Ghrelin Research Articles

Voluntary exercise is motivated by ghrelin, possibly related to the central reward circuit.

We previously reported that voluntary exercise contributed to the amelioration of abnormal feeding behavior with a concomitant restoration of ghrelin production in a rat model of obesity, suggesting a possible relationship between exercise and appetite-regulating hormones. Ghrelin is known to be involved in the brain reward circuits via dopamine neurons related to motivational properties. We investigated the relevance of ghrelin as an initiator of voluntary exercise as well as feeding behavior. The plasma ghrelin concentration fluctuates throughout the day with its peak at the beginning of the dark period in the wild-type (WT) mice with voluntary exercise. Although predominant increases in wheel running activity were observed accordant to the peak of plasma ghrelin concentration in the WT mice, those were severely attenuated in the ghrelin-knockout (GKO) mice under either ad libitum or time-restricted feeding. A single injection of ghrelin receptor agonist brought about and reproduced a marked enhancement of wheel running activity, in contrast to no effect by the continuous administration of the same drug. Brain dopamine levels (DAs) were enhanced after food consumption in the WT mice under voluntary exercise. Although the acceleration of DAs were apparently blunted in the GKO mice, they were dramatically revived after the administration of ghrelin receptor agonist, suggesting the relevance of ghrelin in the reward circuit under voluntary exercise. These findings emphasize that the surge of ghrelin plays a crucial role in the formation of motivation for the initiation of voluntary exercise possibly related to the central dopamine system.

GHRL gene-based genotyping of ovine and caprine breeds reveals highly polymorphic intronic sequences in Awassi sheep with several RNA motifs

BackgroundThe current study was conducted to identify the genetic polymorphism of ghrelin (GHRL) gene of sheep and goats, as well as to determine whether these polymorphisms were associated with the evolutionary genetic differences in the involved species. This study was performed on 233 sheep and 91 goats. Two genetic loci of 113 bp and 262 bp partially spanning over exon 2/intron 2 and intron 4/exon 5 of GHRL gene respectively were amplified and genotyped using polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods. ResultsThe SSCP banding pattern of 262-bp locus indicated the presence of four diplotypes (BC, BB, AC, and AB) in Awassi sheep, three diplotypes (BC, BB, and AB) in Karadi sheep, and only two diplotypes (BC and BB) in all goats’ samples. The current study detected several novel SNPs in the ovine–caprine populations as well as two SNPs that are observed only in sheep, including intron4:119 C>A and intron4:123 T>G. The phylogenetic analysis revealed that the observed diplotypes resided within ovine sequences and were closely related to caprine counterparts. Computational analyses indicated the presence of various intronic RNA motifs. However, all these motifs were gathered in Awassi breed. ConclusionIt is stated that the intron 4 is highly diverse amongst goats and sheep as well as within sheep with a particular emphasis on Awassi. This genetic peculiarity may in turn suggest a high polymorphic pattern of this breed in comparison with other related counterparts.

여성 발레무용수의 스포츠 영양지식과 식욕조절 호르몬들 간의 관계

여성 발레무용수의 스포츠 영양지식과 식욕조절 호르몬들 간의 관계

Research of Jiawei-Xiaoyao powder on the Ghrelin, VIP for the non-erosive reflux disease with stagnation of liver Qi and spleen deficiency type

Objective To investigate the effect of Jiawei-Xiaoyao powder on the levels of somatotropin and vasoaetive intestinal peptide (VIP) in patients with non-erosive reflux disease (NERD). Methods Seventy patients with liver-stagnation and spleen-deficiency NERD who met the inclusion criteria were divided into two groups according to the random number table, 35 in each group. The treatment group was orally administered with Jiawei-Xiaoyao powder, and the control group was given oral omeprazole enteric-coated tablets and domperidone. Both groups were treated for 8 weeks. Plasma somatotropin and VIP levels were measured by ELISA. Results After treatment, plasma somatotropin levels (210.96 ± 16.85 pg/ml vs. 195.75 ± 12.62 pg/ml, t=4.274) in the treatment group were significantly higher than that in the control group (P<0.01), and plasma VIP level (16.12 ± 1.41 pg/ml vs. 18.57 ± 2.68 pg/ml, t=4.786) in the treatment group was significantly lower than that in the control group (P<0.01). Conclusions The Jiawei-Xiaoyao powder could reduce plasma VIP level in NERD patients, increase plasma somatotropin levels of NERD. Key words: Jia Wei Xiao Yao San; Non-erosive reflux disease; Syndrome of stagnation of liver qi and spleen deficiency; Somatotropin; Vasoactive intestinal peptide

