Stimulation Of Germination Research Articles

Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha.

KARRIKIN INSENSITIVE2 (KAI2) was first identified as a receptor of karrikins, smoke-derived germination stimulants. KAI2 is also considered a receptor of an unidentified endogenous molecule called the KAI2 ligand. Upon KAI2 activation, signals are transmitted through the degradation of D53/SMXL proteins via MAX2-dependent ubiquitination. Although components in the KAI2-dependent signaling pathway, namely MpKAI2A and MpKAI2B, MpMAX2, and MpSMXL, exist in the genome of the liverwort Marchantia polymorpha, their functions remain unknown. Here, we show that early thallus growth is retarded and gemma dormancy in the dark is suppressed in Mpkai2a and Mpmax2 loss-of-function mutants. These defects are counteracted in Mpkai2a Mpsmxl and Mpmax2 Mpsmxl double mutants indicating that MpKAI2A, MpMAX2, and MpSMXL act in the same genetic pathway. Introduction of MpSMXLd53, in which a domain required for degradation is mutated, into wild-type plants mimicks Mpkai2a and Mpmax2 plants. In addition, the detection of citrine fluorescence in Nicotiana benthamiana cells transiently expressing a SMXL-Citrine fusion protein requires treatment with MG132, a proteasome inhibitor. These findings imply that MpSMXL is subjected to degradation, and that the degradation of MpSMXL is crucial for MpKAI2A-dependent signaling in M. polymorpha. Therefore, we claim that the basic mechanisms in the KAI2-dependent signaling pathway are conserved in M. polymorpha.

Seed biopriming with cyanobacterial extracts as an eco-friendly strategy to control damping off caused by Pythium ultimum in seedbeds

This work highlights the ability of various cyanobacterial extracts from Anabaena spp., Tolypothrix spp., Nostoc or Trichormus, among others genera, to control the incidence of damping-off caused by Pythium ultimum in cucumber seedlings. Protocols applied aimed at the preliminary characterization of the cyanobacterial collection were very useful for predicting their phytotoxic, phytostimulating and biopesticidal capacity. First, the phytostimulatory or phytotoxic potential of a collection of 31 sonicated cyanobacterial extracts was analyzed by calculating the germination index in watercress seeds and the increase or loss of seedling weight. Likewise, the collection was characterized according to its ability to inhibit the growth of P. ultimum by dual culture bioassays and detached-leaf test. Finally, after selecting the most effective extracts, a preventive damping-off bioassay was performed based on cucumber seed biopriming. The strain SAB-M465 showed to be the most efficient strain against the in vitro growth of P. ultimum, while SAB-B912 was more discreet in this regard, but proved to be the most effective as a germination stimulator. Seed biopriming strategy with sonicated extracts of cyanobacteria revealed a remarkable promoter effect in the early stages of plant development, although only SAB-M465 was positioned as an effective control agent against damping-off caused by P. ultimum in cucumber seedbeds.

Moments of weaknesses - exploiting vulnerabilities between germination and encystment in the Phytomyxea.

Attempts at management of diseases caused by protozoan plant parasitic Phytomyxea have often been ineffective. The dormant life stage is characterised by long-lived highly robust resting spores that are largely impervious to chemical treatment and environmental stress. This review explores some life stage weaknesses and highlights possible control measures associated with resting spore germination and zoospore taxis. With phytomyxid pathogens of agricultural importance, zoospore release from resting spores is stimulated by plant root exudates. On germination, the zoospores are attracted to host roots by chemoattractant components of root exudates. Both the relatively metabolically inactive resting spore and motile zoospore need to sense the chemical environment to determine the suitability of these germination stimulants or attractants respectively, before they can initiate an appropriate response. Blocking such sensing could inhibit resting spore germination or zoospore taxis. Conversely, the short life span and the vulnerability of zoospores to the environment require them to infect their host within a few hours after release. Identifying a mechanism or conditions that could synchronise resting spore germination in the absence of host plants could lead to diminished pathogen populations in the field.

Biodegradable and Biocompatible Silatrane Polymers.

