Upland Rice Genotypes Research Articles

Clustering of upland rice genotypes by different biometrical methods

Clustering of upland rice genotypes by different biometrical methods

Limited effects of the soluble organic phosphorus fraction on the root phosphorus uptake efficiency of upland rice genotypes grown in acid soil

ABSTRACT The root phosphorus (P) uptake efficiency (RE), defined as plant P uptake per unit root mass or root area, may contribute to the P efficiency of upland rice grown in acid, P-deficient soils. The identification of root traits conferring RE of rice has been compromised by the lack of attention given to P speciation when evaluating P-mining mechanisms. Here we disentangled the effect of soluble organic P (PO) from that of total soluble P (PT) on the RE in rice seedlings in acid soil. Six rice genotypes were grown for 21 days in P-deficient substrates, i.e. a sample of an acid mineral soil, an acid peat and a mixture thereof. Each of the three substrates were amended with different P doses, partially limed and incubated, yielding substrates with significant differences in total soluble P (0.005–0.41 mg P/L) and in the percentage of organic P in that pool (PO/PT, 0–55%). Plants showed a large growth response to the P addition. There was a significant genotypic variation in P uptake (0.9–1.5mg P/plant) and RE (4.4–8.3 mg P/g root mass) under moderate P deficiency but these traits were unaffected by the PO/PT in the soluble fraction of the substrates. Along the same lines, phosphatase activity in the rhizosphere soil was unaffected by genotypes and did not explain the RE among all data (R2 = 0.17, ns). A multiple regression model showed that the RE of rice seedlings was mainly affected by the inherent genotypic effects, the PO/PT and the total soluble P concentration in soil (R2 = 0.73), while the genotype-PO/PT interaction only marginally improved the RE model (R2 = 0.78). This suggests limited genotypic effects due to the better use of organic P. A root elongation test in the acid mineral soil that was either or not limed suggests that the differences in acid soil tolerance may play a larger role in the genotypic performance of RE than organic P utilization potential.

New drought-tolerant rainfed upland rice (Oryza sp.) genotypes adapted to the west, centre-west, and centre regions of Côte d'Ivoire

To select new rainfed upland rice genotypes, adapted to the West, Centre-West, and Centre regions of Côte d'Ivoire, a study was conducted in research stations. Six genotypes (ART15-11-8-5-2-B-1, WAB891-SG12, WAB1092-B-40AB.1, ARCC3Fa3L10P1-1-B-1, and ART15-16-12 -3-1-B-1-B-3-1) including the control IDSA 10, widely cultivated across the country, were evaluated on three research stations of the National Center of Agricultural Research (CNRA), during the wet seasons of the year 2016 and 2017. These stations are located at the West, Center, and West-Center of Côte d'Ivoire. The trial was set up in a randomised complete block design with four replications. The agromorphological traits such as tillering ability, sowing-50% heading cycle, plant height, percentage of productive tillers, sowing-maturity cycle, and paddy yield were collected for each genotype. In all the environments evaluated, the genotypes ART15-11-8-5-2-B-1, WAB891-SG12, ARCC3Fa3L10P1-1-B-1, and ART15-16-12-3-1-B-1-B -3-1 were characterised by high percentages of productive tillers (87 to 91%), intermediate plant heights (114 to 121 cm), and high average paddy yields (2,601 to 2,810 kg/ha). Yield gains of these genotypes compared to the control ranged from 16 to 25%. The Genotype × Environment interaction (G × E) was highly significant for paddy yield (p < 0.001). The study of the interaction based on the first two principal components analysis of the GGE biplot, explained a 97% of the main effect of the Genotype and the G × E interaction. The polygon tool of the biplot suggested the existence of a single complex mega-environment. Visualizing the mean and stability of genotypes' paddy yield in the biplot indicated that genotypes ART15-11-8-5-2-B-1, WAB891-SG12, ARCC3Fa3L10P1-1-B-1, and ART15-16-12-3-1-B-1-B- 3-1 were more adapted to upland rice-growing regions of the West, Center-West, and Center of Côte d'Ivoire. These genotypes can be released for large scale rice production in these regions. Keywords: Rainfed upland rice, G × E interaction, GGE biplot analysis

