Full Factorial Design Research Articles

Optimizing wind-catching butterfly roofs to improve cross natural ventilation performance in apartment buildings

Abstract This study aims to enhance cross natural ventilation performance in multi-level double-loaded apartments by employing a wind-catching butterfly roof, specifically designed for apartments with a center-to-center opening configuration. A 2k full-factorial design of experiment (DOE) method with a center point is utilized for design optimization. Nine configurations, determined by roof height h and roof angle α, are assessed using Computational Fluid Dynamics (CFD). After the initial DOE assessment, three additional butterfly roof designs with varying α values are explored. The dimensionless flow rate (DFR) serves as the objective function for optimization. The optimal design features a butterfly roof with h = 0.01m and α = 30°, demonstrating improvements of 2.5%−4.9% in DFR and 5.3%−9.0% in the pressure coefficient C p compared to scenarios without a butterfly roof. Butterfly roofs also increased the velocity magnitudes in the rooms of the leeward block, with configurations h = 0.01m and α = 35° and 37.5° showing the most significant increases of 58.5% − 86.9% on levels 1 to 3. The discharge coefficient C d induced by all butterfly roof configurations are within 0.6 − 0.7 on levels 1 to 3, while the C d values for levels 4 to 6 are within 0.5 − 0.6. Although butterfly roofs can enhance indoor air quality, they require further research to meet all recommended guidelines. These findings provide valuable insights for architects and urban planners aiming to reduce the environmental footprint of apartment buildings. This research presents a comprehensive evaluation of the potential of butterfly roofs to enhance apartment ventilation and offers recommendations for future design improvements.

Optimizing Milling Parameters for Enhanced Machinability of 3D-Printed Materials: An Analysis of PLA, PETG, and Carbon-Fiber-Reinforced PETG

Fused deposition modeling (FDM) is widely applied in various fields due to its affordability and ease of use. However, it faces challenges such as achieving high surface quality, precise dimensional tolerance, and overcoming anisotropic mechanical properties. This review analyzes and compares the machinability of 3D-printed PLA, PETG, and carbon-fiber-reinforced PETG, focusing on surface roughness and burr formation. A Design of Experiments (DoE) with a full-factorial design was used, considering three factors: rotation speed, feed rate, and depth of cut. Each factor had different levels: rotational speed at 3000, 5500, and 8000 rpm; feed rate at 400, 600, and 800 mm/min; and depth of cut at 0.2, 0.4, 0.6, and 0.8 mm. Machinability was evaluated by roughness and burr height using a profilometer for all the materials under the same milling conditions. To evaluate the statistical significance of the influence of various processing parameters on surface roughness and burr formation in 3D-printed components made of three different materials—PLA, PETG, and carbon-fiber-reinforced PETG—an analysis of variance (ANOVA) test was conducted. This analysis investigated whether variations in rotational speed, feed rate, and depth of cut resulted in measurable and significant differences in machinability results. Results showed that milling parameters significantly affect roughness and burr formation, with optimal conditions for minimizing any misalignment highlighting the trade-offs in parameter selection. These results provide insights into the post-processing of FDM-printed materials with milling, indicating the need for a balanced approach to parameter selection based on application-specific requirements.

Differential effects of antidepressant sertraline in glochidia-fish interactions involving drug transfer from parasite to host

This study examined the impact of sertraline, an antidepressant common in treated wastewater, on the host-parasite dynamics between parasitic freshwater mussel (Unio tumidus, Unionidae) larvae (glochidia) and their host fish (Squalius cephalus, Cyprinidae). Employing a full-factorial design, both fish and glochidia were subjected to sertraline at the combinations of 0 µg L−1 (control), 0.2 µg L−1 (environmentally relevant concentration), and 4 µg L−1 (elevated concentration, short-term exposure of the parasite). The results showed that long-term host exposure (involving intensive sertraline accumulation in the fish brain) marginally increased subsequent glochidia attachment success by 2 %, while parasite exposure at the same environmentally relevant concentrations had no detectable effect. There was also no effect of exposure of glochidia to 0.2 µg L−1 of sertraline on their viability and encapsulation success during the initial parasitic stage. However, a significant alteration in attachment behavior, marked by a 3.3 % increase in attachment success and changes in the glochidia spatial distribution on the host body, was noted after 24 h of glochidia exposure to 4 µg L−1 of sertraline. Importantly, this study provides the first evidence of sertraline transfer from exposed glochidia to nonexposed host fish, as indicated by elevated levels of sertraline (12.8 ng g−1) in the brain tissue of nonexposed hosts. These findings highlight the subtle yet significant effects of pharmaceutical pollutants on freshwater ecosystems but also underscore the importance of understanding the unexpected dynamics of such contamination to predict and address future ecological changes.

