Identification Of Functional Groups Research Articles

Exploring the cellular surface polysaccharide and root nodule symbiosis characteristics of the rpoN mutants of Bradyrhizobium sp. DOA9 using synchrotron-based Fourier transform infrared microspectroscopy in conjunction with X-ray absorption spectroscopy.

The functional significance of rpoN genes that encode two sigma factors in the Bradyrhizobium sp. strain DOA9 has been reported to affect colony formation, root nodulation characteristics, and symbiotic interactions with Aeschynomene americana. rpoN mutant strains are defective in cellular surface polysaccharide (CSP) production compared with the wild-type (WT) strain, and they accordingly exhibit smaller colonies and diminished symbiotic effectiveness. To gain deeper insights into the changes in CSP composition and the nodules of rpoN mutants, we employed synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy and X-ray absorption spectroscopy. FTIR analysis of the CSP revealed the absence of specific components in the rpoN mutants, including lipids, carboxylic groups, polysaccharide-pyranose rings, and β-galactopyranosyl residues. Nodules formed by DOA9WT exhibited a uniform distribution of lipids, proteins, and carbohydrates; mutant strains, particularly DOA9∆rpoNp:ΩrpoNc, exhibited decreased distribution uniformity and a lower concentration of C=O groups. Furthermore, Fe K-edge X-ray absorption near-edge structure and extended X-ray absorption fine structure analyses revealed deficiencies in the nitrogenase enzyme in the nodules of DOA9∆rpoNc and DOA9∆rpoNp:ΩrpoNc mutants; nodules from DOA9WT and DOA9∆rpoNp exhibited both leghemoglobin and the nitrogenase enzyme. IMPORTANCE This work provides valuable insights into how two rpoN genes affect the composition of cellular surface polysaccharides (CSPs) in Bradyrhizobium sp., which subsequently dictates root nodule chemical characteristics and nitrogenase production. We used advanced synchrotron methods, including synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy and X-ray absorption spectroscopy (XAS), for the first time in this field to analyze CSP components and reveal the biochemical changes occurring within nodules. These cutting-edge techniques confer significant advantages by providing detailed molecular information, enabling the identification of specific functional groups, chemical bonds, and biomolecule changes. This research not only contributes to our understanding of plant-microbe interactions but also establishes a foundation for future investigations and potential applications in this field. The combined use of the synchrotron-based FTIR and XAS techniques represents a significant advancement in facilitating a comprehensive exploration of bacterial CSPs and their implications in plant-microbe interactions.

Chemical Synthesis of Sulphur Nanoparticles and Their Characterization

This study focuses on the synthesis and comprehensive characterization of sulphur nanoparticles (S-NPs) using a precipitation method. The synthesis involved sodium thiosulphate and cetyl trimethyl ammonium bromide surfactants in a concentrated hydrochloric acid medium. The resulting S-NPs were thoroughly characterized using various advanced techniques. X-ray diffraction (XRD) analysis confirmed the crystalline nature of the nanoparticles, revealing distinctive diffraction patterns. Transmission electron microscopy (TEM) provided high-resolution images that elucidated the size and morphological aspects of the S-NPs, which exhibited a uniform size distribution. Scanning electron microscopy (SEM) further supported the morphological information, showcasing the surface features of the nanoparticles. Additionally, Fourier-transform infrared spectroscopy (FT-IR) analysis enabled the identification of functional groups and surface chemistry changes associated with the S-NPs. The comprehensive utilization of XRD, TEM, SEM, and FT-IR analysis provides a detailed understanding of the structural, morphological, and chemical attributes of the synthesized sulphur nanoparticles, paving the way for their potential applications in various fields.

Fabrication of B–CuO nanostructure and B -CuO/rGO binary nanocomposite: A comparative study in the context of photodegradation and antimicrobial activity assessment