Dietary Intake and Appetite Hormone Patterns among Mothers Participating in the Supplemental Nutrition Assistance Program: A Pilot Study

ABSTRACT Supplemental Nutrition Assistance Program (SNAP) participation has been associated with obesity in women. The objective of this pilot study was to describe the caloric intake and dietary quality at the beginning, middle, and end of a SNAP cycle among food insecure, African-American female head-of-households with overweight/obesity (n = 12). The feasibility of obtaining appetite-regulating hormones to assess the associations between appetite regulation and caloric intake was explored as a secondary aim. Mean caloric intake and dietary quality, as measured by HEI-2010 score, did not differ among study visits. Four patterns of caloric intake among the twelve subjects were reported in which all subjects reported caloric intake less than their daily requirements for weight maintenance. Appetite-stimulating hormone, neuropeptide Y concentrations, was positively associated with percent of Estimated Energy Requirements (%EER) consumed.

Association of Ghrelin Gene Polymorphisms with Fattening Traits and Feed Intake in Pig: A Preliminary Study.

Simple SummaryFrom a production point of view, feed intake, growth and carcass quality are among the most important traits in pig breeding. Understanding the mechanisms and processes going on inside the animal’s body can help in the selection of herds and improvement in economic effectiveness. Previous research conducted on different species has showed that ghrelin (GHRL) is responsible for feed intake, efficiency of growth, etc. Thus, evaluation of the regulatory regions and coding sequence of the porcine GHRL gene may be useful as a molecular marker for selected fattening and feed efficiency traits. In this paper, a promising mutation at the locus g.4486C>T was found, which was associated with total feed intake. Numerous studies have been conducted to explain the biological functions and mechanism of ghrelin (GHRL) action in animals. However, the exact role of ghrelin in the regulation of growth and development in pigs is still unclear. The ghrelin gene is considered to be a good candidate marker for the identification of economically important traits in pig production such as feed intake, growth or carcass quality. The objectives of the present study were to investigate the regulatory regions and coding sequence of the porcine GHRL gene and determine the effect of polymorphisms at the ghrelin gene locus on selected fattening traits. Data were obtained from 346 gilts (pure breeds: Landrace, 188; Duroc, 74; Pietrain, 84). The PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method was used to detect polymorphisms within GHRL. Three polymorphisms were found, one in the promoter region (c.-93A>G) and two in the 3’UTR sequence (g.4428T>C; g.4486C>T). A significant (p ≤ 0.01) additive effect on daily gain (negative) and age at slaughter (positive) was obtained at the locus c.-93A>G. However, the most promising mutation was at the locus g.4486C > T, which is associated with total feed intake. Overall, the described GHRL polymorphisms may be useful as molecular markers in pig selection but future studies are required.

Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs

The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin receptor (GHSR), LEP, leptin receptor (LEPR), SST and CCK) by reverse transcription-qPCR (RT-qPCR). TP generally had higher mRNA expressions of GHSR, LEP, LEPR, SST and CCK genes compared to YP, and expressed lower levels of the GHRL gene in most tissues of the digestive tract. In both breeds, plasma levels of the expressed proteins were more closely correlated with the feed intake and growth than with mRNA levels of the target genes. Our data indicate that TP possess special gene expression patterns in the gastrointestinal tract compared to YP, which is consistent with its unique feed intake and adaptation to harsh environment.

Effects of fasting on the central expression of appetite-regulating and reproductive hormones in wild-type and Casper zebrafish (Danio rerio)