Temperate trees locally engineer decomposition and litter-bound microbiomes through differential litter deposits and species-specific soil conditioning.

Leaf decomposition varies widely across temperate forests, shaped by factors like litter quality, climate, soil properties, and decomposers, but forest heterogeneity may mask local treeinfluences on decomposition and litter-associated microbiomes. We used a 24-yr-old common garden forest to quantify local soil conditioning impacts on decomposition and litter microbiology. We introduced leaf litter bags from 10 tree species (5 arbuscular mycorrhizal; 5 ectomycorrhizal) to soil plots conditioned by all 10 species in a full-factorial design. After 6 months, we assessed litter mass loss, C/N content, and bacterial and fungal composition. We hypothesized that (1) decomposition and litter-associated microbiome composition would be primarily shaped by the mycorrhizal type of litter-producing trees, but (2) modified significantly by underlying soil, based on mycorrhizal type of the conditioning trees. Decomposition and, to a lesser extent, litter-associated microbiome composition, were primarily influenced by the mycorrhizal type of litter-producing trees. Interestingly, however, underlying soils had a significant secondary influence, driven mainly by tree species, not mycorrhizal type. This secondary influence was strongest under trees from the Pinaceae. Temperate trees can locally influence underlying soil to alter decomposition and litter-associated microbiology. Understanding the strength of this effect will help predict biogeochemical responses to forest compositional change.

Microfluidics-on-a-chip for designing celecoxib-based amorphous solid dispersions: when the process shapes the product

AbstractThe fundamental idea underlying the use of amorphous solid dispersions (ASDs) is to make the most of the solubility advantage of the amorphous form of a drug. However, the drug stability becomes compromised due to the higher free energy and disorder of molecular packing in the amorphous phase, leading to crystallization. Polymers are used as a matrix to form a stable homogeneous amorphous system to overcome the stability concern. The present work aims to design ASD-based formulations under the umbrella of quality by design principles for improving oral drug bioavailability, using celecoxib (CXB) as a model drug. ASDs were prepared from selected polymers and tested both individually and in combinations, using various manufacturing techniques: high-shear homogenization, high-pressure homogenization, microfluidics-on-a-chip, and spray drying. The resulting dispersions were further optimized, resorting to a 32 full-factorial design, considering the drug:polymers ratio and the total solid content as variables. The formulated products were evaluated regarding analytical centrifugation and the influence of the different polymers on the intrinsic dissolution rate of the CXB-ASDs. Microfluidics-on-a-chip led to the amorphous status of the formulation. The in vitro evaluation demonstrated a remarkable 26-fold enhancement in the intrinsic dissolution rate, and the translation of this formulation into tablets as the final dosage form is consistent with the observed performance enhancement. These findings are supported by ex vivo assays, which exhibited a two-fold increase in permeability compared to pure CXB. This study tackles the bioavailability hurdles encountered with diverse active compounds, offering insights into the development of more effective drug delivery platforms. Graphical Abstract

Development of ergo-refined operator's workplace and biophysically actual cost-benefit analysis of riding type self-propelled machines with special reference for female operators.

Female agricultural workers contribute to 37% of the total agricultural workforce in India, however, most self-propelled machinery is designed for male agricultural workers. The primary objective was to determine the impact of the ergo-refined operator's workplace on various aspects of operator performance and comfort, including actuating force, posture, and physiological parameters. Experiments were carried out in real field conditions using a full factorial randomized design. Twelve female operators participated in the study, and measurements were taken for control lever actuating force, operator posture, heart rate, and other relevant parameters. The ergo-refined operator's workplace intervention resulted in significant reductions in actuating force for various control levers, angles of joints, working heart rate (WHR), oxygen consumption rate (OCR), muscle load, and whole-body vibration (WBV) acceleration. These reductions were observed under different operating conditions. The findings suggest that the ergo-refined operator's workplace is effective in enhancing operator comfort and reducing physical strain during the operation of riding type self-propelled machines. It contributes to improved safety, comfort, and operational efficiency for operators working in field conditions. ANOVA and MANOVA analyses confirmed the positive impact of operating conditions and engine speed on the measured parameters when using the ergo-refined operator's workplace.

