Gel Phantom Research Articles

Noninvasive Ablation of Prostate Cancer Spheroids Using Acoustically-Activated Nanodroplets.

We have developed acoustically activated nanodroplets (NDs) using an amphiphilic triblock copolymer, which self-assembles and encapsulates different perfluorocarbons including perfluoropentane (PFP) and perfluorohexane (PFH). Applying histotripsy pulses (i.e., short, high pressure, ultrasound pulses) to solutions of PFP- and PFH-NDs generated bubble clouds at a significantly reduced acoustic pressure compared to the cavitation pressure observed for histotripsy treatment alone. In this report, we summarize the results of combining histotripsy at low frequency (345 and 500 kHz) with PFP-NDs and PFH-NDs on the ablation of PC-3 and C4-2B prostate cancer cells. Using custom built histotripsy transducers coupled to a microscope and a high speed recording camera, we imaged the generation of a cavitation bubble cloud in response to different ultrasound regimes in solution and in tissue-mimicking gel phantoms. We quantified the associated ablation of individual cancer cells and 3D spheroids suspended in solution and embedded in tissue phantoms to compare the ablative capacity of PFP-NDs and PFH-NDs. Results show that histotripsy pulses at high acoustic pressure (26.2 MPa) ablated 80% of prostate cancer spheroids embedded in tissue-mimicking gel phantoms. In comparison, combining histotripsy pulses at a dramatically lower acoustic pressure (12.8 MPa) with PFP-NDs and PFH-NDs caused an ablation of 40% and 80% of the tumor spheroid volumes, respectively. These results show the potential of acoustically activated NDs as an image-guided ablative therapy for solid tumors and highlight the higher ablative capacity of PFH-NDs, which correlates with the boiling point of the encapsulated PFH and the stability of the formed bubble cloud.

Shear Wave Imaging of Breast Tissue by Color Doppler Shear Wave Elastography.

Shear wave elastography is a distinctive method to access the viscoelastic characteristic of the soft tissue that is difficult to obtain by other imaging modalities. This paper proposes a novel shear wave elastography [color Doppler shear wave imaging (CD SWI)] for breast tissue. Continuous shear wave is produced by a small lightweight actuator, which is attached to the tissue surface. Shear wave wavefront that propagates in tissue is reconstructed as a binary pattern that consists of zero and the maximum flow velocities on color flow image (CFI). Neither any modifications of the ultrasound color flow imaging instrument nor a high frame rate ultrasound imaging instrument is required to obtain the shear wave wavefront map. However, two conditions of shear wave displacement amplitude and shear wave frequency are needed to obtain the map. However, these conditions are not severe restrictions in breast imaging. This is because the minimum displacement amplitude is [Formula: see text] for an ultrasonic wave frequency of 12 MHz and the shear wave frequency is available from several frequencies suited for breast imaging. Fourier analysis along time axis suppresses clutter noise in CFI. A directional filter extracts shear wave, which propagates in the forward direction. Several maps, such as shear wave phase, velocity, and propagation maps, are reconstructed by CD SWI. The accuracy of shear wave velocity measurement is evaluated for homogeneous agar gel phantom by comparing with the acoustic radiation force impulse method. The experimental results for breast tissue are shown for a shear wave frequency of 296.6 Hz.

Evaluation of a small flat rectangular therapeutic ultrasonic transducer intended for intravascular use

BackgroundThe aim of the proposed study was to evaluate the performance of a flat rectangular (2×10mm2) transducer operating at 4MHz. The intended application of this transducer is intravascular treatment of thrombosis and atherosclerosis. MethodsThe transducer’s thermal capabilities were tested in two different gel phantoms. MR thermometry was used to demonstrate the thermal capabilities of this type of transducer. ResultsTemperature measurements demonstrated that this simple and small transducer adequately produced high temperatures, which can be utilized for therapeutic purposes. These high temperatures were confirmed using thermocouple and MR measurements. Pulsed ultrasound in combination with thrombolytic drugs and microbubbles was utilized to eliminate porcine thrombi. ConclusionsThe proposed transducer has the potentials to treat atherosclerotic lesions using the thermal properties of ultrasound, since high temperatures can be achieved in less than 5s. The results revealed that the destruction of thrombi using pulsed ultrasound requires long exposure time and high microbubble dosage.