In this study, new biodegradable and biocompatible amphiphilic polymers were obtained by modifying the peripheral hydroxyl groups of branched polyethers and polyesters with organosilicon substituents. The structures of the synthesized polymers were confirmed by NMR and GPC. Organosilicon moieties of the polymers were formed by silatranes and trimethylsilyl blocks and displayed hydrophilic and hydrophobic properties, respectively. The effect of the ratio of hydrophilic to hydrophobic organosilicon structures on the surface activity and biological activity of macromolecules was studied, together with the effect on these activities of the macromolecules’ molecular weight and chemical structure. In particular, the critical micelle concentrations were determined, the effect of the structure of the polymers on their wetting with aqueous solutions on glass and parafilm was described, and the aggregation stability of emulsions was studied. Finally, the effect of the polymer structures on their antifungal activity and seed germination stimulation was examined.

Application of Markers Assisted Selection for Striga Hermonthica Resistance on Sorghum (Sorghum bicolor (L.) Moench

Sorghum (Sorghum bicolor L. [Moench]) is a staple food crop for smallholder farmers in arid and semi-arid(ASALs) regions worldwide, feeding over 500 million of the world's most resource-poor. Development of Striga Hermonthica resistant cultivars by conventional breeding is slow and have been hampered by the lack of efficient and reliable screening techniques in breeding programs. Molecular markers that are linked to witchweed resistance can expedite the development of resistant cultivars through the adoption of appropriate marker-assisted selection (MAS) strategies. Marker-assisted selection involves the selection of genotypes carrying a desirable gene(s) via linked markers; through MAS more rapid transfer of traits from donor parents to more elite locally adapted crop cultivars is possible with simple-sequence repeat (SSR) markers which have been initially used to detect polymorphism between the parent cultivars. Although costly to develop relative to some other classes of genetic markers, once developed, analysis by SSR markers is both easy and inexpensive. The highly polymorphic nature (high information content) and other favorable characteristics make them excellent genetic markers for a number of studies including marker-assisted selection and fingerprinting of germplasm collections. In this review, we summarize the molecular markers that are linked to the inheritance trait or low germination stimulant production is one of the recognized mechanisms of witch weed resistance.

Allelopathic Effect of Lamiaceae Plant Extracts on Seed Germination and Seedling Related Traits of Two linseed (Linum usitatissimum L.) Varieties

      A laboratory experiment was conducted during the 2017- 2018 growing season under laboratory conditions in the Biotechnology Laboratory/ Department of Biotechnology and Crop Science / College of Agricultural Engineering Sciences / University of Sulaimani/ Iraq. This factorial experiment conducted in a Randomized Complete Design with three replications. The first factor was two tested Linseed varieties (Local and Poland). The Second factor was aboveground biomass of three Lamiaceae plant species Spearmint (Mentha spicata), Peppermint (Mentha piperita) and Basil (Ocimum basilicum) The third factor was two kinds of dried (Spearmint, Peppermint and Basil) plant extracts methods (Aqueous and Alcoholic extraction). The fourth factor was three concentrations (1:1, 1:2 and 1:3) with control. Various germination attribute characters Germination Percentage (G%), Inhibition (-) /Stimulation (+) of Germination, Mean Germination Time (MGT), %, Coefficient Velocity of Germination (CVG), Germination Rate Index (GRI), Radicle Length (cm), Plumule Length (cm), Radicle Dry Weight (g), Plumule Dry Weight (g) were studied. From the results it was observed that Poland variety super passed Local variety in most of germination attribute characters, plant source Ocimum basilicum however had a higher allelopathic effect on inhibition of G% which lead to longer day of germination but gave higher plumule length and dry weight. Alcoholic extraction methods had a great effect on inhibition of G% which lead to longer germination time but gave higher CVG and plumule dry weight. (1:1) concentration gave higher inhibition of G% which leaded to longer days for germination while control gave maximum value for the rest of germination attribute characters. The obtained results from the interactions between all factors were various the most effective character was inhibition of G% which gave maximum values for each interactions between Poland variety and Ocimum basilicum, local variety and alcoholic extraction, Poland variety and (1:1) concentration, Ocimum basilicum and alcoholic extraction methods, Ocimum basilicum and (1:1) concentration, alcoholic extraction methods and (1:1) concentration, Local variety, Ocimum basilicum and alcoholic extraction methods, Local variety, Ocimum basilicum and (1:1) concentration, Ocimum basilicum, aqueous extraction and (1:1) concentration and Poland variety, Mentha piperita, alcoholic extraction and(1:1) concentration.

New pre-attachment Striga resistant sorghum adapted to African agro-ecologies.