This paper reports the synthesis of a cost-effective, stable, and reproducible Boron-doped copper oxide (B–CuO)/Reduced graphene oxide (rGO) binary nanocomposite. A conventional wet chemical synthetic route was used to fabricate the binary nanocomposite. X-ray diffraction (XRD) studies were performed to check the phase purity and crystallinity, scanning electron microscopic (SEM) technique for structural and morphological analysis, energy dispersive spectroscopy (EDX) for surface composition, and Fourier transform infrared spectroscopy (FT-IR) for functional group identification. The experimental results confirmed the formation of the pure nanocomposite. The photocatalytic activity of B–CuO and B–CuO/rGO binary nanocomposite was tested for Methylene blue (MB) dye degradation under sunlight. The B–CuO/rGO binary nanocomposite exhibited improved photocatalytic competence (98%) as compared to B–CuO (85%) in 90 minutes. The antimicrobial activity was measured against four bacterial strains viz. Escherichia coli (E.Coli), Bacillus amyloliquefaciens (B. amyloliquifaciens), Pseudomonas putida (P. putida), Bacillus subtilis (B. subtilis) and one fungal strains Rhizoctonia solani (R. solani). The minimum inhibitory concentration (MIC) was also calculated and it was found that B–CuO/rGO binary nanocomposite exhibited an excellent MIC of 62.2 μg/mL, against the gram-negative E. Coli.

Functional Group Identification and Toxicity of Piper crocatum Red Betel Acetone Extract against Artemia salina by Brine Shrimp Lethality Test Method

Identification of compounds and acetone extract activity from P. crocatum red shrimp has been done using phytochemical testing and Brine Shrimp Lethality Test (BSLT). The extraction begins with maseration using an acetone solvent and then the solvent is moved using an evaporator. Identification of extract function groups using the IR and GC-MS spectrum, as well as the toxicity test of the extract was carried out against the 48-hour Artemia salina Leach shrimp larva. The identification results showed the presence of flavonoids, phenylpropanoids, and steroids supported by GC-MS data and IR spectrum that indicated the existence of OH groups, C=O esters, C=O ketones, C=C olephins, C=C aromatic, C-O ester, and aliphatic CH. The results of probit analysis (LC50) showed that the acetone extract was highly toxic (LC50 0-100 μg/mL) to Artemia salina Leach with LC50 value of 2.4520 ppm.

CD44 tagged hyaluronic acid - chitosan liposome carrier for the delivery of berberine and doxorubicin into lung cancer cells

Liposomes are unique biomolecular, capable of loading both hydrophilic and hydrophobic molecules and delivered into the biological system. Liposomes (L) coated with hyaluronic acid (HA) and chitosan (CS) carrier system was fabricated. Berberine (BER) and doxorubicin (DOX) were encapsulated to enhance drug proliferation and therapeutic effect in lung cancer cells. The FTIR, XRD, SEM, and TEM techniques were carried out for functional group identification, crystallinity, and surface morphology analysis, respectively. In-vitro drug release confirms the sustained release of BER and DOX in various physiological environments. HA-CS@BER&DOX-L has good penetration ability and higher cytotoxicity effect in the A549 cells, and the IC50 value of HA-CS@BER&DOX-L is 89.19 μg/300 μL. The pure liposome showed a negligible cytotoxicity effect, and the HA-CS@BER&DOX-L could efficiently induce the apoptosis of A549 cells. The cellular uptake analysis of the HA-CS@BER&DOX-L effectively targeted and entered the A549 cells and clearly observed C. elegans images. Hence, the proposed system will be a potential treatment methodology to enhance the cytotoxicity of the A549 cancer cells and be useful to future drug administration methodology development.

SENSORY, GC-MS AND FTIR ANALYSIS OF AQUEOUS EXTRACT OF HIBISCUS SABDARRIFFA AND VERNONIA AMYGDALINA HERBAL TEA WITH BLENDS OF GINGER AND LEMON ZEST

The use of herbal remedies, especially in the form of teas for management of various diseases is gaining increasing popularity, making them the main stay of health care system, especially among the rural populace in the developing countries. Therefore, it is imperative to carryout proper analysis and screening of these plants, such as Hibiscus Sabdarriffa and Vernonia Amygdalina, to investigate their informed use. This research aimed at producing herbal teas from leaves of Hibiscus Sabdarriffa and Vernonia Amygdalina with additives of ginger and lemon zest with acceptable sensory attributes and also to investigate the phytocomponents of these herbal teas produced using GC-MS spectroscopy and FTIR spectroscopy. Ginger, lemon zest and fresh leaves of Hibiscus Sabdarriffa and Vernonia Amygdalina were collected from modern market areas of makurdi Benue State, authenticated by a plant taxonomist in Department of Botany, University of Agriculture Makurdi. Aqueous extracts of the herbal teas were analyzed using GC-MS Clarus 500 Perkin Elmer system. Identification of functional groups of samples (was done using FT-IR spectroscope (Shimadzu, IR Affinity 1, Japan). Results: FT-IR analysis of the herbal tea samples revealed the presence of phytochemicals like alkanes, alkenes, Carboxylic acids, phenols, alcohols, flavonoids, aromatics and saturated aliphatic esters,. GC-MS analyses of aqueous extract of the samples provided different peaks determining the presence of twenty two compounds with highest abundance. Twelve compounds were identified for Vernonia Amygdalina herbal tea sample and ten for Hibiscus Sabdarriffa herbal tea sample. The findings from this research showed that herbal teas produced from leave of Hibiscus Sabdarriffa and Vernonia Amygdalina had acceptable sensory attributes and also contains bioactive constituents of pharmacological importance for management of diseases which were identified by GC-MS and FTIR analysis.