A Fiber Optic Probe for Tumor Laser Ablation With Integrated Temperature Measurement Capability

This paper presents the characterization results of a new all-optical applicator for improved tumor laser ablation treatments that features customized irradiation pattern and built-in temperature sensors. The probe exploits a double cladding optical fiber to integrate some Bragg gratings acting as temperature sensing elements in the core while guiding the high power beam used for the ablation in the inner cladding. The assessment of the probe behavior has been conducted in two steps: First, with an agar gel phantom to characterize the irradiation pattern and to validate the Bragg grating based measurement setup in comparison with a thermographic camera; then, simulating actual treatments using an ex vivo animal liver.

Modeling the cutting edge geometry of scalpel blades

Scalpel blades are commonly used in surgery to perform invasive medical procedures, yet there has been limited research on the geometry that makes up these cutting instruments. The goal of this article is to define scalpel blade geometry and examine the cutting forces and deflection between commonly used scalpel blades and phantom gel. The following study develops a generalized geometric model that describes the cutting edge geometry in terms of normal rake and inclination angle of any continuously differentiable scalpel cutting edge surface. The parameter of scalpel-tissue contact area is also examined. The geometry of commonly used scalpel blades (10, 11, 12, and 15) is compared to each other and their cutting force through phantom gel measured. It was found that blade 10 displayed the lowest average total steady-state cutting force of 0.52 N followed by blade 15, 11, and 12 with a cutting force of 1.17 N (125% higher than blade 10). Blade 10 also displayed the lowest normalized cutting force of 0.16 N/mm followed by blades 15, 12, and 11 with a force of 0.19 N/mm (17% higher than blade 10).

Tissue lesion formation induced by high intensity focused ultrasound along a spiral pathway

Both theoretical and experimental studies were performed here to investigate the lesion formation induced by high-intensity focused ultrasound (HIFU) operating in continuous scanning mode along a spiral pathway. The Khokhlov-Zabolotskaya-Kuznetsov equation and bio-heat equation were combined in the current model to predict HIFU-induced temperature distribution and lesion formation. The shape of lesion and treatment efficiency were assessed for a given scanning speed at two different grid spacing (3 mm and 4 mm) in both the gel phantom and ex vivo studies. The results show that uniform lesions can be generated by the homogenization of thermal diffusion along the spiral scanning pathway. The complete coverage of the entire treated volume can be achieved as long as the spacing grid of the spiral pathway is smaller than a critical value that right matches the maximum thermal diffusion dimension, and the treatment efficiency can be optimized by selecting an appropriate scanning speed. This study can provide guidance for further optimization of the treatment efficiency of HIFU therapy.

Dependence of inertial cavitation activity on the high intensity focused ultrasound transducer F-number

Cavitation induced in tumors by pulsed high intensity focused ultrasound (pHIFU) without ultrasound contrast agents was shown to significantly enhance chemotherapeutic drug uptake. Such de novo cavitation is commonly assumed to require very high rarefactional pressures. However, recent studies have shown that inertial cavitation threshold also correlates with formation of shocks at the focus. The shock amplitude and corresponding peak negative pressure (p-) are primarily determined by the transducer F-number with less focused transducers producing shocks at lower p-. Here, the dependence of inertial cavitation activity on F-number was investigated in gel phantoms and ex vivo tissue samples using passive cavitation detection (PCD). Exposures at 1.5 MHz consisted of 60 pulses delivered at 1 Hz PRF, with each pulse lasting 1 ms and p- from 1 to 15 MPa. Broadband noise emissions recorded by PCD were batch-processed to extract cavitation probability and persistence. At the same p-, both metrics indicate enhanced cavitation activity at higher F-numbers; in agarose phantoms, cavitation probability reached 100% when shocks formed at the focus, with p- values 5, 9, and 14.5 MPa for respective F-numbers 1.5, 1, and 0.75. These results confirm the impact of nonlinear waveform distortion on inertial cavitation. [Work supported by NIH K01EB015745, K01DK104854, and R01EB7643.]