Multiple stressors affecting microbial decomposer and litter decomposition in restored urban streams: Assessing effects of salinization, increased temperature, and reduced flow velocity in a field mesocosm experiment

A multitude of anthropogenic stressors impact biological communities and ecosystem processes in urban streams. Prominent among them are salinization, increased temperature, and altered flow regimes, all of which can affect microbial decomposer communities and litter decomposition, a fundamental ecosystem process in streams. Impairments caused by these stressors individually or in combination and recovery of communities and ecosystem processes after release from these stressors are not well understood. To improve our understanding of multiple stressors impacts we performed an outdoor stream mesocosm experiment with 64 experimental units to assess the response of microbial litter decomposers and decomposition. The three stressors we applied in a full-factorial design were increased salinity (NaCl addition, 0.53 mS cm−1 above ambient), elevated temperature (3.5 °C above ambient), and reduced flow velocity (3.5 vs 14.2 cm s−1). After two weeks of stressor exposure (first sampling) and two subsequent weeks of recovery (second sampling), we determined leaf-associated microbial respiration, fungal biomass, and the sporulation activity and community composition of aquatic hyphomycetes in addition to decomposition rates of black alder (Alnus glutinosa) leaves confined in fine-mesh litter bags. Microbial colonization of the litter was accompanied by significant mass loss in all mesocosms. However, there was little indication that mass loss, microbial respiration, fungal biomass, sporulation rate or community composition of aquatic hyphomycetes was strongly affected by either single stressors or their interactions. Two exceptions were temperature effects on sporulation and decomposition rate. Similarly, no notable differences among mesocosms were observed after the recovery phase. These results suggest that microbial decomposers and leaf litter decomposition are either barely impaired by exposure to the tested stressors at the levels applied in our experiment, or that communities in restored urban streams are well adapted to cope with these stressor levels.

Waste-Heat Renewable Gasifier Design through Taguchi's Method and MANFIS

Ensuring a global average temperature increase of no more than 1.5°C is crucial for aligning with the objectives of the Paris Agreement. The Intergovernmental Panel on Climate Change (IPCC) estimates that a 45% reduction in carbon dioxide emissions by 2030 or a 25% reduction by the same year is necessary to cap the temperature rise at 2°C. Failure to achieve this target by 2030 would necessitate substantial subsequent reductions to compensate for the delayed progress toward net zero emissions, potentially incurring higher costs. Elevating the focus on enhancing energy efficiency has emerged as a key strategy in expediting progress toward these climate goals. Hydrogen, renowned for its high efficiency and emission-free combustion, is anticipated to play a pivotal role in this endeavor. Traditionally, gasifiers have served as the conduit for transforming inexpensive fuels like coal or biomass into hydrogen. However, their energy-intensive nature poses a challenge.Addressing this inherent issue, an innovative approach known as the external-heating gasifier has been proposed and designed. The design of the external-heating gasifier represents a quintessential nonlinear and multiple-input multiple-output (MIMO) problem. To achieve optimal performance, the integration of Taguchi's method and inverse-model technology has been employed, with a simulation platform developed using the COMSOL Multiphysics software.Initially, Taguchi's method was utilized to determine the minimum number of experiments required for a full-factorial design and identify the most critical factors influencing product performance. Subsequently, an adaptive neuro-fuzzy inference system (ANFIS) was developed with a MIMO–ANFIS architecture. This system was employed to train an inverse model, facilitating the mapping of relationships between each input and output set. In essence, this approach enables the identification of manufacturing parameters that result in optimal performance. To illustrate the efficacy of the proposed method, a simulation study on an existing gasifier is presented.

Effect of pulsed electric field processing on the quality characteristics and enzyme activity of tender coconut water.

