Gallbladder Lesions Research Articles

Immunohistochemical expression of Her2-neu and Ki-67 in premalignant and malignant lesion of gall bladder and its correlation with survival time

Immunohistochemical expression of Her2-neu and Ki-67 in premalignant and malignant lesion of gall bladder and its correlation with survival time

Computed diffusion-weighted magnetic resonance imaging with high b-values in the diagnosis of gallbladder lesions.

The diagnosis of gallbladder lesions remains challenging. The efficacy of computed diffusion-weighted imaging (DWI) with high b-values and apparent diffusion coefficient (ADC) for the diagnosis of gallbladder cancer remains unknown. We aimed to investigate the usefulness of computed DWI with high b-values and the combination of computed DWI and ADC in differentiating malignant and benign gallbladder lesions. Sixty patients (comprising 30 malignant and 30 benign lesions) who underwent magnetic resonance imaging for gallbladder lesions were included in this retrospective study. Qualitative evaluations were performed using conventional DWI with b1000, computed DWI with b1500, b1000 DWI/ADC, and computed b1500 DWI/ADC, and their diagnostic performances were compared. The sensitivity, specificity, and accuracy of computed b1500 DWI/ADC were 90% (27/30), 80% (24/30), and 85% (51/60), respectively. The accuracy of computed b1500 DWI/ADC was higher than that of conventional b1000 DWI (52%, 31/60, p < 0.001), computed b1500 DWI (72%, 43/60, p = 0.008), and b1000 DWI/ADC (78%, 47/60, p = 0.125). The specificity of computed b1500 DWI/ADC was also higher than that of conventional b1000 DWI (7%, 2/30, p < 0.001), computed b1500 DWI (47%, 14/30, p = 0.002), and b1000 DWI/ADC (67%, 20/30, p = 0.125). No significant difference was observed in the sensitivity between the groups. This study shows that computed DWI with high b-values combined with ADC can improve diagnostic performance when differentiating malignant and benign gallbladder lesions. Computed diffusion-weighted magnetic resonance imaging with high b-values in the diagnosis of gallbladder lesions. *Computed DWI with b1500 combined with ADC can improve diagnostic performance when differentiating gallbladder lesions compared with conventional methods (b1000 DWI).

Metastasis of Spindle Cell Malignant Melanoma in Gallbladder

Malignant melanoma is an aggressive neoplasm with a high tendency to metastasize.Gastrointestinal metastases, although described in the literature, are infrequent. We present the case of a 51-year-old male patient with a surficial spreading melanoma stage IIIc with BRAF mutation who presented a gallbladder outgrowth lesion, compatible with a polyp. A significant growth of the lesion was observed in subsequent TC studies and a laparoscopic cholecystectomy was performed. The anatomopathological study of the specimen confirmed the diagnosis of gallbladder metastasis due to epithelioid and spindle cell malignant melanoma. The presence of a gallbladder lesion in the context of a patient diagnosed with melanoma should establish the diagnostic suspicion of metastasis, and an early extension study and laparoscopic cholecystectomy should be considered. The palliative surgical approach to avoid hepatobiliary symptomatology can be considered.

Immunohistochemical Expression of Her2neu in Premalignant and Malignant Lesion of Gall Bladder

Purpose: To evaluate the expression of Her2-neu in benign and malignant gallbladder lesions, and to establish correlations with clinico-pathologic parameters. Materials and Methods: Retrospective and prospective analysis was conducted on formalin fixed paraffin embedded (FFPE) 154 tissue sample included Premaligant and premalignant like lesions (including dysplasia, metaplasia and xanthogranulomatous cholecystitis)(n=47) and malignant gallbladder (n=85) with 22 cases of chronic cholecystitis as control. Hematoxylin and eosin stained slides of each case were reviewed for: type of malignancy (whether adenocarcinoma, squamous cell carcinoma or any other type), grade (well, moderate, and poor). Immunohistochemistry for Her 2 neu was performed and data analysis was conducted using SPSS 15.0 software. P value of ≤0.05 was considered significant. Results: The difference of Her 2 neu expression between benign and malignant groups was found to be statistically significant. Her2/neu positivity did not have any significant correlation with various clinicopathological parameters. Conclusions: The present study demonstrated overexpression of Her2-neu in gallbladder cancer. A trend of decreasing Her2/neu expression with increasing grade of tumor was observed. The results however are encouraging and suggest evaluation of Her2/neu as a candidate for targeted therapy.

