Galanga Rhizome Research Articles

FORMULASI SABUN PEMBERSIH KEWANITAAN (Feminime Hygiene) DARI MINYAK ATSIRI RIMPANG LENGKUAS PUTIH (Alpinia galanga L) DAN UJI AKTIFITAS ANTISEPTIK TERHADAP Candida albicans

ABSTRACTCandida albicans is a normal flora in the female genitalia which can cause vaginal discharge. White galangal rhizome contains essential oils of eugenol which have activities that inhibit the growth of Candida albicans. This study formulated the white galangal rhizome essential oil in the form of feminime hygiene to make it easier and more comfortable to use and to determine the antiseptic feminime hygiene activity of the white galangal rhizome oil of Candida albicans. The essential oil of white galangal rhizome is formulated in three variations of concentration namely 10%, 15% and 20%. Feminime hygiene of essential white galangal rhizome oil was evaluated such as organoleptic, homogeneity, specific gravity, foam power test, viscosity, pH and stability. Antiseptic activity testing was tested with SDA (Sabouraud Dextrose Agar) media and using agar diffusion method. The results of the feminime hygiene evaluation formulated showed results similar to the results of comparative evaluations (BN). The parameters of antiseptic activity are indicated by the formation of inhibitory zone diameters where F0 (negative control), F1 (10%), F2 (15%), and F3 (20%) respectively have an average diameter value of 11 mm, 14.65 mm 17.58 mm and 25.41 mm. The classification category of the response to microbial growth according to CLSI, F0 and F1 includes the weak group, the F2 is the moderate group and the F3 is the strong group. Based on the results of one-way ANOVA statistical analysis there were significant differences in liquid soap preparations F0, F1, F2 with F3 at (P <0.05). White galangal rhizome essential oil can be formulated into feminine hygiene and has antiseptic activity against the fungus Candida albicans.Keywords: white galangal, antiseptic, candida albicans

Urolithiasis: Population analysis and composition

Kaempferia galangal L. (Zingiberaceae), also known as aromatic ginger, is used in the food and cosmetics, pharmaceuticals and maquillage materials, as well as in the traditional Indian medicine. The essential oil (EO) obtained from its rhizome by hydrodistillation is of economic interest and contains ethyl p-methoxycinnamate as the main component, which is used as an anticancer, antimicrobial, skin protector and whiteneing and nematicidal agent. To our knowledge, the mosquitocidal properties of the aromatic ginger EO have hitherto been poorly investigated. Therefore, in the present work, we tested this EO and its major chemical constituents (MCCs), namely ethyl p-methoxycinnamate, trans-ethyl cinnamate and trans-cinnamaldehyde for their larval toxicity on Aedes vittatus and Anopheles maculatus. The EO chemical composition, studied by GC-MS analysis, was made up of 39 compounds, with ethyl p-methoxycinnamate (30.6%), trans-ethyl cinnamate (26.8%) and trans-cinnamaldehyde (11.5%) as the major constituents. The EO and its MCCs showed LC50 values of 39.22, 10.36, 28.26 and 36.35 μg/ml on Ae. vittatus, and of 41.36, 12.56, 30.23 and 38.47 μg/ml on An. maculatus third instar larvae, respectively. The ecofriendliness of EO and MCCs on the aquatic fauna was demonstrated as they were safe to Anisops bouvieri, Acilius sulcatus and Gambusia affinis. Our findings showed that the K. galanga rhizome EO and its MCCs are promising larvicidal agents without affecting non-target organisms (NTOs). They also give news insights into the potential economic exploitation of this EO and its MCCs in the fabrication of effective and eco-friendly mosquitocidal formulations.

Antifungal Cream Preparation of Galangal Rhizome Extract (Alpinia galanga L.)