Extraction and characterization of a newly developed cellulose enriched sustainable natural fiber from the epidermis of Mikania micrantha

Riding on the journey of a sustainable world it is very crucial to extend the usage of natural cellulosic fiber from renewable sources. Due to their numerous applications and eco-friendly behavior, natural cellulosic fibers are in greater demand every day. In this article a new natural fiber extracted from the creepers of Mikania micrantha with the help of 5% NaOH retting process. Previously no research work have been done with this fiber. The fiber was characterized by following ASTM D1909, ASTM D 2654, ASTM D1445, TAPPI standard for determination of moisture regain and content, bundle fiber strength and chemical composition respectively. XRD, SEM, FTIR and TGA analysis were also done for the identification of crystallinity, fiber morphology, functional group and thermal behavior. The tests results showed that it is a cellulose enriched textile fiber having 56.42% cellulose. The average moisture regain and content % were 9.17% and 8.4% respectively analyzed from the five samples. The average tenacity was determined 38.6 gm/tex with 1.8% elongation and the crystallinity of the tested fiber was 72%. The maximum degradation temperature for this fiber was 477 °C. The application of this noble fiber can be for making fiber reinforced composites, cellulose nanomaterials, biomaterials etc.

Adsorptive Removal of Dye (Methylene Blue) Organic Pollutant from Water by Pine Tree Leaf Biomass Adsorbent

In this laboratory batch adsorption study, the raw pine tree leaf biomass solid waste adsorbent material was used for the removal of methylene blue (MB) dye from water at different physicochemical process conditions. The characteristics of adsorbents were determined for particle size, surface area, the existence of functional group identification, and the morphology of the adsorbent surface. The adsorption was performed at different process conditions, which include solution pH, dye concentrations, adsorbent doses, and temperature, respectively. In this study, it was found that MB dye adsorption increased with increases in solution pH and adsorbate MB dye concentration but decreased with adsorbent doses and temperature at fixed process conditions. The Langmuir isotherm model was best fitted with the experimental equilibrium data, with a higher linear regression coefficient (R2) value of 99.9% among the two widely used Langmuir and Freundlich adsorption isotherm model equations. The maximum Langmuir monolayer adsorption capacity of raw pine leaf was found to be 36.88 mg/g, which was comparable with other reported adsorbent capacities towards methylene blue (MB) dye adsorption. The value of the separation factor, RL, from the Langmuir isotherm model equation gives an indication of favorable adsorption. Thermodynamic parameters such as standard Gibbs free energy change (ΔG0), standard enthalpy change (ΔH0), and standard entropy change (ΔS0) indicated that the methylene blue (MB) dye adsorption by pine tree leaf biomass was spontaneous and exothermic in nature and that the mechanism of adsorption was mainly physical adsorption. Finally, limitations and future studies are also discussed here. The outcome of this batch adsorption study may result in the valorization of locally available large pine tree leaf residue waste, which could be used in water purification.

