Seed Germination Percentage Research Articles

Investigation of insect population density, species composition and associated losses in chickpea seeds stored on farms in Ethiopia

This survey aimed to investigate the extent of insect infestations, associated losses, and insect species abundance in farm-stored chickpea seeds across five chickpea growing districts in Ethiopia. Despite being the largest producer, consumer, and exporter of chickpea in Africa, insect pest infestations have caused significant losses to Ethiopia's chickpea industry. Results showed that Callosobruchus chinensis (L.) was the most prevalent insect species, followed by Sitophilus oryzae (L.) and Tribolium confusum (J. du Val). The insect pests infested both local and improved chickpea varieties, and traditional containers and polypropylene bags were used for storage. The percentage of insect-damaged seed ranged from 4.61% to 14.48%, while the seed weight loss ranging from 1.13% to 4.55%. The range of seed germination percentages was from 65% to 88%, with a mean rate of 71%. These losses significantly affect the market value of the crop as grain and its use as seed, affecting farmers' income and food security. Therefore, it is crucial to develop effective solutions to prevent the loss of farm-stored chickpea in Ethiopia.

Evaluation of cytogenetic effect of pesticides in onion root meristem

Farmers use chemical insecticides, fungicides and herbicides to control insect pests, diseases and weeds. Extensive use of chemical pesticides is becoming hazardous to the environment and human and animal health. Biopesticides have been used as a safer alternative. Cytogenetic analysis is needed to determine the effects of chemical and biopesticides in plants if these are used frequently in an improper concentration. This investigation assessed the effects of a chemical systemic insecticide ‘Actara 25WG’ and a bioinsecticide ‘Bio Sona’ for seed germination and on root mitosis of five onion varieties. Three concentrations of Actara 25WG were used as seed treatment, viz., 25 g, 50g (recommended) and 75 g/kg seed. Similarly, Bio Sona was applied @ 2 %, 5 % (recommended) and 8 % concentrations. Depressing effects of the two pesticides were recorded in seed germination percentage except for Bio Sona 2% in variety Bhima Shakti. Actara 25WG and Bio Sona showed either mitoenhancing or cytotoxic effects depending on dose and variety. These pesticides also induced genotoxicity at recommended and higher doses on root tip cells as indicated by the frequency of total chromosomal aberrations like binucleate cells, clumps, stickiness, disturbed stage, laggards, bridges and fragments. Bio Sona at higher concentrations showed more aberrations than Actara 25WG. There were differential responses of the varieties to the pesticide treatment. More research must be done on the effects of chemicals and biopesticides in plants to assess their cytogenotoxicity.

The Development of Germination Tests and Breaking Dormancy Techniques of Bitter Seeds (Andrographis paniculata (Burm. f.) Wall. ex Nees)

Bitter seed (Andrographis paniculata (Burm. f.) Wall. ex Nees) is a medicinal species used in phyto pharmacy and known as ‘the king of bitterness’. Bitter seeds contain andrographolide,
 a diterpenoid lactone which is widely used in Indian medicine for snake bites and for the treatment of hepatitis. The research was conducted at the Seed Management Unit experimental field of the Indonesian Spice and Medicinal Plants Research Institute (IMACRI) and at the Seed Quality Testing Laboratory of IPB University, Indonesia, from January until May 2021. The objectives of the study is to determine the best germination test and effective breaking dormancy techniques to increase the germination percentage of bitter seeds. The study was arranged in a completely randomized design with two-factors. The first factor was seed treatments, i.e., control/untreated, chemical scarification with 5% H2SO4 for 10 minutes, and 5% H2SO4 followed by a 100 ppm GA3 soaking treatment. The second factor conducted was germination test methods according to International Seed Testing Association, i.e., germinating the seeds on the top of paper and in pleated paper. Our study demonstrated that seed treatment with H2SO4 can break seed dormancy and speed up seed germination. Bitter seed germination on the pleated paper was higher than with the top-of-paper method. Bitter seed germination with the top-of-paper method was at 65 DAS and the final germination was at 80 days after sowing (DAS), and the final germination was 55.2%. With the pleated paper method, the seeds germinated at 30 DAS and the final germination was at 70 DAS, and the final the germination of 59.2%.

