The various stimuli leading up to the large-scale preparation of anti-Rh(D) gamma globulin in Australia early in 1967 and subsequently are mentioned. The quantitation of anti-Rh(D) gamma globulin, namely, “titres”, by laboratory serum-albumin titration methods, “activity”, by microgram-permillilitre anti-D estimations made in London and in Melbourne, and “survival”, by radioactivity estimations in Sydney of clearance times of 51Cr-tagged red cells conducted in Rh-negative volunteers, and the necessary quality control tests at various stages of the preparation of gamma globulin are discussed.