Pre-attachment resistance to the parasitic plants Striga hermonthica and S. asiatica occurs in sorghum mutants designated low germination stimulant 1 (lgs1). However, only a few of these mutants have been identified and their resistance validated. Additionally, pre-attachment resistance in sorghum beyond lgs1 mutants has not been explored. We used lgs1-specific markers to identify new lgs1-like mutants in a diverse global sorghum collection. The sorghum collection was also evaluated for pre-attachment resistance against Striga using an in vitro assay that measured Striga germination activity and radicle growth. From a total of 177 sorghum accessions, 60 recorded mean germination levels of below 42%, which is comparable with the previously identified lgs1-like sorghum (SRN39 and IS9830) used as controls in this study. Furthermore, 32 of these accessions recorded Striga radicle lengths comparable or lower than the controls (0.42 mm). Thirty-eight accessions contained the lgs1 mutation and although overall, lgs1 mutants had considerably reduced Striga germination, some low inducers of Striga germination were wild-type for lgs1. Germination was positively but weakly correlated with radicle length pointing to additional radicle growth inhibitory activity. lgs1 mutations, alongside other mechanisms for low Striga germination stimulation, are prevalent in sorghum, and poor Striga radicle growth is suggestive of host-derived inhibition. As an outcome, our study makes available multiple Striga-resistant sorghum with adaptability to diverse agro-ecological regions in sub-Saharan Africa making immediate deployment possible. © 2021 Society of Chemical Industry.

The mechanism of host-induced germination in root parasitic plants.

Chemical signals known as strigolactones (SLs) were discovered more than 50 years ago as host-derived germination stimulants of parasitic plants in the Orobanchaceae. Strigolactone-responsive germination is an essential adaptation of obligate parasites in this family, which depend upon a host for survival. Several species of obligate parasites, including witchweeds (Striga, Alectra spp.) and broomrapes (Orobanche, Phelipanche spp.), are highly destructive agricultural weeds that pose a significant threat to global food security. Understanding how parasites sense SLs and other host-derived stimulants will catalyze the development of innovative chemical and biological control methods. This review synthesizes the recent discoveries of strigolactone receptors in parasitic Orobanchaceae, their signaling mechanism, and key steps in their evolution.

Field Screening of Sorghum [Sorghum bicolor (L.) Moench] Inbred Lines for Resistance to Striga [Striga hermonthica (Del.)] in East Hararghe, Ethiopia

Drought and the obligate Striga root parasite are two of the most important constraints to sorghum production in the northern and north eastern parts of Ethiopia. This study assessed the field reaction of selected advanced lines under Striga hermonthica infestation condition. Twenty-two sorghum genotypes with two resistant checks (Gobiye and SRN-39) and one susceptible check (Teshale) were evaluated under Striga hot spot area at Fadis Agricultural Research Center, Boko research station (Ethiopia) in the 2016 season using a 5x5 triple lattice design. During the field experiment, 11 parameters were measured among which Striga count emerged on each genotype was the indicator of resistance genotypes. Genotypes differed significantly in all measured parameters in their reactions to Striga. Genotypes 2006 MW 6044, ETSC 300003, ETSC 300081, 05 MW 6019, and ETSC 300080 showed Striga resistance in the field. Thus, these genotypes were the most promising sources of resistance to S. hermonthica. From field experiments, it could be suggested that genetic variability for resistance and tolerance is available in a range of genotypes, which could be used for future breeding and production in Striga infested areas. Keywords: genotypes, germination stimulant, parasitic weed, resistant, screening techniques DOI: 10.7176/FSQM/105-01 Publication date: February 28 th 2021

Using the Extract of “Saprolin” for Enhancing Grain Crops

The solution to the problems of enhancing the yield of grain crops in current agriculture development is not conceivable without applying new land processing technology and using mineral fertilizers. Simultaneously, to obtain environmentally friendly products in organic farming, it is required to use mineral organic fertilizers in large quantities. For these purposes, we developed manufacturing technology and proposed the extract of “Saprolin.” This extract, obtained from organogenic lake storage, is supposed to be used for pre-sowing seed treatment, contributing to providing the planting material with biologically active substances, macro- and microelements, and natural soil conservation. During the production of the extract from the obtained source material, solutions of different concentrations were prepared, and their features (viscosity, density, electrical conductivity, refractive index, and pH of the medium) were studied. Using the wheat seeds “Novosibirskaya-31” and barley “Astana-2000” as an example, under the influence of solutions of the obtained extract, the germination and vigor of seeds were determined. As it was proved in experiments, the obtained extract is a good germination stimulant for grain crop seeds. It has a greater effect on seed vigor. Nevertheless, seeds with high vigor are the most viable, give good yield, and provide high-quality products. The germination rate significantly affects plants’ survival, shows the speed of their growth, and characterizes the ability of seeds to germinate in the field evenly (good uniformity). The research determines the germination and vigor of seeds using processing solutions of different concentrations. The research shows that the obtained extract of “Saprolin” increases the germination of seeds of grain crops by 1%–9% and vigor by 12%–19%. The extract of “Saprolin” is classified as a herbal stimulant containing a water-soluble natural mineral substance for improving plant nutrition.

