Fruit Skin Research Articles

Bioplastic Prototype of Gayam Fruit and King Banana Skin with Glycerol Variation

Plastic is a popular packaging material used worldwide. Plastic has advantages such as being strong, light and elastic, but due to its slow decomposition process, its existence has an impact on the accumulation of plastic waste and the environment. Bioplastics made from Gayam starch and plantain peels with variations of glycerol are the best solutions to overcome the plastic problem because they are made from easily decomposed raw materials. The purpose of this study was to determine the bioplastic prototype of gayam fruit and plantain peel with glycerol variations. This type of research is quantitative with a laboratory experiment approach. This research was conducted on 24 February 2022 to 06 January 2023 at the Ambon IAIN MIPA Laboratory. Gayam fruit starch and plantain peel starch flour were made in a ratio of 1: 3. Then precipitated for 24 hours and dried in an oven for 4 hours at 50°C. Bioplastics are made by adding variations of glycerol 5%, 10%, 15%, and 20%. Bioplastic prototypes in the form of physical appearance, biodegradable tests and water resistance were analyzed descriptively using the ANOVA test (Analysis of Variance) and continued with Duncan's test to determine the significance of glycerol variations on bioplastic prototypes. The results showed that Gayam starch and plantain peel with variations in glycerol had a significant effect (P<0.05) on the bioplastic prototype.

Spreadability, Total Dissolved Solids and Likeability of Mango Jam with a Combination of Mango Flesh and Peel Arum Manis (Mangifera Indicates L)

After being harvested, mango fruit will quickly spoil due to the high water content in the fruit, so efforts need to be made to process it to extend its shelf life, one of which is processing it into jam. To get good jam you need the right composition of sugar, acid and pectin. The part of the mango fruit that is widely consumed and processed is the flesh, while the skin and seeds are not widely used even though the skin of the mango fruit contains quite high pectin. This research aims to determine the effect of the combination of mango flesh and mango skin on spreadability, total soluble solids, and panelists' preferences for jam. The method used is Completely Randomized Design (CRD) 1 factor, namely a combination of mango skin and mango flesh with 5 treatment levels: P1 (20%: 80%), P2 (30%: 70%), P3 (40%: 60%), P4(50%: 50%) and P5 (60%: 40%). Parameters observed: spreadability, total dissolved solids, and preference test. Data were analyzed using Variance Analysis and the smallest significant difference (if there is a difference) with a confidence level of 5%. The liking test will be carried out on 30 panelists (untrained) using a 5-level liking scale: very dislike (1), dislike (2), neutral (3), like (4) and very like (5). The results of the study showed that the higher the use of mango peel pulp and the less mango fruit pulp, the lower the spreading power, where the highest spreading power was in treatment P1 and was not different in treatment P2. However, the total dissolved solids value is getting higher. Panelists liked the color, taste and spreadability of treatments P1, P2 and P3 (not significantly different), while the aroma was preferred up to treatment P4.

PENENTUAN KADAR KAFEIN DALAM BUNGA, BIJI, KULIT BUAH DAN DAUN KOPI ARABIKA (Coffea arabica) WONOLELO MENGGUNAKAN SPEKTROFOTOMETER UV

Arabica coffee is a type of coffee that is widely cultivated in Indonesia. Caffeine is one of the secondary metabolites contained in coffee with various biological benefits. Caffeine belongs to the class of alkaloids of the methylxanthine group. Caffeine is distributed in different parts of the coffee plant in the leaves, cotyledons and flowers. Various studies have proven that the caffeine content in each part of the coffee plant has varying levels. This study aims to determine which part of the Arabica coffee plant comes from Wonolelo, Temanggung with the highest caffeine content.
 Powder of flowers, leaves, seeds and fruit skins of Arabica coffee were extracted with distilled water and then extracted liquid-liquid with chloroform, the chloroform fraction obtained was evaporated to form crystals containing caffeine. The results were analyzed quantitatively by UV spectrophotometry. The absorbance results were obtained from UV spectrophotometric readings, then the caffeine content in the sample was calculated and analyzed using SPSS software.
 Quantitative test stated that the highest to the lowest caffeine content were fruit peels of 3.232%, seeds (0.771%), flowers (0.509%), and leaves (0.24%). The results of this study are different compared to previous studies. This can occur due to differences in extraction methods, sample age, roasting conditions, grinding degree, geographical origin, such as growing locations at different altitudes, soil types, rainfall, harvest time, and intensity of exposure to sunlight.