Tender coconut water (TCW) is a natural drink rich in natural electrolytes, minerals, salts and sugars; it has good health benefits. But, its shelf-life is very limited because of the active nature of enzymes present in it when exposed to air. Therefore, the processing of TCW is necessary to inactivate the enzymes. So, this study aims to observe the effect of various process parameters of pulsed electric field (PEF) on the quality parameters of TCW. For the treatment of TCW with PEF, a full-factorial design of experiments was followed with process parameters such as three levels of electric field intensity (8, 12, and 16 kV/cm), two levels of pulse width (PW) (50 and 70 μs), and six levels of the number of pulses (2000 to 12,000 pulses) were considered at a constant pulse OFF time of 75 ms. PEF treatment did not significantly change pH, total soluble solids, and viscosity. However, it significantly affected vitamin C, colour, and total and reducing sugars. PEF treatment significantly enhanced the total phenolic content and antioxidant activity by 23.17% and 42.49%, respectively. At the same time, significant inactivation of polyphenol oxidase (100%) and peroxidase (60.2%) was observed at PEF treatment conditions of 16 kV/cm, 70 μs PW, and 12,000 pulses. Moreover, no significant change in the sensory acceptability of PEF-treated TCW (16 kV/cm, 70 μs PW, 12,000 pulses) when compared to the untreated/fresh TCW, which is a promising sign.

Combined effects of three insecticides with different modes of action on biochemical responses of the solitary bee Osmia bicornis

Bees are simultaneously exposed to a variety of pesticides, which are often applied in mixtures and can cause lethal and sublethal effects. The combined effects of pesticides, however, are not measured in the current risk assessment schemes. Additionally, the sublethal effects of pesticides on a variety of physiological processes are poorly recognized in bees, especially in non-Apis solitary bees. In this study, we used a full-factorial design to examine the main and interactive effects of three insecticide formulations with different modes of action (Mospilan 20 SP, Sherpa 100 EC, and Dursban 480 EC) on bee biochemical processes. We measured acetylcholinesterase (AChE), glutathione S-transferase (GST) and esterase (EST) activities, as well as a nonenzymatic biomarker associated with energy metabolism, i.e., ATP level. All studied endpoints were affected by Sherpa 100 EC, and the activities of AChE and EST as well as ATP levels were affected by Dursban 480 EC. Moreover, complex interactions between all three insecticides affected ATP levels, showing outcomes that cannot be predicted when testing each insecticide separately. The results indicate that even if interactive effects are sometimes difficult to interpret, there is a need to study such interactions if laboratory-generated toxicity data are to be extrapolated to field conditions.

Microbe and bioprocess performances for sustainable production of biobased 2,3-butanediol in a sugarcane biorefinery; a technoeconomic and environmental analysis

AbstractIndustrial production of bio-based 2,3-butanediol via microbial conversion of sugars is intended to provide viable investment opportunities accompanied by reduced greenhouse gas emissions, compared to current fossil-based products. The potential impacts on the product minimum selling price and life cycle greenhouse gas emissions of further technology developments resulting in enhanced product yield, volumetric productivity and/or titres were assessed though a 33 full-factorial design. Aspen Plus® was employed to simulate multiple scenarios for 2,3-butanediol production from A-molasses in a biorefinery annexed to an existing sugarcane mill for subsequent techno-economic analysis. A 10% singular improvement in product yield, titre and volumetric productivity reduced the minimum selling price by 3.6%, 1.4% and 0.1%, whereas titre improvements reduced greenhouse gas emissions twice as much as product yield for a 10% step change. At the current state of technology, biobased 2,3-butanediol can achieve the minimum performance required to be a feasible alternative to fossil-based 2,3-butanediol with an estimated best minimum selling price of 1434$ t−12,3-BDO and greenhouse gas emissions 6.5 times less than those recorded for fossil-derived 1,4-butanediol. The minimum selling price and greenhouse gas emissions values can be reduced further by at least 16% and 14%, respectively, warranting further investment in strain and bioprocess performance enhancement. Overall, the research demonstrated that technological efforts intended to enhance the viability of biobased 2,3-butanediol production also minimized greenhouse gas emissions, integrating environmental and economic objectives for a sustainable bioeconomy. Graphical abstract

The Effects of Incorporating Nanoclay in NVCL-NIPAm Hydrogels on Swelling Behaviours and Mechanical Properties.