Identification and characterization of sorgomol synthase in sorghum strigolactone biosynthesis.

Strigolactones (SLs), first identified as germination stimulants for root parasitic weeds, act as endogenous phytohormones regulating shoot branching and as root-derived signal molecules mediating symbiotic communications in the rhizosphere. Canonical SLs typically have an ABCD ring system and can be classified into orobanchol- and strigol-type based on the C-ring stereochemistry. Their simplest structures are 4-deoxyorobanchol (4DO) and 5-deoxystrigol (5DS), respectively. Diverse canonical SLs are chemically modified with one or more hydroxy or acetoxy groups introduced into the A- and/or B-ring of these simplest structures, but the biochemical mechanisms behind this structural diversity remain largely unexplored. Sorgomol in sorghum (Sorghum bicolor [L.] Moench) is a strigol-type SL with a hydroxy group at C-9 of 5DS. In this study, we characterized sorgomol synthase. Microsomal fractions prepared from a high-sorgomol-producing cultivar of sorghum, Sudax, were shown to convert 5DS to sorgomol. A comparative transcriptome analysis identified SbCYP728B subfamily as candidate genes encoding sorgomol synthase. Recombinant SbCYP728B35 catalyzed the conversion of 5DS to sorgomol in vitro. Substrate specificity revealed that the C-8bS configuration in the C-ring of 5DS stereoisomers was essential for this reaction. The overexpression of SbCYP728B35 in Lotus japonicus hairy roots, which produce 5DS as an endogenous SL, also resulted in the conversion of 5DS to sorgomol. Furthermore, SbCYP728B35 expression was not detected in nonsorgomol-producing cultivar, Abu70, suggesting that this gene is responsible for sorgomol production in sorghum. Identification of the mechanism modifying parental 5DS of strigol-type SLs provides insights on how plants biosynthesize diverse SLs.

Razlike u alelopatskom učinku ekstrakata korijena pajasena i izoliranog ailantona na test-biljne vrste

The objective of this paper was to analyse allelopathic effect of 3 different aqueous solutions from tree of heaven’s (Ailanthus altissima (Mill.) Swingle) root extract and their dilutions on common wheat (Triticum aestivum L.), pigweed (Amaranthus retroflexus L.) and red bristlegrass (Setaria pumila L.). Investigated aqueous solutions obtained from tree of heaven’s root extract were root aqueous solution, aqueous solution with isolated ailanthone and root aqueous solution without ailanthone. Each of these solutions was diluted with water in ratio 1:4 and 1:16 before application on seeds of 3 test-plant species. Concentrated root and ailanthone aqueous solution and dilutions were equivalent to concentration of 0.48 mg/mL ailanthone. High allelopathic effect on radicle and shoot length of all test-species was proven for all investigated aqueous solution and their dilutions. Inhibitory effect on initial growth of all test-species was proportional to the increase in concentration in all treatments. The effect on initial growth was stronger in relation to the effect on germination of test-species. Lower concentrations caused stimulation of red bristlegrass germination. Pigweed was the most sensitive and common wheat the least sensitive test-species. The least significant effect was measured in the aqueous solution of isolated ailanthone.

Synthesis of Analogs of Strigolactones and Evaluation of Their Stability in Solution.

Strigolactones (SLs) are new plant hormones that play an important role in the control development of plants. They are germination stimulants for seed of parasitic weeds, are the branching factor of arbuscular mycorrhizal fungi and inhibitors for bud outgrowth and shoot branching. Natural SLs contain an annulated system of three rings (ABC scaffold) connected to a furanone (the D-ring) by an enol ether unit. The natural distribution of strigolactones is low, and their synthesis is long and difficult. Therefore, SL analogs are designed to have the same bioactiphore as natural SLs and an appreciable bioactivity. For the design a model is used based on the natural bioactiphore. Typical SL analogs are GR24, Nijmegen-1, and EM1 (derived from ethyl 2-phenylacetate). The synthesis of these SL analogs is reported together with their stability in aqueous solution.