Morphological, biochemical and health promoting properties in seed propagated quince fruits found in Çoruh Valley in Türkiye

Pome fruits including apples and pears widely recognized species and shows rich morphological and biochemical properties. However, the studies on the other pome fruits including quince are scarce in literature. Quince is one of the most diverse specie in the pome fruits and, in particular, its fruits are rich in bioactive compounds. Türkiye, China and Uzbekistan are leading country for world quince production. Due to self-pollination characteristics, it is possible to obtain high quality quince genotypes from seeds. In this study, some important fruit properties of ten seed propagated quince genotypes naturally found in Aras valley, located in the eastern Anatolia region of Türkiye were investigated. The genotypes differed each other for most of the morphological, biochemical and human health promoting properties. Fruit weight were in range of 205-389 g among genotypes. Seven genotypes were found pear-shaped (pyriformis) and the rest of the genotypes were apple-shaped (maliformis). Fruit firmness ranged from 5.12 kg/cm2 to 8.30 kg/cm2, respectively. Fruit skin Chroma and Hue values were found between 47.34-65.67 and 71.98-89.17, respectively. SSC (Soluble Solid Content), Vitamin C and total phenolic content of the genotypes ranged from 9.7-13.4%, 4.2-11.2 mg per 100 g FW (fresh weight), 290–432 mg gallic acid equivalent per 100 g FW, respectively. This work constitutes an important step in the conservation of quince genetic resources in the eastern Anatolia.

Determination of fruit characteristics of some fig genotypes (Ficus carica L.) obtained by selection breeding in the eastern Mediterranean region

Fig (Ficus carica L.) is a fruit species whose cultural history is old, what is making it related to the Ficus genus of the Moraceae (Mulberry) family. The study was conducted in Kahramanmaras and Osmaniye, that are located in the Eastern Mediterranean Region, in 2016-2022. Fig cultivation is extensive in that area and at the same time in the natural fig plantation regions. Pomological and phenological analyzes were carried out on 20 fig genotypes determined in the research. The fruit weight of the fig genotypes varied between 80.79 - 40.37 g, and the average peel thickness was between 3.79-2.28 mm. The highest soluble solids value is in the genotypes 46-OS-1 at 25%, whereas the lowest soluble solids value is in the genotypes 46-TR-9 and 80-DZ-2 at 17%, and the highest acidity value is 46-OS-3 at 0.37%. The Figs genotypes were divided into two main groups in terms of fruit shape: flattened spherical and round. All genotypes were either short or moderately necked apart from one genotype. The majority of genotypes were found in yellow tones, some in purple tones, and the color of the fruit flesh was mostly in amber tones in terms of the color of the fruit skin. As a result of the study, it was revealed that Kahramanmaras and Osmaniye provinces in the Eastern Mediterranean Region have fig natural distribution areas and new varieties can be developed by selection breeding in these localities.

Molecular evolution and expression assessment of DFRs in apple

BackgroundAnthocyanins are the secondary metabolites of flavonoids in plants. As a key enzyme in the biosynthetic pathway of anthocyanin, dihydroflavonol 4-reductase (DFR) act as an important regulatory point, but DFR family genes has not been systematically characterized in apple (Malus domestica Borkh.).MethodsThe members of DFR genes in apple were identified and their gene structure, chromosome distribution, evolutionary relationships, collinearity, cis-component and protein interaction relationships were predicted using bioinformatics methods. The expression patterns of MdDFRs in various organs, such as leaves, fruit flushes, fruits, ripe fruit peels, flowers and stems were analyzed using GeneChip expression array analysis. qRT-PCR was employed to analyze the expression levels of MdDFRs in different apple varieties with varying levels of fruit skin at maturity.ResultsThe apple database revealed 96 DFR genes, which are distributed on 17 chromosomes and can be divided into 3 subfamilies. These 96 DFR genes were mostly composed of α-helix and random coil according to secondary structure prediction, and were mainly expressed in chloroplasts and cytoplasm. MYB binding site involved in flavonoid biosynthetic genes regulation element (MBSI) was identified in the promoter of MdDFR15/76/81/89/90/91/93/94. Lignin/flavonoid synthesis-related elements of MYB recognition site and MYB-binding site were identified in the promoters of MdDFR05/09/13/19/22/24/26/30/31/33/34/46/50/52/54/64/65/69/75/76/79/86. The internal collinearity analysis of the apple MdDFR genome revealed a total of 34 pairs of duplicated gene pairs. Interspecific collinearity analysis showed that there were 66 and 57 homologous gene pairs in apple/tomato and apple/grape, respectively. GeneChip expression array analysis showed that MdDFR72 and MdDFR96 were higher expressed in ripe fruit fleshes and peel, MdDFR01/06/67/49/54/91 were higher expressed in flowers, MdDFR64 was higher expressed in ripe fruit peels and flowers than those of other tissues. Besides, 75 MdDFR proteins interacted directly or indirectly with anthocyanidin synthesis related proteins MdANS, MdF3H, MdMYB1, MdMYBPA1 to form a protein interaction network. Interestingly, MdDFR69 and MdDFR87 had direct interactions with these four proteins, MdDFR64 had direct interactions with MdANS and MdF3H. qRT-PCR analysis showed that the expression levels of MdDFR01/05/31/53/64/69/73/84/87/94/96 were up-regulated with the accumulation of anthocyanins.ConclusionsThis study lays a foundation for further research on the function of DFR genes in apple.Graphical