Appetite and reproduction are closely related functions that are both regulated by brain hormones. Appetite stimulators include orexin and neuropeptide Y (NPY), and reproductive hormones include gonadotropin-releasing hormone (GnRH), gonadotropin-inhibitory hormone (GnIH), kisspeptin, and neurokinin B (NKB). GnRH stimulates the secretion of pituitary gonadotropes, and kisspeptin and GnIH modulate this action. Kisspeptin secretion is further controlled by neurokinin B (NKB) and dynorphin A (Dyn). To better understand the mechanisms regulating appetite and reproduction in fish, we examined the effects of fasting, reproductive stage, gender, and strain on the brain mRNA expression of appetite (orexin and NPY) and reproductive (GnRH, kisspeptin, GnIH, and NKB) hormones in zebrafish. In order to compare strains, we used both wild-type and transparent Casper zebrafish. In female wild-type zebrafish, fasting increased the expression of all hormones investigated, with the exception of Kiss2. Only NPY and Kiss2 were increased in male wild-type zebrafish during fasting. In Casper zebrafish, only GnIH and NKB in males were affected by fasting, suggesting that Casper fish may be more resistant to fasting than wild fish. Fasting increased expressions of orexin, GnRH2, Kiss1, GnIH and NKB in wild-type females with more eggs or larger eggs relative to body weight, compared to those with fewer or smaller eggs, suggesting that more mature females are more affected by fasting. No significant interactions of fasting and reproductive stage were noted in female Casper fish. To investigate whether differences between Casper and wild-type fish were due to genes involved in pigmentation, we compared the brain mRNA expressions of enzymes involved in melanin synthesis (tyrosinase and tyrosine hydroxylase – TH), melanocortin receptors (MC3R and MC4R), and the melanocortin precursor (proopiomelanocortin – POMC) between the two strains. Casper zebrafish had lower levels of MC3R, tyrosinase, TH1, TH2, and POMC than wild-type fish. Overall, our results suggest the existence of gender- and reproductive stage-specific, as well as strain-specific variations in the mechanisms regulating feeding and reproduction in zebrafish, and that the melanocortin system and melanin pathways may be in part responsible for these differences between strains.

Regulation of Gastrointestinal Motility by Motilin and Ghrelin in Vertebrates.

The energy balance of vertebrates is regulated by the difference in energy input and energy expenditure. Generally, most vertebrates obtain their energy from nutrients of foods through the gastrointestinal (GI) tract. Therefore, food intake and following food digestion, including motility of the GI tract, secretion and absorption, are crucial physiological events for energy homeostasis. GI motility changes depending on feeding, and GI motility is divided into fasting (interdigestive) and postprandial (digestive) contraction patterns. GI motility is controlled by contractility of smooth muscles of the GI tract, extrinsic and intrinsic neurons (motor and sensory) and some hormones. In mammals, ghrelin (GHRL) and motilin (MLN) stimulate appetite and GI motility and contribute to the regulation of energy homeostasis. GHRL and MLN are produced in the mucosal layer of the stomach and upper small intestine, respectively. GHRL is a multifunctional peptide and is involved in glucose metabolism, endocrine/exocrine functions and cardiovascular and reproductive functions, in addition to feeding and GI motility in mammals. On the other hand, the action of MLN is restricted and species such as rodentia, including mice and rats, lack MLN peptide and its receptor. From a phylogenetic point of view, GHRL and its receptor GHS-R1a have been identified in various vertebrates, and their structural features and various physiological functions have been revealed. On the other hand, MLN or MLN-like peptide (MLN-LP) and its receptors have been found only in some fish, birds and mammals. Here, we review the actions of GHRL and MLN with a focus on contractility of the GI tract of species from fish to mammals.

The effects of exercise and ambient temperature on dietary intake, appetite sensation, and appetite regulating hormone concentrations

BackgroundIt is not clear whether the frequently reported phenomenon of exercise-induced anorexia is exacerbated or blunted in warm or cold environments. Therefore, this study investigated the effects of exercise in three different environmental temperatures vs. rest, on perceptions of appetite, appetite regulating hormones, and food intake.MethodsIn a randomized repeated-measures design, 18 Canadian Armed Forces members (14 male, 4 female) completed four 8-h trials in a thermally-controlled chamber: one 8-h resting trial at 21 °C (Sedentary); and three trials where participants completed two 2-h circuits of standardized military tasks interspersed with two 2-h rest periods, once at 30 °C (Hot), once at 21 °C (Temperate), and once at − 10 °C (Cold). Participants consumed military field rations ad libitum and had their appetite assessed with visual analogue scales. Plasma concentrations of GLP-1, PYY, acylated ghrelin, and leptin were also determined.ResultsAppetite was perceived as being suppressed in the heat compared to the cold (p < 0.05). While neither exercise nor environmental temperature altered circulating GLP-1 levels, exercise in all environments increased blood concentrations of PYY (p < 0.05). Leptin concentrations were elevated in the heat and diminished in the cold (p < 0.05), and acylated ghrelin concentrations were affected by both exercise and ambient temperature resulting in Sedentary = Cold>Temperate = Hot (p < 0.05). Contrary to the changes in appetite perceptions and hormonal concentrations, dietary intake was not different between conditions (p > 0.05). Relative energy intake (total 24 h energy intake minus 24 h energy expenditure) on the other hand, was significantly higher during the Sedentary condition than it was during any of the active conditions (p < 0.05). Most (83%) of the participants were in a positive energy balance during the Sedentary condition, whereas during most (80%) of the active conditions (Hot, Temperate, Cold) participants were in a negative energy balance.ConclusionsIn this study where food was freely available, variations in ambient temperature, exercise vs. rest, appetite-regulating hormone concentrations, and subjective appetite sensation were not associated with any changes in dietary intake within 24-h of acute, prolonged exercise.

