Gas Chromatography-mass Spectrometry Research Articles

Antimicrobial Activity of Silver Nanoparticles Against Common Bovine Mastitis Pathogens: A Comparative Analysis.

Bovine mastitis is one important metabolic disease in dairy cows caused by S. aureus and E. coli impacting huge economic losses worldwide. Silver Nanoparticles (AgNPs) possess unique electrical, thermal, optical, and biological properties, scrutinizing them as more effective antimicrobial agents. This study aimed to synthesize and evaluate AgNPs as effective antimicrobial compounds against S. aureus and E. coli. In the present work, the extract of citrus limon was analysed with gas chromatography-mass spectrometry (GC-MS) analysis, citric acid was found abundant in methanolic solvent. Synthesis of AgNPs was made by both green and chemical synthesis method, charctererized by UV‒visible spectroscopy, electron microscopy, zeta sizing and infrared spectroscopy. The result confirmed that both green and chemical AgNPs are in nanoscale range with average size ranged from 10 to 20nm. The zeta potential was found negative and green synthesized AgNPs resulted significantly (P ≤ 0.05) higher antimicrobial activities than chemical synthesized AgNPs and also commercial available antibiotics. The minimum inhibitory concentration (MIC) was also evaluated and green synthesized AgNPs resulted MIC50 value of 46.10 and 49.93 while chemical synthesized AgNPs showed MIC50 value of 77.39 and 86.50μgmL-1 against S. aureus and E. coli respectively. Additionally, green synthesized AgNPs demonstrated more cell viability (%) against RAW264.7 cells. Therefore, green AgNPs would be a potential antimicrobial agent in place commercially available antibiotics against aforesaid organisms in nearest future.

Allelochemicals degradation and multifarious plant growth promoting potential of two Bacillus spp.: Insights into genomic potential and abiotic stress alleviation.

The deposition of allelochemicals poses a challenge to continuous cropping. Microbial degradation is an efficient approach to degrade these hazardous compounds. The current study employed an integrated approach to explore the allelochemical degradation potential of Bacillus subtilis RS10 and Bacillus pumilus SF-4 and concurrently validate their capabilities to enhance plant growth and alleviate abiotic stress in pot experiments. During initial in vitro screening, both strains utilized more than 45% of benzoic acid within 60h of incubation and showed maximum growth after 72h. Meanwhile, the wheat seed germination rate was increased by 34.33% and 30% when treated with strain RS10 and SF-4, respectively. In addition, both strains demonstrated the capacity to promote wheat growth in terms of root length, shoot length, and plant weight in soil contaminated with p-hydroxybenzoic acid. To determine the associated mechanism of plant growth-promoting and allelochemical degradation, the culture extract of RS10 and SF-4 were analyzed using gas chromatography-mass spectrometry, which showed several plant growth-promoting volatile organic compounds, including propanediol and butanone. Genome-wide analysis unveiled several genetic loci associated with plant growth-promoting traits such as siderophore synthesis, phosphate solubilization, and biosynthesis of biocontrol compounds. Moreover, the in-depth comparative genome analysis, horizontal gene transfer, and strain-specific genes unveiled intriguing insight into the evolutionary dynamics of these strains and constraints driven by natural selection. In conclusion, the current study revealed the multifarious plant growth-promoting traits of strains RS10 and SF-4 and suggested an application of these strains as plant growth stimulators in soil contaminated with allelochemicals.

Photodegradation process and mechanism of 2,3,6-trichloronaphthalene on kaolinite surfaces under ultraviolet-A irradiation: Role of fulvic acid and density functional theory calculations.

