Cold Seeps Research Articles

Thaumarchaeota from deep-sea methane seeps provide novel insights into their evolutionary history and ecological implications

BackgroundAmmonia-oxidizing archaea (AOA) of the phylum Thaumarchaeota mediate the rate-limiting step of nitrification and remove the ammonia that inhibits the aerobic metabolism of methanotrophs. However, the AOA that inhabit deep-sea methane-seep surface sediments (DMS) are rarely studied. Here, we used global DMS metagenomics and metagenome-assembled genomes (MAGs) to investigate the metabolic activity, evolutionary history, and ecological contributions of AOA. Expression of AOA-specific ammonia-oxidizing gene (amoA) was examined in the sediments collected from the South China Sea (SCS) to identify their active ammonia metabolism in the DMS.ResultsOur analysis indicated that AOA contribute > 75% to the composition of ammonia-utilization genes within the surface layers (above 30 cm) of global DMS. The AOA-specific ammonia-oxidizing gene was actively expressed in the DMS collected from the SCS. Phylogenomic analysis of medium-/high-quality MAGs from 18 DMS-AOA indicated that they evolved from ancestors in the barren deep-sea sediment and then expanded from the DMS to shallow water forming an amoA-NP-gamma clade-affiliated lineage. Molecular dating suggests that the DMS-AOA origination coincided with the Neoproterozoic oxidation event (NOE), which occurred ~ 800 million years ago (mya), and their expansion to shallow water coincided with the Sturtian glaciation (~ 713 mya). Comparative genomic analysis suggests that DMS-AOA exhibit higher requirement of carbon source for protein synthesis with enhanced genomic capability for osmotic regulation, motility, chemotaxis, and utilization of exogenous organic compounds, suggesting it could be more heterotrophic compared with other lineages.ConclusionOur findings provide new insights into the evolutionary history of AOA within the Thaumarchaeota, highlighting their critical roles in nitrogen cycling in the global DMS ecosystems.EWMb7UH3jhRcWd8tHNUGyqVideo

Gulf Stream mesoscale variabilities drive bottom marine heatwaves in Northwest Atlantic continental margin methane seeps

Methane hydrates on continental margins sequester substantial amounts of methane. Ocean warming often triggers hydrate dissociation, influencing global climate and marine ecosystems. Here we use ocean reanalysis to investigate bottom marine heatwaves affecting methane seeps at the Northwest Atlantic continental margin. These heatwaves are driven by downward displacement of ocean subsurface isopycnals that brings anomalously warm water to the seafloor, which frequently lacked surface expressions. The isopycnal anomalies are generated by the interaction of Gulf Stream mesoscale activity and slope topography, including warm core ring intrusions and impingements at the tail of the Grand Banks. Once generated, the isopycnal anomalies propagate along the slope and affect the seep area via topographic Rossby waves, offering predictability for bottom marine heatwaves. A northward shift and increasing instability of the Gulf Stream has intensified bottom marine heatwaves at the seep area since 2009, and future climate change will likely exacerbate this trend.

Microscale δ34S and δ18O variations of barite as an archive for fluid mixing and microbial sulphur metabolisms in igneous rock aquifers

ABSTRACT The stable isotope compositions of sulphur (δ34S) and oxygen (δ18O) in barite are frequently used as proxies for microbial sulphate reduction (MSR) in diverse environments, such as in relation to anaerobic oxidation of methane in marine cold seeps. There, isotopically heavy barite is used as a marker for MSR from a sulphate pool that has undergone semi-closed system conditions. Closed-system MSR is also a commonly observed feature in igneous rock hosted fracture aquifers, as shown by extremely 34S-enriched pyrite. What is less well-constrained is whether δ34S in barite can be used as a proxy for MSR in such systems. Here we explore the microscale heterogeneity of δ34S and δ18O via secondary ion mass spectrometry and the trace element Sr via LA-ICP-MS maps in barite precipitated in granite-hosted boreholes during a 17-year experiment, at Äspö, Sweden. We compare it with δ18Osulfate, δ34Ssulfate, and δ34Ssulfide of the fracture fluids and with paragenetic pyrite with δ34S values reflecting closed system MSR. The δ18O values in barite (+9.4 to +16.9 ‰) represent two generations of barite, one with low values and one with high values. The latter are likely impacted by sulphur disproportionating or -oxidizing bacteria. The barite reflects a much smaller span in δ34S (+14.5 to +28.6 ‰) than the pyrite (−47.2 to +53.3 ‰). This lack of extremely high δ34Sbarite values is proposed to be due to that barite saturation only occurred in the early parts of the Rayleigh cycle. Additionally, fluid migration has affected the δ34S values to lower values, accompanied by higher Sr concentrations. Taken together, barite δ34S values cannot be regarded as a reliable independent proxy for MSR in deep sulphate-poor igneous rock hosted aquifers. However, the relation between the δ34S values of coeval barite and pyrite is regarded as a useful proxy for MSR-related fractionation during early stages of MSR.