Efficacy of ethanolic and aqueous extract of some plants on some biological aspects of cowpea beetle Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) – A comparative study

The present study included to know about the effectiveness of steep chickpea Cicer arietinum L. seeds with ethanolic and aqueous extracts of the Oleander Nerium oleander, Basil Ocimum basilicum, Chinaberry Melia azedarach, and Natgrass Cyperus rotundus leaves with four concentration (0.5, 1.0, 2.5, 5.0) % on some biological sides of the cowpea beetle Callosobruchus maculatus (Fab.). The present study showed the superiority of leaves extracts of Oleander and Chinaberry as compared with leaves extracts of Basil and Natgrass, as well as the superiority of aqueous extracts as compared with ethanolic extracts of the present study plant's leaves on some biological sides of the cowpea beetle C. maculatus (Fab.). Also, the present study showed that the least number of laid eggs was recorded at a concentration of 1.0% of the ethanolic extract of the chinaberry with several 31.5 eggs, the least percentage of hatched eggs was recorded in the aqueous extract of chinaberry at a concentration of 5.0% with a percentage of 64.8%, the highest larval stage period was recorded at a concentration of 5.0% of the ethanolic extract of the chinaberry with a period of 19.8 days. Total mortality (100%) in the larval stage was achieved with a concentration of 5.0% of the aqueous extracts of the Oleander and Chinaberry. The highest pupal stage period was recorded at 5.0% of the ethanolic extract of the chinaberry at 14.8 days. The highest mortality in the pupal stage was recorded at a concentration of 2.5 % of the aqueous extract of the chinaberry with a percentage of 23.0%. The least number of emerged adults in the first generation and least percentage of productivity were recorded at a concentration of 5.0 % of the aqueous extracts of the Oleander and Chinaberry, with several 0.0 adults and a percentage of 0.0%, respectively.The study also showed that the germination percentage of Chickpea seeds was not significantly affected when it was treated with different concentrations of ethanolic and aqueous extracts of the present study plants' leaves.

Germination and Secondary Dormancy Of Hydrocharis Dubia (Blume) Seed

The germination of freshly harvested, dry-stored and wet-stored Hydrocharis dubia seeds were treated with exogenous GA3, cold stratification, puncture, different temperatures and combined treatments under controlled laboratory conditions. The results showed that: The optimal temperature for H. dubia seed germination is 23℃ and fresh seed has conditional dormancy, which can be released by puncture. Seed germination percentage decreased significantly after dry seed storage and the main reason for the reduction of "seed germination ability" by dry storage was that dry storage. caused secondary dormancy but a reduction of seed vigor. The secondary dormancy is non- deep physiological dormancy, which can be released by cold stratification, seed puncture and combination treatments. The findings provided further insight into how germination strategy contributes to the seed bank formation and species reproduction. Bangladesh J. Bot. 52(2): 429-435, 2023 (June)

Effect of gibberellic acid on germination and seedling growth of Soybean (Glycine max L. Merrill)

The germination percentage of Soybeans is susceptible and affected by many external and internal factors. Screening of three varieties of soybeans (Shimaa, Industrial2, and M103) during laboratory germination and field emergence was conducted to identify varieties with superior performance under four concentrations of gibberellic acid (0, 75, 150, 225 ppm). Experiments were conducted in Field Crops Dept./College of Agriculture/Tikrit University. Results showed significant differences between gibberellic acid concentrations, varieties, and interactions for all studied traits. Seeds treated with a concentration of (150 ppm) were superior in laboratory germination and field emergence percentages, averaging 92.58% and 59.08%, respectively. Varieties of Shimaa and M103 were superior in giving the highest germination percentage in the laboratory, 87.5% and 87.94%, respectively. The Shimaa variety outperformed, giving the highest field emergence percentage, 58.00%. Interaction of Shaima x150 ppm (GA3) showed the highest laboratory germination percentage and highest field emergence percentage, 96.75% and 63.25%, respectively. These Results indicate that the use of gibberellic acid significantly increased the germination rate of soybean seeds and field emergence rate due to the role of this hormone in stimulating the synthesis of enzymes necessary for germination and seedling vigor. Keywords: Soybean; GA3; Seed germination percentage; Field emergence percentage.