Transferring a naso-gallbladder drainage tube to the mouth for re-examination of a gallbladder lesion.

Conventional gallbladder re-intervention along the naso-gallbladder drainage tube may result in dislocation of the tube during scope insertion or failure of guidewire insertion into the gallbladder. Mandai et al report a simple method for transferring a transpapillary naso-gallbladder drainage tube to the mouth that facilitates reliable re-intervention for gallbladder lesions.

Utility of Image-Guided Fine-Needle Aspiration Cytology from Neoplastic Hepatic and Gallbladder Lesions

Introduction: Fine-needle aspiration cytology (FNAC) is an important and successful method for liver and gallbladder (GB) lesion diagnosis. Aspiration from liver and GB masses and its cytological evaluation differentiate whether it is benign or malignant. It is of huge help to the clinician for deciding on timely and appropriate management of the patient. Image-guided FNAC of liver lesions helps to know lesion localization whether it is multifocal, with the benefit of multiple passes for adequate sampling. Aims and Objective: This study aims at finding the importance and effectivity of image-guided FNAC of liver and GB masses with the main focus on the diagnosis of neoplastic lesions. Materials and Methods: The study was a prospective study conducted in the Cytology Section in the Department of Pathology, Gajra Raja Medical College, Gwalior, from January 2019 to March 2021. All the slides of liver and GB fossa mass aspiration done with help of image guidance were stained and examined. Results: There were a total of 86 cases, out of which six cases had unsatisfactory material, thus rendering them as nondiagnostic (7%). The diagnostic efficacy is 93%. Out of 80 cases, 71 (87.5%) were reported neoplastic and 9 (12.5%) were nonneoplastic. Among neoplastic lesions (71/80, i.e., 88.7%), 70 were malignant and one case was reported as suspicious for malignancy. Out of 71 neoplastic lesions, 39 were from liver mass and 32 were from GB fossa. Among 38 malignant neoplastic tumors of the liver, six cases were hepatocellular carcinoma (15.7%). Out of 32 metastatic lesions to the liver, 30 were of adenocarcinoma and one case each of small-cell morphology and lymphoma. Conclusions: Cytological evaluation is an important tool and can be applied as the first line of diagnostics in the management of liver masses and can characterize liver masses. GB lesion cytology has more diagnostic yields and accuracy of differentiating neoplastic lesions from nonneoplastic lesions. Hence, from the study, we can conclude that advanced techniques in the cytological study of liver and GB mass can give an early diagnosis.

Isolated IgG4-related cholecystitis with localized gallbladder wall thickening mimicking gallbladder cancer: a case report and literature review

BackgroundIgG4-related cholecystitis, which is a manifestation of IgG4-related disease in the gallbladder, is associated with autoimmune pancreatitis or IgG4-related sclerosing cholangitis in most cases; isolated gallbladder lesions without systemic manifestations are very rare. Gallbladder wall thickening is often diffuse, but sometimes localized, in which case, differentiation from gallbladder cancer becomes difficult. The characteristic features of IgG4-related cholecystitis on imaging that would enable differentiation from gallbladder cancer remain poorly described.Case presentationWe present a rare case of isolated IgG4-related cholecystitis with localized gallbladder wall thickening that was clinically difficult to distinguish from malignancy before resection. An 82-year-old man was referred to our hospital because of gallbladder wall thickening on abdominal ultrasonography without any symptoms. Dynamic computed tomography of the abdomen showed localized wall thickening from the body to the fundus of the gallbladder that was enhanced from an early stage with a prolonged contrast effect. There were no other findings, such as pancreatic enlargement and bile duct dilatation. Magnetic resonance cholangiopancreatography revealed neither dilatation nor stenosis of the bile duct and pancreatic duct. Endoscopic ultrasonography (EUS) showed a smooth layered thickening of the gallbladder wall with a maximum thickness of 6 mm and a well-preserved outermost hyperechoic layer in the same area. Laparoscopic cholecystectomy was performed because malignancy could not be completely ruled out. Pathological examination of a resected specimen revealed IgG4-positive plasma cell infiltration, fibrosis, and phlebitis. Although the serum IgG4 level measured after resection was normal, the condition was ultimately diagnosed as probable IgG4-related cholecystitis according to the 2020 revised comprehensive diagnostic criteria for IgG4-related disease. The EUS images reflected the pathological findings, in which lymphocytic infiltration was distributed in a laminar fashion in the gallbladder wall.ConclusionsAlthough rare, isolated IgG4-related cholecystitis with localized wall thickening mimicking gallbladder cancer remains a clinical problem. A smooth laminar thickening of the gallbladder wall on EUS imaging could be one of the most informative characteristics for differentiating IgG4-related cholecystitis from gallbladder cancer.