Study on Genetic variability and character association for quality tratis in upland rice (Oryzasativa L.) genotypes

The experiment consisted of 34 high yielding rice genotype with one local check (NDR-359) collected from Department of Genetics & Plant breeding SHIATS, Allahabad grown at Field Experimental Centre of the Department of Genetic and Plant Breeding. Data were recorded on six quality traits viz., Hulling%, Kernal length before cooking, Kernel length after cooking, Kernel breadth before cooking, Kernel breadth after cooking and L/B Ratio.Analysis of variances indicated the existence of highly significant differences among the genotypes. On the basis of result characters which showed high genotypic value coupled with high heritability and genetic advance should be considered for direct selection. Here kernel length before cooking and kernel length after cooking showed high GCV, PCV, heritability and genetic advance. Thus one should select these characters for direct selection. Association of various quality traits like kernel length before cooking, kernel length after cooking and kernel breath after cooking would serve as a guideline for careful choice of parents for development of hybrids with better quality.

Characterization of indigenous upland rice varieties for high yield potential and grain quality characters under rainfed conditions in Thailand

Indigenous upland rice is a staple food for local people in the North and Northeast regions of Thailand. As a result, variations of grain yield and GxE interactions have been utilized for wider adaptability of upland rice varieties. A high yielding genotype that performs well under a good yielding environment as well as in poor environments is greatly needed. Our experiment, therefore, aimed to identify high potential indigenous upland rice varieties for grain yield and yield stability under rainfed conditions. Fifty upland rice genotypes were evaluated from 2013 to 2015, in which a randomized complete block design with three replications was laid out over the three years. Based on grain yield, eight indigenous upland rice varieties, including ULR026, ULR042, ULR075, ULR078, ULR080, ULR081, ULR089 and ULR105; demonstrated superior performance, high yield stability, and greater adaptability over the other varieties, including the Sew Mae Jan check variety. Additional qualities of the superior varieties included high amylose content (ULR081 and ULR075), high aroma (ULR078), and intermediate gelatinization temperature test (GT) (ULR078, ULR026, and ULR105). The participatory varietal selection (PVS) test for farmer(s) acceptance, and promotion of these varieties under rainfed conditions will be further studied.

Adaptabilidade e estabilidade fenotípica de linhagens de arroz em Minas Gerais

A alternativa adotada nos programas de melhoramento genético para diminuir o efeito da interação genótipo x ambiente (G x A), é através da avaliação das linhagens em uma rede de experimentos, sendo esses, conduzidos em vários anos e ambientes representativos das regiões edafoclimáticas de cultivo. Diante disso, o objetivo desse trabalho foi avaliar a adaptabilidade e estabilidade produtiva de genótipos de arroz de terras altas no estado de Minas Gerais, utilizando a ferramenta gráfica para análise de dados, o GGE biplot. Foram utilizados dados de produtividade de grãos, provenientes da avaliação de vinte linhagens de arroz do ensaio de Valor de Cultivo e Uso do programa de melhoramento de arroz de terras altas da Universidade Federal de Lavras em parceria com a Embrapa e Epamig. Os ensaios foram conduzidos em delineamento de blocos completos, com três repetições nas safras de 2013/2014, 2014/2015 e 2015/2016. Os experimentos foram conduzidos em um total de nove locais divididos dentro das três safras nas cidades de Lambari, Lavras e Patos de Minas, todas localizadas no estado de Minas Gerais. Concluiu-se com este trabalho que as linhagens foram em quase sua totalidade mais adaptadas e estáveis que as cultivares comerciais. Os genótipos 10 e 13 foram superiores à média geral para produtividade e apresentaram alta estabilidade, sendo candidatos futuros para lançamento como novas cultivares.