Polychlorinated naphthalenes (PCNs), a class of persistent organic pollutants (POPs), pose significant environmental and health risks, with trichloronaphthalene being a predominant congener in atmospheric particulate matter. This study investigates the photodegradation of 2,3,6-trichloronaphthalene (CN-26) on kaolinite surfaces under ultraviolet-A (UV-A) irradiation, focusing on the impact of fulvic acid (FA), temperature, humidity, and pH. The photodegradation mechanism of CN-26 was inferred via radical quenching experiments and density functional theory (DFT) calculations. The optimized degradation rate of CN-26 was 75.57 % at 25 °C, 70 % humidity, and pH 7 when FA was added at a concentration of 30 mg kg-1. Based on the radical quenching experiments, •OH are the primary active species involved in the degradation of CN-26, followed by electrons. In the absence of FA, •OH contributed 82.21 %, while electronic was 17.79 %. Conversely, in the presence of FA, the contribution rates of •OH, and electronic are 68.32 % and 21.21 % respectively. DFT calculations indicated that the 6 C site of CN-26 exhibited the highest susceptibility to radical attack, with the highest FED2HOMO+FED2LUMO value (0.25273), corroborated by averaged local ionization energy (ALIE) analysis. In the analysis of the reaction of •OH with CN-26, the lowest transition state ΔrG value of 1.09 kcal mol-1 was observed for compound 6 C, indicating that this site is the most susceptible to •OH attack. The degradation products of CN-26 were detected using gas chromatography-mass spectrometry (GC-MS), and the possible photodegradation pathways were proposed, which included dechlorination, hydroxylation, and aromatic ring opening. This study would provide insights into the photochemical behaviors of PCNs.

Metabolic profiling reveals distinctive ripening dynamics in ethylene-treatedMusa balbisiana cv. 'Pisang Klutuk Wulung' comparedtocommercial Cavendish banana.

As an important crop, bananas still encounter fruit quality and shelf-life problems that are affected by the ripening process. Improving postharvest technologies may effectively address these challenges, such as by studying the ripening mechanism of banana cultivars with a slow ripening process. A banana cultivar that exhibits this characteristic is Musa balbisiana cv. 'Pisang Klutuk Wulung' (BB Group) or Pisang Klutuk Wulung (PKW), which has a ripening duration of 14-28 days. However, the metabolomics study on the ripening mechanism of this banana is still limited. This study aimed to analyze metabolite changes in ethylene-treated Pisang Klutuk Wulung during ripening in comparison to commercial bananas (Cavendish). Both bananas were subjected to exogenous ethylene treatment and analyzed using gas chromatography-mass spectrometry to perform metabolite profiling throughout the ripening process. The principal component analysis showed sample separation based on the ripening stages and banana species in pulp and peel. Orthogonal projection to latent structure analysis suggested that metabolite changes accompanied the ripening stages. Potential metabolite markers that distinguished the ripening of PKW and Cavendish were found, such as quinic acid, inositol, and 2-aminoethanol. This study shows differences in metabolite profiles between these bananas, especially the metabolites involved in sugar metabolism, cell wall metabolism, stress response, and biosynthesis of aromatic compounds. This study provides novel insights into the metabolic changes occurring during PKW ripening, contributing to the improvement of banana postharvest strategies.

Salicylic Acid Modulates Volatile Organic Compound Profiles During CEVd Infection in Tomato Plants

Background:Citrus Exocortis Viroid (CEVd) is a non-coding RNA pathogen capable of infecting a wide range of plant species, despite its lack of protein-coding ability. Viroid infections induce significant alterations in various physiological and biochemical processes, particularly impacting plant metabolism. This study shows the metabolic changes upon viroid infection in tomato plants (Solanum lycopersicum var. ‘MoneyMaker’) exhibiting altered levels of salicylic acid (SA), a key signal molecule involved in the plant defence against this pathogen. Methods: Transgenic RNAi_S5H lines, which have the salicylic acid 5-hydroxylase gene silenced to promote SA accumulation, and NahG lines, which overexpress a salicylate hydroxylase to degrade SA into catechol and prevent its accumulation, were used to establish different SA levels in plants, resulting in varying degrees of resistance to viroid infection. The analysis was performed by using gas chromatography–mass spectrometry (GC-MS) to explore the role of volatile organic compounds (VOCs) in plant immunity against this pathogen. Results: Our results revealed distinct volatile profiles associated with plant immunity, where RNAi_S5H-resistant plants showed significantly enhanced production of monoterpenoids upon viroid infection. Moreover, viroid-susceptible NahG plants emitted a broad range of VOCs, whilst viroid-tolerant RNAi_S5H plants exhibited less variation in VOC emission. Conclusions: This study demonstrates that SA levels significantly influence metabolic responses and immunity in tomato plants infected by CEVd. The identification of differential emitted VOCs upon CEVd infection could allow the development of biomarkers for disease or strategies for disease control.

