You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2015MP52-02 REGENERATION OF RAT CORPUS CAVERNOSA TISSUE AFTER TRANSPLANTATION OF CD 133+ CELLS DERIVED FROM HUMAN BONE MARROW AND PLACEMENT OF BIODEGRADABLE GEL SPONGE SHEET. Shogo Inoue, Shunsuke Shinmei, Koichi Shoji, Mitsuru Kajiwara, Jun Teishima, and Akio Matsubara Shogo InoueShogo Inoue More articles by this author , Shunsuke ShinmeiShunsuke Shinmei More articles by this author , Koichi ShojiKoichi Shoji More articles by this author , Mitsuru KajiwaraMitsuru Kajiwara More articles by this author , Jun TeishimaJun Teishima More articles by this author , and Akio MatsubaraAkio Matsubara More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1719AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Conditions, such as congenital anomalies, penile cancer, traumatic penile injury and some types of vasculogenic erectile dysfunction (ED), often require penile reconstruction. The objective of this study was to develop an easier regenerating technique of corpus cavernosa through transplanting human bone-marrow-derived CD133+ cells into a rat corpus cavernosa defect model. METHODS 2x2 mm squares of the right corpus cavernosa of 8-week-old male nude rats were excised. Alginate gel sponge sheets supplemented with 1x104 CD133+ cells derived from human bone marrow were then placed over the gaps on both sides (CD group). The same experiments were conducted on sham-operated rats (SH group), rats with only corpus cavernosa excision (EX group), and rats with alginate gel sheets placed on the injured corpus cavernosa (AL group). Functional and histological evaluations were carried out eight weeks later. We used anti α-smooth muscle actin as a primary antibody to detect vascular smooth muscle. We also used anti-S-100 protein as a primary antibody to detect neuron tissue, especially Schwann cells. In the AL and CD groups, four days after the corpus cavernosa were excised and an alginate gel sheet was placed over the corpus cavernosa stumps, the sheets on the only the right side of the corpus cavernosa were retrieved from three rats in each group. We carried out quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of the rat nerve growth factor (NGF) and rat vascular endothelial growth factor (VEGF) in the alginate gel sheets. RESULTS The mean intracavernous pressure/mean arterial pressure of the CD group (0.34258 ± 0.0831) was significantly higher than that of the EX group (0.0580 ± 0.0831; P = 0.0238) and similar to that of the SH group (0.37228 ± 0.1051; P = 0.8266). Immunohistochemical analysis revealed some venous-sinus-like structures without vascular smooth muscle and neuron tissues were regenerated in the CD group. The RT-PCR for the extracts from the alginate gel sponge sheets retrieved in the CD group revealed that qRT-PCR helped in determining the amounts of mRNA for rat NGF and rat VEGF to be significantly higher than those in the AL group (NGF: P = 0.0309, VEGF: P < 0.0001). CONCLUSIONS Transplantation of CD133+ cells accelerated the functional and histological recovery in this corpus cavernosa defect model. We believe CD133+ cell transplantation will be able to be applied to the treatment of penile regeneration or severe ED that is not responsive to PDE5 inhibitors. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e628 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Shogo Inoue More articles by this author Shunsuke Shinmei More articles by this author Koichi Shoji More articles by this author Mitsuru Kajiwara More articles by this author Jun Teishima More articles by this author Akio Matsubara More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...