Photocatalytic Degradation of Methylene Blue Dye from Wastewater by Using Doped Zinc Oxide Nanoparticles

ZnO is a semiconductor material that has important physical and chemical properties, which are frequently and significantly enhanced by the addition of impurities, such as doping. A study of the structural properties of pristine and functionalized (i.e., doped with Antimony and Tungsten) ZnO nanoparticles has been conducted for the photocatalyst-based degradation of methylene blue (MB) dye under both Ultraviolet (UV) and solar light. Authors have used a 1% concentration of dopant for doping purposes. The synthesized materials were characterized for structural analysis, functional group identification, spectroscopic measurements, and morphological examination using X-ray diffraction (XRD), Fourier transform-infrared (FTIR), UV-Vis spectroscopy (UV-Vis), and Field emission scanning electron microscope (FESEM) techniques. XRD analysis confirmed that the synthesized-doped materials retained the wurtzite hexagonal structure with a purity of 99%. Transmission electron microscope (TEM) analysis data reveals the average size of pure ZnO-NPs was found to be 7 nm; after doping the size was found to be increased to 18 nm and 9.55 nm, respectively, for ZnO-W and ZnO-Sb. As per FESEM analysis results, minor morphological changes were observed after doping. The Ultraviolet Differential reflectance spectroscopy UV-DRS study revealed the confirmation of ZnO doping with antimony and tungsten, which exhibited a blue shift. The decrease in the band-gap on doping makes the ZnO-NPs more efficient for photocatalytic applications. The photocatalytic efficiency of pristine and doped ZnO-NPs catalysts for methylene blue photocatalytic degradation (PCD) was analyzed under both UV and solar irradiation. This study analyzed the effect of pH, nano-photocatalyst dose, and initial dye concentration (ICD) on the PCD of MB. The obtained analytical results showed that the ideal conditions for the PCD of MB dye are as follows: pH = 9, the quantity of the nano-photocatalyst used was 300 mg/L, and an initial MB dye dose of 10 ppm. These conditions lead to a PCD of about 91% of the MB dye by using ZnO-Sb nano-photocatalyst on exposure to solar radiation. The reusability study also revealed the stability of nano-photocatalysts. The current research may pave the way for the removal of hazardous dyes from wastewater discharged by many industries.

Successful preparation of tercopolymer hydrogel for the removal of toluidine dye from contaminated water: Experimental and DFT study

ABSTRACT Polymeric hydrogel with pH-sensitive surface was prepared in this study. The surface morphologies of the synthesized materials were examined using scanning electron microscopy. Surface area and pore sizes of the hydrogels were determined using Brunauer–Emmett–Teller. Fourier transform infra-red spectroscopy was used for the identification of various functional groups on the surface of these adsorbents. These hydrogels were extremely efficient in removing toxic toluidine dye from contaminated water. Kinetics and isothermal models were used for the calculations of adsorption parameters of the synthesized materials. The adsorption data were impeccably explained with pseudo‐second order and the Langmuir model as compared to other models. The spontaneity of the reaction was confirmed by thermodynamic study. In this study the value of enthalpy (∆H°) was 7322.6 kJ/mol. The values of Gibbs energy (∆G°) were −453.9, −984.4 and −1515.2 kJ/mol, at 293K, 313K and 333K, respectively, for toluidine dye, which showed that the process is endothermic and spontaneous in nature. Moreover, the value for entropy (∆S°) was calculated as 26.54 Jmol/K for toluidine dye. The interactions of various functional groups on the surface of adsorbent and toluidine dye molecules were studied with density functional theory simulations. The large binding energies (−5.73 to −25/74 kcal/mol) of different complexes showed that the hydrogels had strong affinity for the adsorption of toluidine dye molecules while the negative values revealed that the process is spontaneous and endothermic. These results were consistent with the experimental results. This study revealed that the synthesized hydrogel is efficient adsorbent material for toluidine dye removal from contaminated water. The adsorption capacity of the prepared hydrogel was 526 mg/g for toluidine dye.

Optimization of solvent evaporation method in liposomal nanocarriers loaded-garlic essential oil (Allium sativum): Based on the encapsulation efficiency, antioxidant capacity, and instability.

This study is aimed to optimise the preparation factors, such as sonication time (5-20min), cholesterol to lecetin ratio (CHLR) (0.2-0.8), and essential oil content (0.1-0.3g/100g) in solvent evaporation method for formulation of liposomal nanocarriers containing garlic essential oil (GEO) in order to find the highest encapsulation efficiency and stability with strongest antioxidant and antimicrobial activity. The droplet size, zeta potential, encapsulation efficiency, turbidity, changes in turbidity after storage (as a measure of instability), antioxidant capacity, and antimicrobial activity were measured for all prepared samples of nanoliposome. The sonication time is recognised as the most effective factor on the droplet size, zeta potential, encapsulation efficiency, turbidity, and instability while CHLR was the most effective factor on zeta potential and instability. The content of GEO significantly affected the antioxidant and antimicrobial activity in particular against gram-negative bacteria (Escherichia coli). The results of FTIR based on the identification of functional groups confirmed the presence of GEO in the spectra of the prepared nanoliposome and also it was not observed the interaction between the components of the nanoliposome. The overall optimum conditions were determined by response surface methodology (RSM) as the predicted values of the studied factors (sonication time: 18.99min, CHLR: 0.59 and content of GEO: 0.3g/100g) based on obtaining the highest stability and efficiency as well as strongest antioxidant and antimicrobial activity.