Sensory Evaluation, Biochemical, Bioactive and Antioxidant Properties in Fruits of Wild Blackthorn (Prunus spinosa L.) Genotypes from Northeastern Türkiye

Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small farmers constituting a real safety valve for the sustainability of the processing plum value chain. Fruits of those plum genotypes differ in their biometric, processing and functional quality attributes. In this study, fruits of eight wild grown blackthorn (Prunus spinosa) genotypes were sampled from the Ispir district of the Erzurum province and subjected to sensory, morphological, biochemical and antioxidant characterization. Aroma, taste and juiciness were used as the criteria for sensory analysis, and a trained panel of ten experts established and evaluated the sensory characteristics of the fruits of the blackthorn. Fruit weight, fruit skin and flesh color as L*, a* and b* values were the main morphological parameters. For biochemical and bioactive analysis, organic acids, SSC (Soluble Solid Content), vitamin C, total anthocyanins, total phenolic content and total antioxidant capacity were determined. Antioxidant capacity was determined by FRAP (ferric reducing antioxidant power) assay. The results indicated significant differences among genotypes for most of the traits. The fruit weight was found between 2.78–3.67 g. The skin L*, a* and b* values were 13.11–16.12, 2.56–3.85 and 2.01–3.44, respectively. The flesh L*, a* and b* values were in the ranges of 17.45–20.37, 4.88–6.73 and 4.12–5.66, respectively. The SSC content ranged from 18.66% to 21.07%. The total phenolic content (TPC), total anthocyanin content (TAC) and ferric reducing antioxidant power (FRAP) were between 372–504 mg GAE/100 g; 53–72 mg cy-3 g eq./100 g and 107–134 mmol Fe (II) eq./g, respectively. The dominant organic acid was malic acid for all genotypes and varied from 1.04 g/100 g to 1.52 g/100 g fresh weight base. The data showed that the analyzed blackthorns, particularly PS-5, PS-3 and PS-2 had bigger fruits indicate their suitability for fresh and dried consumption, PS-1 and PS-3 had higher juiciness, indicating their suitability for processing, and PS-4 and PS-6 had higher human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants.

Watering Volume and Growing Design’s Effect on the Productivity and Quality of Cherry Tomato (Solanum lycopersicum cerasiformae) Cultivar Ruby

It is intriguing to understand the influence of the watering volume and growing design on the growth, yield, and quality of cherry tomatoes. This study sought to identify the most effective watering volume and growing design treatment for the production and quality of the cherry tomato cultivar Ruby. This study was an exploration using a factorial experiment with an RCBD design. The treatment factors included the growing design (greenhouse, rain shelter, and screen house) and the watering volume (100% ETc, 75% ETc, and 50% ETc). The results showed that the root dry weight and root–shoot ratio were higher in the screen house design, while the fruit firmness was higher in the rain shelter design. Considering the effect of the watering volume, a higher fruit diameter, heavier fruits, more fruits per plant, higher ultimate fruit and biological yield per plant, higher root and shoot biomass, better root–shoot ratio, higher leaf chlorophyll content, greater fruit skin firmness, and greater elasticity were noted for the 100% ETc treatment. In contrast, the growth rate was higher for the 50% ETc treatment. Cultivating cherry tomatoes in a greenhouse using a watering volume of 100% ETc is recommended based on the results. These conditions led to better growth, higher fruit yield, and improved fruit quality, making them favorable options for successful cherry tomato production.