Precursor Lesions of Gallbladder Carcinoma: Disease Concept, Pathology, and Genetics.

Understanding the pathogenesis and carcinogenesis of gallbladder adenocarcinoma is important. The fifth edition of the World Health Organization’s tumor classification of the digestive system indicates three types of preinvasive neoplasm of the gallbladder: pyloric gland adenoma (PGA), biliary intraepithelial neoplasia (BilIN), and intracholecystic papillary neoplasm (ICPN). New terminologies have also been introduced, such as intracholecystic papillary-tubular neoplasm, gastric pyloric, simple mucinous type, and intracholecystic tubular non-mucinous neoplasm (ICTN). Pancreatobiliary maljunction (PBM) poses a markedly high risk for bile duct carcinoma, which was analyzed and investigated mainly by Asian researchers in the past; however, recent studies have clarified a similar significance of biliary carcinogenesis in Western countries as well. In this study, we reviewed and summarized information on three gallbladder neoplastic precursors, PGA, BilIN, and ICPN, and gallbladder lesions in patients with PBM.

Mucinous Cystadenoma of Gall Bladder: A Rare Entity

Cystic lesions of gall bladder are very rare. Most of the cystic cholecystic pathologies are inflammatory or perihepatic cystic lesions. Only few primary cystic lesions are known and being rare pose a diagnostic and therapeutic challenge. Out of these mucinous cystadenoma(MCN) is the rarest condition and we report one such case and discuss its differential diagnosis.

Differential Diagnosis of Benign and Malignant Gallbladder Lesions Using CT Based Machine Learning

Introduction: Gallbladder cancer is a tumor of extremely high malignancy. However, in most cases, patients with gallbladder cancer are detected at a late stage and miss the opportunity for radical resection. Therefore, early and correct diagnosis of gallbladder cancer is the key to provide the best treatment for patients.

Histopathologic Profile of Premalignant and Malignant Lesions of Gallbladder

Introduction: Neoplastic lesions of gallbladder are less well defined than other lesions in gastrointestinal tract due to rarity of lesions and also poor accessibility of this site. More than fifty percent cases of cancer are clinically missed. This study was aimed to see the histopathologic spectrum of premalignant and malignant lesions, compare clinical diagnosis with histopathological diagnosis and see association between gross morphology and missed clinical diagnosis. Methods: This study was conducted on 56 consecutive cases of premalignant and malignant lesions of gallbladder received in study period of 10 years from January 2011 to December 2020. Results: Age of the patients ranged from 13 – 84 years with a mean±SD of 56.8±13.7 years with maximum 17 (30.4%) cases in 41 – 50 years age group. There were 14 (25.0%) males and 42 (75.0%) females with a male female ratio of 1:3. 10 (17.9%) cases were premalignant lesions and 46 (82.1%) cases were malignant lesions. 93.5% malignant cases were adenocarcinoma. 41.3% cases were clinically not suspected to be malignant. There was no association between gross morphology and missed clinical diagnosis (p &gt; 0.05). Conclusions: Routine histopathologic examination of all the resected gallbladder specimens is manadatory as important diagnosis can be missed.

Determinative Structural Features Identified With Probe-based Confocal Endomicroscopy for the Accurate Diagnosis of Gallbladder Malignancy.

Probe-based confocal laser endomicroscopy (pCLE) can visualize microscopic structures at high resolution but has not yet yielded definitive diagnostic features of gallbladder malignancy, as opposed to benign changes. A total of 73 patients had their gallbladder evaluated with pCLE performed on resected benign and malignant gallbladder surgical specimens, which were sprayed with fluorescein. Malignant and benign features of pCLE findings were identified on the basis of Miami and Paris Classifications. Standard histopathological diagnoses and individual patient pCLE findings of gallbladder lesions were correlated. Of the 73 consecutive patients that had their gallbladder evaluated ex vivo with pCLE, 11 were identified with gallbladder malignancy. pCLE identified features of gallbladders examined ex vivo, including the presence of thick dark bands and dark clumps, which together correlated with histopathologically-determined biliary malignancy at 100% sensitivity. Thick white bands and visualized epithelium, also identified with pCLE, together correlated with histopathologically-determined malignancy at 100% specificity. pCLE can be used for real-time differentiation of cancerous/non-cancerous regions in the gallbladder using the diagnostic criteria identified in the present study.