CD44 targeted delivery of oncolytic Newcastle disease virus encapsulated in thiolated chitosan for sustained release in cervical cancer: a targeted immunotherapy approach.

Cervical cancer accounts for one of most common cancers among women of reproductive age. Oncolytic virotherapy has emerged as a promising immunotherapy modality but it comes with several drawbacks that include rapid clearance of virus from body due to immune-neutralization of virus in host. To overcome this, we encapsulated oncolytic Newcastle disease virus (NDV) in polymeric thiolated chitosan nanoparticles. For active targeting of virus loaded nanoformulation against CD44 (cluster of differentiation 44) receptors which are overly expressed on cancer cells, these nanoparticles were surface functionalized with hyaluronic acid (HA). Using half dose of NDV (TCID50 (50% tissue culture infective dose) single dose 3 × 105), virus loaded nanoparticles were prepared by green synthesis approach through ionotropic gelation method. Zeta analysis was performed to analyse size and charge on nanoparticles. Nanoparticles (NPs) shape and size were analysed by SEM (scanning electron microscope) and TEM (transmission electron microscope) while functional group identification was done by FTIR (fourier transform infrared) and XRD (X-ray diffraction). Viral quantification was done by TCID50 and Multiplicity of infection (MOI) determination while oncolytic potential of NPs encapsulated virus was analysed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and cell morphology analysis. Zeta analysis showed that average size of NDV loaded thiolated chitosan nanoparticles surface functionalized with HA (HA-ThCs-NDV) was 290.4nm with zeta potential of 22.3 mV and 0.265 PDI (polydispersity index). SEM and TEM analysis showed smooth surface and spherical features of nanoparticles. FTIR and XRD confirmed the presence of characteristic functional groups and successful encapsulation of the virus. In vitro release showed continuous but sustained release of NDV for up to 48 hours. TCID50 for HA-ThCs-NDV nanoparticles was 2.63x 106/mL titter and the nanoformulation exhibited high oncolytic potential in cell morphology analysis and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay as compared to naked virus, in dose dependent manner. These findings suggest that virus encapsulation in thiolated chitosan nanoparticles and surface functionalization with HA is not only helpful in achieving active targeting while masking virus from immune system but, it also gives sustained release of virus in tumor microenvironment for longer period of time that increases bioavailability of virus.

Functionalized hexagonal boron nitride nanoplatelets for advanced cementitious nanocomposites

Portland cement-based nanocomposites were successfully fabricated with low volume fractions of hexagonal boron nitride (hBN) nanoplatelets, exfoliated and functionalized using a combination of ball milling and sonicated-assisted dispersion. Surface topography, thickness, lateral dimension, and number of layers of the hBN nanoplatelets were evaluated by AFM and Raman analysis. The identification of functional groups attached onto the functionalized hBN was performed through FTIR. The results show that the functionalization successfully exfoliates the hBN nanoplatelets to few-layer thicknesses, resulting in suspensions with high colloidal stability. The grafted hydroxyl and carboxyl groups on the hBN surface interact with the Ca2+ ions of calcium silicate hydrates (CSH), improving the load-transfer efficiency from the cement matrix to the hBN nanoplatelets. Overall, the hBN reinforced cementitious composites demonstrated significant enhancement in flexural strength by ∼50%, compressive strength by ∼17%, Young's modulus by ∼56%, and fracture energy by ∼76%.