Effect of Arbuscular Mycorrhizal Fungi on Growth and Development of Zea Mays L.

In the present study, effect of Arbuscular Mycorrhizal Fungi (AMF) on seed germination, nutrient uptake, plant growth, root colonization and chlorophyll content of the Zea mays L. was evaluated. The soil samples collected from maize field (Morgaon, Pune, Maharashtra) were used for the isolation of AMF spores by using wet sieving and decanting method. The International Culture Collection of (Vesicular) Arbuscular Mycorrhizal Fungi (INVAM) worksheet was used for diagnosing the spores. The completely randomized design with three replicates was used for pot culture experiment. The chlorophyll (a, b and total chlorophyll) content from the leaves of the maize plant was successfully determined by Arnon method. Three AMF spores (Gigaspora sp., Acoulospora sp. and Glomus mosseae) were identified and selected for further study. The application of AMF inoculum significantly increases the overall seed germination percent in saline soil (50% and 25% in nonsterile and sterile saline soil) and root and shoot length (13.68 cm and 10.02 cm increase in nonsterile normal soil while 8.93 cm and 11cm increase in nonsterile saline soil) when compared with control. Highest increase in pigment was found in sterile soil inoculated with AMF [chlorophyll a (47.80%), b (47.76) and total chlorophyll (45.56%)]. In root colonization study, different typical AMF structures (such as vesicles, spores, intra and extra radical hyphae) inside the roots of the maize plant were evaluated by microscopic observations. Finally, the nutrient profiling of the soils from all pots showed increased N, P, K, Ca, Fe, Mn, Cu and Zn when compared with control.

Effects of OsAOX1a Deficiency on Mitochondrial Metabolism at Critical Node of Seed Viability in Rice.

Mitochondrial alternative oxidase 1a (AOX1a) plays an extremely important role in the critical node of seed viability during storage. However, the regulatory mechanism is still poorly understood. The aim of this study was to identify the regulatory mechanisms by comparing OsAOX1a-RNAi and wild-type (WT) rice seed during artificial aging treatment. Weight gain and time for the seed germination percentage decreased to 50% (P50) in OsAOX1a-RNAi rice seed, indicating possible impairment in seed development and storability. Compared to WT seeds at 100%, 90%, 80%, and 70% germination, the NADH- and succinate-dependent O2 consumption, the activity of mitochondrial malate dehydrogenase, and ATP contents all decreased in the OsAOX1a-RNAi seeds, indicating that mitochondrial status in the OsAOX1a-RNAi seeds after imbibition was weaker than in the WT seeds. In addition, the reduction in the abundance of Complex I subunits showed that the capacity of the mitochondrial electron transfer chain was significantly inhibited in the OsAOX1a-RNAi seeds at the critical node of seed viability. The results indicate that ATP production was impaired in the OsAOX1a-RNAi seeds during aging. Therefore, we conclude that mitochondrial metabolism and alternative pathways were severely inhibited in the OsAOX1a-RNAi seeds at critical node of viability, which could accelerate the collapse of seed viability. The precise regulatory mechanism of the alternative pathway at the critical node of viability needs to be further analyzed. This finding might provide the basis for developing monitoring and warning indicators when seed viability declines to the critical node during storage.

Determination of the best culture medium for establishment, propagation and conservation in vitro of citrus rootstocks

In micropropagation and in vitro conservation various nutrient media have been used for regeneration of citrus rootstocks. In this respect, the present study was conducted to define, among three culture media, one of common use (MS) and two developed for arboreal species (RMAN and WPM), the best formulation for the establishment, propagation and conservation in vitro. Plantlets from 10 citrus rootstocks grown in the three media were micropropagated and conserved in vitro for 18 months, during which they were submitted to three subcultures at six-month intervals. The RMAN and WPM formulations provided the highest seed germination percentages, but the analysis of the morphogenetic response during the subcultures revealed that the composition of RMAN resulted in loss of the regenerative capacity of the explants in the second subculture. As occurred for RMAN, there was reduced development of the plants cultivated in MS with each new subculture. The plants grown in WPM, maintained or increased their regenerative capacity during the subcultures. Among the media studied, WPM was the most efficient for micropropagation and in vitro conservation, while the RMAN limited the in vitro germination of the rootstocks.