Cold seep nitrogen fixation and its potential relationship with sulfur cycling.

N2 fixation is an important source of biologically available in carbon-dominated cold seep systems as little nitrogen is released by hydrocarbon seepage, thereby promoting biological productivity and the degradation of non-nitrogenous organic matter. Cold seeps are rich in diverse sources of dissolved organic matter (DOM) derived from the sinking of photosynthetic products in euphotic layer and the release of chemosynthesis products on the seafloor. However, it remains unclear whether N2 fixation is coupled to the metabolic processes of DOM, as determined by e.g., carbon, nitrogen, phosphorus, and sulfur content, for energy acquisition in sulfur-rich cold seeps. In this study, diazotroph community structure and its response to DOM compositions were revealed. Moreover, the metagenomics analysis suggested that Dechloromonas genus plays a dominant role in potential coupling N2 fixation and sulfur oxidation. Our study highlighted that sulfur oxidation in deep-sea cold seeps may serve as an energy source to drive N2 fixation.

Experimental study on characteristics of gas seepage in broken coal and rock

AbstractThe characteristics of gas seepage in broken coal and rock composed of different particle sizes and grades were investigated in this study. On the basis of Darcy's law and non‐Darcy seepage theory, equations of gas permeability in the nonlinear seepage of broken coal and rock, as well as the porosity of broken coal and rock, under triaxial compression were determined. The stress loading path of gas seepage in broken coal and rock was developed. The characteristics of gas seepage in broken coal and rock composed of different particle sizes and grades were analyzed, and the results showed that the gas permeability after compression was proportional to the particle size of the broken coal and rock. Under triaxial compression, the gas permeability of the broken coal and rock composed of graded‐particle sizes was lower than that of the broken coal and rock composed of different single‐particle sizes. The gas permeability of the broken coal was lower than that of the broken rock mass, and the gas permeability and porosity of the broken coal and rock can be described by the exponential decay function. At a constant porosity, the gas permeability of the broken coal and rock was proportional to the size grading index under triaxial compression. The coefficient of viscosity and gravity of the flow are key factors influencing the flow permeability in broken coal and rock. This study provides a reference for on‐site practice such as the efficient extraction of gas in goafs.

Magnesium geochemistry of authigenic carbonate at marine cold seep

Cold seeps, featured by their extremely methane-rich sedimentary environments, play a significant role in the geological history and are common in marine sediments across the seafloor. Primary dolomite, possibly mediated by microorganisms, can be widely discovered in methane-rich environments. Hence, cold seeps may provide new insights into the ‘dolomite problem’, which has confused geologists for decades. Magnesium isotope geochemistry of seep carbonates contributes to the understanding of the dolomite formation mechanism in marine environments. In this paper, magnesium geochemical characteristics of carbonates in modern sediments are summarized, along with rare researches on magnesium isotopes of seep carbonates. Methane vigorously interacts with sulfate by anaerobic oxidation of methane at cold seeps, producing vast amounts of dissolved sulfide which can significantly promote dolomitization of seep carbonates. Compared with temperature, alkalinity, mineralogy, etc., the competition between rapid carbonate precipitation rates and aqueous ligands may be the main factor of the magnesium fractionation at cold seeps, which is controlled by the kinetic effect. The range of magnesium isotopes of seep carbonates is narrow (from -3.46‰ to -2.36‰), and an upper limit of magnesium content seems to exist. This characteristic may be a good indicator for identifying dolomitization related to anaerobic oxidation of methane. Whereas, mechanisms of magnesium isotope fractionation and dolomitization at cold seeps remain unclear, necessitating more natural samples tested, stimulated calculation and laboratory experiment.