Farm yard manure application mitigates aluminium toxicity and phosphorus deficiency for different upland rice genotypes

AbstractUpland rice production on acid, weathered soils is often constrained by phosphorus (P) deficiency and aluminium toxicity. Farmyard manure application (FYM) can sharply enhance yields and agronomic P fertilizer (TSP) efficiency under such abiotic stresses. We tested the hypothesis that rice genotypes differ in aluminium tolerance and in the extent of using organic P, offering distinct benefits under TSP‐FYM combinations. Multiple field trials were conducted in the uplands of Madagascar, with factorial combinations of six genotypes, amendments of FYM (at a rate of 17–25 kg P ha−1 vs. a zero control) and TSP application (40 kg P ha−1 vs. a zero control), with blanket N&K additions. Rice grain yields reached a maximum of 6 t/ha after three years of TSP + FYM application, with an average of 3.2 t/ha over the years. Grain yields were about 1.2 t/ha for FYM only and about 1.5 t/ha for TSP only while crops failed under zero P input. Genotypic effects on rice yields were much smaller than the large effects of FYM, TSP or its combination. Application of FYM increased soil pH and CaCl2‐extractable P while decreasing CaCl2‐extractable aluminium. An additional liming trial indicated that the beneficial effects of FYM over TSP relate to liming effects. The FYM application lowers aluminium toxicity which overrules potential effects of organic P supply. Hence, genotypic ranking of yields and agronomic efficiency was inconsistent, without superior genotypes under FYM vs. TSP. However, Chomrong Dhan and FOFIFA 172 generally displayed superior yields. Chomrong Dhan is found to be more sensitive to aluminium toxicity whereas FOFIFA 172 is less performant under low P input. Aluminium tolerance should be considered when developing rice genotypes for high P efficiency in weathered soils.

Drought Tolerance and Nitrogen use Efficiency of Upland Rice (Oryza Sativa L.) Genotypes Grown under Varying Water and Nitrogen Regimes

Drought Tolerance and Nitrogen use Efficiency of Upland Rice (Oryza Sativa L.) Genotypes Grown under Varying Water and Nitrogen Regimes

Identification of quantitative trait loci underlying resistance and tolerance to the rice root-knot nematode, Meloidogyne graminicola, in Asian rice (Oryza sativa)

Meloidogyne graminicola is one of the most important plant-parasitic nematodes in rice. Breeding for natural resistance and tolerance is considered one of the most economical and sustainable approaches to control this damaging pathogen. Quantitative trait loci (QTL) mapping was carried out in a recombinant inbred line (RIL) population derived from a cross between the Asian rice genotypes IR78877-208-B-1-2 and Dinorado. IR78877-208-B-1-2 is an aerobic rice genotype that is resistant and tolerant to M. graminicola. Dinorado is a traditional upland rice genotype from the Philippines that is characterized by its aroma and prized for its cooking quality. The host response of 300 F4 lines derived from this cross was evaluated in both nematode infested and non-infested concrete beds in the dry seasons of 2013 and 2014. Five genotypes were found consistently resistant while 13 genotypes were consistently partially resistant to M. graminicola. QTL mapping for M. graminicola resistance/tolerance, yield-contributing traits, and yield revealed four main effect QTLs (qJ2RS2.1, qJ2GRT2.1, qJ2RS3.1, and qJ2GRT3.1) associated with reduced nematode reproduction on chromosomes 2 and 3 and two QTLs (qGR3.1 and qGR5.1) associated with reduced root galling on chromosomes 3 and 5. Twenty main effect QTLs associated with yield-contributing traits and yield were also found. Our study further identified co-locating QTLs associated with nematode resistance, yield-contributing traits, and yield on chromosomes 3 and 5. QTLs harboring candidate genes, such as OsBAK1, OsDST, OsIPT4, and DUF26, for higher grain yield and tolerance to abiotic and biotic stress, were identified. These QTLs and the RILs that are consistently resistant and tolerant to M. graminicola, and which are high-yielding in nematode-infested fields, can be utilized in breeding programs to improve the resistance and tolerance of Asian rice to this important pathogen.