98 Ultrasound-Guided Nerve Blocks: A Comparison of Two Teaching Methods: Traditional Identification of Nerves Versus Hands-on Practice With a Gel Phantom Model

98 Ultrasound-Guided Nerve Blocks: A Comparison of Two Teaching Methods: Traditional Identification of Nerves Versus Hands-on Practice With a Gel Phantom Model

Tissue boundary roughness index for evaluating malignancy of cancer in ultrasound-computed tomography system

Ultrasound-computed tomography (USCT) is a promising candidate for a radiation free, painless, and quantitative modality for breast cancer examination. We developed a USCT prototype with a ring-shaped transducer array (frequency: 1.5 MHz) that moves along the ring axis for scanning the entire breast. Distributions of speed and attenuation of ultrasound are computed from ultrasound transmitted through tissue. Although prior research shows that there is a correlation between malignancy and the speed of sound and attenuation values, in some cases both benign and malignant tumors have similar values. In this study, we propose to quantify the boundary roughness of a tumor from the spatial power distributions of reflected ultrasounds that are transmitted from different apertures of the ring transducer. In conventional ultrasound echography, the boundary roughness of a tumor is an important indicator of malignancy. However, roughness is judged by radiologists based on B-mode images and is a subjective index. We simulated the ultrasound reflection signal from boundaries of different roughness. The simulation result indicates that a spatial power concentration has a significant correlation with boundary roughness. We confirmed through gel phantom experiments that the index distinguishes rough and smooth boundaries, which is not evident in B-mode images.

Dosimetric validation for an automatic brain metastases planning software using single-isocenter dynamic conformal arcsDosimetric validation for an automatic brain metastases planning software using single-isocenter dynamic conformal arcs.

An automatic brain‐metastases planning (ABMP) software has been installed in our institution. It is dedicated for treating multiple brain metastases with radiosurgery on linear accelerators (linacs) using a single‐setup isocenter with noncoplanar dynamic conformal arcs. This study is to validate the calculated absolute dose and dose distribution of ABMP. Three types of measurements were performed to validate the planning software: 1, dual micro ion chambers were used with an acrylic phantom to measure the absolute dose; 2, a 3D cylindrical phantom with dual diode array was used to evaluate 2D dose distribution and point dose for smaller targets; and 3, a 3D pseudo‐in vivo patient‐specific phantom filled with polymer gels was used to evaluate the accuracy of 3D dose distribution and radiation delivery. Micro chamber measurement of two targets (volumes of 1.2 cc and 0.9 cc, respectively) showed that the percentage differences of the absolute dose at both targets were less than 1%. Averaged GI passing rate of five different plans measured with the diode array phantom was above 98%, using criteria of 3% dose difference, 1 mm distance to agreement (DTA), and 10% low‐dose threshold. 3D gel phantom measurement results demonstrated a 3D displacement of nine targets of 0.7±0.4 mm (range 0.2 ~ 1.1 mm). The averaged two‐dimensional (2D) GI passing rate for several region of interests (ROI) on axial slices that encompass each one of the nine targets was above 98% (5% dose difference, 2 mm DTA, and 10% low‐dose threshold). Measured D95, the minimum dose that covers 95% of the target volume, of the nine targets was 0.7% less than the calculated D95. Three different types of dosimetric verification methods were used and proved the dose calculation of the new automatic brain metastases planning (ABMP) software was clinical acceptable. The 3D pseudo‐in vivo patient‐specific gel phantom test also served as an end‐to‐end test for validating not only the dose calculation, but the treatment delivery accuracy as well.PACS number(s): 87.53.Lv, 87.55.km, 87.55.Qr

Mechanical analysis of an axially symmetric cylindrical phantom with a spherical heterogeneity for MR elastography