Constraining the sources of archaeal tetraether lipids in multiple cold seep provinces of the Cascadia Margin

Archaeal isoprenoid glycerol dialkyl glycerol tetraether lipid (iGDGT) abundance profiles and carbon isotopic compositions reflect the relative distributions of archaeal sources, including planktonic, benthic, and methane-cycling contributions. Here, we analyze the carbon isotope ratios of iGDGTs purified from sediments of three different cold seep sites in Cascadia Margin, off the coast of Washington, USA. Together with relative abundance and glycerol configurations, we use the carbon isotope ratios to estimate the contributions of multiple archaeal sources to the sedimentary iGDGT assemblages and their impact on values of the TEX86 and methane indices. Using a Bayesian mixing model, we robustly characterize three potential endmembers by determining their characteristic lipid distributions, inferred contributions to the total sediment inventory, and carbon isotopic signatures. Despite the geographic proximity of the sample locations, we find site-specific heterogeneity in relative iGDGT abundances and δ13C values. Planktonic and benthic methane-cycling sources predominate in all cases (contributing > 98 % of iGDGTs), while benthic non-methane cycling archaea contribute minimally to the sedimentary lipid pool. Environments with higher methane influence show an increased presence of anti-parallel iGDGTs, indicating that methane-cycling archaea may dominantly or exclusively synthesize iGDGTs in this configuration. Our results quantify the relationship between the methane index (MI) and methane impact in systems dominated by planktonic and benthic methane-cycling archaea. Within the framework of the TEX86 temperature proxy, this permits a quantitative demonstration that it is overly simplistic to apply a MI cutoff threshold as a binary indicator to determine methane influence, and caution is needed when taking this approach in paleoclimate reconstructions.

Analysis of dominant flow in tectonic coal during coalbed methane transport

Diffusion and seepage are the main flow forms of coal seam gas transport, and are one of the key factors in the selection of gas extraction improvement methods. Changes in the physical structure of tectonic coal make gas transport more complex during coalbed methane extraction. In this paper, we develop a multi-field coupled model of methane transport in coal seams, taking into account the effects of tectonics, and theoretically analyze the dominant flow patterns for methane extraction. Then, the evolution of gas dominated flow is analyzed for different initial pressures, initial permeabilities, and initial diffusion coefficients of tectonic and intact coal seams. The results show that the amount of daily methane seepage in tectonic coal increases with the initial pressure of the coal reservoir, but decreases with the initial diffusion coefficient of the coal reservoir. Methane seepage in tectonic coal has a longer control time than in intact coal at different initial pressures, initial permeabilities, and initial diffusion coefficients of the coal reservoir. For different coal reservoir initial pressures, coal reservoir initial permeabilities, and coal reservoir initial diffusion coefficients, the maximum seepage control time for tectonic coal is 20, 17, and 15 times longer than for intact coal, respectively. Finally, the discrepancies of methane dominant flow in tectonic coal and intact coal during methane extraction were analyzed by using the double bottleneck flow model, and methods for methane enhanced extraction in tectonic coal and intact coal were discussed. The results presented in this paper may provide a theoretical reference for the extraction of differentiated gas in coal seams.

Numerical Investigation of coupling hazard zone of coal spontaneous combustion and gas in gob for high-gas mines

As coal mining operations continue to deepen, the frequency of coal spontaneous combustion (CSC) and gas explosion disasters in high-gas mines is increasing. Understanding the distribution patterns of CSC and gas compound disaster zones in the gob during the extraction process is crucial for reducing the risks of combustion and explosion. This study focuses on the 201 working face of the Shuanglong Coal Mine, where a multi-physical field coupling model of pressure, temperature, gas seepage, and diffusion was established to investigate the evolution characteristics of compound disaster zones and propose corresponding prevention and control measures. The results demonstrate that the area with the most severe composite hazard is mainly located at the return roadway side, extending to the middle of the gob. With increased ventilation volume and advance rate, the area of the CSC and gas coupling hazard zone expands continuously. By implementing measures such as brattice cloth and nitrogen injection, the areas of spontaneous combustion and CH4 explosion zones were reduced by 53.6 % and 49.5 %, respectively, effectively controlling the extent of the compound disaster zone and eliminating the risk of gas explosions.