Following the formulation development from a previous study utilising N-vinylcaprolactam (NVCL) and N-isopropylacrylamide (NIPAm) as monomers, poly(ethylene glycol) dimethacrylate (PEGDMA) as a chemical crosslinker, and Irgacure 2959 as photoinitiator, nanoclay (NC) is now incorporated into the selected formulation for enhanced mechanical performance and swelling ability. In this research, two types of NC, hydrophilic bentonite nanoclay (NCB) and surface-modified nanoclay (NCSM) of several percentages, were included in the formulation. The prepared mixtures were photopolymerised, and the fabricated gels were characterised through Fourier transform infrared spectroscopy (FTIR), cloud-point measurements, ultraviolet (UV) spectroscopy, pulsatile swelling, rheological analysis, and scanning electron microscopy (SEM). Furthermore, the effect of swelling temperature, NC types, and NC concentration on the hydrogels' swelling ratio was studied through a full-factorial design of experiment (DOE). The successful photopolymerised NC-incorporated NVCL-NIPAm hydrogels retained the same lower critical solution temperature (LCST) as previously. Rheological analysis and SEM described the improved mechanical strength and polymer orientation of gels with any NCB percentage and low NCSM percentage. Finally, the temperature displayed the most significant effect on the hydrogels' swelling ability, followed by the NC types and NC concentration. Introducing NC to hydrogels could potentially make them suitable for applications that require good mechanical performance.

Bioethanol production from dilute acid hydrolysis of cassava peels, sugar beet pulp, and green macroalgae (Ulva lactuca)

This study presents the characterization and application of different pretreatment possibilities to optimize simple carbohydrate production from representative feedstock, followed by ethanol production through optimized saccharification steps using suitable microorganisms. Total soluble carbohydrates and carbohydrate yield were evaluated using dilute acid hydrolysis of biomass and reference materials (starch and cellulose) under different conditions. To explore two-factor interactions, the Plackett-Burman design of experiments was used in the statistical screening of variables and then transformed to a full-factorial design based on the significant variables. According to the experimental findings, dilute acid hydrolysis can yield a considerable amount of soluble carbohydrates. The goodness of fit of the model was evaluated using the coefficient of determination. The optimized carbohydrate yields of the biomass were 0.76 g/g cassava peels, 0.35 g/g sugar beet pulp, and 0.36 g/g green macroalgae. Bioethanol production from optimized hydrolysis conditions using Saccharomyces cerevisiae and Scheffersomyces stipitis DSM 3651, led to a maximum ethanol yield of 0.286 g/g from cassava peels and 0.033 g/g from sugar beet pulp, while using Neurospora intermedia DSM 1265 led to a maximum ethanol yield of 0.076 g/g from green macroalgae. Hence, these biomasses are considered valuable feedstock for bioethanol production with cassava peels having the highest potential.

Factorial design-aided study of different fluorogenic approaches for determination of the preservative natamycin in various matrices

Natamycin (NATA) is a widely used food preservative. It is a non-fluorescent compound; hence, a derivatization reaction to produce a fluorescent derivative was necessary for its analysis. In this context, two derivatization reactions were applied to develop two spectrofluorimetric methods for determining NATA. Method I relies on Hantzsch condensation reaction between the primary amine group of NATA and acetylacetone-formaldehyde (AAF) reagent to produce a highly fluorescent dihydropyridine derivative that is measured at λem/λex of 481/415 nm, while Method II based on the reaction of the primary amine group of NATA with o-phthalaldehyde in the presence of N-acetylcysteine which resulted in isoindole fluorophore derivative that can be monitored at λem/λex of 433/338 nm. The experimental variables of both methods were optimized using full-factorial designs. Under the optimum conditions, the proposed methods enabled the determination of NATA over concentration ranges of 50.0–500.0 ng mL−1 (LOD = 13 ng mL−1) for Method I and 0.50–10.0 μg mL−1 (LOD = 0.16 μg mL−1) for Method II. Both methods were efficiently applied to assay NATA in ophthalmic eye drops. The application was extended to include bread and environmental water samples based on the nano-scale sensitivity of Method I.

Development, optimization and characterization of cisplatin loaded cubosomes for human lung carcinoma

Objectives This study aimed to develop, optimize and evaluate glyceryl monooleate (GMO) based cubosomes as a drug delivery system containing cisplatin for treatment of human lung carcinoma. Significance The significance of this research was to successfully incorporate slightly water soluble and potent anticancer drug (cisplatin) into cubosomes, which provide slow and sustained release of drug for longer period of time. Methods The delivery system was developed through top-down approach by melting GMO and poloxamer 407 (P407) at 70 °C and then drop-wise addition of warm deionized water (70 °C) containing cisplatin. The formulation then exposed to probe sonicator for about 2 min. A randomized regular two level full factorial design with help of Design Expert was used for optimization of blank cubosomal formulations. Cisplatin loaded cubosomes were then subjected to physico-chemical characterization. Results The characterization of the formulation revealed that it had a sufficient surface charge of −9.56 ± 1.33 mV, 168.25 ± 5.73 nm particle size, and 60.64 ± 0.11% encapsulation efficiency. The in vitro release of cisplatin from the cubosomes at pH 7.4 was observed to be sustained, with 94.5% of the drug being released in 30 h. In contrast, 99% of cisplatin was released from the drug solution in just 1.5 h. In vitro cytotoxicity assay was conducted on the human lung carcinoma NCI-H226 cell line, the cytotoxicity of cisplatin-loaded cubosomes was relative to that of pure cisplatin solution, while blank (without cisplatin) cubosomes were nontoxic. Conclusions The obtained results demonstrated the successful development of cubosomes for sustained delivery of cisplatin.