In vitro anticancer effects of frankincense and its nanoemulsions for enhanced cancer cell targeting

IntroductionFrankincense has demonstrated promising in vitro anticancer activity. However, its conventional delivery methods face significant challenges due to limited oral bioavailability. To address these limitations, this study focuses on developing optimized nanoemulsions (NEs) of Frankincense oil (FO) to enhance its therapeutic efficacy.MethodsFrankincense resins were extracted and characterized using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), identifying key metabolites including isopinocarveol, α-thujene, p-cymene, carvone, germacrene A, and various methyl esters. FO-based nanoemulsions (FO-NEs) were prepared and optimized using a 3-factor, 3-level Box-Behnken Design (BBD), with 10% FO (v/v), 40% surfactant (cremophor EL), and co-surfactant (Transcutol P). The optimized FO-NEs were evaluated for particle size, polydispersity index (PDI), zeta potential, and morphology using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cytotoxicity, wound healing, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) assays were performed against breast cancer (MDA-MB-231, MDA-MB-231-TR) and lung cancer (A549, A549-TR, H1299) cell lines.ResultsThe optimized FO-NEs exhibited an average particle size of 65.1 ± 4.21 nm, a PDI of 0.258 ± 0.04, and a zeta potential of −22.3 ± 1.2 mV. SEM and AFM confirmed the spherical morphology of the FO-NEs. In vitro cytotoxicity studies revealed enhanced anticancer activity of FO-NEs (IC50 = 13.2 μg/mL) compared to free FO (IC50 = 22.5 μg/mL) against resistant breast cancer MDA-MB-231-TR cells. FO-NEs significantly improved cancer cell internalization, disrupted mitochondrial membrane potential, and increased ROS generation, leading to enhanced cytotoxic effects.DiscussionThe results demonstrate that nanoemulsion-based delivery significantly enhances the bioactivity and cellular uptake of frankincense oil compared to its free form. FO-NEs exhibit potent anticancer activity, particularly against drug-resistant cancer cell lines, suggesting their potential as a viable strategy for improving the therapeutic efficacy of frankincense in cancer treatment.

Prioritizing Chemical Features in Non-targeted Analysis through Spatial Trend Analysis: Application to the Identification of Organic Chemicals Subject to Mountain Cold-Trapping.

One of the challenges arising during non-targeted analysis (NTA) is that the number of detected chemical features is generally too large for detailed processing and interpretation. Here, we illustrate how the analysis of spatial trends in peak intensities can be an effective tool to prioritize chemical features in NTA. Specifically, features detected by gas chromatography and high-resolution mass spectrometry in soil and air samples, collected along an altitudinal transect on an urban mountain in Canada, were successfully grouped into different categories based on spatial trends with site altitude. The motivation was to identify features whose abundance increases in soil with increasing elevation, as the ability for amplification at higher elevations could characterize contaminants of concern to mountain ecosystems. Potential matching candidates were first selected by comparing empirically detected accurate masses and isotope distributions of chemical features with those in chemical databases. These potential candidates were then ranked by comparing MSMS spectra with fragments predicted in silico. Several highly ranked matches, as well as structurally related compounds, which were largely halogenated methoxylated benzenes and organochlorine pesticides, were then subjected to targeted analysis with analytical standards. Several of these compounds, including pentachloroanisole, tricamba, and 3,4,5-trichloroveratrole, were identified as having spatial patterns consistent with mountain cold-trapping, as evidenced by organic carbon-normalized soil concentrations that show a significant increase with elevation. Our study clearly demonstrated that spatial trend analysis holds considerable promise as a tool to guide chemical identification and prioritization during NTA.

