GHz Microwave Radiation Research Articles

The influence of thermal radiation during microwave-assisted freeze-drying of pharmaceutical unit doses

New drying technologies for biologicals have recently been developed to accelerate the time-consuming batch freeze-drying (BFD) process. Among others, microwave-assisted freeze-drying (MFD) has been suggested as a faster and more effective drying technology. In this study, MFD cycles with the microwave radiation switched on and off were performed to assess the contribution of the microwave radiation to drying acceleration. It was found that thermal radiation emitted by the drying chamber walls was predominantly accelerating the drying of monodose placebos rather than microwave radiation. The combination of ultra-low chamber pressure, high thermal heat transfer and a short primary-to-secondary phase transition reduces drying times by more than 80 % compared to conventional BFD. In a second step, a design of experiment approach was used to assess the effect of thermal radiation versus microwave radiation and their combination, together with dosage properties such as fill volume and excipient concentration upon drying rate. The outcome showed the importance of high fill volume and high excipient concentration for an effective microwave contribution to the drying rate. Nevertheless, the drying acceleration for small pharmaceutical dosages with restricted solutes was mainly driven by thermal radiation rather than 2.45 GHz microwave radiation. The inability of ice to convert microwave energy into heat hampers the potential use of microwave freeze-drying for single-dose vaccines.

Analysis of microwave heating of copper powder compacts

The influence of the native oxide films, which is essential for the understanding of the microwave heating of metal powder, is analyzed numerically based on the experimental data on temperature, dilatation and resistance of compacted copper powder samples. The indicative thickness of the oxide films, determined from the resistivity data using an effective medium approximation, is shown to decrease from ≥1 μm to below 1 nm. The effective complex dielectric permittivity and magnetic permeability of the copper powder with oxide films on the particles are calculated within the recently developed models. The dielectric permittivity exhibits a percolation behavior in the temperature range of the oxide decomposition, viz. 210–250 °C when microwave heating is carried out in nitrogen and 330–375 °C – in argon. The efficiency of absorption of the incident 30 GHz microwave radiation in a slab of powder is assessed separately for the electric and magnetic-type losses, the contribution of the latter being predominant until the percolation transition. The energy flux density of the incident microwave radiation required to sustain the prescribed heating rate is shown to increase during the microwave heating process from ∼20 W/cm2 to >1 kW/cm2 due to increasing reflection. The additional microwave energy input required for the completion of the endothermic oxide decomposition reaction is shown to be significant at the initial stage of the process (below 200 °C).

Doubly resonant metal-free electro-optic microwave receiver in aluminum nitride

This paper demonstrates a passive, integrated electro-optic receiver for detection of free-space microwave radiation. Unlike a traditional microwave receiver, which relies on conductive antennas and electrical amplifiers, this receiver uses only passive, optically probed elements with no electrodes or electronic components. The receiver employs two co-resonant structures: a dielectric resonator antenna (DRA) to concentrate incoming microwave radiation and an integrated aluminum nitride (AlN) racetrack resonator to resonantly enhance the optical carrier. The microwave field of the DRA modulates the built-up optical carrier in the resonator via the electro-optic response of AlN. We successfully detected 15 GHz microwave radiation through co-resonant electro-optic up-conversion, without the need for any conducting electrodes, amplifiers, or electronic components.

Metformin Ameliorates 2.856 GHz Microwave- Radiation-Induced Reproductive Impairments in Male Rats via Inhibition of Oxidative Stress and Apoptosis.

The reproductive system has been increasingly implicated as a sensitive target of microwave radiation. Oxidative stress plays a critical role in microwave radiation -induced reproductive damage, though precise mechanisms are obscure. Metformin, a widely used antidiabetic drug, has emerged as an efficient antioxidant against a variety of oxidative injuries. In the present study, we hypothesized that metformin can function as an antioxidant and protect the reproductive system from microwave radiation. To test this hypothesis, rats were exposed to 2.856 GHz microwave radiation for 6 weeks to simulate real-life exposure to high-frequency microwave radiation. Our results showed that exposure to 2.856 GHz microwave radiation elicited serum hormone disorder, decreased sperm motility, and depleted sperm energy, and it induced abnormalities of testicular structure as well as mitochondrial impairment. Metformin was found to effectively protect the reproductive system against structural and functional impairments caused by microwave radiation. In particular, metformin can ameliorate microwave-radiation-induced oxidative injury and mitigate apoptosis in the testis, as determined by glutathione/-oxidized glutathione (GSH/GSSG), lipid peroxidation, and protein expression of heme oxygenase-1 (HO-1). These findings demonstrated that exposure to 2.856 GHz microwave radiation induces obvious structural and functional impairments of the male reproductive system, and suggested that metformin can function as a promising antioxidant to inhibit microwave-radiation-induced harmful effects by inhibiting oxidative stress and apoptosis.