Responses of Lowland, Upland and Aerobic Rice Genotypes to Water Limitation During Different Phases

Rice yield reduction due to water limitation depends on its severity and duration and on the phenological stage of its occurrence. We exposed three contrasting rice genotypes, IR64, UPLRi7 and Apo (adapted to lowland, upland and aerobic conditions, respectively), to three water regimes (puddle, 100% and 60% field capacity) in pots during the vegetative (GSI), flowering (GSII) and grain filling (GSIII) stages. Stress at all the three stages significantly reduced yield especially in lowland genotype IR64. Effect of water limitation was more severe at GSII than at the other two stages. Stress at GSI stage reduced both source activity (leaf area and photosynthetic rate) and sink capacity (tiller number or panicle number per pot). When stress was imposed at GSII, spikelet fertility was most affected in all the three genotypes. In both GSII and GSIII, although leaf area was constant in all the three water regimes, estimated relative whole-plant photosynthesis was strongly associated with yield reduction. Reduced photosynthesis due to stress at any given stage was found to have direct impact on yield. Compared to the other genotypes, Apo had deeper roots and maintained a better water relation, thus, higher carbon gain and spikelet viability, and ultimately, higher biomass and productivity under water-limited conditions. Therefore, screening for these stage-dependent adaptive mechanisms is crucial in breeding for sustained rice production under water limitation.

Relationship of root aquaporin genes, OsPIP1;3, OsPIP2;4, OsPIP2;5, OsTIP2;1 and OsNIP2;1 expression with drought tolerance in rice

Aquaporins are channel proteins that facilitate the passive uptake and transport of water, ions, molecules and regulate the water balance at the plant level. Genes for aquaporins are important since they play a major role in water uptake. The present study was therefore conducted to assess the relationship of aquaporins predominantly expressed in roots in response to drought tolerance. Transcript abundance of five rice aquaporin genes (PIP2;5, PIP1;3, PIP2;4, TIP2;1 and NIP2;1) in five rice genotypes (Banglami, ARC 10372, Inglongkiri, Ranjit and IR 64) under well-watered and drought stress conditions were measured using qRT-PCR. Significant variation in PIP relative transcript abundance in roots was observed between varieties under both well-watered and drought stress conditions. In roots, the expression levels of most OsPIPs were found to be increased in drought stress conditions. OsPIP1;3, OsPIP2;5, OsTIP2;1 and OsNIP2;1 were upregulated more in upland (drought tolerant) rice and Ranjit lowland (moderately drought tolerant) genotypes than drought susceptible lowland IR-64 rice in drought stress conditions and downregulated/unchanged in well-irrigated conditions. The OsPIP genes were identified as candidate genes based on their high expression under drought stress situations and played a significant role in water uptake and root development under water stress conditions. A positive correlation was observed between fold increase in aquaporin expression and degree of drought tolerance which suggest that these aquaporins genes might play an important role in root development under water stress situation to overcome stress.

Genetic Variability and Cluster Analysis for Phenological Traits of Thai Indigenous Upland Rice (Oryza sativa L.)

Estimating genetic variability and cluster analysis of grain yield and yield contributing traits need to require for rice breeders to choose the best breeding programs. Ten upland rice genotypes were conducted from farmers’ fields during the years of 2017 at three provinces of southern Thailand. Extreme broad sense heritability and genetic gain values for flag leaf length, leaf area index, harvest index, total dry weight and filled grains showed that assortment of these yield contributing traits would be effective. Cluster analysis categorized genotypes into three groups. In each group some genotypes such as Dawk Pa-yawm or Dawk Kha 50 (group I), Nahng Kian (group II) and Khao/ Trai (group III) showed that genotypes had different better traits. These studies revealed that high broad sense heritability traits and the best genotypes Nahng Kian and Khao/ Trai would be useful for improving new upland rice varieties in southern Thailand.

Brown spot disease severity, yield and yield loss relationships in pigmented upland rice cultivars from East Nusa Tenggara, Indonesia

