Human papillomavirus (HPV) is a DNA tumor virus that is the primary cause of sexually transmittedinfections (STIs). The present study aimed to to evaluate the Effect of nursing guideline about genital Humanpapilloma virus infection on Knowledge and attitudeof female university students’.Sample Type: A convenience sampling technique. Research design: Quasi-experimental design was used inthis study. Setting: at Faculty of nursing in Alexandria University.Sample Size: Total sample size was two hundred seventy five (275) female students. Data was collectedby, 1): An Arabic structured Interviewing Questionnaire. 2) Follow up sheet and 3) likert Scale.Results: theresult of the present study findings had revealed that a highly significant improvement in total knowledge andattitude among the studied sample pre–nursing guideline compared to immediate and 6 months post- nursingguideline P = < 0.01. The majority of studied sample satisfied with the advanced knowledge included in thenursing guideline.Conclusion: The present study findings concluded that a significant improvement among studied sample’knowledge andattitude after implementation of guideline.Recommendations: There is a need for awareness campaigns to improve the poor knowledge of femaleuniversity students in order to change behavior and reduce risk of HPV infection so that they will take theHPV vaccine. As a step towards prevention of cervical cancer.