Obestatin/Ghrelin Ratio as a New Activity Index in Ulcerative Colitis

Background and Aim: Ulcerative colitis (UC) is an immune-mediated systemic inflammatory process that destroys the intestinal mucosa. Ghrelin, an appetite-regulatory hormone, has anti-inflammatory effects including a decrease in circulating cytokines. Some reports demonstrated a strong relationship between the serum ghrelin level and the severity of mucosal inflammation in the gastrointestinal tract. The aim is to investigate serum levels and colonic mucosal mRNA expression of ghrelin, obestatin, and obestatin/ghrelin ratio in patients with UC and to determine their potential as markers for UC disease activity. Patients and Methods: seventy-five outpatients with UC and 45 age- and sex-matched healthy volunteers were enrolled in this study after written conscious consent and approval by the Institutional Review Board of Mansoura University. UC was diagnosed by conventional clinical, radiological, endoscopic, and histopathological criteria. Serum ghrelin, obestatin levels, and their mucosal mRNA expression were measured by ELISA kits and a real-time quantitative reverse transcriptase polymerase chain reaction according to the manufacturer’s protocols. Results: Serum levels and mucosal mRNA expression of ghrelin were significantly higher in patients with active UC than patients in remission p˂0.0001). Obestatin/ghrelin ratio was significantly lower in patients with active UC (0.26±0.08) than those in remission (0.523±0.16; p˂0.0001). Obestatin/ghrelin ratio was negatively and significantly correlated with inflammation and endoscopic scores, colitis activity index, serum ghrelin level, and its mucosal mRNA expression (p˂0.05). Conclusion: obestatin/ghrelin ratio might be a reliable surrogate non-invasive marker of disease activity in UC with significantly high sensitivity, specificity, predictive values, and diagnostic accuracy.

Effect of ghrelin on the apoptosis of various cells. A critical review.

Ghrelin is a protein hormone secreted from the gastric mucosa, but is also expressed in a variety of tissues. The biological function of ghrelin is widely known, including regulation of food intake, body weight, gastrointestinal, cardiovascular, reproductive, immune functions, cell proliferation and hormone secretion. The growth hormone secretagogue receptor (GHS-R) mediates the effect of ghrelin. Depending on cell type, ghrelin either induces apoptosis or has anti-apoptotic effect. Apoptosis is a physiological process that plays an important role in many biological actions such as proliferation, development or differentiation and regulation of physiological function in many cells. Dysfunction or dysregulation of apoptosis leads to pathology conditions. The action of ghrelin on cell apoptosis is reviewed in this paper.

Laparoscopic sleeve gastrectomy induces molecular changes in peripheral white blood cells