Characterization and Identification of Chemical Fungtional Groups in Oyster Mushroom (Pleurotus Ostreatus) Broth with edded Vegetables and Spices

Adding the nutritional content of broth powder without destroying the distinctive taste of the broth can be done using pure mushroom extract and additional vegetables and spices. This study aims to determine the organoleptic characteristics and identify the chemical functional groups of glutamic acid in the best samples of oyster mushroom broth powder added with vegetables and spices. The results of the organoleptic test were tabulated in a table and then analyzed using a descriptive test. The organoleptic characteristics of the best color parameters were in treatment B1 which had a pale white color . The best treatment of aroma, texture, and taste parameters were found in the B2 treatment with a characteristic aroma of typical broth, fine powder texture, and has a distinctive taste of broth. The identification of the functional groups of secondary metabolites using the Fourier Transform Infrared (FTIR) instrumentation. Identification results with FTIR showed absorption at wave number (cm-1): 3419.74, 2934.62, 1639.56, 1409.70, 1055.55. There is N-H, O-H, C-H, C=O carbonyl group and carboxylic C-O, which identify the presence of amide compound, phenol acid, alkane, aldehydes, amid I and carboxylic acid, which are known as glutamic acid in this sample. Keywords: Organoleptic, Functional Groups Identification, Oyster Mushroom Broth Powder Why only B1 that you mentioned in color parameters?

Analisis Kadar Kurkumin pada Herbal Oil Kunyit Ekstrak Virgin Coconut Oil dengan Metode Ultrasonik dan Maserasi

Vegetable oil theoretically and experimentally has proven its potential as an alternative solvent in the extraction of natural materials. Turmeric infused with virgin coconut oil (VCO) is a way to take advantage of the active compounds from the herbs and the oil itself. This study aims to determine the ability of the VCO solvent in extracting curcumin compounds from turmeric with the addition of tween 80 surfactants. The extraction method used was maceration at 60℃ and an ultrasonic frequency of 40 kHz for 15 minutes. Thin layer chromatography (TLC) was used for qualitative analysis of each herbal oil extract using chloroform: methanol (95:5) eluent and FTIR spectrophotometer to identify functional groups. The highest curcumin content was found in herbal oil with tween 80 of 2 ml, namely 31 ppm in maceration extraction and 260.7 ppm in ultrasonic extraction. Identification of curcumin functional groups in herbal oils showed absorption patterns of O-H, C=O, aromatic C=C, C-O-C, and C-H.

Elucidating the chemical structures of petroleum resin using solid-state 13C NMR

In this study, a new method to perform the solid-state 13C NMR analysis of oil resin has been proposed and the 3D molecular model of oil resin has been established for the first time. This method is not only suitable for petroleum resin, but also can be used in the structural detection of all colloidal component of fossil fuel. In this research, both liquid- and solid-state 13C NMR based on CP-TOSS/MAS method have been carried out to detect the chemical structural of petroleum resin. Solid-state 13C NMR showed less influence from solvent peaks and achieved a more accurate identification of aromatic carbon and oxygen-containing functional groups than liquid-state 13C NMR, and the FT-IR was used to verify this; thus, it is more suitable for resin structure detection. Based on the results of the two types of NMR, the petroleum resin structure was found to consist of aromatic clusters at the core, with short aliphatic chains and cycloalkanes connected in the periphery. In addition, two new parameters were established to evaluate the length of aliphatic chains (CnR) and oxygen-containing in resins (OINMR: NMR oxygen index of resin). Combined with elemental analysis, the average 3D resin molecular model was established in this research. In general, this study underscored a suitable method for detecting chemical structures in the field of fossil fuel and energy engineering, and successfully introduced solid-state 13C NMR for the analysis of colloidal component of fuel. At the same time, the most stable structural model of petroleum resin in three-dimensional space was established; this provides a basic model for the molecular simulation research of the constituents of petroleum. Considering the important influence of resin on the properties of petroleum, this research has a great significance to migration and exploitation of heavy oil.

Modulating the characteristics of jackfruit seed starches by annealing and autoclaving‐cooling modifications

AbstractThis study investigated the effects of physical modification (annealing and autoclaving) on functional, structure and thermal properties of jackfruit seed starch. The amylose, amylopectin, morphological properties, functional group identification, crystalline behavior, viscosity and thermal properties of modified jackfruit seed starch were analyzed. Results showed that compared with the native starch, amylose content of annealed jackfruit seed starch increased (from 30.68% to 32.39%) while after autoclaving it was decreased (from 30.68% to 25.44%). There were no changes in the X‐ray diffraction patterns of annealed starches while in autoclaving‐cooling treatment, a new peak appeared, causing the crystalline change in structure of starch from A‐type to B‐type. DSC data revealed that thermal properties of jackfruit seed starches improved after both annealing and autoclaving‐cooling treatments. This paper can potentially be used as a theoretical reference to prompt more investigations for the extensive utilization of jackfruit seed starches in the food and other industries.Practical applicationsPhysically modified jackfruit seed starch with improved functionality makes the starch well suitable for various food applications due to changes in solubility, water absorption, and swelling ability. These modified starches can be potentially used during the preparation of noodles, doughs, baked products, pie‐fillings, soups, and sausages and will also remove the environmental burden and food waste.

