VPM 1, a bread wheat with eyespot resistance derived from Aegilops ventricosa was shown to have a characteristic α-amylase allele on chromosome 7D, α-Amy-D2b also derived from Ae. ventricosa. Chromosome substitution lines confirmed that the eyespot resistance of VPM 1 was also determined by chromosome 7D. Among F3 families from a cross between VPM 1 and a line lacking this eyespot resistance and the α-amylase marker allele, the two characters segregated independently. This finding reduces the value of the allele as a marker for eyespot resistance, but suggests that VPM 1 possesses a large segment of chromosome from the D genome of Ae. ventricosa.