P-L04 Temporal changes in prevalence and associated risk factors for gallbladder dysplasia and adenocarcinoma in patients undergoing cholecystectomy. A UK hospital-based observational study

Abstract Background A metaplasia-dysplasia–carcinoma sequence is the most plausible carcinogenic pathway for gallbladder cancer. Although the incidence of gallbladder carcinoma is increasing, little is known about its precancerous lesions. The aim of this study was to determine temporal changes in the prevalence of low-grade dysplasia (LGD), high-grade dysplasia (HGD) and gallbladder adenocarcinoma and associated risk factors. Methods We retrospectively identified consecutive patients who underwent cholecystectomy between January 2011 and March 2020. Patients were grouped according to histology: no dysplasia; LGD; HGD; and adenocarcinoma. Fitted linear models estimated temporal trends in prevalence and mean age for all histological outcomes. Logistic regression estimated associated risk factors. Results A total of 5 835 patients were included in the analysis. The prevalence of LGD was 1.47%, HGD 0.17% and adenocarcinoma 0.19%. Prevalence for all diseases increased over time, and mean age at diagnoses decreased over time. In a multivariate logistic regression model, with no dysplasia as the reference group, female sex increased the odds of LGD (OR 4.57, 95% CI 3.07-10.10, p = &amp;lt;0.0001). BMI was not associated with disease risk. Conclusions Our data suggests the prevalence of precancerous gallbladder lesions are increasing in younger patients. Although a risk factor for cholelithiasis, BMI was not associated with disease progression. If occurring in a dysplasia-carcinoma sequence, mean age of diagnoses suggests a progression period of 20 years. Further research is required to explain both the significant sex disparity and potential environmental risk factors for gallbladder dysplasia.

Contrast enhanced ultrasound versus multiphasic contrast enhanced computed tomography in evaluation of gallbladder lesions.

To compare the diagnostic performance of contrast enhanced ultrasound (CEUS) and multiphasic contrast enhanced computed tomography (CECT) in differentiating benign and malignant gallbladder (GB) lesions. This prospective ethical review board approved study comprised consecutive patients with GB lesions who underwent CEUS and multiphasic CECT at a tertiary care referral center. The enhancement patterns at CEUS and CT were compared. The quantitative CEUS parameters including arrival time (AT), AT in liver, time to peak enhancement, and washout time (WT) were assessed. The diagnostic performance of CEUS and CT features was calculated using receiver operating characteristic analysis. A subgroup analysis was performed for patients with GB wall thickening. Multivariate analysis was performed to identify features significantly associated with malignancy. Over the study period, 30 patients (mean age, 52.8 ± 12.2years, 17 females) with GB lesions were evaluated. Benign and malignant diseases were present in 13 and 17 patients, respectively. There was excellent agreement between CEUS and CT findings. Among the quantitative CEUS features, only WT was significantly associated with malignancy in the overall group (p < 0.001) and wall thickening subgroup (p = 0.007). WT within 53.5s and 51.5s had sensitivity of 88.2% and 81.8% and specificity of 84.5% and 100% in diagnosing malignant lesions in the overall group (AUC 0.900) and the wall thickening subgroup (area under curve, AUC 0.927), respectively. At multivariate analysis, features that were significantly associated with malignant lesions in the overall group were disruption of GB wall (CEUS), intralesional non-enhancing areas (CEUS), liver involvement (CEUS or CT), and arterial phase hyperenhancement (CT) in the overall group and disruption of GB wall (CEUS), WT (CEUS), and liver involvement (CEUS or CT) in the wall thickening subgroup. CEUS is a useful adjunct to CT in evaluation of GB lesions. Its utilization in patients with GB wall thickening may improvedetection of malignancy.

A Novel Endoscopic Ultrasonography Imaging Technique for Depicting Microcirculation in Pancreatobiliary Lesions without the Need for Contrast-Enhancement: A Prospective Exploratory Study.