Disrupted Topological Organization of Brain Network in Rats with Spatial Memory Impairments Induced by Acute Microwave Radiation.

Previous studies have suggested that microwave (MW) radiation with certain parameters can induce spatial memory deficits. However, the effect of MW on the topological organization of the brain network is still unknown. This work aimed to investigate the topological organization of the brain network in rats with spatial memory impairments induced by acute microwave (MW) radiation. The Morris water maze (MWM) test and resting-state functional magnetic resonance imaging were performed to estimate the spatial memory ability and brain network topological organization of the rats after MW exposure. Compared with the sham group, the rats exposed to 30 mW/cm2 1.5 GHz MW radiation exhibited a significantly decreased normalized clustering coefficient (γ) (p = 0.002) 1 d after the exposure and a prolonged average escape latency (AEL) (p = 0.014) 3 d after the exposure. Moreover, after 10 mW/cm2 1.5 GHz MW radiation, a significantly decreased γ (p = 0.003) was also observed in the rats, without any changes in AEL. In contrast, no adverse effects on AEL or topological parameters were observed after 9.375 GHz MW radiation. In conclusion, the rats with spatial memory deficits induced by MW radiation exhibited disruptions in the topological organization of the brain network. Moreover, these topological organization disruptions emerged earlier than behavioral symptom onset and could even be found in the rats without a decline in the performance of the spatial memory task. Therefore, it is possible to use the topological parameters of the brain network as early and sensitive indicators of the spatial memory impairments induced by acute MW radiation.

Direct configuration mode microwave sintering of natural uranium dioxide pellets

The direct microwave sintering of natural uranium dioxide (UO2) pellets at bulk scale is first time successfully demonstrated. The process of the microwave sintering is carried out at 1700 °C, 6h in reducing atmosphere with a sintering cycle spanning over 31h in an indigenously designed and developed sintering set-up deploying 2.45 GHz microwave radiation. The process is demonstrated for obtaining defect-free pellets (devoid of cracks and any plasma induced edge/surface melting or burnouts) in bulk quantity consisting of a batch size of 3 kg (500–550 pellets). The sinter density of pellets was found to be uniform and in the acceptable range of 92–96%TD for nuclear fuel. The comparison of optical microstructures prima facie indicates that the UO2 grain size is smaller in microwave sintered pellets (5–20 μm) as compared to conventionally sintered UO2 pellets (10–40 μm) under identical parameters. The axial and the diameteral shrinkages during densification were observed to be 18–20% which are comparable to the conventional sintering. The microwave sintered UO2 pellets has O/U mole ratio as 2.002. The process is consistent and easily up-scalable to sinter large size of batches so as to meet the industrial scale demands.

The Effect of 2.45 GHz Electromagnetic Fields on Fear Memory Extinction in Male Rats.

Introduction: Fear memories are influenced by psychological and environmental variables. We evaluated the effect of 2.45 GHz microwave radiation on rats' fear learning and memory ability to determine the potential risks. The present study aimed to assess the impacts of corticosterone (CORT) levels on the consolidation and reconsolidation of fear conditioning memories. Methods: The rats were evaluated in contextual fear conditioning using foot shocks in both short-term (7 days) exposure and long-term (30 days) exposure. Young male Wistar rats were continually exposed to radio frequency electromagnetic field radiation for 5-6 weeks (1 h/day) with a frequency, power density, and pulse width of 2.45 GHz, 6.0 mW/cm2, and 2 ms, respectively. Several animals housed in identical conditions without exposure to radiation were monitored. Results: Based on the results, a significant increase and decrease in body weight and percentage of the freezing time were observed after the short-term group respectively. However, in the long term, we observed no significant difference in body weight, and the freezing time decreased substantially. Conclusion: As CORT levels were analyzed, long-term radiation might increase stress, which was associated with significant weight loss in rats.