Comprehensive two-dimensional gas chromatography–mass spectrometry

Comprehensive two-dimensional gas chromatography–mass spectrometry

Electron Beam Irradiation-Induced Defects Enhance Pt-TiO2 Photothermal Catalytic Degradation in PAEs: A Performance and Mechanism Study

Phthalic acid esters (PAEs), ubiquitous semi-volatile organic compounds (SVOCs) in indoor environments, pose adverse effects on human health. However, their degradation mechanisms and pathways remain unclear. Herein, we developed an efficient photothermal catalyst by introducing defects (oxygen vacancies, OVs) on TiO2 (P25) surfaces via electron beam irradiation technology with different irradiation doses (100, 300, 500, and 700 kGy). The TiO2 with defects was employed as a support to prepare Pt-TiO2 catalysts for the photothermal degradation of di (2-ethylhexyl) phthalate (DEMP) and dimethyl phthalate (DMP), two representative PAEs. TiO2 pre-treated with a 300 kGy irradiation dose supported the Pt catalyst (Pt-Ti-P-300) and presented the optimal catalytic performance for DEMP and DMP degradation. Characterization results confirmed that OVs were successfully introduced to the catalysts. Meanwhile, OVs induced by electron beam irradiation expanded the light absorption range and improved the generation and separation of photogenerated carriers, which significantly enhanced the catalytic activity of the catalysts for PAE degradation. Importantly, the degradation mechanism and pathway of DMP were further explored by using in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and gas chromatography–mass spectrometry (GC-MS). These findings provide important insights into the electron beam irradiation-mediated regulation of catalysts and the photothermal catalytic removal of PAEs in indoor environments.

Comparative Metabolic Profiling and Biological Evaluation of Essential Oils from Conocarpus Species: Antidiabetic, Antioxidant, and Antimicrobial Potential

Essential oils (EOs) are a diverse source of bioactive compounds with remarkable therapeutic potential. Despite their significance, Conocarpus EOs have been largely underexplored. This study provides a novel comparison of the metabolic profiles and biological activities of EOs from C. lancifolius, C. erectus green, and C. erectus silver leaves cultivated in the United Arab Emirates (UAE), offering unique insights into their distinct bioactive properties and potential therapeutic applications. EOs were extracted via hydro-distillation, analyzed using gas chromatography–mass spectrometry (GC-MS), and subjected to chemometric analysis. Their antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma (FRAP) assays), antidiabetic (α-amylase and α-glucosidase inhibition), acetylcholinesterase (AChE) inhibition and antimicrobial activities were assessed. A total of 92 metabolites were identified, with heptacosane and nonacosane as key species discriminants. C. lancifolius EO showed the strongest α-amylase (IC50 8.75 ± 0.54 µg/mL) and α-glucosidase (IC50 22.31 ± 0.92 µg/mL) inhibitory activities, while C. erectus silver demonstrated superior antioxidant capacity (IC50 349.78 ± 8.26 µg/mL, DPPH assay). C. lancifolius EO exhibited the best antimicrobial activity, particularly against Staphylococcus aureus (MIC 625 µg/mL). C. erectus silver EO inhibited E. coli and C. albicans (MIC 625 µg/mL). In contrast, C. erectus EOs showed no activity against Aspergillus niger. These findings highlight the potential of Conocarpus EOs as antioxidants and for managing diabetes that may be utilized either in nutraceuticals, dietary supplements or even in pharmaceutical formulations. Moreover, owing to significant antimicrobial activities, the EOs may be added to medical disinfectants and several pharmaceutical products. However, further, in vivo validation and pharmaceutical exploration is still needed.