Cylindrical homogenous phantoms for magnetic resonance (MR) elastography in biomedical research provide one way to validate an imaging systems performance, but the simplified geometry and boundary conditions can cloak complexity arising at tissue interfaces. In an effort to develop a more realistic gel tissue phantom for MRE, we have constructed a heterogenous gel phantom (a sphere centrally embedded in a cylinder). The actuation comes from the phantom container, with the mechanical waves propagating toward the center, focusing the energy and thus allowing for the visualization of high-frequency waves that would otherwise be damped. The phantom was imaged and its stiffness determined using a 9.4 T horizontal MRI with a custom build piezo-elastic MRE actuator. The phantom was vibrated at three frequencies, 250, 500, and 750 Hz. The resulting shear wave images were first used to reconstruct material stiffness maps for thin (1 mm) axial slices at each frequency, from which the complex shear moduli μ were estimated, and then compared with forward modeling using a recently developed theoretical model which took μ as inputs. The overall accuracy of the measurement process was assessed by comparing theory with experiment for selected values of the shear modulus (real and imaginary parts). Close agreement is shown between the experimentally obtained and theoretically predicted wave fields.

Creating a tissue mimicking phantom appropriate for relaxometry, diffusion imaging and ultrasound elastography

Introduction To optimize elastography and diffusion weighted imaging (DWI) modalities, it is important to create phantoms that can reliably mimic the properties of human tissue and be used in reference imaging experiments. In this study, we used polyvinyl alcohol cryogel (PVA-C), agar, and agarose gels. Purpose To create a tissue mimicking phantom appropriate for relaxometry, non-Gaussian diffusion imaging and ultrasound elastography and to investigate potential relationships between quantities accessible from different imaging modalities for cancer patients such as prostate cases. Materials and methods Homogenous gel phantoms were created in different concentrations to set MRI and elastography parameters to be consistent with those found in healthy and diseased prostate tissue. Images were acquired on a 3T scanner for relaxometry and DWI studies. In-house software (MATLAB) was used to analyse the images on a pixel-by-pixel basis. Shear Wave Elastography was used to obtain speed and Young’s Modulus of the phantoms. Results As qualitative and quantitative assessments, ultrasound and CT were used and the results were identical for each gel phantoms, confirming homogeneity in addition to slice profiles and field maps obtained from MRI. Relaxation times and diffusion coefficients of the phantoms were found as compatible with the previous studies in the literature. A good correlation was observed in agar gels on their elasticity than other phantoms. Conclusion Our gel phantoms were developed and evaluated for MRI diffusion and elastography imaging modalities and design to mimic tissue parameters for prostate cancer. The phantoms may also model other tissues to various degrees. Disclosure ZGP would like to acknowledge TUBITAK for her financial support with a Project No. 1059B141400677 and Research Fund of Cukurova University , Project No. FDK-2014-2709 during this work. SS acknowledges funding from a Royal Society Leverhulme Trust Senior Fellowship.

Effect of hydrodynamic cavitation in the tissue erosion by pulsed high-intensity focused ultrasound (pHIFU)

High-intensity focused ultrasound (HIFU) is emerging as an effective therapeutic modality in clinics. Besides the thermal ablation, tissue disintegration is also possible because of the interaction between the distorted HIFU bursts and either bubble cloud or boiling bubble. Hydrodynamic cavitation is another type of cavitation and has been employed widely in industry, but its role in mechanical erosion to tissue is not clearly known. In this study, the bubble dynamics immediately after the termination of HIFU exposure in the transparent gel phantom was captured by high-speed photography, from which the bubble displacement towards the transducer and the changes of bubble size was quantitatively determined. The characteristics of hydrodynamic cavitation due to the release of the acoustic radiation force and relaxation of compressed surrounding medium were found to associate with the number of pulses delivered and HIFU parameters (i.e. pulse duration and pulse repetition frequency). Because of the initial big bubble (~1 mm), large bubble expansion (up to 1.76 folds), and quick bubble motion (up to ~1 m s−1) hydrodynamic cavitation is significant after HIFU exposure and may lead to mechanical erosion. The shielding effect of residual tiny bubbles would reduce the acoustic energy delivered to the pre-existing bubble at the focus and, subsequently, the hydrodynamic cavitation effect. Tadpole shape of mechanical erosion in ex vivo porcine kidney samples was similar to the contour of bubble dynamics in the gel. Liquefied tissue was observed to emit towards the transducer through the punctured tissue after HIFU exposure in the sonography. In summary, the release of HIFU exposure-induced hydrodynamic cavitation produces significant bubble expansion and motion, which may be another important mechanism of tissue erosion. Understanding its mechanism and optimizing the outcome would broaden and enhance HIFU applications.