Decrease in the number of Streptococcus mutans and Staphylococcus aureus bacterial colonies after administration of sentul fruit peel extract gel (Sandoricum koetjape) in gingivitis model of white Wistar rats

Link of Video Abstract: https://youtu.be/NpcREsYIlEE Introduction: Sentul fruit peel extract (Sandoricum koetjape) has the ability to bacterium Streptococcus mutans and Staphylococcus aureus after administration of Sentul fruit peel extract gel on gingivitis model white Wistar rats. Utilization of extracts from the skin of sentul fruit (Sandoricum koetjape) as well as the proper use of natural sources will have an impact on the prevention and healing of gingivitis. The study aimed to determine the decrease in the number of Streptococcus mutans and Staphylococcus aureus bacteria colonies after administration of nano gel of sentul fruit peel extract in the gingivitis model of white Wistar rats. Method: study design with experimental animal models. The data obtained by the One-Way ANOVA test and if the effect is significant continue with Duncan's test at the 5% level. Result: There was a decrease in the number of Streptococcus mutans and Staphylococcus Aureus bacteria colonies after administration of Sentul fruit peel extract gel in the gingivitis model of white Wistar Rats. The best gel concentration was Sentul peel extract gel 2.4% with the smallest number of bacterial colonies, Staphylococcus aureus colonies of 2.2 x 102 ± 1.9 x 101 colony forming unit (cfu) and the number of Streptococcus mutans colonies of 2.6 x 102 ± 1.2 x 101 CFU. Conclusion: Sentul fruit peel extract 2.4% effectively inhibits the growth of Streptococcus mutans and Staphylococcus Aureus in white Wistar rat models with gingivitis.

Chilling injury control in pomegranate fruit with compostable stretchable skin film

The use of stretch films as a second skin over fruits is a common technological solution to prevent their deterioration during postharvest storage. However, these films pose an environmental problem, and the management of this waste is a challenge. To address this issue, a new biodegradable polymer composed of a blend of butylene adipate-co-terephthalate and polylactic acid (PBAT-PLA) called Nature Fresh® has been developed to produce stretch films suitable for post-harvest use without environmental concerns. Pomegranate is a fruit that is highly sensitive to Chilling Injury (CI) at temperatures below 5 °C. To evaluate the suitability of Nature Fresh® film for pomegranate packaging, two cultivars with different sensitivity to CI, 'Bigful' and 'Mollar de Elche', were wrapped with the film and stored for 56 days at 2 °C plus 2 days at 20 °C. Control groups included fruits without film and fruits wrapped with stretch film made of linear low-density polyethylene (LLDPE), which is commonly used in post-harvest storage. Pomegranates packed in PBAT-PLA and LLDPE films showed similar evolution in the appearance of internal and external husk CI, respiration rate, ion leakage, total polyphenol, and anthocyanin content, these parameters being lower than in control fruit. However, due to differences in water vapor and gas permeability between the two films, weight loss and firmness reduction were significantly higher in pomegranates wrapped in PBAT-PLA compared to LLDPE, but this did not impact any detrimental effect on overall appearance. Internal CI was significantly (P < 0.001) positively correlated with fruit respiration rate, ion leakage, weight loss, total polyphenol, and anthocyanin content; while it was negatively correlated with fruit firmness, skin and aril color, soluble solids, acidity, and maturity index. In summary, the use of PBAT-PLA film presents a feasible substitution to LLDPE on maintaining the quality of pomegranates during post-harvest storage, while also aligning with worldwide initiatives aimed at decreasing plastic waste.