Phenotypic plasticity vs. local genetic adaptation: essential oil diversity of natural immortelle (Helichrysum italicum (Roth.) G.Don) populations along eastern Adriatic coast

The essential oil of Helichrysum italicum (Roth) G.Don, commonly known as immortelle, is produced in Mediterranean countries to meet the increasing demand of the cosmetic and pharmaceutical industries. This study focused on the analysis of secondary metabolites, specifically essential oils, extracted from plants grown from the seeds of natural immortelle populations collected along the eastern Adriatic coast and cultivated ex situ under uniform conditions. Field trials were conducted to determine whether the observed variability was due to phenotypic plasticity or local genetic adaptation. Eighteen natural immortelle populations were sampled, hydrodistilled and their essential oil composition determined by gas chromatography-mass spectrometry. A total of 84 compounds were identified. Eighteen essential oil compounds were present in concentrations greater than 5% in at least one sample of 18 populations. The populations differed significantly in nine essential oil compounds: Limonene, linalool, nerol, neryl acetate, trans-caryophyllene, neryl propionate, ar-curcumene, β-selinene and δ-selinene and the differences were attributed to genetic adaptation to the native environment. Three chemotypes were identified within which the populations were grouped. Results showed a significant and strong correlation between biochemical and bioclimatic distance, with 22.4% of biochemical differentiation between populations explained by bioclimatic distance. Correlations between the 18 main compounds and the bioclimatic variables of the populations’ native environment revealed that BIO14 Precipitation of driest month and BIO15 Precipitation seasonality, were the most informative. These results can serve as a first step for future selection of immortelle populations with desirable adaptations to obtain commercial cultivars that ensure high quality immortelle essential oil.

Unraveling the levels of emerging contaminants along the eastern Mediterranean Sea

The Eastern Mediterranean Sea, rich in environmental and cultural heritage, faces increasing threats from emerging contaminants like toxic metals and phthalates. This study evaluates their occurrence across 40 Lebanese Mediterranean coastal hotspots using advanced techniques such as Inductively Coupled Plasma Mass Spectrometry (ICP-MS), X-ray Fluorescence Spectroscopy (XRF) and Gas Chromatography-Mass Spectrometry (GC–MS). Widespread contamination was detected in both water and sediments near landfills, sewage discharges, and industrial areas. Metal concentrations varied, with chromium (Cr) reaching 20.3 µg/L, arsenic (As) 12.1 µg/L, and lead (Pb) 30.6 µg/L, indicating pollution from urban and industrial activities. Manganese (Mn) and zinc (Zn) were within safe limits, while selenium (Se) and arsenic posed ecological risks. Among 13 phthalates, diethylhexyl phthalate (DEHP) was the most prevalent, ranging from 15.57 to 72.88 µg/L. Sediments showed elevated calcium, strontium, and barium levels, exceeding safety thresholds. Statistical analysis revealed correlations between contaminants and spatial variability driven by industrial, agricultural, and urban activities. These findings highlight the need for proper regulations and routine monitoring to protect marine ecosystems and public health.

Occurrence, Transport, and Risk Assessment of Brominated Flame Retardants in Northern Wetland Multimedia

Current studies have paid extensive attention to the occurrence of brominated flame retardants (BFRs) in aquatic environments; however, there is a lack of exploration of BFRs in ice media in freshwater environments, and there are fewer studies on the distribution patterns and ecological risks of BFRs in different media. In order to fill this gap in the current research status, this study conducted four seasonal samplings in the Songhua River wetland in Northeast China. The distribution and risk of 14 polybrominated diphenyl ethers (PBDEs) and 22 new brominated flame retardants (NBFRs) in water, ice, sediment, and soil were analyzed using liquid–liquid extraction sample pretreatment and gas chromatography–mass spectrometry instrumentation. A total of 18, 5, 8, 19, and 18 BFRs were detected in non-ice-covered water, ice-covered water, ice, sediment, and soil, respectively. NBFRs dominated contaminant concentrations in each medium. Significant correlations were found between BFRs in ice and subglacial water, suggesting that the sources of BFRs in these two media are similar and there is an exchange between them. The ice enrichment factor (IEF) revealed the water–ice distribution mechanism of BFRs, indicating that wetland ice acts as a temporary sink for 2-(Allyloxy)-1,3,5-tribromobenzene (ATE), 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane (α-TBECH), 1,2,5,6-Tetrabromocyclooctane (TBCO), and 2-Bromoallyl 2,4,6-tribromophenyl ether (BATE). In order to achieve dynamic equilibrium, the exchange profile of BFRs between water and sediment requires the release of BFRs into water. The risk quotient (RQ) indicated that TBCO in water and ice poses a moderate risk to aquatic organisms, and its potential impact on wetland ecology cannot be ignored.