Abstract. Mau YS, Ndiwa ASS, Oematan SS. 2020. Brown spot disease severity, yield and yield loss relationships in pigmented upland rice cultivars from East Nusa Tenggara, Indonesia. Biodiversitas 21: 1625-1634. Brown spot is one of the most devastating diseases of rice, which could lead to total yield loss. The disease has a worldwide distribution, more specifically in areas where water supply is scarce, most specifically in the dry upland areas. Almost all stages of rice are affected by the disease, where leaves and grains are mostly affected. Considerable differences exist in susceptibility to brown spot among rice varieties, which may cause a large variation in yield loss caused by the disease. Therefore, the resistance level of rice varieties and their yield reduction has to be regularly evaluated and updated. There are only a few reports on the relationship between brown spot severity with yield and yield loss of upland rice, and is even lacking in pigmented upland rice. The objectives of the present study were to assess the brown spot severity and resistance level in pigmented upland rice cultivars from East Nusa Tenggara Province, Indonesia, and to elucidate their relationships with yield and yield reduction. Twenty four pigmented upland rice genotypes were evaluated in the field during May to October 2019, and their disease responses and yields were recorded. Disease severity was observed weekly and used to calculate Area Under the Disease Progress Curve (AUDPC) for comparison among the genotypes. The relationships between disease severity and AUDPC with yield and yield loss were also examined. The results showed significant variation in brown spot severity and AUDPC, ranging from, respectively, 11.11% to 40.70% and 398.42%-days to 1081.30%-days. Yields and yield losses of test genotypes also varied substantially. Yields under diseased-free and diseased plots ranged from, respectively, 2.34 t ha-1 to 6.13 t ha-1 and 1.68 t ha-1 to 3.74 t ha-1 while yield loss was between 10.46% and 56.15%. Six genotypes were moderately resistant, four genotypes were moderately susceptible and 14 genotypes were susceptible to brown spot. Neither disease severity nor AUDPC had a linear relationship with yield but both exhibited positive and linear relationships with yield loss.

Effects of Nitrogen Rates on Morphological Development of Upland Rice Varieties in Mwea, Kirinyaga County, Kenya

Rice is the third most important cereal in consumption after maize and wheat in Kenya. It has been grown as a commercial food crop under irrigated and rain fed ecologies. Low soil Nitrogen (N) fertility is a major constraint to upland rice production in smallholder farmers mostly under rain fed agriculture. This study evaluated the morphological development of upland rice varieties as influenced by different N fertilizer treatments. A field study was carried out at the Kenya Agricultural and Livestock Research Organization experimental farm in Kirinyaga County for two seasons. The experiment was laid down as a randomized complete block design (RCBD) in split-plot arrangement in three replications. The main plot factors were the five upland rice genotypes, MWUR1, MWUR4, NERICA4, NERICA10 and IRAT109, while the subplots factors were the four N rates, 0, 26, 52 and 78 kg N ha-1. In addition calcium ammonium nitrate (26% N) was top-dressed in two equal splits at 21 and 45 days after sowing. Data on plant growth parameters was collected at fortnight interval. The analysis of variance revealed significant (p < 0.05) variation on plant height and tiller numbers due to N rates. However, there was no significant variation in plant height and tiller numbers due to variety. In addition, shoot and root dry weight and leaf area were significantly different due to variety and N rates in both seasons. Finally root dry weight was not significantly different due to variety and N-rates in the two seasons. The current study has shown that increase in nitrogen level lead to significant increase in plant height, tiller numbers, shoot dry weight and root dry weight. The study recommends the use of N fertilizers for rice cultivation to increase N supply in the soil.

Effect of iron deficiency on the growth, development and grain yield of some selected upland rice genotypes in the rainforest

Iron deficiency is a major production constraint of upland rice in the tropics despite is abundance in the soil. This investigation aimed to explicate the effect of iron deficiency on the growth, development, grain yield and its attributes of some selected upland rice in the rainforest. Field experiments were established at Africa Rice sub-Station, Ibadan, Nigeria. The treatments consisted of 35 upland rice genotypes and availability of iron in the soil (Fe-sufficient and Fe-deficient). The treatments were arranged in alpha lattice design with three replications. It was observed that upland rice sown in iron (Fe) deficient soils had significantly lower growth (plant height, number of tillers and seedling vigor), flowered later, with significantly lower yield attributes (1000 grain weight, filled grain) and grain yield than those sown in Fe-sufficient soils. Conversely, the number of unfilled grains were significantly higher in upland rice sown in Fe-deficient than those in sufficient soils. Percentage yield loss was in the range 98.00% to 22.95% for China best and Faro 65 respectively. Genotypes were identified to be tolerant (Faro 65, NERICA 3 and IRAT 109) and susceptible (Ofada 2, NERICA 5 and China Best) to Fe-deficiency based on their percentage grain yield loss. These evidences suggested that despite the increased phenology of upland rice sown in Fe-deficient soils their reproductive growth was suppressed through increased number of unfilled grains as witnessed in China Best and Faro 64.

Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa).

Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.