Analysis of MsTERT Gene Expression Profile in Alfalfa (Medicago sativa) Indicates Their Response to Abiotic Stress and Seed Aging.

Seed aging is always taken as a crucial factor for vigor loss due to delayed seed germination and seedling growth, which limits hay production. Many studies have found that telomeres are closely related to abiotic stress and seed vigor. However, the molecular mechanism of telomeres' response to abiotic stress, seed vigor, and the maintenance mechanism of plant telomere homeostasis still remain unclear. Alfalfa (Medicago sativa) enjoys the title of "King of Forage", and is an important protein forage for the dairy industry as planted in the world. This comprehensive investigation was performed to explore the molecular characterization, phylogenetic relationship, and gene expression analysis of MsTERT under abiotic stress and during seed aging in alfalfa. In this study, MsTERT was identified from the 'Zhongmu 1' alfalfa genome and encoded a coding sequence (CDS) of 3615 bp in length, consisting of telomerase- RNA-Binding Domain (RBD) and Reverse Transcriptase (RT) domains, 1024 amino acids, an isoelectric point of 9.58, and a relative molecular mass of 138.94 kD. Subcellular localization showed that MsTERT was mainly localized in the nucleus and mitochondria. The results of the expression profile showed that MsTERT was observed to respond to various stress conditions such as salt (100 mmol/L NaCl) and drought (20% PEG 6000). Furthermore, exogenous hormones IAA, ABA, and GA3 showed the potential to affect MsTERT expression. Additionally, MsTERT also responded to seed aging. Our results revealed a marginal but significant association between relative telomere length, MsTERT expression, and seed germination percentage, suggesting that the length of telomeres was shortened, and expression of MsTERT decreased with alfalfa seed aged. These results provide some evidence for the hypothesis of relative telomere length and/or TERT expression serving as biomarkers of seed aging. Although this finding is helpful to offer a new way to elucidate the molecular mechanism of vigor loss in alfalfa seed, further investigation is required to elucidate the molecular mechanism by which the MsTERT gene regulates seed vigor.

Germination ecology and growth phenology of cowvine (Ipomoea lonchophylla) as influenced by environmental parameters

AbstractCowvine (Ipomoea lonchophylla J.M. Black) is a native and widely spread summer broadleaf weed in Australia. It contains glycoresins, which are toxic to livestock. However, limited information is available on seed germination ecology and growth phenology of this species. A series of experiments were conducted to determine the response of I. lonchophylla to different environmental conditions. Results showed that the primary dormancy exhibited by I. lonchophylla is due to the physical impediment of the hard seed coat. The seed germination percentage was the highest at the constant temperature of 27 C and alternating temperatures of 35/25 C. Germination of I. lonchophylla was not stimulated by light, suggesting that this species is non-photoblastic. Ipomoea lonchophylla germination was intolerant of a medium to high level of salt stress, and germination was completely inhibited at 250 mM NaCl. The emergence of I. lonchophylla was not restricted by seeding depth up to 8 cm, but only 5% emergence was recorded when seeds were planted at a 16-cm depth. The germination percentage was also drastically reduced by 90% to 100% after exposure to either 3 mo in silage, 48-h digestion in steers, or silage plus digestion treatments. The growth and reproductive phenology of I. lonchophylla was affected by emergence time. Plants that emerged in late spring (November 15) were able to produce more berries per plant than those that emerged in midsummer (January 15) in southern New South Wales. Information gained in our study concerning high soil salinity, ensiling, and digestion will help to develop more sustainable and effective integrated weed management strategies for controlling and reducing the spread of this weed.

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions.