Imaging and quantification of iron‐oxide nanoparticles (IONP) using MP‐RAGE and UTE based sequences

To investigate two-dimensional (2D) and three-dimensional (3D) ultrashort echo time (UTE) and 3D magnetization-prepared rapid gradient-echo (MP-RAGE) sequences for the imaging of iron-oxide nanoparticles (IONP). The phantoms were composed of tubes filled with different IONP concentrations ranging from 2 to 45 mM. The tubes were fixed in an agarose gel phantom (0.9% by weight). Morphological imaging was performed with 3D MP-RAGE, 2D UTE, 2D adiabatic inversion recovery-prepared UTE (2D IR-UTE), 3D UTE with Cones trajectory (3D Cones), and 3D IR-Cones sequences. Quantitative assessment of IONP concentration was performed using R2*(1/T2*) and R1 (1/T1 ) measurements using a 3 Tesla (T) scanner. The 3D MP-RAGE sequence provides high-contrast images of IONP with concentration up to 7.5 mM. Higher IONP concentration up to 37.5 mM can be detected with the UTE sequences, with the highest IONP contrast provided by the 3D IR-Cones sequence. A linear relationship was observed between R2* and IONP concentration up to ∼45 mM, and between R1 and IONP concentration up to ∼30 mM. The clinical 3D MP-RAGE sequence can be used to assess lower IONP concentration up to 7.5 mM. The UTE sequences can be used to assess higher IONP concentration up to 45 mM. Magn Reson Med 78:226-232, 2017. © 2016 International Society for Magnetic Resonance in Medicine.

Cavitation-induced damage of soft materials by focused ultrasound bursts: A fracture-based bubble dynamics model.

A generalized Rayleigh-Plesset-type bubble dynamics model with a damage mechanism is developed for cavitation and damage of soft materials by focused ultrasound bursts. This study is linked to recent experimental observations in tissue-mimicking polyacrylamide and agar gel phantoms subjected to bursts of a kind being considered specifically for lithotripsy. These show bubble activation at multiple sites during the initial pulses. More cavities appear continuously through the course of the observations, similar to what is deduced in pig kidney tissues in shock-wave lithotripsy. Two different material models are used to represent the distinct properties of the two gel materials. The polyacrylamide gel is represented with a neo-Hookean elastic model and damaged based upon a maximum-strain criterion; the agar gel is represented with a strain-hardening Fung model and damaged according to the strain-energy-based Griffith's fracture criterion. Estimates based upon independently determined elasticity and viscosity of the two gel materials suggest that bubble confinement should be sufficient to prevent damage in the gels, and presumably injury in some tissues. Damage accumulation is therefore proposed to occur via a material fatigue, which is shown to be consistent with observed delays in widespread cavitation activity.