Metabolite profiles of processed finger millet (Eleusine coracana) and edible crickets (Acheta domesticus) using gas chromatography–mass spectrometry (GC–MS)

Abstract Variations occur in metabolite profiles after food processing, with gas chromatography–mass spectrometry (GC–MS) being one such technique used in profiling metabolites. In this study, finger millet (Eleusine coracana) grains were traditionally (malted and fermented) and also novelly processed (ultra-sonicated), while edible crickets (Acheta domesticus) were fermented and ultra-sonicated. One hundred and nine (109) compounds using gas chromatography–mass spectrometry (GC–MS) in raw and processed finger millet and edible cricket flour were observed and categorized into different metabolite groups: acids, alcohols, amino acids, aromatic compounds, benzene, ethanol, fatty acids, organic acids, and sugars. Significant differences in metabolite profiles, average peak area levels, and the metabolite composition between the finger millet and edible cricket samples before and after traditional and novel processing demonstrate the influence of the processing methods used. Principal component analysis (PCA) observed the relationship among the processing technique’s distribution of metabolite profiles, while OPLS-DA highlighted the significant metabolite profiles observed within the different processing techniques. Differences were observed in the samples as a function of the processing technique used and the modifications, which are attributable to the pre-existing composition of the substrate and the impact of the different techniques, among others. The study’s findings provide a crucial framework for tracking and controlling the metabolite composition of finger millet and edible cricket flours during traditional and novel processing.

Antimicrobial and antiproliferative activity of biosynthesized manganese nanocomposite with amide derivative originated by endophytic Aspergillus terreus

BackgroundScientists have faced difficulties in synthesizing natural substances with potent biological activity from cost-effective sources. Endophytic fungi metabolites with nanoparticles have been utilized to develop a friendly, suitable procedure to address this problem and ameliorate the average amount of antioxidant, antimicrobial, and anticancer materials. Therefore, this study utilized endophytic fungi as a source of the natural extract with biosynthesized manganese nanoparticles (MnNPs) in the form of nanocomposites.MethodsThirty endophytic fungi were isolated and were assessed for their antioxidant activity by 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) and antimicrobial activity. The most potent isolate was identified utilizing 18S rRNA and was applied to purify and separate their natural antimicrobial products by Flash column chromatography. In addition, the most potent product was identified based on instrumental analysis through Nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR), and Gas chromatography-mass spectrometry (GC.MS). The purified product was combined with biosynthsesized manganese nanoparticles (MnNPs) for the production of nanocomposite (MnNCs). Later on, the physicochemical features of MnNPs and its MnNCs were examined and then they were assessed for determination their biological activities.ResultsThe most potent isolate was identified as Aspergillus terreus with accession number OR243300. The antioxidant and antimicrobial product produced by the strain A. terreus was identified as an amide derivative consisting of 3-(2-Hydroxy-4,4-dimethyl-6-oxo-1-cyclohexen-1-yl)-4-oxopentanoic acid (HDOCOX) with the chemical formula C13H18O5. Furthermore, purified HDOCOX, MnNPs and Mn-HDOCOX-NPs nanocomposite (MnNCs) showed significant antimicrobial effectiveness. The minimum inhibitory concentrations (MICs) determined for MnNCs were 10 µg/mL against C. albicans and E.coli. Furthermore, MnNCs were reduced hepatocellular carcinoma viability.ConclusionThe use of HDOCOX, either alone or in combination with MnNPs, is a potential candidate for inhibiting pathogenic microbes and the development of an anticancer drug pipeline.