Detective flow imaging endoscopic ultrasonography (DFI-EUS) provides a new method to image and detect fine vessels and low-velocity blood flow without using ultrasound contrast agents. The aim of this study was to evaluate the utility of DFI-EUS for pancreatobiliary lesions and lymph nodes. Between January 2019 and January 2020, 53 patients who underwent DFI-EUS, e-FLOW EUS, and contrast-enhanced EUS were enrolled. The ability of DFI-EUS and e-FLOW EUS to detect vessels was compared with that of contrast-enhanced EUS. This article describes the DFI technique along with our first experience of its use for vascular assessment of pancreatobiliary lesions. Vessels were imaged in 34 pancreatic solid lesions, eight intraductal papillary mucinous neoplasms (IPMNs), seven gall bladder lesions, and four swollen lymph nodes. DFI-EUS (91%) was significantly superior to e-FLOW EUS (53%) with respect to detection of vessels (p < 0.001) and for discrimination of mural nodules from mucous clots in IPMN and gallbladder lesions from sludge (p = 0.046). Thus, DFI-EUS has the potential to become an essential tool for diagnosis and vascular assessment of various diseases.

Non-neoplastic Lesions of Gallbladder Among Cholecystectomy Specimens of a Tertiary Care Center: A Descriptive Cross-sectional Study.

Gallbladder diseases are prevalent worldwide and present with a diverse histopathological spectrum. Mucosal irritation and chronic inflammation is considered as an important etiological factor for the mechanical or functional dysfunction of emptying of the gallbladder. This study aims to find the prevalence of non-neoplastic lesions of gallbladder among cholecystectomy specimens of a tertiary care center. A descriptive cross-sectional study was conducted in the Department of Pathology, of a tertiary care center from January 2005 to December 2020. Ethical approval was taken from the Institutional Review Committee. All the patients who had undergone cholecystectomy procedures which showed non-neoplastic lesions were enrolled in the study. Convenient sampling was done. Statistical Package for Social Sciences version 21 and Microsoft Excel were used for data analysis. Point estimate at 95% Confidence Interval was calculated along with frequency and proportion for binary data. Out of 4914 cholecystectomy specimens, 4852 (98.73%) (95% Confidence Interval= 98.42- 99.04) were non-neoplastic lesions. There were 1252 (25.8%) males and 3600 (74.2%) females with a male to female ratio of 1:2.87. Age ranged from 2 to 89 years with a mean age of 45±14.48 years. Gallbladder lesions were observed maximum in age group 41-50 years with 1200 (24.7%) cases. Among the non-neoplastic lesions, cholecystitis without any specific finding was the most common finding with 3028 (62.4%) cases followed by cholelithiasis with 1478 (30.5%) cases. The prevalence of non-neoplastic lesions of gallbladder is similar to other studies donein similar setings. Female predominance was noted in non-neoplastic lesions.

Ultrasonographic evaluation of hepatobiliary diseases

Background and Objectives: The science of Diagnostic ultrasound is perhaps the most promising of the imaging modalities currently available. The main objective of this study is to establish the efficacy of ultrasonography to detect various hepatobiliary lesions and provide necessary information, aiding in their appropriate management. Methods: This study, carried over a period of two years, included 150 patients with various hepatobiliary lesions. There were patients of both sexes and their ages were above 5 years and below 85 years. After the routine blood and urine investigations and liver function tests, the patients were subjected to ultrasound examination. Results: One fifty cases, aged between 5 and 85 years, with various hepatobiliary lesions were studied with real time ultrasonography. The most frequently encountered Gall bladder lesion was Gallstones (44) with associated chronic cholecystits in 32 cases, acute cholecystitis in 10 cases, Gall bladder carcinoma in 2 cases. There were 3 cases of GB polyp, 2 case of chronic cholecystitis, 1 case of CA Gallbladder which were not associated with gall stones. Among the CBD lesions there were 2 cases of choledocholithiasis, 2 cases of cholangiocarcinoma and 1 case of choledochal cyst. The most frequently encountered focal liver lesion in this study was amoebic liver abscess which was found in 20 cases. The second most commonly encountered focal liver lesion was hepatocellular carcinoma which was found in 12 cases. 10 cases with hepatic metastasis were found. 4 cases with ultrasound features highly suggestive of haemangioma and 2 cases with hepatic hydatid disease were found. One case each of an intrahepatic haematoma and a simple liver cyst was found. Among the diffuse liver diseases, the most commonly encountered was fatty liver which was seen in 16 cases. The second most common diffuse liver disease was cirrhosis which was seen in 12 cases. 10 cases of acute hepatitis, 4 cases of chronic hepatitis and 3 cases of congestive liver was found. Conclusion: Ultrasound proved to be a sensitive, easily available and safe imaging modality for the diagnosis and follow-up of various hepatobiliary lesions and should therefore be the first line of investigation in suspected cases.

