Abstract
Several plants that are potentially used as bio-pesticides are soursop, siam weed, and galangal. The research objective was to find out the effectiveness of leaf extract of soursop and C. odorata, and extract of galangal rhizome in inhibiting the growth of Colletotrichum acutatum colonies, causing antracnose on chilli, in in vitro. The experiment was conducted in the laboratory of Agriculture Faculty, Universitas Siliwangi Tasikmalaya from July until August 2016. The research design used was a completely randomized design consisted of nine treatments and three replications. The treatments were A ( control); B (soursop leaf extract, 0,5%); C (soursop leaf extract, 1%); D (C. odorata leaf extract, 0,5 %); E (C. odorata leaf extract 1%); F (galangal rhizome extract 0,5%); G (galangal rhizome extract 1%); H (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 0,5%; and I (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 1%). The results showed that the mixture of each 1% soursop leaf extract, C.odorata leaf extract and galangal rhizome extract was effective in inhibiting the growth of C. acutatum colonies at 7 and 14 days incubation period by 66,19% and 69,94% respectively. The three extracts are potentials as anti-fungus of C. acutatum.
Highlights
S showed that the mixture of each 1% soursop leaf extract, C.odorata leaf extract and galangal rhizome extract was effective in inhibiting the growth of C. acutatum colonies at 7 and 14 days incubation period by 66,19% and 69,94% respectively
Several plants that are potentially used as bio-pesticides are soursop
The research objective was to find out the effectiveness of leaf extract
Summary
Percobaan ini dilaksanakan di Laboratorium Fakultas Pertanian Universitas Siliwangi Tasikmalaya yang berlangsung dari bulan Juli sampai Agustus tahun 2016. C. Penyediaan Media Potato Dextrose Agar (PDA) Pembuatan media tanam diawali dengan membuat larutan stok yang akan digunakan untuk uji efikasi pestisida nabati pada cawan petri. Pembuatan larutan stok dilakukan dengan melarutkan bahan PDA dalam aquadest sebanyak 39 g L-1 dan dipanaskan selama 15 menit, setelah PDA larut dimasukan pada erlenmeyer lalu disterilkan dalam autoklaf, kemudian PDA dicampur dengan pestisida nabati sesuai perlakuan lalu dimasukan ke dalam cawan petri dan didiamkan selama 24 jam. Uji efikasi berbagai ekstrak tumbuhan yang diujikan terhadap C. acutatum, yaitu dengan menumbuhkan isolat jamur C. acutatum pada media yang telah ditambahkan dengan masing-masing larutan ekstrak tumbuhan yang telah diuji pada perlakuan cawan petri. Acutatum dan biakan diinkubasikan pada suhu kamar Pengamatan dilakukan dengan cara menggambar pola koloni pada petridish terhadap mika plastik yang kemudian diukur bobotnya sesuai pola tersebut. Bila terdapat pengaruh perlakuan dilanjutkan dengan Uji Least Square Different (LSD) pada taraf 5%
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