Biogeochemical Reconstruction of Authigenic Carbonate Deposits at Methane Seep Site off Krishna‐Godavari (K‐G) Basin, Bay of Bengal

AbstractActive and relic marine methane‐seep sites are widely distributed globally and are distinguished by distinctive geology, biogeochemistry, and ecosystems. The discovery of methane‐seep sites in the Krishna‐Godavari (K‐G) basin has created exciting new opportunities for methane‐seep research in the Bay of Bengal. In this study, we document the occurrence of authigenic carbonates, including micro‐crystalline aragonite crust (arg‐crusts) admixed with chemosynthetic shells and high‐magnesium carbonate tubular structures (HMC‐tube), from the methane‐seep site SSD‐045/4 in the K‐G basin. The δ13C values of HMC‐tubes (−54.5 to −46.2‰) and arg‐crusts (−57.6 to −34.8‰) indicate biogenic methane as the likely carbon source. Enhanced porewater alkalinity driving carbonate precipitation may be attributed to microbial‐mediated SO₄2−‐AOM processes. Additionally, δ13C values (−35.2 ± 8‰) of the residual organic matter within the carbonates suggest a contribution of methanotrophic bacterial biomass. The δ18Ocarb values of HMC and aragonite indicate methane hydrate degassing and crystallization pathways, respectively. Pelloid‐filled burrows suggest the reworking of shallow HMC deposit by burrowing organisms, whereas the polyphase cementations (aragonite and HMC) within burrows indicate early and burial diagenetic pathways. The wide range in ΣLREE/ΣHREE ratios and Ceanom values in arg‐crusts reflect micro‐spatial variations in redox conditions, likely due to cementation occurring in both open and closed diagenetic systems. In contrast, more constrained Ceanom values and ΣLREE/ΣHREE ratios in HMC tubes suggest persistent sulfidic conditions. Overall, these findings provide insights into the pathways of carbonate formation at the K‐G basin methane‐seep site, highlighting the complex interplay of microbial processes, fluid dynamics, and diagenetic alterations.

Hydrocarbon-degrading microbial populations in permanently cold deep-sea sediments in the NW Atlantic

Permanently cold deep-sea sediments (2500–3500 m water depth) with and without indications of thermogenic hydrocarbon seepage were exposed to naphtha to examine the presence and potential of cold-adapted aerobic hydrocarbon-degrading microbial populations. Monitoring these microcosms for volatile hydrocarbons by GC–MS revealed sediments without in situ hydrocarbons responded more rapidly to naphtha amendment than hydrocarbon seep sediments overall, but seep sediments removed aromatic hydrocarbons benzene, toluene, ethylbenzene and xylene (BTEX) more readily. Naphtha-driven aerobic respiration was more evident in surface sediment (0–20 cmbsf) than deeper anoxic layers (>130 cmbsf) that responded less rapidly. In all cases, enrichment of Gammaproteobacteria included lineages of Oleispira, Pseudomonas, and Alteromonas known to be associated with marine oil spills. On the other hand, taxa known to be prevalent in situ and diagnostic for thermogenic hydrocarbon seepage in deep sea sediment, did not respond to naphtha amendment. This suggests a limited role for these prevalent seep-associated populations in the context of aerobic hydrocarbon biodegradation.