Cream is a semi-solid emulsion dosage form of both water-in-oil (W/O) or oil in water (O/W) type containing one or more dissolved or dispersed ingredients in the corresponding base material (containing no less than 60% water). Cream is usually used as emollient or containing active pharmaceutical ingredients on the skin (Ansel, 2008). The advantage of cream are the application practicallity, water washeability, and the easiness to spread evenly. In this research, we formulated cream containing rhizome Galangal rhizome. According to Darmono (2008), Galangal rhizome has various properties such as antifungal and antibacterial activities. Galangal rhizome contains 1-asetoksikhavikol asetat (ACA). ACA is an antifungal. ACA has good solubility in 70% ethanol. We maserated Galangal rhizome (Alpinia galangal L.) to extract ACA from the simplicia. As for the cream base, we use hidrophilic base containing emulgators stearic acid and triethanolamine, with glycerin as humectant. During the optimization, we chose three formulas, formula 1 (10% stearic acid , 2% triethanolamin, 5% glycerin, and 0.01% vitamin E), formula 2 (15% stearic acid , 3% triethanolamin, 10% glycerin, and 0.05% vitamin E), and formula 3 (20% stearic acid, 4% triethanolamin, 7.5% glycerin, and 0.09% vitamin E). We used the bases to contain 10% of the extract. The results show that formula 1, formula 2, and formula 3 had typical smell of Galangal rhizome, brown color, and thick consistency. All formulas are homogenous. Formula 1 the best stability. We conclude that Galangal rhizome (Alpinia galanga L.) can be formulated in cream form with our formula 1 had the best stability among others.

Alkaloids piperine in dichloromethane fraction of red galangal rizhome (Alpinia purpurata)

Red galangal rizhome (Alpiniapurpurata) was extracted with dichloromethane using maceration methods. The crude extract was fractionated using vacuum column chromatography with a mixture of ethyl acetate:n-hexane (2:3) to give two fractions (F1 and F2). Fraction 2 was fed into the silica gel of column chromatography tailed by recrystallization to produce crystalline (17 mg). The purity was tested by using melting point apparatus and thin-layer chromatography method for any solvent. Their structures were established by spectroscopic methods (UV-Vis, FTIR, 1H-NMR and 13C-NMR). Result of analysis gave melting point of crystals around 130°C. The UV-Vis spectrometer obtained maximum wavelength 344 nm. FTIR analysis showed C=O carbonyl, C-O ether, C-N, and C=C aromatic. Analysis by 1H-NMR spectrometer showed that bioactive compounds have a 19 protons. Analysis by 13C-NMR spectrometer showed that bioactive compounds have a 17 carbon atoms. The results of identification showed that bioactive compounds in dichloromethane fraction of red galangal rhizome was a compound piperine alkaloids types with a chemical formula of C17H19O3N.

Growth profile of Aspergillus niger on red galangal rhizomes as shown by bioactive compound changes

This study we evaluated the growth profile of A. niger in red galangal rhizomes. The fermentation of red galangal rhizome was monitored based on hyphae characteristics followed by extraction with ethanol and determination of the total phenolic using Folin-Ciocalteu method and flavonoids using AlCl3 method. The metabolite profile was studied using Liquid chromatography-mass spectrometry. The results showed that A. niger was able to grow optimally on the 14th day of fermentation. The total phenolic and total flavonoids increased significantly compared to un-fermented product. LCMS profiles also showed the changes in the metabolite arrangement of fermented product comparing to the unfermented extract. There is 4-o-methyl-gallic acid produced by fermentation, which contributed to the increase in total phenolic content.

The potential use of plant extracts for controlling northern leaf blight on organic sweet corn production

Northern leaf blight caused by Helminthosporiumturcicum is the most frequently occurring foliar diseases in sweet corn plants grown under humid environments. Finding an effective control measure for controlling the disease can be a challenging issue for organic growers. The present study was undertaken to investigate the potential use of plant products as the botanical fungicides for controlling northern leaf blight in sweet corn. The aqueous solutions of extracts mixture and its individual constituent extracts made from neem leaf, betel leaf, clove leaf, lemon grass leaf, and galangal rhizome were tested in petri dishes assay for their antifungal activity against H. turcicum. Lemongrass+galangal rhizome exhibited the most effective solution in suppressing the growth of H. turcicum (55%), as followed by galangal rhizome (50%), lemongrass+clove leaf (50%), betel leaf+galangal rhizome (50%), and cloveleaf+neem leaf(47.5%). Accordingly, these 5 solutions were selected and tested for their efficacy in controlling northern leaf blight on sweet corn plants under field condition. Lemongrass+galangal rhizome, galangal rhizome, lemongrass+clove leaf, betel leaf+galangal rhizome, and cloveleaf+neem leaf had similar effectiveness in reducing disease intensity ranged from 55% to 66% and leaf disease severity ranged from 44% to 52% as compared to the control. Betel leaf+galangal rhizome was relatively effective in reducing disease intensity (43.4%) but less effective in reducing leaf disease severity (24%). Application of the selected solution had reduced the ear length but improved the ear weight excluding betel leaf + galangal rhizome.