2.45 GHz microwave radiation induced oxidative stress: Role of inflammatory cytokines in regulating male fertility through estrogen receptor alpha in Gallus gallus domesticus

Due to the growing number of gadgets emitting electromagnetic radiation (EMR), particularly microwave (MW) radiation, in our daily lives, it is believed that EMR have both long-term and short-term biological impacts that are quite concerning for avian as well as human health. Due to the negative impact of MW emitting equipment on the biological system this study looks into the mechanistic approach by which low-level of 2.45 GHz MW radiation causes an oxidative stress and inflammatory response in the testes micro-environment which further gets regulated by estrogen receptor alpha (ERα) expression in immature Gallus gallus domesticus leading to male infertility. Two weeks old immature male chickens were exposed to non-thermal low-level 2.45-GHz MW radiation for 2 h/day for 30 days (power density = 0.1264 mw/cm2 and SAR = 0.9978 W/kg). In the exposed group, morphometric examination of the testes revealed decreased testicular weight, volume and gonado-somatic index. Further, histological staining demonstrated a substantial reduction in the diameter of seminiferous tubules in the exposed group as compared to the control. The degree of oxidative stress was also determined showing an increase in oxidative stress parameters after exposure. The radiation exposed testes showed a significant increase in IL-1β immunoreactivity and decline in IL-10 immunoreactivity, indicating a sense of MW radiation-induced oxidative stress-regulated inflammatory response. A substantial reduction in ERα expression was also observed in exposed testes by Western blotting. Our investigations conclude that testes being vulnerable to free radical damage become an easy target organ for MW exposure induced oxidative and inflammatory stress. Therefore it becomes evident that it may cause male infertility in chicks via downregulation of ER-α in testis.

The potential adverse effect of 2.45 GHz microwave radiation on the testes of prenatally exposed peripubertal male rats.

In utero development of organs is easily influenced by many environmental factors. The aim of this study was to elucidate the effect of microwave radiation (MR) at a frequency of 2.45 GHz and a specific absorption rate of 1.73 W/kg on intrauterine development of testis. Pregnant albino rats were exposed to whole-body MR for 2 hours per day throughout the pregnancy. Male offspring (n=12, age 35 days) were not exposed to MR after birth. The study revealed that MR applied in utero induced apparent structural changes in the testes, such as irregular shape of seminiferous tubules, significant decrease in the diameter of seminiferous tubules (p<0.05) and in the height of the germinal epithelium (p<0.01), disorganisation of germ cells, desquamations of immature germ cells, formation of giant multinucleated cells, and significant (p<0.01) expansion of the interstitium. At the level of transmission electron microscopy, there were observed basement membrane irregularities in seminiferous tubules, vacuolation of the cytoplasm and adversely affected organelles in Sertoli cells, germ cells, Leydig cells, peritubular and endothelial cells. The tight junctions between adjacent Sertoli cells were often incomplete, and necrotizing germ cells were more numerous in experimental animals compared to controls. Enhanced necrotizations of germ cells proved by a Fluoro Jade C method, and declined germ cells proliferation confirmed by proliferating cell nuclear antigen analysis, were detected in MR exposed animals. Our results revealed that the prenatal exposure to MR had an adverse effect on the postnatal testicular development in rats.

Performance evolution of Fe3O4 used in the production of sustainable self-healing asphalt materials

Studies have shown that the sustainable self-healing ability of asphalt materials under microwave radiation is related to the microwave absorption of the constituent materials. As a typical absorbing material, Fe3O4 and its derivatives have the potential to heal the micro cracks of asphalt materials multiple times by themselves, and enhance the sustainable self-healing properties. However, former researchers have neglected the fact that Fe3O4 will be gradually oxidized at high temperatures, such as 100 °C, resulting in the changes in material properties, microwave absorption and heat-generating capability. Therefore, the effects of different temperatures on the properties of controllable synthesized Fe3O4 were studied at 125 °C (mixing temperature of WMA), 150 °C (mixing temperature of HMA), 175 °C (mixing temperature of HMA with modified binder) and 200 °C (extreme mixing temperature). The phase compositions, chemical structure of synthesized Fe3O4 were characterized firstly. Then, their electromagnetic parameters, reflection loss were studied at a microwave frequency ranging from 1 to 18 GHz. Additionally, heat-generating abilities of them were characterized at usual microwave frequency of 2.45 GHz. Finally, the effects of different content of Fe3O4 on the rheological properties of pure asphalt were studied. The results show that the proportion of γ-Fe2O3 in the samples gradually increase with exposure temperature, and the static magnetic properties gradually deteriorate. The reflection loss of the modified asphalt decreases firstly and then increases. The F-150 modified asphalt has the strongest microwave absorption capacity. Under the 2.45 GHz microwave radiation, the F-150 modified asphalt presents the strongest heat-generating capability and is consistent with the results of reflection loss. It can be seen that the ratio of γ-Fe2O3 and Fe3O4 in F-150 has the best attenuation effect on microwave radiation, and Fe3O4 is more suitable as microwave absorber for self-healing enhancement of HMA.