Positively Charged Water as a Plant Germination Stimulator

There is a powerful electromagnetic force on Earth that moves positive charges up and negative charges down. This force participates in many phenomena occurring both in the Earth's atmosphere and on its surface, both in the grandiose and in the almost imperceptible. So, this force causes atmospheric discharges, both ascending currents of positive charges, namely, heavenly spirits and elves, and descending currents of negative charges, namely ordinary lightning. In addition, this force distributes the water within the clouds, directing the positively charged vapor upward and the negatively charged downward. Also, this force determines the ability of salts dissolved in positively charged water to form ascending plant-like crystals. All this made it possible to assume the participation of the same force in the distribution of water inside plants. In particular, it was assumed that under the action of this force, positively charged water moves upward inside the plants, and negatively charged water moves downward. Accordingly, it was assumed that positively charged water stimulates plant growth better than negatively charged water. Both of these assumptions have been verified experimentally. As expected, positively charged water stimulated the germination of various plants, while negatively charged water suppressed it. All this made it possible to propose a fundamentally new mechanism for the distribution of water inside vertical plants. In addition, other mechanisms were analyzed to explain the opposite effects of positively and negatively charged water on germinating plants.

Adaptation of the parasitic plant lifecycle: germination is controlled by essential host signaling molecules.

Parasitic plants are plants that connect with a haustorium to the vasculature of another, host, plant from which they absorb water, assimilates, and nutrients. Because of this parasitic lifestyle, parasitic plants need to coordinate their lifecycle with that of their host. Parasitic plants have evolved a number of host detection/host response mechanisms of which the germination in response to chemical host signals in one of the major families of parasitic plants, the Orobanchaceae, is a striking example. In this update review, we discuss these germination stimulants. We review the different compound classes that function as germination stimulants, how they are produced, and in which host plants. We discuss why they are reliable signals, how parasitic plants have evolved mechanisms that detect and respond to them, and whether they play a role in host specificity. The advances in the knowledge underlying this signaling relationship between host and parasitic plant have greatly improved our understanding of the evolution of plant parasitism and are facilitating the development of more effective control measures in cases where these parasitic plants have developed into weeds.

Triflumizole as a Novel Lead Compound for Strigolactone Biosynthesis Inhibitor

Strigolactones (SLs) are carotenoid-derived plant hormones involved in the development of various plants. SLs also stimulate seed germination of the root parasitic plants, Striga spp. and Orobanche spp., which reduce crop yield. Therefore, regulating SL biosynthesis may lessen the damage of root parasitic plants. Biosynthetic inhibitors effectively control biological processes by targeted regulation of biologically active compounds. In addition, biosynthetic inhibitors regulate endogenous levels in developmental stage- and tissue-specific manners. To date, although some chemicals have been found as SL biosynthesis inhibitor, these are derived from only three lead chemicals. In this study, to find a novel lead chemical for SL biosynthesis inhibitor, 27 nitrogen-containing heterocyclic derivatives were screened for inhibition of SL biosynthesis. Triflumizole most effectively reduced the levels of rice SL, 4-deoxyorobanchol (4DO), in root exudates. In addition, triflumizole inhibited endogenous 4DO biosynthesis in rice roots by inhibiting the enzymatic activity of Os900, a rice enzyme that converts the SL intermediate carlactone to 4DO. A Striga germination assay revealed that triflumizole-treated rice displayed a reduced level of germination stimulation for Striga. These results identify triflumizole as a novel lead compound for inhibition of SL biosynthesis.

Growth-Stimulating Activity of Natural Polymer-Based Nanocomposites of Selenium during the Germination of Cultivated Plant Seeds.