Enhancing resilient property of Kaempferia galanga rhizome starch by succinylation

The present study investigates on the succinylation of native starch isolated from the rhizomes of Kaempferia galanga. Succinylated starch with different degree of substitution in the range of 0.030–0.189 was prepared using varying concentrations of succinic anhydride. Morphological study showed the formation of porous structure on the outer surface of the starch granules, whereas FTIR spectra showed the presence of ester group on the modified starches. With the increase in degree of substitution, there was an increased amylose content and less fraction of amylopectin and thereby modified starch samples showed relatively less water retention capacity and faster disintegration. The swelling and solubility power of native and modified starches were found to increase with increase in temperature. From the TGA analysis, the succinylated starches were found to be more thermally stable than native starch. In vitro drug release study also supports the disintegration pattern and proves the potential of succinylated Kaempferia galanga starch as resilient material in sustained-drug release systems.

Green Synthesis Nanopartikel Perak (Agnps) Terkonjugasi Etil Parametoksi Sinamat (Epms) sebagai Bahan Tabir Surya

Green Synthesis is a method to make silver nanoparticles (AgNPs) by utilizing natural materials as bioreductors. One of the natural materials that can reduce metal ions is Kaempferia galanga rhizome because it has a chemical component that can penetrate the metal. The compounds are ethyl paramethoxycinnamate (EPMC) which is used as sunscreen. This study aims to produce AgNPs conjugated with EPMC that can be used as raw material sunscreen. This study was conducted by extracting EPMS from the Kaempferia galanga rhizome and synthesizing the AgNPs conjugated with EPMC. The synthesis results were characterized by UV-Vis Spectrophotometry, FTIR, SEM, PSA and sunscreen activity. Extracted result was obtained crystalline is ethyl paramethoxycinnamate compound with yield 2,3%. The characterization of EPMC with UV-Vis spectrophotometer was obtained maximum wavelength of 308 nm and FTIR analysis result at wave number 1701,27 cm-1 indicated the presence of carbonyl group (C = O) and 1165,97 cm-1 presence of C-O and showed specific characteristic from EPMS. Characterization of AgNPs using Uv-Vis spectrophotometer was obtained maximum wavelength of 469 nm. Characterization of AgNPs using SEM are spherical and monodispers forms. Then, particle size using PSA obtained an average diameter of 182 nm. AgNPs conjugated with EPMS has a sunscreen activity with sunblock category and successfully provide protection against UV exposure at low concentration (200 ppm) categorized as ultra protection with SPF value 36,4.

Identification of Endophytic Fungi from Rhizome Galanga (Alpinia galanga L.) against Candida albicans

The main objectives of this research were to isolat and identify endophytic fungi antagonists of Candida albicnas from galanga rhizomes. Samples of galanga rhizome were collected from Gitgit plantation in Gitgit village, Buleleng regency. Isolation and identification were conducted in Food Microbiology Laboratory, Faculty of Agriculture, Udayana University. There were three isolates identified with inhibitory persentage againts Candida albicans i.e. Trichoderma viridae (26.07±3.76%), Trichoderma harzianum (45.11±5.84%) and Fusarium oxyforum (20.11±5.61%).

Kaemgalangol A: Unusual seco-isopimarane diterpenoid from aromatic ginger Kaempferia galanga

A new unusual seco-isopimarane, kaemgalangol A (1) and 12 usual analogs (2−13) were isolated from the rhizomes of Kaempferia galanga (Family: Zingiberaceae). KaemgalangolA (1) represented a rarely isolated 9,10-seco-isopimarane skeleton. The chemical structures of the isolated compounds were mainlyinvestigated by spectroscopic techniques such as 1D, 2D NMR, and HRMS. The absolute configuration of 1–3 was studied by X-ray diffraction analysis as well as experimental and TDDFT-calculated electronic circular dichroism. Among the isolated diterpenoids, 5, 6 and 9 exhibited cytotoxic activity against HeLa (IC50 75.1, 74.2 and 76.5 μM, respectively) and HSC-2 (IC50 69.9, 53.3 and 58.2 μM, respectively) cancer cells.