Seed vigor is an important aspect of seed quality. High-vigor seeds show rapid and uniform germination and emerge well, especially under adverse environmental conditions. Here, we determined hydrotime model parameters by incubating seeds at different water potentials (0.0, -0.2, -0.4, -0.6, and -0.8 MPa) in the laboratory, for 12 seed lots of Chinese milk vetch (Astragalus sinicus) (CMV), a globally important legume used as forage, green manure, and a rotation crop. Pot experiments were conducted to investigate the seedling emergence performance of 12 CMV seed lots under control, water stress, salinity stress, deep sowing, and cold stress conditions. Meanwhile, the field emergence performance was evaluated on two sowing dates in June and October 2022. Correlation and regression analyses were implemented to explore the relationships between hydrotime model parameters and seedling emergence performance under various environmental conditions. The seed germination percentage did not differ significantly between seed lots when seeds were incubated at 0.0 MPa, whereas it did differ significantly between seed lots at water potentials of -0.2, -0.4, and -0.6 MPa. The emergence percentage, seedling dry weight, and simplified vigor index also differed significantly between the 12 seed lots under various environmental conditions. Ψb(50) showed a significant correlation with germination and emergence performance under various environmental conditions; however, little correlation was observed between θH or σφb and germination and emergence. These results indicate that Ψb(50) can be used to estimate seed vigor and predict seedling emergence performance under diverse environmental conditions for CMV and similar forage legumes. This study will enable seed researchers, plant breeders, and government program directors to target higher seed vigor more effectively for forage legumes.

A Rapid and Reliable Method for Evaluating Cotton Resistance to Fusarium Wilt Race 4 Based on Taproot Rot at the Seed Germination Stage.

Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) is a soilborne fungal pathogen threatening U.S. cotton production. The objective of this study was to develop a reliable and efficient method to evaluate cotton for FOV4 resistance based on taproot rot during seed germination through five growth chamber tests and two greenhouse tests. Seeds from eight cotton cultivars (Set 1) were germinated in a paper towel for 6 days, and taproots were inoculated with a FOV4 conidial suspension using three inoculation methods (i.e., taproot dipping, taproot wounding, and paper towel drenching), in addition to seed soaking before germination. The taproot rot-based disease incidence (DI) and disease severity rating (DSR), seed germination percentage (SGP), and plant fresh weight (PFW) were measured 7 days after inoculation. Taproot dipping was the most efficient and reliable evaluation method. The SGP and PFW were not significantly correlated with the DI and DSR, making them inappropriate to use in resistance evaluation. Pima DP 359 RF and PHY 881 RF were the most resistant with the lowest root rot. The taproot dipping method was repeated in another test and confirmed in two tests using another set of eight cultivars (Set 2). The taproot rot-based DSR at germination was significantly correlated with the DSR at the seedling stage in the greenhouse in both sets and with previous results in seedling mortality in the greenhouse and field in Set 2. The results suggest that the response to FOV4 infections at the seed germination stage is overall congruent with that at the seedling stage.

Germination and Early Seedling Growth of Three Arabica Coffee Varieties at Four Seed Soaking Water Temperatures

It takes three weeks to analyze the germination percentage of Arabica coffee seeds germinated using paper and a three-day water-soaking method. This 3- week period is considered too long for seed distribution to remote areas. Thus, we need to modify this germination test needs to be modified to have a shorter test time. Temperature treatment is one method to break the seed dormancy period for faster germination. This study aimed to obtain a faster and more precise method of germination test on Arabica coffee seeds. This study employed a 3 x 4 factorial complete randomized block design with three replications in which Arabica coffee varieties of Gayo-1, Kartika-1, and Sigararutang as the first factor and the temperature of the soaking water of the seeds (25 0C, 50 0C, 75 0C, and 90 0C) as the second factor. The research was conducted at the Plant Breeding Laboratory, Indonesian Coffee and Cocoa Research Institute (ICCRI), for four months. Findings showed that the interaction between treatments was only found in seed germination percentage. Soaking in room temperature water (control) for three days was better than using water at 50 0C, 75 0C, and 90 0C on the germination percentage, sprout length, and vigor index. Observations of Arabica seed germination could be shortened to 14 days compared to the standard 21-day observation. During the seedling phase, the shoot growth and root growth of the Arabica coffee seeds soaked in water at 50 0C showed no significant difference from the control. Soaking at 75 0C and 90 0C damaged the embryo and resulted in rotten seeds. Different varieties led to different germination percentages, sprout lengths, and fresh weight of seedling shoots. The method of soaking at 50 0C for 30 minutes could be used as an alternative in germination test of Arabica coffee seeds because it showed small negative effect on germinated seed and seedling quality but it shorten thetesting time for three days.