Olfactory-based powered descent guidance for Mars methane plume exploration in long-time-average wind environment

This paper investigates an olfactory-based powered descent guidance method that enable a lander to autonomously locate and target the vent source of any methane plume in long-time-average wind environment on Mars. The episodic methane plumes emanating from the Martian surface invite the possibility of direct access to the subsurface methane reservoir to acquire pristine organic materials. Constrained by the insufficient knowledge of the accurate plume vent location, Mars landers must infer the target location — the plume source — autonomously during the powered descent. However, existing powered descent guidance methods require the target location as a terminal constraint, rendering them ineffective in plume exploration missions. We propose an olfactory-based powered descent guidance method that could steer a lander to target the methane vent source by tracking the gradient of methane concentration in long-time-average wind environment, imposing sub-optimal fuel consumption. A novel olfactory navigation method based on tracking the negative logarithmic concentration gradient, and the corresponding gradient measurement method, is proposed. By implementing the negative logarithmic gradient field, gradient-dependent dynamic equations for the powered descending lander are proposed. The gradient-dependent equations provide a way to transform the original optimal guidance problem, which is non-convex and terminal-free, into a convex and terminal-fixed problem. A suboptimal guidance law, similar to E-guidance, is then derived by solving this problem. The proposed guidance could target the vent source of any methane plume in long-time-average wind environment, imposing modest fuel consumption by feeding back the concentration gradient. Numerical simulations illustrate that, in comparison to the terminal-fixed E-guidance and the open-loop fuel-optimal guidance, the lander imposes additional fuel consumption of 2.57% and 12.15% respectively, using the proposed guidance law.

Variations in deep-sea methane seepage linked to millennial-scale changes in bottom water temperatures ~ 50–6 ka, NW Svalbard margin

During the last glaciation, the northern hemisphere experienced profound millennial-scale changes (termed Dansgaard-Oeschger (DO) events) in atmospheric and oceanic temperatures. In the North Atlantic, the fluctuations resulted in extremely unstable bottom water conditions with bottom water temperatures (BWT) varying up to > 5 °C. We have studied these changes in a core from 1,300 m water depth at Vestnesa Ridge, northwestern Svalbard margin to investigate a possible connection between BWT and seepage of methane from the seafloor covering the period ~ 50–6 ka. Beneath Vestnesa Ridge, gas hydrates containing vast amounts of methane are kept stable due to the high pressure and low temperatures. Release of gas is shown by numerous pockmarks on the seafloor. The pockmarks at 1,300 m water depth are presently inactive, but they bear witness of earlier activity. Our study shows that from ~ 50–6 ka, the core site experienced repeated increases in BWT and in the emissions of gas, both following the pattern of the DO events. This correspondence in time scale indicates that BWT was the primary forcing factor for the variability in methane release. However, the releases were delayed by up to > 1,000 years compared to the initial increase in BWT.

Investigation on microscopic pore structure optimization and Lattice Boltzmann seepage law in coal modified by N-methylpyrrolidone composite solvents

This study evaluated the impact of composite solvents (1-butyl-3-methylimidazolium tetrafluoroborate [Bmim]BF4, sodium dodecyl sulfate (SDS), and N-methyl pyrrolidone (NMP)) on microscale pore-fracture structures and mesoscopic gas seepage in coal samples; by employing fractal theory and the Lattice Boltzmann method (LBM). Under combined application of three composite solvents: (1) there was a substantial 23.66 % increase in the scanning electron microscopy fractal dimension of the samples, which signifies enhanced complexity and nonhomogeneity of the samples; (2) the percentages of mesopores and macropores in the samples experienced a 10.64 % increase, resulting in a 20.45 % increase in overall porosity; (3) the effective pore fractal dimension (Da) and seepage pore fractal dimension (Ds) exhibited respective decreases of 3.05 % and 0.57 %; and (4) the average gas seepage velocity substantially increased by 53.57 %, with the optimal flow channel development position along the x-direction being closer to the exit (x = 170). These phenomena were attributed to the following: (1) introducing SDS reagents enhanced coal hydrophilicity; (2) NMP and [Bmim]BF4 aqueous solvents could more easily infiltrate the minuscule pores; (3) the synergy of the composite solvents disrupted the pore-fracture structures of the coal matrix, leading to a substantial increase in the aperture and area of the open pores; and (4) this process enhanced the connectivity, consequently increasing the average seepage velocity.