Direct Amidation of ethyl p-methoxycinnamate to Produce N,N-bis-(2-hydroxyethyl)-p-methoxycinnamamide

Ethyl p-methoxycinnamate (EPMC) (1) is found as a major natural ester in the rhizome of Kaempferia galanga (kencur) and has been known to have various pharmacological activities. The previous study has reported the synthesis of N,N-bis-(2-hydroxyethyl)-p-methoxycinnamamide (2) by using microwave-assisted direct amidation of EPMC (1) with diethanolamine. In this research, we attempt to synthesize of 2 by using a conventional direct amidation of EPMC (1) with diethanolamine. The reaction was conducted without adding any coupling reagents or catalyst. Structure of the synthetic product was determined by using analysis of GC-MS, IR, and 1H-NMR spectroscopic data and then compared to the previously reported.DOI:http://dx.doi.org/10.15408/jkv.v4i1.7300

Pengendalian Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus) dengan Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata)

MAS diseases (Motile Aeromonas Septicaemia) is caused by the bacterium Aeromonas hydrophila. One of the natural ingredients that can be used as an alternative to inhibit bacterial activity is the extract of red galangal rhizome (Alpinia purpurata). Active substances possessed of this material have potential as antibacterial. The purpose of this study is to determine the effectiveness of red galangal rhizome extract in the control of MAS disease in tilapia. The type of this research is experimental research in Completely Random Design (RAL) with 6 treatments, which are positive control, negative control, 250, 500, 750, and 1000 ppm. The result of the research on the activity of the galangal extract bacteria on A. hydrophila obtained the widest clear zone at 1000 ppm constipation, which is 9.7625 mm. The treatment has been conducted by immersion of Tilapia fish into the extracted red galangan rgizomes for 5 minutes, then it was put into aquarium for maintenance for 7 days. 0.1 ml A. hydrophila (108 cfu/ml) was injected to Tilapia. The result of the research on antibacterial shoes the wides clear zone at 1000 ppm with constipation, which is 9.7625 mm and the effectiveness test of red galangal extract on tilapia infected by A. hydrophila bacteria experience (ulcer) healing after 7 days with the concentration of rhizome extract of galangal 1000 ppm. Key words: red galangal rhizome extract, A. hydrophila, A. purpurata.

Phenolics from the Rhizomes of Kaempferia galanga L. and Their Antioxidant Activity

Phenolics from the Rhizomes of Kaempferia galanga L. and Their Antioxidant Activity

Phytochemical profiles, and antimicrobial and antioxidant activities of greater galangal [Alpinia galanga (Linn.) Swartz.] flowers

Previous studies of galangal (Alpinia galanga) have focussed mostly on rhizomes but seldom on flowers. A comprehensive look at galangal flowers could reveal additional benefits. The chemical composition of galangal flowers was significantly (p < 0.05) different from that of galangal rhizome. Pentadecane and α-humulene were identified as major compounds of galangal flower essential oil. 1′-acetoxyeugenol acetate was identified as the highest compound in flowers and exhibited the strongest antimicrobial activity among all fractions, with MIC50 values of 34 μg/ml against Staphylococcus aureus and 68 μg/ml against Listeria monocytogenes. Galangal flowers had a 3-fold higher total phenols content than had rhizomes (10.5 vs. 3.33 mg GAE/g powder). The antioxidant activities of different flower fractions varied from 2 to 4.45 mmol trolox/g phenolics. These findings suggest that antimicrobial and antioxidant agents extracted from galangal flowers could potentially be utilized as natural food preservatives or as therapeutic agents.