Chronic exposure to 2.45 GHz microwave radiation improves cognition and synaptic plasticity impairment in vascular dementia model

Purpose: In this study, we evaluated the effects of 2.45 GHz microwave radiation on cognitive dysfunction induced by vascular dementia (VaD). Methods: The VaD was induced by bilateral-common carotid occlusion (2-VO). The rats were divided into 4 groups including: control (n = 6), sham (n = 6), 2-VO (n = 8), and 2-VO + Wi-Fi (n = 10) groups. Wi-Fi modem centrally located at the distance of 25 cm from the animal's cages and the animals were continuously exposed to Wi-Fi signal while they freely moved in the cage (2 h/day for forty-five days). Therefore, the power density (PD) and specific absorption rate value (SAR) decreased at a distance of 25 to 60 cm (PD = 0.018 to 0.0032 mW/cm2, SAR = 0.0346 to 0.0060 W/Kg). The learning, memory, and hippocampal synaptic-plasticity were evaluated by radial arm maze (RAM), passive avoidance (PA), and field-potential recording respectively. The number of hippocampal CA1 cells was also assessed by giemsa staining. Results: Our results showed that VaD model led to impairment in the spatial learning and memory performance in RAM and PA that were associated with long-term potentiation (LTP) impairment, decrease of basal-synaptic transmission (BST), increase of GABA transmission, and decline of neurotransmitter release-probability as well as hippocampal cell loss. Notably, chronic Wi-Fi exposure significantly recovered the learning-memory performance, LTP induction, and cell loss without any effect on BST. Conclusions: The LTP recovery by Wi-Fi in the 2-VO rats was probably related to significant increases in the hippocampal CA1 neuronal density, partial recovery of neurotransmitter release probability, and reduction of GABA transmissiSon as evident by rescue of paired-pulse ratio 10 ms.

The use of microwave radiation with a frequency of 2.45 GHz as a factor reducing the storage losses of potato tubers

The goal of the paper is to evaluate the suitability of the 2.45 GHz microwave radiation in reducing the potato tubers storage losses. The experiment was conducted for three consecutive storage seasons on six edible potato varieties. It was determined that reaction of potato tubers to microwave radiation defined by processes taking place during the storage has a varietal character. Some microwave-irradiated combinations showed tendencies for increased germination. One potato variety responded to microwave radiation with significantly lower degree of infection by black scurf.

Studies on Physiological Effects and Plasmid Stability of JM109 Host System

Objective: The purpose of the study delineates the growth and plasmid stability of E. coli JM109 host system.&#x0D; Methods: Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability.&#x0D; Results: Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, dantron showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth was observed in exposure to UV rays. Conclusion: The present work discussed that, the effect of drugs, chemicals, radio frequency and microwave radiation have a huge effect not only on growth of organism but also concentration and stability of plasmid.

Gene expression of certain heat shock proteins and antioxidant enzymes in microwave exposed rats

Cellular interaction between microwave radiations 2.45GHz frequency and cellular function is very important in explaining the role played by the cellular signaling pathway in keeping cell function within normal condition. The aim of this study is to examine the gene expression of certain heat shock proteins and antioxidant enzymes in microwave exposed rats. Thirty female rats at the age of three months were divided into two groups: control and exposed groups, whole body exposure for 2 h/day up to 60 days at SAR value 0.1434 W/Kg were employed. On the day 60 of experiment, liver samples were collected from scarified animals for RNA isolation and heat shock protein and antioxidant enzymes genes expression by RT-qPCR. The results showed up and down regulations of the studied genes in exposed rats compared to control rats. Also, there is a strong linear relationship between antioxidant enzymes and heat shock protein expression; there is a strong linear relationship between (catalase and GST) genes and GSH-Px1gene expression. The study concludes that the 2.45GHz microwave radiation induced alteration in certain heat stress proteins and antioxidant enzymes gene expression at the cellular level as a consequence of cellular toxicity resulted from oxidative stress induction.