Application of 32 factorial design for loratadine-loaded nanosponge in topical gel formulation: comprehensive in-vitro and ex vivo evaluations.

Loratadine (LoR) is a highly lipophilic and practically insoluble in water, hence having a low oral bioavailability. As it is formulated as topical gel, it competitively binds with the receptors, thus reducing the side-effects. The objective of this study was to prepare LoR loaded nanosponge (LoR-NS) in gel for topical delivery. Nine different formulations of emulsion were prepared by solvent evaporation method with polyvinyl alcohol (PVA), ethyl cellulose (EC), and dichloromethane (DCM). Based on 32 Full Factorial Design (FFD), optimization was carried out by varying the concentration of LOR:EC ratio and stirring rate. The preparations were subjected for the evaluation of particle size (PS), in vitro release, zeta potential (ZP) and entrapment efficiency (EE). The results revealed that the NS dispersion was nanosized with sustained release profiles and significant PS. The optimised formulation was formulated and incorporated into carbopol 934P hydrogel. The formulation was then examined to surface morphological characterizations using scanning electron microscopy (SEM) which depicted spherical NS. Stability studies, undertaken for 2months at 40 ± 2°C/75 ± 5% RH, concluded to the stability of the formulation. The formulation did not cause skin irritation. Therefore, the prepared NS hydrogel proved to be a promising applicant for LoR as a novel drug delivery system (NDDS) for safe, sustained and controlled topical application.

The effects of protein supplementation, fumagillin treatment, and colony management on the productivity and long-term survival of honey bee (Apis mellifera) colonies.

In this study, we intensively measured the longitudinal productivity and survival of 362 commercially managed honey bee colonies in Canada, over a two-year period. A full factorial experimental design was used, whereby two treatments were repeated across apiaries situated in three distinct geographic regions: Northern Alberta, Southern Alberta and Prince Edward Island, each having unique bee management strategies. In the protein supplemented treatment, colonies were continuously provided a commercial protein supplement containing 25% w/w pollen, in addition to any feed normally provided by beekeepers in that region. In the fumagillin treatment, colonies were treated with the label dose of Fumagilin-B® each year during the fall. Neither treatment provided consistent benefits across all sites and dates. Fumagillin was associated with a large increase in honey production only at the Northern Alberta site, while protein supplementation produced an early season increase in brood production only at the Southern Alberta site. The protein supplement provided no long-lasting benefit at any site and was also associated with an increased risk of death and decreased colony size later in the study. Differences in colony survival and productivity among regions, and among colonies within beekeeping operations, were far larger than the effects of either treatment, suggesting that returns from extra feed supplements and fumagillin were highly contextually dependent. We conclude that use of fumagillin is safe and sometimes beneficial, but that beekeepers should only consider excess protein supplementation when natural forage is limiting.

Adipokines in depressed women with and without adverse childhood experiences

BackgroundAdverse childhood experiences (ACE) elevate the risk of both major depressive disorder (MDD) and metabolic diseases. The underlying pathophysiology might include alterations of adipokine levels as a consequence of ACE. In this study, we used a full-factorial design to investigate the levels of select adipokines in women with ACE-only (n = 23), MDD-only (n = 27), ACE+MDD (n = 25) and healthy controls (HC, n = 29) to identify metabolic makers associated with vulnerability and resilience of developing MDD after ACE exposure. MethodsSerum levels of adiponectin, leptin, adiponectin-to-leptin (A/L) ratio, and retinol binding protein 4 (RBP4) were measured using enzyme-linked immunosorbent assay (ELISA). ResultsAdiponectin levels did not differ between groups. Individuals with vs. without MDD showed higher leptin serum concentrations. As predicted, A/L ratio indicated lower values in individuals with vs. without ACE. RBP4 showed a more nuanced pattern with reduced levels in the ACE-only and MDD-only groups compared to HC. Furthermore, the ACE-only group showed lower RBP4 concentrations compared to ACE+MDD. These results were not accounted by BMI or medication status. ConclusionOur results do not support the utility of adiponectin and leptin as predictors of vulnerability or resilience of developing MDD after ACE. In contrast, RBP4 might play a role in resilience towards the development of MDD following ACE. Further research on this more recently discovered adipokine seems warranted.