Effect of Saline Treatment on Seed Germination of Adzuki Beans

Salinity is one of the major causes of abiotic stress for plant development and growth worldwide, and affects crop yield significantly. To analyse the level of salinity tolerance among 'Ji Hong 20' (JH20), 'Jin Xiao Dou 5' (JXD5) and 'Jin Xiao Dou 6' (JXD6) adzuki bean cultivars, nine saline levels (0.0, 32.5, 65.0, 97.5, 130.0, 162.5, 195.0, 227.5 and 260.0 mmol L-1 NaCl) were applied during seed germination. Germination potentials and final germination percentages of all cultivars were significantly reduced by NaCl with the level at which germination was affected being dependent on cultivar. The JXD5 seeds had the highest germination index and vigour index at the same NaCl concentrations, followed by the JH20 and then JXD6 seeds. The shoot and root lengths of JH20 and JXD5 were significantly shorter at NaCl concentrations > 65.0 mmol L-1, while those of JXD6 were reduced at salinity > 32.5 mmol L-1. Quadratic regression models could well predict the germination percentage of JH20, JXD5 and JXD6 seeds under saline stress.

Non-deep physiological dormancy and germination characteristics of Primula florindae (Primulaceae), a rare alpine plant in the Hengduan Mountains of southwest China.

Timing of seed germination is directly related to the survival probability of seedlings. For alpine plants, autumn-dispersal seeds should not germinate immediately because the cold temperature is not conducive to the survival of seedlings. Seed dormancy is a characteristic of the seed that prevents it from germinating after dispersal. Primula florindae is an alpine perennial forb endemic to eastern Tibet, SW China. We hypothesized that primary dormancy and environmental factors prevent seeds of P. florindae to germinate in autumn and allow them to germinate at the first opportunity in spring. We determined how GA3, light, temperature, dry after-ripening (DAR) and cold-wet stratification (CS) treatments affect seed germination by conducting a series of laboratory experiments. Firstly, the effects of gibberellic acid (GA3; 0, 20, and 200 mg L-1) on germination of freshly shed seeds at alternating temperatures (15/5 and 25/15 °C) were immediately investigated to characterize seed with a physiological dormancy component. Then, the fresh seeds treated with 0, 3, and 6 months of after-ripening (DAR) and cold-wet stratification (CS) were incubated at seven constant (1, 5, 10, 15, 20, 25, and 30 °C) and two alternating temperatures (5/1, 15/5, and 25/15 °C) at light and dark conditions. Fresh seeds were dormant, which only germinated well (>60%) at 20, 25, and 25/15 °C in light but not at ≤15 °C and to higher percentages in light than in dark. GA3 increased germination percentage of fresh seeds, and DAR or CS treatments increased final germination percentage, germination rate (speed), and widened the temperature range for germination from high to low. Moreover, CS treatments reduced the light requirement for germination. Thus, after dormancy release, seeds germinated over a wide range of constant and alternating temperatures, regardless of light conditions. Our results demonstrated that P. florindae seeds have type 2 non-deep physiological dormancy. Timing of germination should be restricted to early spring, ensuring a sufficient length of the growing season for seedling recruitment. These dormancy/germination characteristics prevent seeds from germinating in autumn when temperatures are low but allow them to germinate after snowmelt in spring.

Utjecaj nadmorske visine i starosti zatvorenog češera na klijanje sjemena crvenog bora (Pinus brutia Ten.)

