, which affects Pinus species worldwide, causing significant economic and ecological losses. In Spain, two Pinus species are most affected by the pathogen; Pinus radiata is highly susceptible, while Pinus pinaster has shown moderate resistance. In F. circinatum-Pinus interactions, phytohormones are known to play a crucial role in plant defense. By comparing species with different degrees of susceptibility, we aimed to elucidate the fundamental mechanisms underlying resistance to the pathogen. For this purpose, we used an integrative approach by combining gene expression and metabolomic phytohormone analyses at 5 and 10 days post inoculation.ResultsGene expression and metabolite phytohormone contents suggested that the moderate resistance of P. pinaster to F. circinatum is determined by the induction of phytohormone signaling and hormone rearrangement beginning at 5 dpi, when symptoms are still not visible. Jasmonic acid was the hormone that showed the greatest increase by 5 dpi, together with the active gibberellic acid 4 and the cytokinin dehydrozeatin; there was also an increase in abscisic acid and salicylic acid by 10 dpi. In contrast, P. radiata hormonal changes were delayed until 10 dpi, when symptoms were already visible; however, this increase was not as high as that in P. pinaster. Indeed, in P. radiata, no differences in jasmonic acid or salicylic acid production were found. Gene expression analysis supported the hormonal data, since the activation of genes related to phytohormone synthesis was observed earlier in P. pinaster than in the susceptible P. radiata.ConclusionsWe determine that the moderate resistance of P. pinaster to F. circinatum is in part a result of early and strong activation of plant phytohormone-based defense responses before symptoms become visible. We suggest that jasmonic acid signaling and production are strongly associated with F. circinatum resistance. In contrast, P. radiata susceptibility was attributed to a delayed response to the fungus at the moment when symptoms were visible. Our results contribute to a better understanding of the phytohormone-based defense mechanism involved in the Pinus-F. circinatum interactions and provide insight into the development of new strategies for disease mitigation.

Effect of Gibberellic Acid and Zinc Sulphate on Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

An experiment was conducted at Horticulture Research Field, Department of Horticulture, Naini Agricultural Institute, Faculty of Agriculture, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, during February-May, 2023 on cucumber. The experiment was laid out in factorial randomized block design with twenty treatment combinations and was replicated thrice. The experiment consisted of two factors. Factor H: Hybrids (TMCU-1107, TMCU-1125, TMCU-3112 and Saira-934) and Factor T: Different concentrations of GA₃+ZnSO₄ (T0 - Control (water spray), T1 -GA₃ @100ppm+ZnSO₄ @0.25%, T2 - GA₃ @200ppm+ ZnSO₄ @0.50%, T3 - GA₃ @300ppm+ ZnSO₄ @0.75% and T4 - GA₃ @400ppm+ZnSO₄ @1%). TMCU-1125 recorded significantly higher yield attributes of cucumber over other hybrids in parameters like vine length at 80 DAS (102.6 cm), days to 50% flowering (40), days to 1st harvest (46), number of fruits per plant (11), fruit length (18.4 cm), average fruit weight (237.5 g), average fruit yield per plant (2.6 kg) and ascorbic acid content (0.8 mg/100g). Among different treatments, GA₃ @300ppm+ ZnSO₄ @0.75% resulted in significantly longer vine at 80 DAS (98.4 cm), Days to 50% flowering (43), days to 1st harvest (50), number of fruits per plant (9), fruit length (16.6 cm), average fruit weight (232.0 g), average fruit yield per plant (2.0 kg) and ascorbic acid content (0.5 mg/100g). The interaction effect of T8 (TMCU-1125 + GA₃ @300ppm+ ZnSO₄ @0.75% is best suited for getting higher growth, yield, quality of cucumber.

Karrikinolide1 (KAR1), a Bioactive Compound from Smoke, Improves the Germination of Morphologically Dormant Apium graveolens L. Seeds by Reducing Indole-3-Acetic Acid (IAA) Levels.