Design and Verification of Novel Low-Cost MR-Guided Small-Animal Stereotactic System

Stereotactic systems have been used for decades to position surgical instruments in important preclinical and clinical studies. We developed and verified a low-cost magnetic-resonance-guided system for accurate preclinical stereotactic operations on small animals. The system consists of both commercially available and custom components, including in-house software written in MATLAB to register coordinate spaces. The software registers target the image-space coordinates from magnetic resonance imaging to the physical space through a rigid-body transform. The transform uses singular value decomposition to determine rotation and translation matrices that are optimal in a least squares sense. Needle positioning and core biopsy studies were performed to verify the accuracy of the system. The needle positioning study demonstrated a sub-millimeter positioning error of 0.294 ± 0.156 mm while using a 26 s gauge Hamilton needle. Biopsy studies demonstrated >94 % success in accessing sub-millimeter stereotactic targets embedded in agarose gel phantoms. These favorable results confirm that despite its relatively low hardware cost (US$18,730) and easy assembly, the system is able to consistently access stereotactic targets on the spatial scale of a small animal.

In vivo setup characterization for pulsed electromagnetic field exposure at 3 GHz

An in vivo setup for pulsed electric field exposure at 3 GHz is proposed and characterized in this work. The exposure system allows far field, whole-body exposure of six animals placed in Plexiglas cages with a circular antenna. Chronic exposures under 18 W incident average power (1–4 kW peak power) and acute exposures under 56 W incident average power (4.7 kW peak power) were considered. Numerical and experimental dosimetry of the setup allowed the accurate calculation of specific absorption rate (SAR) distributions under various exposure conditions. From rat model numerical simulations, the whole-body mean SAR values were 1.3 W kg−1 under chronic exposures and 4.1 W kg−1 under acute exposure. The brain-averaged SAR value was 1.8 W kg−1 and 5.7 W kg−1 under chronic and acute exposure, respectively. Under acute exposure conditions, a 10 g specific absorption of 1.8 ± 1.1 mJ · kg−1 value was obtained. With temperature rises below 0.8 °C, as measured or simulated on a gel phantom under typical in vivo exposures, this exposure system provides adequate conditions for in vivo experimental investigations under non-thermal conditions.

A USPIO doped gel phantom for R2* relaxometry.

This work describes a phantom containing regions of controlled R2* (1/T2*) values to provide a stable reference object for testing implementations of R2* relaxometry commonly used for liver and heart iron assessments. A carrageenan-strengthened gadolinium DTPA doped agarose gel was used to enclose nine gels additionally doped with ultra-small superparamagnetic iron oxide. R2* values were determined at 1.5 T using multi-echo GRE sequences and exponential regression of pixel values from a region of interest against echo time using non-linear regression algorithms. We measured R2*, R2 and R1 values and the inter-scan and inter-operator reproducibility. The phantom reliably demonstrated R2* values in seven steps between 22.4s-1 (SE 1.98) and 441.9s-1 (SE 6.76), with an R2* relaxivity (r2*) of 792 (SE 5.6) mM-1s-1. The doped gels displayed a concentration-dependent R2' component of R2* phantom, indicating superparamagnetic enhancement effects. We observed no significant change in relaxivity (r2*) over 12months, and estimate a useful life of 3years. Detailed descriptions of the production process and calculators are been provided as Online Resources. The phantom provides a durable test object with controlled R2* relaxation behaviour, useful for a range of R2* relaxometry reference work.

Preparation and evaluation of MRI detectable poly (acrylic acid) microspheres loaded with superparamagnetic iron oxide nanoparticles for transcatheter arterial embolization

To monitor the spatial distribution of embolic particles inside the target tissues during and after embolization, blank poly (acrylic acid) microspheres (PMs) were initially prepared by inverse suspension polymerization method and then loaded with superparamagnetic iron oxide (SPIO) nanoparticles by in situ precipitation method to obtain magnetic resonance imaging (MRI) detectable SPIO-loaded poly (acrylic acid) microspheres (SPMs). The loading of SPIO nanoparticles in SPMs was confirmed by vibrating sample magnetometer, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and infrared spectrum, respectively. The results showed that SPMs exhibited excellent superparamagnetism and the SPIO embedded in SPMs were proved to be inverse spinel magnetite. The content of SPIO loaded in wet SPMs of subgroups of 100–300, 300–500, 500–700 and 700–900μm was measured to be 11.84±0.07, 10.20±0.05, 9.98±0.00 and 8.79±0.01mg/ml, corresponding to the weight percentage in freeze-dried SPMs to be 18.07±0.28%, 18.54±0.13%, 18.66±0.01% and 18.50±0.07%, respectively. The SPMs were spherical in shape, had smooth surface, and were within the size range of clinical demands for embolization. The compression tests indicated that SPMs were more rigid than PMs and commercially used Embospheres (P<0.01). The MRI detectability of SPMs was evaluated with the SPMs embedded in gel phantom in vitro and injected subcutaneously into the back of mice in vivo. Both the results demonstrated that the SPMs could provide distinct negative contrast enhancement and be sensitively detected by T2-weighted MR imaging. All the results show that SPMs are potential MRI detectable embolic microspheres for the future embolotherapy.