The Role of Endoscopic Ultrasound in the Diagnosis of Gallbladder Lesions.

Gallbladder (GB) diseases represent various lesions including gallstones, cholesterol polyps, adenomyomatosis, and GB carcinoma. This review aims to summarize the role of endoscopic ultrasound (EUS) in the diagnosis of GB lesions. EUS provides high-resolution images that can improve the diagnosis of GB polypoid lesions, GB wall thickness, and GB carcinoma staging. Contrast-enhancing agents may be useful for the differential diagnosis of GB lesions, but the evidence of their effectiveness is still limited. Thus, further studies are required in this area to establish its usefulness. EUS combined with fine-needle aspiration has played an increasing role in providing a histological diagnosis of GB tumors in addition to GB wall thickness.

The Value of Combined Application of Oxycodone Hydrochloride Injection and Dexmedetomidine in Anesthesia for LC for Patients with Gallbladder Lesions.

Objective To analyze the effect of combined application of oxycodone hydrochloride injection and dexmedetomidine in anesthesia for laparoscopic cholecystectomy (LC) for patients with gallbladder lesions. Method 93 patients with gallbladder lesions in our hospital were divided into 2 groups by the random number table method. 46 patients in the control group applied oxycodone hydrochloride injection in anesthesia, and 47 patients in the observation group applied oxycodone hydrochloride injection combined with dexmedetomidine in anesthesia. Result The T1 and T2 MAP levels in the observation group were lower than those in the control group (P < 0.05), and the difference between T3 and the control group was not significantly significant (P > 0.05). The T1 to T3 HR level in the observation group were lower than those in the control group (P < 0.05). The rate of excessive sedation (10.64%) and sedation inefficiency (12.77%) in the observation group was lower than that in the control group (28.26% and 30.43%), and the rate of satisfactory sedation (76.60%) was higher than that in the control group (41.30%) (P < 0.05). The postoperative awakening, tracheal tube removal, and first anal venting time were shorter in the observation group than in the control group (P < 0.05). The WHO scores of incisional pain at 6, 12, 24, and 48 hours after the operation were lower in the observation group than in the control group (P < 0.05). The T2 SOD level in the observation group was higher than that in the control group, and the ROS and MDA levels were lower than those in the control group (P < 0.05). The incidence of side effects of anesthetic in the observation group was 17.02%, which was not statistically different from the control group of 13.04% (P > 0.05). Conclusion The combined application of oxycodone hydrochloride injection and dexmedetomidine in anesthesia for LC for patients with gallbladder lesions can achieve better sedation and analgesia effect, accelerate postoperative awakening and recovery, and control oxidative stress and fluctuations in signs, without increasing anesthesia-related side effects.

The Role of EUS and EUS-FNA in Differentiating Benign and Malignant Gallbladder Lesions.

Endoscopic ultrasonography (EUS) has greater spatial resolution than other diagnostic imaging modalities. In addition, if gallbladder lesions are found and gallbladder cancer is suspected, EUS is an indispensable modality, enabling detailed tests for invasion depth evaluation using the Doppler mode and ultrasound agents. Furthermore, for gallbladder lesions, EUS fine-needle aspiration (EUS-FNA) can be used to differentiate benign and malignant forms of conditions, such as xanthogranulomatous cholecystitis, and collect evidence before chemotherapy. EUS-FNA is also useful for highly precise and specific diagnoses. However, the prevention of bile leakage, an accidental symptom, is highly important. Advancements in next-generation sequencing (NGS) technologies facilitate the application of multiple parallel sequencing to EUS-FNA samples. Several biomarkers are expected to stratify treatment for gallbladder cancer; however, NGS can unveil potential predictive genomic biomarkers for the treatment response. It is believed that NGS may be feasible with samples obtained using EUS-FNA, further increasing the demand for EUS-FNA.