Isolation of Alkaloid Compounds from Ethanol Extract of Rimpang Galang Merah (Alpinia purpurata (Vielli) K. Schum) and nanoparticle production from its Alkaloid Extract. Comparative Study of Antibacterial Properties on Staphylococcus aureus and Eschericia

Isolation of alkaloid compounds from ethanol extract of red galangal rhizomes to compare their antibacterial properties with extracts formed into nanoparticles has been performed. The isolation of the alkaloid compounds was performed successively by salting using (i) hydrochloric acid (HCl) to pH 3 (when in ethanol) and using (ii) ammonium hydroxide (NH4OH) to pH 10 (when in chloroform). The alkaloid concentrated extract was obtained after chloroform was evaporated using a rotary evaporator. This alkaloid extract was then carried out by TLC silica gel GF254 using chloroform eluent: ethyl acetate (9: 2) to obtain a specific alkaloid type as a single/pure compound. Test of alkaloid purity successfully isolated by one-dimensional or two-dimensional TLC using various types of eluents with varying degrees of polarity, ie acetone, chloroform, ethyl acetate, ethanol and chloroform: ethyl acetate mixture (9: 2). Isolates of pure alkaloids were analyzed using UV-Vis spectrophotometers), FTIR and (LC-MS). The obtained alkaloid isolates were isokuinolin group with UV-visible absorption characteristics at maximum wavelength (λmaks) of 212 nm, 227 nm and 261 nm. The alkaloid isolate also showed the presence of functional groups -OH, -C-H, C = O, C = C, C = N, C-N, and C-O with molecular weight of 262.90 g/mol. The production of ethanol extract nanoparticles containing alkaloid-containing red galangal (EEAlkNPs) was performed using a top-down approach using ultrason (40 kHz, 2x50 watt) for 10 minutes. The EEAlkNPs size was determined by the dynamic light scattering method (DLS) using the Particles size analyzer (PSA) tool and obtained an average particle size of 220.2 nm. The antibacterial properties of EEAlkNPs were tested by disc diffusion method for the culture of S.aureus and E.coli bacteria. It is known that the antibacterial properties of EEAlkNPs are relatively larger than the original ethanol extract at the same concentration, ie, 2000 parts per million (bpj) or g / L.

Efek Pencampuran Bahan Pestisida Nabati Terhadap Keefektifannya Dalam Menekan Colletotrichum sp. In Vitro Serta Penyakit Antraknosa Pada Stroberi

Antrachnose disease caused by Colletotrichum sp. is one of the important diseases of strawberry in the field or after postharvest. The environmentally-friendly control measures is the use of botanical pesticides. Botanical pesticides that are commonly used by the farmers are mixture of several kinds of plant extract. This paper discuses the effects of combining water extract of garlic, galangal rhizome, and betel leaves on their efficacy in inhibiting conidial germination of Colletotrichum sp., the growth of miselium in vitro and the development of antracnose disease in strawberry. Preliminary study was conducted to determine the concentration of the extract that was used for further experiment. The experiment was arranged in the completely randomized design with 10 treatments involving water extract of garlic, galangal rhizome, betel leaves and their mixtures, fungicide and control/check. Each treatment was repeated three times.The results showed that combination of water extract of garlic, galangal rhizome and betel leaves did not enhance their efficacy. The inhibition effect of garlic water extract (5% concentration) was relatively better than its mixtures with galangal and betel leaves (total concentration 15%). The effects of galangal rhizome or betel leaves water extract and their mixtures were not significantly different. Water extract of garlic (15 %) inhibited Colletotrichum sp. which was showed by inhibition zone of 9.3 mm width; and suppressed the antrachnose disease in strawberry stolon by 83,7 %.

T47D cell-inhibiting indonesian medicinal plants and active constituents of Alpinia galanga rhizome