Peripheral white blood cells (PWBC) may allow for the development of obesity biomarkers. We aimed to investigate the existence of gene expression and DNA methylation changes in PWBC after a very low calorie diet (VLCD) followed by a laparoscopic sleeve gastrectomy (LSG), and its correlation with surgical outcomes. From July 2013 to June 2014, 35 consecutive bariatric patients and 33 healthy lean volunteers were recruited. Molecular data was obtained once on the control group and at 3 different times on the LSG group: 1) at baseline; 2) after 2 weeks of VLCD, right before LSG; and 3) 6 months after LSG. The expression of 12 genes in PWBC was analyzed by quantitative real-time polymerase chain reaction: ghrelin (GHRL), visfatin (NAMPT), insulin receptor substrate 1 (IRS1), fat mass and obesity-related gene (FTO), leptin (LEP), peroxisome proliferator-activated receptor gamma (PPARG), adiponectin (ADIPOQ), fatty acid synthase (FASN), melanocortin 4 receptor (MC4R), fas cell surface death receptor (FAS), tumor necrosis factor alpha (TNF) and chemokine (C-C motif) ligand 2 (CCL2). Moreover, DNA methylation of GHRL, NAMPT and FAS promoters was analyzed in PWBC by bisulfite pyrosequencing. Seven genes (GHRL, NAMPT, IRS1, FTO, FAS, TNF and CCL2) had detectable expression in PWBC. FTO expression at baseline was lower in patients than in controls (p=0.042), equalizing after LSG. In patients, FAS expression decreased after VLCD (p=0.01) and stayed low after LSG (p=0.015). Also, CCL2 expression decreased 50% after LSG compared to pre-surgical levels (p=0.016). All studied CpG sites in the GHRL gene promoter followed a consistent pattern of DNA methylation/demethylation. No direct correlation between these molecular changes and surgical outcomes was found at 1-year follow-up. FTO expression increased and FAS and CCL2 expression decreased in PWBC after LSG. Molecular changes did not correlate with surgical outcomes.

발레무용수의 Lean Body Mass와 Appetite-Regulating Hormones의 상관관계

발레무용수의 Lean Body Mass와 Appetite-Regulating Hormones의 상관관계

The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia.

The physiological control of appetite regulation involves circulating hormones with orexigenic (ghrelin) and anorexigenic (cholecystokinin) properties that induce alterations in energy intake via perceptions of hunger and satiety. We sought to investigate the relationship between appetite-regulating hormones and the cachexia associated with chronic heart failure.We randomized male Sprague-Dawley rats into myocardial infarction (MI) or sham operation (SO) groups. The levels of brain natriuretic peptide (BNP), cholecystokinin (CCK) and ghrelin in the plasma of all rats were detected by enzyme-linked immunosorbent assay (ELISA); the expression of BNP, CCK, and ghrelin in the myocardial tissue of all rats were detected by western blotting, immunohistochemistry, real-time polymerase chain reaction (PCR); myocardial morphology was assessed by microscopy.Plasma BNP and CCK levels in the cardiac cachexia (CC) groups and the heart failure non-cachexia (HF-nc) groups were significantly higher than those in the control groups (P < 0.01), and the expression of BNP and CCK in the myocardial tissue of rats: in CC groups and HF-nc groups were increased compared with the corresponding control groups (P < 0.01). In contrast, Plasma and cardiac expression of ghrelin decreased compared with the sham group (P < 0.01). Furthermore, plasma CCK levels were positively correlated with BNP concentrations (P < 0.001) and significantly negatively correlated with the ejection fraction (P < 0.001) in model animals; plasma ghrelin levels were negatively associated with BNP levels (P = 0.0023) and positively associated with ejection fraction (P = 0.0042).The appetite-regulating hormones (ghrelin and CCK) may present as a potential significant biomarker for cachexia associated with chronic heart failure.

Mechanisms of Acupuncture Therapy for Simple Obesity: An Evidence-Based Review of Clinical and Animal Studies on Simple Obesity.

Simple obesity is a worldwide epidemic associated with rapidly growing morbidity and mortality which imposes an enormous burden on individual and public health. As a part of Traditional Chinese Medicine (TCM), acupuncture has shown the positive efficacy in the management of simple obesity. In this article, we comprehensively review the clinical and animal studies that demonstrated the potential mechanisms of acupuncture treatment for simple obesity. Clinical studies suggested that acupuncture regulates endocrine system, promotes digestion, attenuates oxidative stress, and modulates relevant molecules of metabolism in patients of simple obesity. Evidence from laboratory indicated that acupuncture regulates lipid metabolism, modulates inflammatory responses, and promotes white adipose tissue browning. Acupuncture also suppresses appetite through regulating appetite regulatory hormones and the downstream signaling pathway. The evidence from clinical and animal studies indicates that acupuncture induces multifaceted regulation through complex mechanisms and moreover a single factor may not be enough to explain the beneficial effects against simple obesity.