Commiphora Wightii: A Natural Approach in Control of Urinary Tract Infections

Background: To investigate the extracts of Commiphora wightii for the presence of phytoconstituents and screen anti-microbial activity against dominant UTI pathogens. GC-MS and FTIR are used for the identification of functional groups of the bioactive compounds. Methods: Five different solvents were screened for extraction of compounds from the powdered plant at temperatures ranging from 60 to 80 upto 72 hours. These extracts were used for preliminary phytochemical and antimicrobial analyses. Functional groups were identified by using Fourier-Transform Infrared Spectrophotometer scanning from 450 to 4000 cm-1 and a 4 cm-1 resolution. The existence of the major compound with the most promising antimicrobial activities was discovered utilising GC-MS analysis. Result: The results revealed that the methanolic extract of Commiphora wightii displayed significant glycosides and flavonoids. Fifty-two functional compounds were identified using GC-MS and FTIR.

Fabrication of Zinc Ferrite (ZnFe<sub>2</sub>O<sub>4</sub>) by Sonochemical Methods: Physical and Magnetic Properties in Various Temperatures

This study aims to determine the magnetic properties and structural properties of zinc ferrite (ZnFe2O4) material with the sonochemichal synthesis method. The sonochemichal method was obtained by sonication lasting for 30 minutes with the addition of 100 ml of 10 M NaOH. ZnFe2O4 material was sintered with temperature variations of 950°C, 1050°C, and 1150°C with a holding time of 2 hours. Phase identification revealed that the cubic phase structure of zinc ferrite is franklinite and also obtained crystal size results with values ​​of 70.58 – 84.71 nm. Morphological identification revealed that the ZnFe2O4 material had an irregular cubic shape and the highest agglomeration was at ZnFe2O4 temperature of 950°C. Identification of functional groups using FTIR characterization resulted in the wavelength range of 400-600cm-1 having basic lattices of Fe-O and Zn-O which occupy tetrahedral and octahedral positions, respectively. Magnetic identification uses VSM characterization which results that the sample is softmagnetic and gets several Mr, Ms, and Hc values. ZnFe2O4 with a sintering temperature of 1150°C in this study has the potential to be used as a microwave device.

Bond Characterization in Cementitious Material Binders Using Fourier-Transform Infrared Spectroscopy

Fourier-transform infrared (FTIR) spectroscopy is a fast and simple technique for functional group identification. This work provides a review and insight into the application and interpretation of FTIR spectroscopy for cementitious binders that comprise ordinary Portland cement, alkaline-activated binders, geopolymers, and material characterization for civil engineering material applications. This technique can be used to identify different compounds and a moiety of bond vibrations in inorganic molecules such as Si-O, -OH, H-O-H (water), C-O (carbonate or carbonation), aluminosilicate (Si-O-T, where T is Al or Si), and S-O (sulfate or gypsum) found in hydrated cement, alkaline binders, and geopolymers. The prominent bands include those representing carbonation (CO32− 1390–1475 cm−1), calcium carbonate (871, 1792–2516 cm−1), hydroxylation and water molecules (1607, 3400–3650 cm−1), strength skeletal framework compositions or Al-Si substitutions, silicate organization (C-A-S-H, N-A-S-H, or C-S-H (950–1055 cm−1), and sulfate (600–680, 1080–1100 cm−1). Some of the factors that could affect the spectra bands include elemental displacement due to changes in molar mass, activated temperature, pH, activator concentration, w/b ratio, Ca/Si ratio, Si/Al ratio, and the silica modulus (SiO2/Na2O) of the activators used in the binder synthesis. The method could be used for destructive and non-destructive testing on paste sample by using transmission and attenuated total reflectance methods, respectively.