Sterilization process monitoring and control is key to product safety in the healthcare industry. ISO/AAMI 11137-1 addresses the importance of monitoring and documenting radiation process parameters to ensure products have been processed according to specifications. Standards generally state that any doses delivered to product are cumulative regardless of whether the dose is delivered all at once or whether there is a process interruption, for example to correct an issue with the conveyor system. That is certainly the case with regards to radiation effects on materials (product). It was desired to better understand if multiple doses delivered with a process interruption in between are likewise cumulative with respect to microbial inactivation. Although ISO/AAMI 11137-1 describes the requirement to document process interruption, it does not include references to describe the effect of process interruption on the microbial resistance to radiation.The main objective of this study is to investigate the effect of gamma sterilization process interruption at 4 h, 1 day, and 1 week on the radiation resistance of G. stearothermophilus in both a wet and a dry state.