The growth-stimulating activity of three selenium nanocomposites (NCs) in various matrices based on arabinogalactan (NC Se/AG, 6.4% Se), starch (NC Se/St, 2% Se), and carrageenan (NC Se/Car, 12% Se) with respect to plants of radish, soybean, and potato was investigated. It was shown that the treatment of plant seeds with NCs stimulated root growth during germination. It was found that the studied NCs affected both the level of lipid peroxidation and the activity of the antioxidant enzyme glutathione peroxidase (GPX). The treatment of radish seeds with NCs stimulated root growth during their germination and reduced the content of diene conjugates (DC) in root tissues. It was shown that soaking seeds in NC Se/AG solution increased the GPX activity in the tissues of the radish root by 40%. Stimulation of soybean root growth under the influence of NC Se/Car may also be associated with the activation of GPX. Furthermore, in potato plants, this NC led to the stimulation of germination; however, this was probably due to the activation of other antioxidant enzymes. The results obtained allow us to consider Se NCs as potential plant growth stimulants.

On improving strigolactone mimics for induction of suicidal germination of the root parasitic plant Striga hermonthica.

Strigolactones (SLs) are plant hormones that regulate the branching of plants and seed germination stimulants of root parasitic plants. As root parasites are a great threat to agricultural production, the use of SL agonists could be anticipated to provide an efficient method for regulating root parasites as suicidal germination inducers. A series of phenoxyfuranone-type SL mimics, termed debranones, has been reported to show potent bioactivities, including reduction of the tiller number on rice, and stimulation of seed germination in the root parasite Striga hermonthica. To exert both activities, two substituents on the phenyl ring of the molecules were important but at least a substituent at the 2-position must be an electron-withdrawing group. However, little is known about the effect of the properties of the substituents at the 2-position on bioactivities. Here, we found that different substituents at the 2-position give different preferences for bioactivities. Halogenated debranones were more effective than the others and SL agonist GR24 for inhibiting rice tiller but far less effective in the induction of S. hermonthica germination. Meanwhile, nitrile and methyl derivatives clearly stimulated the germination of S. hermonthica seeds. Although their IC50 values were 100 times higher than that of GR24 in the receptor competitive binding assay, their physiological activities were approximately 1/10 of GR24. These differences could be due to their uptake in plants and/or their physicochemical stability under our experimental conditions. These findings could support the design of more potent and selective SL agonists that could contribute to solving big agricultural issues.

PURPLE BLOTCH AS SEEDBORNE DISEASE OF ONION AND ITS CONTROL*

Alternaria porri (Ellis). Ciffen causes serious problems in onion plants. It is transmitted with naturally infected seeds and bulbs from the previous crop to infect the new flower stalk of the next crop. Also, onion bulb infection decreases seed vitality. Infected sample were collected from four locations; Giza, Sharkia, Kalubia and Menofia governorates to measure disease severity. Experiments have been done on naturally infected seeds and bulbs from the previous year by cultivating infected bulbs. Infection was classified into five categories based on the percentage of disease severity. Pathogen transmission by the onion seed was investigated, either as internal or external infection type, Five different ratios of blub infection was determined as; 14.2%, 25.2%, 41.5%, 61.1%, 87.5%. Also, trials were carried out in the current study to evaluate the efficiency of some botanical, biological and chemical as seed dressing treatments for infected seeds before sowing it, i.e. (Achook, Bougainvillea sp., Bacillus sp., Trichoderma sp. and Score). Results showed that disease severity was increased in the blub produced from previous crop, the number of flower stalk was reduced, and its disease severity was increased. Seeds produced from infected blub showed reduction in germination percentage. The most effective seed dressing treatment on seed germination were obtained by using score & Achook treatment compared with other seed dressing treatments. All treatments including Score, Achook, Bacillus sp., Bougainvillea sp., and Trichoderma sp.; respectively showed variable seed stimulation of germination compared with plants in the control treatment.

Biostimulants in vegetable seeds submitted to germination and vigor tests

The use of bioproducts, economically viable, are of extreme importance in the protection and stimulation of germination in vegetable crops. This work evaluated the effect of the microorganisms Azospirillum brasiliense, Bacillus sub-tilis, Trichoderma harzianum and the commercial seed treatment product (Fipronil + Pilaclostrobin and Methyl Thiophanate) on seeds and seedlings of lettuce (Lactuca sativa), carrot (Daucus carota) and tomato (Solanum lycopersicum). The seeds were inoculated before being submitted to the germination test. The chemical treatment proved ineffective in protecting the seed of all crops and stimulating germination. T. harzianum increased the germination index of lettuce seeds, had better values in root system size in tomato crop and stimulated radicle emission in carrot. B. subtilis stood out in dry matter accumulation in tomato crop. The microorganisms B. subtilis and T. harzianum present potential for vegetable seed treatment.