Abstract 1312: PSMA antibody functionalized docetaxel-loaded magnetic nanoparticles for prostate cancer therapy

Abstract Objectives: Prostate cancer (PrCa) is the second most leading cause of cancer-related death in men in the United States. Chemotherapy (Docetaxel, Dox) is currently the most common first-line therapeutic option. However, adverse side effects and chemo-resistance of docetaxel limit its clinical use. Improving docetaxel targeted delivery and its activity at the tumor site using a targeted nanoparticle system could be an attractive strategy for PrCa therapy. Prostate Specific Membrane Antigen (PSMA) is highly overexpressed in PrCa cells, thus is a highly attractive molecular target for PrCa therapy. In this study, we developed and determined anti-cancer efficacy of a novel docetaxel loaded, PSMA targeted magnetic nanoparticle (PSMA-MNP-Dox) formulation for PrCa therapy. Methods: Docetaxel loaded magnetic nanoparticle (MNP-Dox) formulation is composed of an iron oxide core coated with cyclodextrin (for drug loading) and F127 polymer (for particle stability and chemosensitization). Therapeutic efficacy of this unique nanoparticle formulation was evaluated using clinically relevant cell line models (C4-2, PC-3, and DU-145) through cell proliferation and colony formation assays. Molecular effects of this formulation on apoptosis, anti-apotosis, and drug resistance associated proteins were evaluated using immunoblotting assays. Contrast imaging property of MNP-Dox formulation was examined using Phantom Gel MR imaging model. For active targeting, PSMA antibody conjugation to this formulation was achieved through N-hydroxysuccinimide group containing PEG polymer. Active targeting potential of this formulation was evaluated in PSMA+ (C4-2) and PSMA- (PC-3) cell lines, C4-2 generated tumor xenografts. Results: MNP-Dox formulation showed optimal particle size and zeta potential which can efficiently internalized in PrCa cells. Our formulation showed anti-cancer efficacy in prostate cancer cell lines. Additionally, it induces the expression of apoptosis associated proteins, Bax and Bad, cleaved PARP, and caspase 3, and downregulated the expression of anti-apoptotic proteins, Bcl-2 and Bcl-xL. Moreover, it also inhibited the expression of chemoresistance associated proteins (PSMA and MDR1). Our PSMA antibody targeted MNPs-Dox formulation exhibited a profound uptake pattern in PSMA+ cells (C4-2) compared to PSMA null (PC-3)- cells, suggesting its targeting potential. A similar targeting potential was also observed in ex-vivo studies while using C4-2 tumor xenografts, however, no intense targeting was observed in normal tissues due to lack of PSMA expression. Conclusion: PSMA antibody functionalized MNP-Dox formulation can efficiently target PSMA + PrCa cells and deliver docetaxel into prostate tumors. This targeted drug delivery system could reduce the dose of docetaxel required to kill cancer cells, thus minimizing long-term docetaxel associated systemic toxicity and drug-resistance. Citation Format: Prashanth Kumar Bhusetty Nagesh, Nia Johnson, Vijaya K.N. Boya, Pallabita Chowdhury, Aditya Ganju, Bilal Hafeez, Sheema Khan, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu. PSMA antibody functionalized docetaxel-loaded magnetic nanoparticles for prostate cancer therapy. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1312.