Metode ohmic heating untuk ekstraksi betasianin sebagai pewarna pangan dari kulit buah naga merah (Hylocereus polyrhizus)

Natural food colorant is increasingly being developed and applied to food products. Natural food coloring is safer to use because it does not cause side effects to health. Indonesia is rich in the availability of red dragon fruit. In general, people only eat dragon fruit flesh, while the skin is not used. Red dragon fruit skin is rich in betacyanin pigment which produces a red-violet color. This pigment needs to be extracted so that it is separated from other skin components. Extraction using ohmic heating technology can be a solution to extract betacyanin pigment. The aim of the study was to determine the characteristics of the betacyanin pigment from red dragon fruit skin extracted using the ohmic heating method. The study used a factorial randomized block design consisting of two factors, the first factor was the type of solvent (aquades, 0.25% NaCl solution, 0.25% citric acid) and the second factor was the amount of voltage (30 V, 40 V, 50 V). The results showed that the highest betacyanin content was found in the 0.25% NaCl solvent treatment, which was 5.15 mg/L. Extracts stored in the refrigerator for 5 days did not show a significant change in extract betacyanin pigment levels.

Utilization of dragon fruit (Hylocereus polyrhizus) peels waste as a natural dye

Dragon fruit has many functions and benefits for the health of both the fruit and the skin. One of the waste products of dragon fruit that has not been fully utilized is the skin of the red dragon fruit. The anthocyanin pigment that gives color to the red dragon fruit is found in the peel. Maltodextrin encapsulation is one of the methods used to preserve anthocyanin pigments. Therefore, the purpose of this study is to make anthocyanin powder from the skin of the red dragon fruit using the encapsulation method as a food coloring and to ensure an ideal concentration of maltodextrin for the physical and chemical properties of the powder. The experimental design used a one factor Completely Randomized Design (CRD) with the addition of 10%, 20%, 30%, and 40% maltodextrin concentrations. Analysis of the product involves measurements of color saturation, yield, water content, and total anthocyanin. The outcomes demonstrated that the chosen powder from the four treatments was obtained with the addition of maltodextrin at a concentration of 30% with a color intensity of 58.45 L*, color a* of 11.77, color b* of 15.53, yield of 50.344%, water content of 7.847% and total anthocyanin of 148.240 ppm.

Pectin/agarose-based coating functionalized with Piper betel L. extract for enhancing storability of postharvest bananas

The goal of this study is to produce an active coating on Chiquita bananas storage at 20 °C and 64 %RH using a combination of pectin and agarose (PeA) supplemented with Piper betel L. leaf extract (PBE) to extend their shelf life. The introduction of 30 wt% PBE into the PeA film improved the tensile strength, flexibility, UV resistance as well as antioxidant and antibacterial properties of resulting films. The coating solutions, containing PeA functionalized with PBE, were applied for prolonging the shelf life of postharvest bananas using the dipping method. Scanning electron microscope (SEM) analyses showed that PeA coating solution enriched with 30 wt% PBE (PeA-PBE-30) coating effectively sealed and uniformly dispersed on the fruit skin. Besides, PeA-PBE-30 coating can significantly reduce the respiration rate of fresh bananas during 8 d storage period. Other factors including weight loss, total soluble solids, and pH were also reduced, while the retention of titratable acid also indicated the protective efficacy of PeA-PBE-30 coating compared to uncoated fruits and other treatments. This study provides evidence that pectin/agarose-based coating enriched with PBE can enhance the storability of fresh bananas, making it a promising strategy for commercial use.

Registration certificate for local variety of curly red chili Pengkol Jati from Grobogan, Central Java, Indonesia

Pengkol Jati curly red chili is a local variety of chili from Grobogan Regency, Central Java that has been grown by farmers for decades. A certificate of ownership of local varieties issued by Center for Crop Variety Protection and Agricultural Licensing, Ministry of Agriculture of Republic Indonesia is very important to protect the variety from illegal name and to meet the requirements for the release of varieties. The primary requirement to get the certificate of ownership is morphological identification, which was conducted in February to April 2020 in Pengkol Jati village, Grobogan regency. The character observed include external appearance of tree (height, growth, head shape, crown, branching, color on the book, canopy, stem shape and color), leaves (shape, size, direction, leaf color, size and color petiole, wax layer), flower (color of flower; stem; and flower crown, stain, petals, pistil head,position and angle of flower stalk, number of flower per node, flowering schedule, time of flower to fruit,), fruit (color, size, shape, skin and flesh thickness, fruit weight, seed color, shape, and size), and general data. The morphological characteristics and general data were noted and organized in the form of a standard blank and registered to Center for Crop Variety Protection and Agricultural Licensing to get a Certificate of Ownership of Local Varieties. Result of the experiment revealed that in 18 December 2020, a Certificate of Ownership of Local Varieties of Pengkol Jati curly red chili numbered: 1609/PVL/2020 was issued and submitted to local government of Grobogan regency.