GGE Bi-Plot Analysis of Genotype by Environment Interaction in Medium Maturing Upland Rice Genotypes in Ethiopia

GGE Bi-Plot Analysis of Genotype by Environment Interaction in Medium Maturing Upland Rice Genotypes in Ethiopia

Performance evaluation and yield stability of upland rice (Oryza sativa L.) varieties in Ethiopia

Multi-environment evaluation of genotypes is important to identify the response of genotypes across environments and helps to know which genotypes would be appropriate in which production areas. Thirteen upland rice genotypes were evaluated in a randomized complete block design at Tepi, Assosa, Bako, and Bonga research centers in 2017, 2018, and 2019 cropping season with the objective of examining the agronomic performance and evaluating the yield stability of the varieties. The combined analysis of variance for yield and other agronomic characteristics revealed that main effects, genotypes, locations, years, and the three-way (G X L X Y) interaction effects revealed a significant difference for the measured traits. Moreover, AMMI analysis of variance also showed a highly significant difference for genotypes, environment, and genotype x environment (GE) interaction for grain yield. Environment effect (61.64%) was responsible for the greatest part of the variation, followed by genotype by environment interaction (20.86%) and genotype (6.97%) effects. The AMMI analysis explained 69.17% of the GxE variance, while GGE captured 72.76% of the GGE variance. Based on the GGE, AMMI analysis genotype variety Hidassie (G4), 4238.7 Kg ha−1 followed by NERICA-4 (G3), 4183.5 Kg ha−1 were the most stable and high yielding genotypes and recommended for production for the testing sites and similar upland rice-producing areas of Ethiopia.

English

Upland rice is an ecotype adapted to dry culture system. In this study, changes in germination characteristics and transcriptome of germinating seeds exposed to 15% polyethylene glycol (PEG) were investigated using upland rice genotype IRAT109. PEG reduced germination potential and inhibited growth of seed radicle and plumule. Under PEG stress, gibberellic acid (GA) content decreased and abscisic acid (ABA) content increased. Transcriptome analysis revealed that 1270 genes were differentially expressed between stressed and non-stressed seeds. Approximately, 56.46% of differentially expressed genes (DEGs) were up-regulated and 43.54% of DEGs were down-regulated under PEG stress. Gene Ontology (GO) analysis categorized 1149 DEGs into 52 functional groups and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis assigned 123 DEGs to 54 pathways. A large number of DEGs related to GA signal transduction, ABA biosynthesis and signal transduction, and defense response pathways were upregulated. Results indicated that genes related with GA signal transduction and ABA play important role in germination under drought stress. Findings of this study are highly useful to understand the molecular mechanism of germination and provide candidate genes for molecular breeding in dry direct-seeded rice. © 2019 Friends Science Publishers

Promising upland rice lines as new shading tolerant varieties with good grain quality

The utilization of coconut plantation land to increase rice planting area is a potential effort to maintain food sustainability in Indonesia. The development of shading tolerant rice varieties has been undertaken through plant breeding programs. Preliminary yield trial of upland rice genotypes under coconut plantation with 50% light intensity have been carried out in the village of Cilincing, Tegalbulued District, Sukabumi in the WS 2017. The genotype consists of 36 rice lines 4 check varieties, i.e Inpago 8, Limboto, Situpatenggang, and Jatiluhur. The result showed that the height of the lines ranged from 70.8 cm - 111.1 cm and the number of productive tillers were between 3 - 11.9 tillers. The line B15341-1B-TB-29 showed the earliest flowering time of 83 days after sowing (DAS). The panicle length of rice genotypes ranged from 29.4 cm - 21.8 cm and the average weight of 1000 grains of the line was 25.7 g. The ranges of filled grain per panicle of the lines were 47-157 grains. B14908C-MR-1-25-1-3 had the highest amount of the number of filled grain per panicle. Amylose levels of those genotypes were in the range of 17-22% and the rice texture was sticky to medium. The shape and size of the rice were long to medium. There were six promising rice, namely B14981B-TGB-4, B15344B-TB-30, B15175C-TGB-39, B15209B-MR-17-1, B14908C-MR-1-25-1-3, and B14192E-MR-33 that have the potential to be released as new shade-tolerant varieties.