This study investigated changes in the germination rate and germination percentage of seeds obtained from closed pine cones (Pinus brutia Ten.) of different ages collected at different altitudes. The seeds used in the study were obtained from closed cones (3/control, 4, 5, 6, and 7 years old) in the lower (0-200 m) and higher (800-1000 m) altitude zones of the Mersin-Anamur region in a section from the sea to the interior. The seeds germinated in the dark at a constant temperature of 20 °C for 28 days. At the end of the germination test, a two-way analysis of variance was performed on the germination percentage data on the 10<sup>th</sup>, 14<sup>th</sup>, and 28<sup>th</sup> days, and the interactions between altitude and cone age were found to be significant (p = 0.05). As a result of the significant interaction between the two groups, the mean separation test (Tukey test) showed that the highest germination rate was 89.0% in the control group, and the lowest was 77.5% in the 7-year-old cones. In the higher altitude zone, the highest germination rate was 74.0% and the lowest was 71.0%, obtained from 5- and 7-year-old seeds, respectively. In general, germination values in the lower altitude zone were higher than those in the higher altitude zone. However, the decrease in germination values from the control to the 7-year-old seeds was greater in the lower altitude zone than in the higher altitude zone. The study found that altitude was more effective than cone age on the germination rate and percentage.

EFFECT OF GEOGRAPHIC LOCATION ON YIELD AND QUALITY OF SEED OF BJRI TOSSA PAT-8

The experiment was conducted in two locations, namely Manikganj and Noakhali to evaluate the effect of geographic position on the performance of seed yield and yield contributing characters of BJRI Tossa Pat-8. The result of data analysis revealed that the seed yield of BJRI Tossa Pat-8 in Manikganj (7.30g) was higher than the yield of Noakhali (5.63g). On the basis of morphology such as plant height, top diameter, base diameter, capsule length, number of seeds/pod, number of pods/plant & number of branches/plant, superior performance of Manikganj was observed to Noakhali. It was worth noticing that germination percentage of seeds of Manikganj (95%) was overwhelmingly higher than the seeds of Noakhali (84%). Geographic position also exerts significant effect on seed emergence and 1000 seed weight. Seeds harvested from Manikganj witnessed 90% of field emergence in contrast to only 72% in Noakhali. Similarly, a significant difference in 1000 seed weight was observed, 1000-seed weight of the seeds of Manikganj and Noakhali were 2.098g and 1.892g respectively.

Effects of chitosan nanoparticles coating on delaying of seed soybean (Gycine max) deterioration

High-quality seed is always demanded by farmers and may result in up to a 30% increase in crop yields. One of the major constraints ofsoybean cultivation is that soybean seeds quickly deteriorate in storage due to high lipid and protein content. Coating technology withvarious coating formulas is needed to extend seeds’ shelf life. This research aimed to determine the effects of CSNPs (chitosan-waxnanoparticles) and CS (chitosan-wax) as active coating ingredients in delaying the Deterioration of soybean seed quality. Two varietiesof soybean seed and three coating treatments (control, CS, and CSNPs) were used during storage for six months. Each treatment wasreplicated four times, and the observation was conducted monthly. The result showed that coating treatment could significantly suppressthe increase in water content and the decrease in protein and fat levels compared to the control. Moreover, the coating also suppresses therespiration rate, ethylene content, and MDA concentration during storage, especially for the Grobogan variety. The coated seed showeda higher vigor index and germination than the controls, particularly in the Dega variety. The CSNPs performed better than CS and controlfor all the experimental parameters. The germination percentage of the Dega variety soybean seeds after six months of storage at CS andCSNPs coating treatments were 93.33 and 92.67%, respectively, much higher than the Grobogan variety. The initial mositure contentmuch influenced the seed’s physical, chemical, and viability during storage.

Allelopathic effects of stems extracts of Dalbergia Paniculata Roxb.

Allelopathy activity of aqueous stems extracts of Dalbergia paniculata Roxb. using Vigna radiate L. and Zea mays L. seeds germination has been carried out using pot study. Dalbergia paniculata Roxb. showed positive allelopathy activity because of the large number of allelochemicals such as saponins present in aqueous stems extracts of Dalbergia paniculata Roxb. The investigated results suggested that aqueous stems extracts of Dalbergia paniculata Roxb. have showed positive allelopathic activity in seeds germination. The rate of seeds germination percentage and seedling vigor index increased by increasing the aqueous stems extracts concentration of Dalbergia paniculata Roxb. Therefore, stems extract of Dalbergia paniculata Roxb. can be used as natural fertilizer. By using natural fertilizer, crop production increases and shows better percentage of crop yields. The stems extract of Dalbergia paniculata Roxb. may act as herbicides, pesticides, fungicides because of large amounts of allelochemicals such as saponin present in plant products.