Background: The screening of Indonesian medicinal plant extracts against T47D cell line and identifying active chemical constituents of Alpinia galanga rhizome were conducted. Materials and Methods: Thirty methanol extracts of Indonesian medicinal plants were screened for possible anti-breast cancer. The T47D cell line was used as a target model. Chromatography techniques were used to purify the chemical constituents of A. galanga rhizome. Structural elucidation of isolated compounds was conducted by means of 1D and 2 D NMR (nuclear magnetic resonance) spectrometry. Their activities were also examined on MCF7, WiDr, and HeLa cell lines. Results: Five samples (A. galanga, Clonorchis sinensis, Piper cubeba, Santalum album, and Vitex trifolia) showed a strong activity with 96.4%, 91.9%, 87.6%, 82.6%, and 88.7% inhibition, respectively. Since A. galanga exhibited the most potent activity, its IC50value was determined with a dose-dependent effect with the IC50value of 21.2 μg/mL. Its methanol extract was also separated based on chromatography techniques producing the following four compounds: trans-p-coumaryl alcohol (1); p-coumaryl acetate (2); [1'S]-1'-acetoxy chavicol (3); and trans-p-coumaryl diacetate (4). Compounds 3 and 4 showed significant activity with the IC50values of 17.3 and 25.4 μg/mL, respectively. On the other hand, compounds 1 and 2 showed weak activity. All compounds exhibited a similar feature against MCF7, WiDr, and HeLa cell lines. Conclusions: On the structure-activity relationship observation, acetoxy group at a para position could be a key contributor to the effect. Thus, an acetoxy phenylpropanoid could be a good model for future anti-breast cancer lead compound development. Furthermore, extract plants should have demonstrated their potential to inhibit cancer cells. Abbreviations used: TLC: Thin layer chromatography, RPMI: Roswell Park Memorial Institute, NMR: Nuclear magnetic resonance, HMBC: Heteronuclear multiple bond correlations.

Daya Hambat Ekstrak Daun Sirsak, Kirinyuh, dan Rimpang Lengkuas terhadap Pertumbuhan Koloni Colletotrichum acutatum

Several plants that are potentially used as bio-pesticides are soursop, siam weed, and galangal. The research objective was to find out the effectiveness of leaf extract of soursop and C. odorata, and extract of galangal rhizome in inhibiting the growth of Colletotrichum acutatum colonies, causing antracnose on chilli, in in vitro. The experiment was conducted in the laboratory of Agriculture Faculty, Universitas Siliwangi Tasikmalaya from July until August 2016. The research design used was a completely randomized design consisted of nine treatments and three replications. The treatments were A ( control); B (soursop leaf extract, 0,5%); C (soursop leaf extract, 1%); D (C. odorata leaf extract, 0,5 %); E (C. odorata leaf extract 1%); F (galangal rhizome extract 0,5%); G (galangal rhizome extract 1%); H (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 0,5%; and I (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 1%). The results showed that the mixture of each 1% soursop leaf extract, C.odorata leaf extract and galangal rhizome extract was effective in inhibiting the growth of C. acutatum colonies at 7 and 14 days incubation period by 66,19% and 69,94% respectively. The three extracts are potentials as anti-fungus of C. acutatum.

Application of Essential Oils from Galanga Rhizome in Edible Coating Carrageenan as Antibacterial on Tilapia Fishball

Fish ball is a perishable food product. One of an alternative method which could be applied to delay the deterioration process was using carrageenan edible coating with the addition of galangal rhizome’s essential oil as an antibacterial agent. The aim of this study was to find out whether the addition of carrageenan edible coating with galangal rhizome’s essential oil could increase the shelf life of the tilapia fish ball during cold storage or not. This study was used experimental laboratories with the completely randomized design used factorial (2x6) two factors include essential oils concentrations (0%, dan 1%) and storage time (0, 3, 6, 9, 12, 15) with three repetitions. The shelf life of the fish ball observed by sensory, TPC, TVBN, pH, Aw, moisture content, and gel strength test. The parametric data were analyzed by the ANOVA, while the non-parametric was using the Kruskal Wallis test. As the result, the fish ball with the 1% treatment was still acceptable until 15 days of storage compared with the control which only lasts for 9 days. The other results were the sensory test was 6.63≤π≤6.95, TPC value was 1.07x105 cfu/g, TVBN value was 30.33±1.68 mgN%, the moisture content was 61.38%±0.47, the Aw was 0.89±0.006, the pH was 6.24±0.0, and the gel strength was 721.19±1.61 g.cm. The treatment of 1% essential oil addition to carrageenan edible coating was significantly different (p&amp;lt;0.05) compared to the control treatment during cold storage to the characteristic of tilapia fish ball. &lt;br /&gt;&lt;br /&gt;

OPTIMATION OF ETHANOL CONCENTRATION IN EXTRACTION OF EUGENOL FROM GALANGAL RHIZOME

Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F254 as stationary phase and applying a wavelength of 283 nm.Results: The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.Conclusion: Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.