Biogenic engineered zinc oxide nanoparticle for sulfur black dye removal from contaminated wastewater: comparative optimization, simulation modeling, and isotherms

ABSTRACT This research work aimed to isolate and culture the bacterium Bacillus paramycoides for biogenic fabrication of zinc oxide nanoparticles, specifically ZnO and ZnO-ME nanoparticles (nanoparticles fabricated from bacterial extracts only – ZnO, and from bacterial cell mass including extract – ZnO-ME). SEM investigation revealed the spherical-shaped NPs with 22.33 and 39 nm in size for ZnO and ZnO-ME, respectively. The Brunauer, Emmett, and Teller (BET) studies revealed mesoporous structure with pore diameters of 13.839 and 13.88 nm and surface area of 7.617 and 33.635 m2/gm for ZnO and ZnO-ME, respectively. Various parameters for the adsorption of sulfur black dye onto both ZnO and ZnO-ME were screened and optimized using Plackett–Burman Design (PBD), Full Factorial Design (FFD) and Central Composite Design (CCD). The results of the optimization modeling study revealed that FFD yielded the most predictable and best-fitting results among all the models studied, with R 2 values of 0.998 for ZnO and 0.993 for ZnO-ME. Notably, ZnO-ME exhibited a greater dye removal efficiency 80% than ZnO i.e., 71%, it may be due to the presence of amorphous carbon on the surface of ZnO-ME. Among the various isothermal models, the Freundlich model displayed the strongest correlation with the dye removal data, confirming the multilayer adsorption of dye on both nanoparticles and supporting physisorption. Therefore, ZnO and ZnO-ME nanoparticles have been proven as potential tools for mitigating environmental impacts associated with dye-containing wastewater.

DEVELOPMENT AND EVALUATION OF NANOBIOCOMPOSITE TOPICAL FORMULATION

Objective: The proposed research involving transferosomes within a hydrogel matrix offers a promising approach for enhanced wound healing. This system aims to facilitate the dermal delivery of nanosized curcumin while incorporating Ascorbic acid and Salicylic acid. The integration of these components holds the potential for advancing chronic wound therapy. Methods: Curcumin transferosomes were formulated by the lipid thin film hydration method and further optimization was carried out using 32 full factorial design. The transferosome formulation, prepared using phospholipon 90G, involved selecting specific variables: the quantity of edge activator and sonication duration as independent factors, while the optimization process considered particle size and entrapment efficiency as dependent variables. Following the optimization of the transferosomes, a hydrogel formulation was developed using the central composite design approach. Results: Optimized transferosome (Batch F8) showed 87.75±3.74 nm (nanometer) particle size and 91.18±2.71% entrapment efficiency. Hydrogel was formulated by Central composite design, selecting pH and spreadability as dependent factors, to which was added curcumin transferosomes, Ascorbic acid and Salicylic acid. The data was analyzed using Stat-ease Design-Expert v7.0.0 software. The optimized batch F3 showed a pH of 6.84, spreadability of 12.89 gm. cm/sec and Curcumin release of 87.47%. Drug release from nanobiocomposite hydrogel was evaluated using the in vitro study of the formulation. The various kinetic models were applied to in vitro release data for the prediction of the drug release kinetic mechanism. The release constants were calculated from the slope of appropriate plots, and the regression coefficient (R2) was determined. It was found that the in vitro drug release of the formulation was best explained by Higuchi as the plots show the highest linearity. The regression coefficient (R2) was found to be 0.907, 0.9266 and 0.9536 for Ascorbic acid, Salicylic acid and Curcumin, respectively. Conclusion: The nanobiocomposite topical formulation was thus prepared, tested and for skin irritancy study. There is no noticeable signs of erythema, edema, or inflammation were observed on the skin. These results indicate that the developed transdermal formulation does not cause skin irritation and can be considered non-irritating.