Smoke-water (SW) and Karrikinolide1 (KAR1) release dormancy and improve seed germination in many plant species. Therefore, we tested SW (1:2500 v/v) and KAR1 (10-7 M) to break the morphological dormancy of celery cultivar (Apium graveolens L.). In the first trial, seeds were subjected to a 21-day incubation period at 20 °C with SW and KAR1 applied as single treatments. KAR1 showed significantly improved germination (30.7%) as compared to SW (17.2%) and a water control (14.7%). In seed soaking experiments, SW, KAR1, and gibberellic acid (GA3) treatments showed higher germination percentages than the water control after 3 and 6 h of soaking. However, prolonged soaking (12 h) reduced germination percentages for all treatments, indicating a detrimental effect. Analysis of KAR1 content dynamics in 7-day- and 21-day-old celery seeds indicated its prolonged effects on germination and dormancy alleviation. Phytohormones, including auxins in 7-day-old and cytokinins in 7-day- and 21-day-old celery seedlings, along with their precursors and metabolites, were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) after treatment with KAR1 and SW. The analysis of auxin levels in 7-day-old seeds revealed a negative correlation between seed germination and auxin (indole-3-acetic acid, IAA) content. Notably, it was found that KAR1-treated seeds significantly reduced IAA levels in all treatments. SW and KAR1 did not significantly affect cytokinin levels during celery germination except for N6-Isopentenyladenine. Hence, further research is needed to understand their precise role in celery seed germination. This work will improve our understanding of the role of bioactive compounds from plant-derived smoke and how they regulate hormonal responses and improve germination efficiency in celery.

Advances in Gibberellic Acid Application in Cropping

Advances in Gibberellic Acid Application in Cropping

Aspects of the joint cultivation of <i>Bacillus amyloliquefaciens</i> and <i>Bacillus aryabhattai</i> for the intensification of growth-stimulating substances synthesis

Growth-stimulating properties of Bacillus amyloliquefaciens B-11265 and Bacillus aryabhattai B-13192 strains were studied, and the possibility of their co-cultivation for activity intensification was evaluated. The strains under study were found to be able to synthesize indolyl-3-acetic and gibberellic acids, siderophores: B. amyloliquefaciens B-11265 in the amount of 5.23 µg/ml, 1.05 mg/ml, 46.15%; B. aryabhattai B-13192 – 3.07 µg/ml, 0.84 mg/ml, 35.50%, respectively. The research results showed that the strains were biocompatible and did not inhibit each other's development, allowing them to be included in the same consortium. Joint cultivation of the strains showed that the ratio of microorganisms in the nutrient substrate has a significant effect on the yield of the target substances. At the ratio of B. amyloliquefacien and B. aryabhattai 1 : 1, inhibition of indolyl-3-acetic acid synthesis was observed; ratios of 2 : 3 and 3 : 2 led to a decrease in gibberellic acid production. It was found that three (B. amyloliquefacien) to one (B. aryabhattai) was the optimal ratio to increase the yield of the target substances compared to the average values characteristic of individual strains. In this variant, the synthesis of growth-stimulating substances by the consortium was: indolyl-3-acetic acid – 5.31 μg/ml, gibberellic acid – 1.23 mg/ml, siderophores – 51.27%. The results obtained indicate the high potential of the engineered consortium for agricultural applications. However, in order to develop a growth-stimulating preparation based on it, it is necessary to conduct a number of additional studies aimed at investigating the effect of the strain on agricultural crops in laboratory and field experiments.

GhSBI1, a CUP-SHAPED COTYLEDON 2 homologue, modulates branch internode elongation in cotton.