Microwave plasma enhancement of multiphase flames: On-demand control of solid propellant burning rate

This effort explores the microwave-supported plasma enhancement of an aluminized, ammonium perchlorate composite solid propellant flame. The technique is enabled through novel alkali metal doping, which provides increased levels of ionization and allows efficient microwave energy deposition to the flame structure and subsequent perturbation of the steady-state propellant burning rate. Three potential modes of energy deposition are identified in composite propellants: (1) plasma-enhancement promoted by the presence of sodium, (2) strong absorption by high temperature metallic oxide products, and (3) direct absorption of energy by the propellant burning surface condensed phase. Equilibrium calculations are used to identify propellant compositions with high free-electron populations, and solution of the Boltzmann equation using the BOLSIG + code is used to show the significant effects of sodium doping on microwave absorption of the flame. Experimental emission spectroscopy and imaging of the microwave-enhanced propellant flame structure show evidence of these mechanisms. Propellant burning rates can be increased by up to 60% through the application of 1 kW, continuous 2.46 GHz microwave radiation. Propellant doping with 3.5% by weight of sodium nitrate shows significant improvement in the effectiveness of microwave application in modifying the propellant burning rate. This technique provides high levels of dynamic propellant combustion control from a solid-state system. The on-demand control of energetic material burning rates through low level doping and microwave irradiation is a promising technique which could lead to the development of a new class of ‘smart’ dynamically controllable energetic materials.

2.45 GHz microwave radiation impairs learning, memory, and hippocampal synaptic plasticity in the rat.

Microwave (MW) radiation has a close relationship with neurobehavioral disorders. Due to the widespread usage of MW radiation, especially in our homes, it is essential to investigate the direct effect of MW radiation on the central nervous system. Therefore, this study was carried out to determine the effect of MW radiation on memory and hippocampal synaptic plasticity. The rats were exposed to 2.45 GHz MW radiation (continuous wave with overall average power density of 0.016 mW/cm2 and overall average whole-body specific absorption rate value of 0.017 W/kg) for 2 h/day over a period of 40 days. Spatial learning and memory were tested by radial maze and passive avoidance tests. We evaluated the synaptic plasticity and hippocampal neuronal cells number by field potential recording and Giemsa staining, respectively. Our results showed that MW radiation exposure decreased the learning and memory performance that was associated with decrement of long-term potentiation induction and excitability of CA1 neurons. However, MW radiation did not have any effects on short-term plasticity and paired-pulse ratio as a good indirect index for measurement of glutamate release probability. The evaluation of hippocampal morphology indicated that the neuronal density in the hippocampal CA1 area was significantly decreased by MW.

2.45 GHz microwave radiation induced oxidative and nitrosative stress mediated testicular apoptosis: Involvement of a p53 dependent bax-caspase-3 mediated pathway.

Deleterious effects of MW radiation on the male reproduction are well studied. Previous reports although suggest that 2.45 GHz MW irradiation induced oxidative and nitrosative stress adversely affects the male reproductive function but the detailed molecular mechanism occurring behind it has yet to be elucidated. The aim of present study was to investigate the underlying detailed pathway of the testicular apoptosis induced by free radical load and redox imbalance due to 2.45 GHz MW radiation exposure and the degree of severity along with the increased exposure duration. Twelve-week old male mice were exposed to 2.45 GHz MW radiation [continuous-wave (CW) with overall average Power density of 0.0248 mW/cm2 and overall average whole body SAR value of 0.0146 W/kg] for 2 hr/day over a period of 15, 30, and 60 days. Testicular histology, serum testosterone, ROS, NO, MDA level, activity of antioxidant enzymes, expression of pro-apoptotic proteins (p53 and Bax), anti-apoptotic proteins (Bcl-2 and Bcl-xL ), cytochrome-c, inactive/active caspase-3, and uncleaved PARP-1 were evaluated. Findings suggest that 2.45 GHz MW radiation exposure induced testicular redox imbalance not only leads to enhanced testicular apoptosis via p53 dependent Bax-caspase-3 mediated pathway, but also increases the degree of apoptotic severity in a duration dependent manner.