Ghrelin Binding at its G Protein-Coupled Receptor

Ghrelin Binding at its G Protein-Coupled Receptor

Serum concentration of appetite-regulating hormones of mother-infant dyad according to the type of feeding.

Satiety and appetite‐stimulating hormones play a role in the regulation of food intake. Breastfed infants may have a different profile of serum appetite‐regulating hormones than formula‐fed infants. We propose to demonstrate that the serum concentration of appetite regulatory hormones differs according to the type of feeding and that there is a correlation between the serum concentrations of these hormones in mothers and in infants at 4 months of age. In a cross‐sectional analysis, 167 mother–newborn dyads at the Hospital Civil de Guadalajara were enrolled: 74 full breastfeeding (FBF), 56 partial breastfeeding (PBF), and 37 receiving human milk substitutes (HMS). Serum levels of ghrelin (pg/ml), leptin (ng/ml), peptide YY (pg/ml), and glucagon‐like peptide‐1 (GLP‐1) (pM) were measured. We performed one‐way analysis of variance, unpaired Student t test, post hoc Tukey test, and Pearson correlation. The total sample at 16 weeks postpartum included 167 dyads. The mean age was 16 ± 1 weeks. The concentrations of GLP‐1 (pM) and peptide YY (pg/ml) were higher in the FBF group (42.6 and 442.9) than in the HMS group (35.2 and 401.9), respectively, p = 0.046 and p = 0.056. And, the FBF group had higher correlation coefficients of ghrelin (r = 0.411 vs. 0.165), GLP‐1 (r = 0.576 vs. 0.407), and peptide YY (r = 0.218 vs. 0.067), respectively, than the HMS group. The concentrations of GLP‐1 and peptide YY were higher in the FBF group when compared with the HMS group. Mother–infant dyads fed by FBF had more significant direct correlations of appetite‐regulating hormones than those who received HMS.

Two weeks of exercise training intensity on appetite regulation in obese adults with prediabetes.

No short-term exercise data exist testing whether training intensity modifies hormonal and perceived appetite in obese adults with prediabetes. Therefore, we compared the effects of short-term moderate-continuous (CONT) vs. high-intensity interval (INT) training on appetite regulation. Twenty-eight obese adults [age: 61.3 ± 1.5 yr; body mass index (BMI): 33.2 ± 1.1 kg/m2] with prediabetes were randomized to work-matched CONT ( n = 14) or INT ( n = 14) training for 2 wk. Plasma acylated ghrelin (AG), des-acylated ghrelin (dAG), active glucagon-like peptide-1 (GLP-1), and insulin were measured at 0, 30, and 60 min of a 75-g oral glucose tolerance test (OGTT) before and after training. Visual analog scales were administered at 0 and 120 min during the OGTT to examine perceived appetite. Three-day food logs were collected before and after testing to assess ad libitum diet. CONT and INT increased peak oxygen consumption ( P < 0.01) and decreased BMI ( P < 0.01). Although neither intervention altered fasting levels of AG ( P = 0.94), dAG ( P = 0.36), or insulin ( P = 0.67), CONT raised GLP-1 compared with INT ( P = 0.05). Exercise training did not affect postprandial suppression of AG ( P = 0.81) and dAG ( P = 0.67) or stimulation of GLP-1 ( P = 0.67) and insulin ( P = 0.32). Both interventions tended to decrease total energy and protein intake ( P = 0.09 and P = 0.05, respectively), despite no change in fasting hunger ( P = 0.88) and reduced perceived fullness at 120 min during the OGTT ( P = 0.05). We conclude that 2 wk of exercise training intensity does not modulate appetite-regulatory hormones in obese adults with prediabetes. Although perceived fullness to the OGTT was reduced after exercise, CONT and INT decreased energy intake, suggesting that exercise does not elicit compensatory appetite behavior to gain weight. NEW & NOTEWORTHY Adults with prediabetes are at risk for appetite dysregulation. Although exercise promotes weight management, it is unclear whether moderate-continuous or high-intensity interval training is more beneficial for appetite regulation. We show that 2 wk of exercise, independent of intensity, does not alter postprandial appetite hormones or hunger, despite slight reductions in food intake and weight. These data support exercise as an effective method to induce negative energy balance without compensatory weight gain.

Polymorphism Analysis of GHRL Gene in Goat

Polymorphism Analysis of GHRL Gene in Goat