Dynamic gas emission during coal seam drilling under the thermo-hydro-mechanical coupling effect: A theoretical model and numerical simulations

The potential risk of coal and gas outbursts has increased with the depth of coal mining, posing the threat of heavy casualties, vast economic losses, and harm to the ecological environment. Considering the limitations of existing coal and gas outburst prediction methods, novel indicators or methods are needed. In this study, a fully coupled dynamic drilling gas emission THM model of gas diffusion, seepage, and flow is established, and the reliability of the model was verified by the measured data. The validated model was applied to single-factor and multi-factor impact analysis of dynamic drilling gas emission. The simulation results showed that (1) During the drilling process, the increase in permeability is mainly attributed to the coal seam damage and disturbance caused by the borehole and the coupling effects of the THM model, which increase the porosity around the borehole. Driven by the pressure gradient and temperature gradient, the gas pressure and temperature of the coal seam around the borehole show a conical distribution. (2) In the single-factor analysis, increasing the values of coal seam parameters (initial gas pressure, initial permeability, and initial coal temperature) and drilling parameters (borehole radius) will promote gas emissions in the drilling process to different degrees. The most significant promoting effect is the initial permeability, and the weakest is the initial coal temperature. (3) The response surface analysis results of the four factors show that the interaction between initial coal temperature C and borehole radius D is the least significant, and the interaction between initial gas pressure A and initial permeability B is the most significant. Meanwhile, the established multiple regression model characterizes the relationship between borehole gas emissions and initial coal seam gas pressure, and the idea of using drilling gas emission volume to predict coal and gas outbursts was proposed.

Micro–Nano 3D CT Scanning to Assess the Impact of Microparameters of Volcanic Reservoirs on Gas Migration

Volcanic rock reservoirs for oil and gas are known worldwide for their considerable heterogeneity. Micropores and fractures play vital roles in the storage and transportation of natural gas. Samples from volcanic reservoirs in Songliao Basin, CS1 and W21, belonging to the Changling fault depression and the Wangfu fault depression, respectively, have similar lithology. This study employs micro–nano CT scanning technology to systematically identify the key parameters and transport capacities of natural gas within volcanic reservoirs. Using Avizo 2020.1software, a 3D digital representation of rock core was reconstructed to model pore distribution, connectivity, pore–throat networks, and fractures. These models are then analyzed to evaluate pore/throat structures and fractures alongside microscopic parameters. The relationship between micropore–throat structure parameters and permeability was investigated by microscale gas flow simulations and Pearson correlation analyses. The results showed that the CS1 sample significantly exceeded the W21 sample in terms of pore connectivity and permeability, with connected pore volume, throat count, and specific surface area being more than double that of the W21 sample. Pore–throat parameters are decisive for natural gas storage and transport. Additionally, based on seepage simulation and the pore–throat model, the specific influence of pore–throat structure parameters on permeability in volcanic reservoirs was quantified. In areas with well–developed fractures, gas seepage pathways mainly follow fractures, significantly improving gas flow efficiency. In areas with fewer fractures, throat radius has the most significant impact on permeability, followed by pore radius and throat length.

Thickness and Structure of Permafrost in Oil and Gas Fields of the Yamal Peninsula: Evidence from Shallow Transient Electromagnetic (sTEM) Survey

The Yamal-Nenets Autonomous District, especially the Yamal Peninsula located in the permafrost zone, stores Russia’s largest oil and gas resources. However, development in the area is challenging because of its harsh climate and engineering–geological features. Drilling in oil and gas fields in permafrost faces problems that are fraught with serious accident risks: soil heaving leading to the collapse of wellheads and hole walls, deformation and breakage of casing strings, gas seeps or explosive emissions, etc. In this respect, knowledge of the permafrost’s structure is indispensable to ensure safe geological exploration and petroleum production in high-latitude regions. The extent and structure of permafrost in West Siberia, especially in its northern part (Yamal and Gydan Peninsulas), remain poorly studied. More insights into the permafrost’s structure have been obtained by a precise sTEM survey in the northern Yamal Peninsula. The sTEM soundings were performed in a large oil and gas field where permafrost is subject to natural and anthropogenic impacts, and its degradation, with freezing–thawing fluctuations and frost deformation, poses risks to exploration and development operations, as well as to production infrastructure. The results show that permafrost in the western part of the Yamal geocryological province is continuous laterally but encloses subriver and sublake unfrozen zones (taliks) and lenses of saline liquid material (cryopegs). The total thickness of perennially frozen rocks is 200 m. The rocks below 200 m have negative temperatures but are free from pore ice. Conductive features (<10 Ohm﮲m) traceable to the permafrost base may represent faults that act as pathways for water and gas fluids and, thus, can cause a geohazard in the oil and gas fields (explosion of frost mounds, gas blow during shallow drilling, etc.).