Microwave radiation alters burn injury-evoked electric potential in Nicotiana benthamiana

ABSTRACTThe dielectric effect enforced on charged ions and dipolar molecules by the oscillating electric field of microwaves may influence electric signaling in plants. In the present study, the exposure of Nicotiana benthamiana plants to continuous wave 2.45 GHz microwave radiation with 1.9 – 2.1 W m−2 power density significantly reduced the amplitude of leaf burning-induced variation potential along the plant stem. The change in amplitude of the variation potential occurred mainly because of a significant reduction of the depolarization rate. This effect was not observed during the post-microwave exposure period. The unique characteristics observed in the variation potentials were also observed under microwave exposure, suggesting unaffected information delivery to distant locations or unaffected transport of specific chemicals generated by the injury.

Effect of 2.45 GHz microwave radiation on the fertility pattern in male mice.

A number of studies have reported that male reproductive organs are susceptible to electromagnetic fields (EMFs). The aim of this study was to explore the effects of microwave radiation exposures on 6-8 weeks old male Swiss albino mice. Mice were divided into two groups: group I - sham-exposed (Control), and group II - microwaves-exposed (MWs). Mice were exposed to 2.45 GHz with power density 0.25 mW/cm2 and specific absorbtion rate (SAR) 0.09 W/kg. Exposure was given in Plexiglas cages for 2 h/day for 30 days. Results showed that 2.45 GHz microwave radiation resulted in a significant increase (p < 0.001) in catalase (CAT), malondialdehyde (MDA), reactive oxygen species (ROS) and decrease (p < 0.001) in the levels of glutathione peroxidase (GPx), testosterone and superoxide dismutase (SOD) (p < 0.05). Flow cytometer analysis of blood showed the formation of micronuclei in microwave-exposed group. Histopathological changes were also seen in seminiferous tubules of microwave-exposed group as compared to the sham-exposed group. It is concluded that 2.45 GHz microwave radiation exposure causes oxidative stress in testes and it may lead to detrimental and injurious effects on fertility potential of the male reproductive system of Swiss albino mice.

Development of quantum voltage noise source chip for precision measurement of Boltzmann constant

The Johnson noise thermometer is used to precisely measure Boltzmann constant by comparing the thermal noise caused by charge movement and the quantized voltage reference noise synthesized by the quantum voltage noise source (QVNS). The QVNS signal is synthesized based on quantized voltage pulses produced by two channels of superconducting Josephson junction arrays, which are designed for cross-correlation electronics. The Nb/NbxSi1-x/Nb Josephson junction is used as a core device of QVNS chip in this work for its non-hysteretic current-voltage (I-V) characteristics and conveniently adjustable barrier parameters.In this paper, we present the design consideration, fabrication process, and measurement results of the QVNS chip. The QVNS chip contains two Josephson junction arrays, each consists of four 6 μm×12 μm junctions and is embedded in a 50 Ω coplanar waveguide transmission line. The random noise in signals from the two driven channels is eliminated by cross-correlation, and then an accurate quantum noise is obtained. Test chips with different areas of Josephson junctions are also designed on the same mask, aiming at estimating the variation range of Ic. The typical fabrication process for voltage standard chips in our laboratory is used for preparing the QVNS chip.The sample is measured at 4.2 K. The DC I-V curve shows that the critical current Ic is 6.1 mA. The I-V characteristics of the junctions under 5 GHz microwave radiation are measured. For a series array of four junctions, a 41.44 μV one-stage Shapiro step is observed. Calculation shows that the error between the measurement and theoretical value of 41.36 μV is about 1.9‰, which means that the QVNS chip performs well under microwave radiation and can be used for synthesizing the AC quantum voltage reference noise.A single-frequency 100 kHz sinusoidal waveform is synthesized by the QVNS chip under pulse driven signal. A spectrum of the synthesized sinusoidal waveform shows a single peak, which means that the digital pulse signal is perfectly filtered by Josephson junction arrays and the synthesized signals possess quantum accuracy. The results indicate that our chip has good dynamic response and works well in synthesizing a single-frequency AC quantum voltage signal. This work can provide core devices for the noise thermometry system and support the precise measurement of Boltzmann constant as well as redefinition of Kelvin in future. As a next step, the design and package will be further improved, and the probe module will be optimized to reduce the measurement uncertainty.