High relative humidity (RH) can cause lower concentrations of boron (B) accumulating in plants. The common greenhouse practice of controlling excess temperatures by applying mist irrigation to young plants (plugs) can result in elevated RH levels, especially with plugs grown in high heat and humidity conditions of summer. ‘Dynamite Yellow’ pansy (Viola ×wittrockiana Gams.), ‘White Storm’ petunia (Petunia ×hybrida Vilm.), and ‘Festival Apricot’ gerbera (Gerbera jamesonii Bolus) plugs were grown in high or ambient RH conditions to determine the effect RH had on B uptake. Results indicate that an increase in RH decreased the amount of water the plant lost as a result of transpiration resulting in lower concentrations of B in shoot tissue. Boron concentrations in leaf tissue were 9.43, 10.56, and 17.81 mg·L−1 in pansy, petunia, and gerbera plants, respectively, grown in high RH conditions. These values were significantly lower than pansy, petunia, and gerbera plants grown in ambient RH conditions (19.94, 25.49, and 42.71 mg·L−1, respectively). Leaf distortion, consistent with B deficiency symptoms, was present in petunia and gerbera plants. Similar trends were observed when the experiment was repeated and leaf distortion was present in all species. This provides convincing evidence that the distorted growth observed in pansy, petunia, and gerbera plug production is the result of limited B caused by excessive humidity.