FMAW-YOLOv5s: A deep learning method for detection of methane plumes using optical images

Natural gas hydrates stored in the subsurface seabed of continental margins are one of the most important carbon reservoirs on Earth. Research on natural gas hydrates is of great significance to global warming and ecological protection. Methane plumes caused by crustal dynamics are usually considered as a sign of existence of natural gas hydrates. Detection of methane plumes thus becomes the first step of cold seep research. This paper conducts comprehensive research on detection of methane plumes based on deep learning methods and optical images. First, we proposed a method of creating high quality and balanced datasets for methane plumes detection tasks using open-source videos. We then proposed a FMAW-YOLOv5s method for methane plumes detection. The FMAW-YOLOv5s method improves the traditional YOLOv5s in design of backbone network, neck network and loss function. The FMAW-YOLOv5s method can realize accurate and fast detection of methane plumes with a precision of 96.9% and FPS of 141.7. The lightweight feature of FMAW-YOLOv5s also enables the deployment in edge computing devices such as AUVs and ROVs. This research can not only promote the study of cold seep activities, but also provide meaningful insights for detection of other underwater events such as gas pipelines leakage.

Insights into prokaryotic communities and their potential functions in biogeochemical cycles in cold seep.

Deep-sea cold seeps are among the most productive ecosystems, sustaining unique fauna and microbial communities through the release of methane and other hydrocarbons. Our study revealed that the influence of seepage fluid on the prokaryotic community in the water column is surprisingly limited, which challenges conventional views regarding the impact of seepage fluids. In addition, we identified that different DOM compositions play a crucial role in shaping the prokaryotic community composition, providing new insights into the factors driving microbial diversity in cold seeps. Furthermore, the study highlighted Proteobacteria as key and multifaceted drivers of biogeochemical cycles in cold seeps, emphasizing their significant contribution to complex interactions and processes. These findings offer a fresh perspective on the dynamics of cold-seep environments and their microbial communities, advancing our understanding of the biogeochemical functions in deep-sea environments.

Chemolithoautotrophic bacteria flourish at dark water-ice interfaces of an emerged Arctic cold seep.

Below their ice shells, icy moons may offer a source of chemical energy that could support microbial life in the absence of light. In the Arctic, past and present glacial retreat leads to isostatic uplift of sediments through which cold and methane-saturated groundwater travels. This fluid reaches the surface and freezes as hill-shaped icings during winter, producing dark ice-water interfaces above water ponds containing chemical energy sources. In one such system characterized by elevated methane concentrations - the Lagoon Pingo in Adventdalen, Svalbard (~10mg/L CH4, <0.3mg/L O2, -0.25°C, pH7.9), we studied amplicons of the bacterial and archaeal (microbial) 16S rRNA gene and transcripts in the water pond and overlaying ice. We show that active chemolithoautotrophic sulfur-oxidizing microorganisms (Sulfurimonas, Thiomicrorhabdus) dominated a niche at the bottom of the ice in contact with the anoxic water reservoir. There, the growing ice offers surfaces interfacing with water, and hosts favorable physico-chemical conditions for sulfide oxidation. Detection of anaerobic methanotrophs further suggests that the ice led to a steady-state dark and cold methane sink under the ice throughout winter, in two steps: first methane is oxidized to carbon dioxide and sulfates concomitantly reduced to sulfides by the activity of ANME-1a and SEEP-SRB1 consortia, in a second time energy from sulfides is used by sulfur- oxidizing microorganisms to fix carbon dioxide into organic carbon. Our results underline ice- covered and dark ecosystems as a hitherto overlooked oasis of microbial life and emphasize the need to study microbial communities in icy habitats.