Biodegradation and biodetoxification of phenanthrene mixture with anthracene and benzo(a)pyrene; an in-depth study on Phellinus noxius BRB 11 potentials, isolated from Berbak-Sembilang Biosphere Reserve, Indonesia

Biodegradation of PAH mixtures is an eco-friendly practice that requires the exploration of efficient microbes. In this study, three fungal species; Phellinus noxius BRB 11, Leiotrametes menziesii BRB 73, and Ceriporia lacerata BRB 81, were screened for phenanthrene (PHE) degradation. Manganese peroxidase (MnP) and laccase activities were analyzed before and after the degradation. Following the screening, further degradation was performed by P. noxius BRB 11 using the following seven treatments: PHE, anthracene (ANT), Benzo (a) pyrene (BaP), PHE–ANT, PHE–BaP, ANT–BaP, and ANT–PHE–BaP. The transformed metabolites and metabolic pathways were determined by gas chromatography-mass spectrometry (GC-MS). Phytotoxicity and cytotoxicity were investigated using Artemia salina and Vigna radiata. The primary screening revealed P. noxius BRB 11 to be a potential strain based on 95% PHE degradation, followed by 24%, and 26% using L. menziesii BRB 73 and C. lacerata BRB 81, respectively. Further research was carried out using only P. noxius BRB 11 to degrade PAHs in single and mixture forms. Co-degradation was observed above 90% except for ANT, while single degradation demonstrated high values for ANT and lower for BaP. Pathway analysis of P. noxius BRB 11 confirmed ten metabolites with lower toxicity in both phytotoxicity and cytotoxicity assays. This study provides a foundation for the practical applications of white rot fungal species in PAH degradation.

In Place Industrial 2,4,6-Trinitrotoluene(TNT) wastewater biodegradation treatment using obligate aerobic bacteria

Abstract This study aims to introduce biological treatments for the explosive substance 2,4,6-trinitrotoluene (TNT). This material has been scientifically proven to be toxic and the toxicity of its derivatives has been evaluated on living organisms, but does not affect bacteria. Fungi and yeasts, therefore these microorganisms can be used to treat the toxic material TNT and its derivatives that dissolve in water. You can use one of the biotic methods in an environmentally friendly way, such as: B. a bioreactor. Here, a screening for TNT degradation by six selected bacteria revealed that Buttiauxella sp. S19-1 has the strongest degradation ability. Furthermore, BuP34O (a gene encoding protocatechuate 3,4-dioxygenase – P34O, a key enzyme in the β-ketoadipate pathway) was upregulated during TNT idegradation. Knockout of BuP34O in S19-1 to generate the S-M1 mutant strain resulted in a significant reduction in TNT degradation efficiency compared to S19-1. Furthermore, the EM1 mutant strain (Escherichia coli DH5α transfected with BuP34O) showed higher degradation efficiency than DH5α. Gas chromatography-mass spectrometry (GC–MS) analysis of TNT degradation by S19-1 revealed 4-amino-2,6-dinitrotolune (ADNT) as an intermediate metabolite of TNT. Furthermore, the recombinant protein P34O (rP34O) expressed an activity of 2.46 µmol/min·mg. Our results represent the first report of the involvement of P34O in the bacterial degradation of TNT and its metabolites, suggesting that P34O may catalyze downstream reactions in the in the TNT degradation pathway. Furthermore, the TNT-degrading ability of S19-1, a gram-negative marine bacterium, offers enormous potential for the restoration of TNT-contaminated water bodies.

Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies

IntroductionThis study investigated the antioxidant, antimicrobial, and insecticidal properties of Chamaemelum nobile (L.) essential oil (CN-EO), harvested in Taounate, Morocco. The molecular composition and chemical profile of CN-EO were also characterized.MethodsThe CN-EO was extracted using a Clevenger apparatus. Its chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). Antioxidant activity was evaluated using the DPPH assay, while antimicrobial properties were assessed via the disk diffusion method to measure inhibition zones against various bacterial and fungal strains. Insecticidal activity was tested through bioassays to determine insect mortality and repellency rates. Phylogenetic analysis of DNA sequences was conducted to confirm the species identity.ResultsGC-MS analysis identified 24 compounds in CN-EO, with β-Oplopenone (18.66%), Spathulenol (14.90%), and Himachalene (12.47%) as major constituents. CN-EO exhibited strong antioxidant activity (IC50 = 135.8 ± 1.03 μg/mL). Antimicrobial assays revealed inhibition zones of up to 20.67 ± 0.58 mm (Staphylococcus aureus) and antifungal inhibition of 40.42% ± 2.82% against Aspergillus flavus. Insecticidal tests showed total insect mortality at 166 µL/L within 48 h and a 60% repellent effect. Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).ConclusionThese results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. It exhibits a wide range of biological activities and shows great promise as a natural antioxidant, antimicrobial agent, antifungal, and insecticide.

Methyl Jasmonate Enhances the Resistance of Populus alba var. pyramidalis Against Anoplophora glabripennis (Coleoptera: Cerambycidae)

Populus alba var. pyramidalis (PaP) is a very important and main planted tree species in northwestern China. However, it has been threatened by Asian longhorned beetle Anoplophora glabripennis (ALB) infestation. A feasible way to protect PaP is by improving its own insect resistance ability. In order to achieve this goal, we first checked whether ALB could induce the defense system of PaP by comparing the ALB-attracted volatiles of PaP before and after ALB infestation through the collection and identification of volatiles by gas chromatography–mass spectrometry (GC-MS). We found that attractant volatiles (Z)-3-hexenol (Z3H) and (Z)-3-hexen-1-yl acetate (Z3HA) decreased by 72.99% and 74.53% after ALB infestation, respectively. Then, the contents of the plant hormones salicylic acid (SA), jasmonic acid (JA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) and the defense substances hydrogen peroxide (H2O2), peroxidase (POD), and total superoxide dismutase (T-SOD) in the phloem of PaP were determined before and after ALB infestation by high-performance liquid chromatography–mass spectrometry (HPLC-MS) and a manufacturer’s kit, respectively. The results showed that the quantities of SA decreased, but JA and MeJA increased by 2.1 times and 3.02 times, respectively, and the increase in H2O2 and POD was also significant. Therefore, we hypothesized that MeJA might be closely related to the induced ALB resistance of PaP. Further exogenous spraying of MeJA on PaP showed that the feeding and oviposition of ALB adults were significantly decreased on PaP, confirming that MeJA could improve PaP’s resistance against ALB. The concentration effect showed that 10−4 mol/L of MeJA treatment induced the strongest results. Our results clearly demonstrated the response of a poplar species to a wood borer infestation and provide an alternative method to protect PaP in the future.

Metabolites and Free Fatty Acids in Japanese Black Beef During Wet Aging

Abstract: Background: Japanese Black beef is known for its high intramuscular fat content, an important factor in its distinctive Wagyu aroma. Wet aging, which involves vacuum-packing meat and storing it at low temperatures, enhances flavor, texture, and tenderness and is essential for maintaining and improving meat quality. In this study, changes in metabolites and lipid profiles were investigated during the wet aging of Japanese Black and Holstein beef. Methods/Results: Gas chromatography–mass spectrometry identified 113 metabolites in Japanese Black beef and 94 in Holstein beef, with significant increases in metabolites like aspartic acid and maleic acid over the aging period. Regarding lipid composition, total free fatty acids significantly increased with wet aging, with Japanese Black beef showing significantly higher concentrations of oleic and linoleic acids than Holstein beef. Additionally, lipid analysis by liquid chromatography–mass spectrometry revealed a reduction in specific phospholipids, particularly lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), with notable decreases in LPC (18:1), LPC (18:2), LPE (18:1), and LPE (18:2). Conclusions: These results suggest that wet aging influences the stability of membrane lipids, facilitating the degradation of phospholipids into free fatty acids, and improving the flavor of Japanese Black beef.