New Germplasm for Breeding: Pink-flowered and White-fruited Strawberry

Most strawberry plants have white flowers and red fruit. We developed a new strawberry selection with pink flowers and white fruit, and named it G23. Basic phenotypic data were recorded over years of observation and experimentation with the flower crown diameter, petal color, and rate of fruit set, as well as fruit skin color, flesh color, seed color and attachment status, fruit weight and shape, soluble solids contents, and firmness. We found that G23 bloomed with a stable pink flower and produced white fruit consistently with a relatively high fruit-set rate compared with its female parent, ‘Pink Panda’. G23 displayed high resistance to Fusarium wilt (Fusarium oxysporum) and anthracnose (Colletotrichum spp.). It is also tolerant of high temperatures (up to 40 °C) and long-term drought. The asexual propagation ability of G23 is high, with ∼60 to 100 stolon ramets formed during the summer. In summary, this new pink-flowered and white-fruited strawberry germplasm is suitable for ornamental use, as a result of its remarkable flowering and fruiting characteristics. In addition, it provides opportunities for innovative strawberry germplasm for future breeding.

Application of living mulch in rows of the apple trees on several rootstocks – long-term evaluation

The long-term influence of several living mulches on apple tree growth, nutrient status, yield, and fruit quality concerning the year of cover crop sowing in tree rows was evaluated in the Lower Silesia region in the southwestern part of Poland. The estimation was conducted in six apple tree ‘Ligol’ populations differentiated by the rootstock: M.26, M.9, P 60, P 2, P 16, and P 22. In experiment no. 1, one-year-old nursery stock was planted, and perennial living mulches were sown: colonial bent grass (Agrostis vulgaris With.) and white clover (Trifolium repens L.) in the same year and blue fescue (Festuca ovina L.) in the following year. In experiment no. 2, which involved two-year-old stock, the sowing of blue fescue was delayed until the second, third, fourth, and fifth years following orchard planting. The presence of the cover crop significantly affected young tree growth when one-year-old trees were planted. The sowing of the living mulch in the first or the second year after tree planting led to a significant reduction in the cumulative yield obtained from the young trees. The living mulch improved the red coloration of the fruit skin in the young orchard, but it caused a reduction in the mean fruit weight and size. However, it also contributed to decreased nitrogen concentration in the leaves of the apple trees, and the tree tolerance to living mulch increased as the orchard aged. A choice of semidwarf rootstock, postponed sowing of cover crop, and high-quality nursery stock were recognized as the most important factors for fostering apple tree tolerance to living mulches in tree rows.

Identification of R2R3-MYB family in blueberry and its potential involvement of anthocyanin biosynthesis in fruits

BackgroundBlueberries (Vaccinium corymbosum) are regarded as “superfoods” attributed to large amounts of anthocyanins, a group of flavonoid metabolites, which provide pigmentation in plant and beneficial effects for human health. MYB transcription factor is one of vital components in the regulation of plant secondary metabolism, which occupies a dominant position in the regulatory network of anthocyanin biosynthesis. However, the role of MYB family in blueberry responding to anthocyanin biosynthesis remains elusive.ResultsIn this study, we conducted a comprehensive analysis of VcMYBs in blueberry based on the genome data, including phylogenetic relationship, conserved motifs, identification of differentially expressed MYB genes during fruit development and their expression profiling, etc. A total of 437 unique MYB sequences with two SANT domains were identified in blueberry, which were divided into 3 phylogenetic trees. Noticeably, there are many trigenic and tetragenic VcMYBs pairs with more than 95% identity to each other. Meanwhile, the transcript accumulations of VcMYBs were surveyed underlying blueberry fruit development, and they showed diverse expression patterns, suggesting various functional roles in fruit ripening. More importantly, distinct transcript profiles between skin and pulp of ripe fruit were observed for several VcMYBs, such as VcMYB437, implying the potential roles in anthocyanin biosynthesis.ConclusionsTotally, 437 VcMYBs were identified and characterized. Subsequently, their transcriptional patterns were explored during fruit development and fruit tissues (skin and pulp) closely related to anthocyanin biosynthesis. These genome-wide data and findings will contribute to demonstrating the functional roles of VcMYBs and their regulatory mechanisms for anthocyanins production and accumulation in blueberry in the future study.