Branch length is an important plant architecture trait in cotton (Gossypium) breeding. Development of cultivars with short branch has been proposed as a main object to enhance cotton yield potential, because they are suitable for high planting density. Here, we report the molecular cloning and characterization of a semi-dominant quantitative trait locus, Short Branch Internode 1(GhSBI1), which encodes a NAC transcription factor homologous to CUP-SHAPED COTYLEDON 2 (CUC2) and is regulated by microRNA ghr-miR164. We demonstrate that a point mutation found in sbi1 mutants perturbs ghr-miR164-directed regulation of GhSBI1, resulting in an increased expression level of GhSBI1. The sbi1 mutant was sensitive to exogenous gibberellic acid (GA) treatments. Overexpression of GhSBI1 inhibited branch internode elongation and led to the decreased levels of bioactive GAs. In addition, gene knockout analysis showed that GhSBI1 is required for the maintenance of the boundaries of multiple tissues in cotton. Transcriptome analysis revealed that overexpression of GhSBI1 affects the expression of plant hormone signalling-, axillary meristems initiation-, and abiotic stress response-related genes. GhSBI1 interacted with GAIs, the DELLA repressors of GA signalling. GhSBI1 represses expression of GA signalling- and cell elongation-related genes by directly targeting their promoters. Our work thus provides new insights into the molecular mechanisms for branch length and paves the way for the development of elite cultivars with suitable plant architecture in cotton.

Morphological Changes to Fruit Development Induced by GA3 Application in Sweet Cherry (Prunus avium L.).

Cherry (Prunus avium) fruits are important sources of vitamins, minerals, and nutrients in the human diet; however, they contain a large stone, making them inconvenient to eat 'on the move' and process. The exogenous application of gibberellic acid (GA3) can induce parthenocarpy in a variety of fruits during development. Here, we showed that the application of GA3 to sweet cherry unpollinated pistils acted as a trigger for fruit set and permitted the normal formation of fruit up to a period of twenty-eight days, indicating that gibberellins are involved in the activation of the cell cycle in the ovary wall cells, leading to fruit initiation. However, after this period, fruit development ceased and developing fruit began to be excised from the branch by 35 days post treatment. This work also showed that additional signals are required for the continued development of fully mature parthenocarpic fruit in sweet cherry.

GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth.

The phytohormone gibberellic acid (GA) is critical for environmentally sensitive plant development including germination, skotomorphogenesis, and flowering. The Förster resonance energy transfer biosensor GIBBERELLIN PERCEPTION SENSOR1, which permits single-cell GA measurements in vivo, has been used to observe a GA gradient correlated with cell length in dark-grown, but not light-grown, hypocotyls. We sought to understand how light signaling integrates into cellular GA regulation. Here, we show how the E3 ligase CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1) and transcription factor ELONGATED HYPOCOTYL 5 (HY5) play central roles in directing cellular GA distribution in skoto- and photomorphogenic hypocotyls, respectively. We demonstrate that the expression pattern of the GA biosynthetic enzyme gene GA20ox1 is the key determinant of the GA gradient in dark-grown hypocotyls and is a target of COP1 signaling. We engineered a second generation GPS2 biosensor with improved orthogonality and reversibility. GPS2 revealed a previously undetectable cellular pattern of GA depletion during the transition to growth in the light. This GA depletion partly explains the resetting of hypocotyl growth dynamics during photomorphogenesis. Achieving cell-level resolution has revealed how GA distributions link environmental conditions with morphology and morphological plasticity. The GPS2 biosensor is an ideal tool for GA studies in many conditions, organs, and plant species.

Impact of ultra-high dilutions from Iranian endemic and commercial calendula on the germination and growth quality of Oryza sativa L.