Realistic simulation of fruit mildew diseases: Skin discoloration, fungus growth and volume shrinkage

Time-varying effects simulation plays a critical role in computer graphics. Fruit diseases are typical time-varying phenomena. Due to the biological complexity, the existing methods fail to represent the biodiversity and biological law of symptoms. To this end, this paper proposes a biology-aware, physically-based framework that respects biological knowledge for realistic simulation of fruit mildew diseases. The simulated symptoms include skin discoloration, fungus growth, and volume shrinkage. Specifically, we take advantage of both the zero-order kinetic model and reaction–diffusion model to represent the complex fruit skin discoloration related to skin biological characteristics. To reproduce 3D mildew growth, we employ the Poisson-disk sampling technique and propose a template model instancing method. One can flexibly change hyphal template models to characterize the fungal biological diversity. To model the fruit’s biological structure, we fill the fruit mesh interior with particles in a biologically-based arrangement. Based on this structure, we propose a turgor pressure and a Lennard-Jones force-based adaptive mass–spring system to simulate the fruit shrinkage in a biological manner. Experiments verified that the proposed framework can effectively simulate mildew diseases, including gray mold, powdery mildew, and downy mildew. Our results are visually compelling and close to the ground truth. Both quantitative and qualitative experiments validated the proposed method.

SOSIALISASI INOVASI PRODUK KAYA GIZI DARI HASIL PEMANFAATAN LIMBAH BAHAN PANGAN

Food waste is still a problem in Indonesia. This is caused by the limited knowledge of the community about the benefits of food waste from a nutritional and health perspective. Food waste such as jackfruit seeds, banana peels, corn silk, avocado seeds, dragon fruit peels, tofu dregs, shells and shrimp heads also have a high enough nutritional content that they are beneficial to health. The purpose of this socialization is to provide knowledge to the public regarding the benefits of food waste in terms of nutritional aspects with the hope that it can be developed by the community. This activity was carried out during the 2023 Anniversary of Aisyah Pringsewu University by involving 35 students in the 4th semester of the Undergraduate Nutrition Study Program who were divided into 10 groups. The processing methods for food waste products are drying, roasting and frying. Products produced from food waste processing are Dragon Fruit Skin Pie, Malted Flakes (Jackfruit seed Cookies flakes, Colitis (Banana Skin Cookies), Sikju (Shrimp Skin Cheese Sticks), Dolas (Taro Flour Donuts), Crepe Mille Silk Cheese (hair flour) corn), Snackbar Upasta (Purple Sweet Potato and Tofu Dregs), Chumer (Red Bean Churros), Nastar Tekajawo (Nastar Green Bean Flour), Avocado Seeds Cookies (Avocado seed cookies).

Natural allelic variation in the EamA-like transporter, CmSN, is associated with fruit skin netting in melon.

A SNP mutation in CmSN, encoding an EamA-like transporter, is responsible for fruit skin netting in melon. In maturing melon (Cucumis melo L.), the rind becomes reticulated or netted, a unique characteristic that dramatically changes the appearance of the fruit. However, little is known about the molecular basis of fruit skin netting formation in this important cucurbit crop. Here, we conducted map-based cloning of a skin netting (CmSN) locus using segregating populations derived from the cross between the smooth-fruit line H906 and the netted-fruit line H581. The results showed that CmSN was controlled by a single dominant gene and was primarily positioned on melon chromosome 2, within a physical interval of ~ 351kb. Further fine mapping in a large F2 population narrowed this region to a 71-kb region harboring 5 genes. MELO3C010288, which encodes a protein in the EamA-like transporter family, is the best possible candidate gene for the netted phenotype. Two nonsynonymous single nucleotide polymorphisms (SNPs) were identified in the third and sixth exons of the CmSN gene and co-segregated with the skin netting (SN) phenotype among the genetic population. A genome-wide association study (GWAS) determined that CmSN is probably a domestication gene under selective pressure during the subspecies C. melo subsp. melo differentiation. The SNP in the third exon of CmSN (the leading SNP in GWAS) revealed a bi-allelic diversity in natural accessions with SN traits. Our results lay a foundation for deciphering the molecular mechanism underlying the formation of fruit skin netting in melon, as well as provide a strategy for genetic improvement of netted fruit using a marker-assisted selection approach.