PurposeThis article deals with producing ultra-diluted compounds (UHDs) prepared from Iranian calendula's characteristic and endemic species. It compares their chemical, biological and biochemical characteristics with the commercial sample of calendula species (grown in the Alps). In the following, these UHDs have been used to improve the quality of germination and growth and reduce contamination of rice (Oryza sativa) seeds in the laboratory environment. MethodsHigh-performance thin-layer chromatography (HPTLC) is used to isolate the active compounds. On the separated results, antioxidant and antibacterial were identified directly on the plate (Bio-autographic method). Direct on the plate)DESI mass spectrometry was used to identify the active compounds. ResultsThe HPTLC reveals that the chromatogram of native C. percica and C. officinalis extract is the most similar to the commercial compounds. The highest antioxidant activity is related to C. officinalis. The best antibacterial activity of the extracts against Staphylococcus aureus and Escherichia coli belongs to C. officinalis and C. tripterocarpa. Rutin, quercitrin, β-campstrole and di-o-caffeoylquinic acid, which are among the flavonoid and terpenoid categories were identified as active compounds. The prepared UHDs from native calendula are biologically more effective than the commercial ones in increasing seed germination efficiency, improving rooting quality and reducing contamination. ConclusionUsing UHDs increases the production of photosynthetic pigments the root length and the number of lateral roots. Also, the amount of protein, gibberellic acid and abscisic acid in seedlings treated using native UHDs of C. officinalis (native or commercial) is higher than the others.

Promising antifungal behavior of biosynthesized bimetallic silver-copper oxide nanoparticles and Bacillus safensis against some strawberry rots

Promising nanomaterials combined with beneficial microbes contributed to bio-modulations that might increase yields. In order to combat several fungal infections that invade strawberries (Fragaria ananassa), this study assessed the interactions between the synthesized CuO NPs, bimetallic Ag–CuO NPs, and Bacillus safensis (BS-22) under various treatment circumstances. The effectiveness of BS-22, CuO NPs, and bimetallic Ag–CuO NPs against four different pathogens that cause gray mold disease and root rot on strawberry plants was determined. It was possible to successfully extract twenty-four endophytic bacterial isolates from hygienic strawberry plants that were indigenous. BS-22 isolate, which produced the most hydrogen cyanide (HCN), siderophores, gibberellic acid (GA3), and indole-3-acetic acid (IAA), was identified molecularly as Bacillus safensis. The most extensively studied treatments (foliar CuO NPs at 200 ppm + foliar Ag–CuO NPs at 200 ppm + BS-22) decreased the frequency and severity of black root rot disease in strawberry plants. Foliar CuO NPs (50 ppm) + foliar Ag–CuO NPs (50 ppm) showed significant increase of the total suspended solids (TSSs), total antioxidant capacity (TAC) content, and the total polyphenols content compared to the control and other treatments. The same treatment recorded the highest value in all characters of cell wall components except lignin character where BS-22 treatment had the best effect. All tested treatments significantly decreased disease incidence and severity when strawberry fruit stored at 22 °C ± 2 for 25 days on average. According to this study, foliar CuO NPs (200 ppm) + foliar Ag–CuO NPs (200 ppm) + BS-22 (as a soil drench) might be used as a substitute for gray mold and root rot diseases in strawberry development and biocontrol.

Various presowing treatments for enhancing Melia dubia (Cav.) seed germination, seedling development, and vigor index

Melia dubia (Cav.) is one of the most promising agroforestry multipurpose tree species. However, there is a shortage of this quality planting material due to its low germination ability. Our study assessed six different presowing treatments, namely, soaking in tap water for 24 h, soaking in a cow dung slurry for 7 days (CDS), scarification with 50 % H2SO4 for 5 min, soaking in 200 ppm gibberellic acid (GA3) for 24 h, and soaking in CDS + H2SO4, GA3 + H2SO4 or the control (no application), for enhancing seed germination and growth in Melia dubia during 2019–20 in the Indo-Gangetic Plains region. STAR version 2.0.1 was used to analyze the observed data using one-way ANOVA. The results of this study showed that different treatments substantially impacted the germination and growth of Melia dubia. Compared with those of untreated seeds (control), the germination percentage of seeds treated with cow dung slurry significantly increased by 49 %, the biomass percentage significantly increased by 25 %, and the vigor index significantly increased by 79 %. In conclusion, our study revealed that treating Melia dubia seeds with cow dung slurry for a period of 7 days enhances germination and seedling growth and is also cost-effective for nursery managers and farmers due to the absence of associated costs.