Fruit Matrices Research Articles

Viral diagnosis and effectiveness of shoot tip culture for virus eradication in passion fruit (Passiflora edulis Sims) matrices used for hybrid seed production

Viral diagnosis and effectiveness of shoot tip culture for virus eradication in passion fruit (Passiflora edulis Sims) matrices used for hybrid seed production

Can cryogenic freezing preserve the quality of fruit matrices during long-term storage compared to the mechanical method?

This study compares cryogenic freezing (–80 °C and 0.5 m/s air velocity) and mechanical freezing (–40 °C and 4 m/s air velocity) conditions/methods on the quality of melon and apple tissues during a 90-day storage. Samples were analysed on the 7th, 30th, 60th, and 90th day for various qualities: pH, TSS, titratable acidity, total quality index, enzymology, vitamin C, firmness, colour, exudate, soluble protein, and microstructure. Results show pH, °Brix, titratable acidity, and total quality index changes after freezing. Trends differed between melon and apple. Melon pH increased after freezing, while apple pH decreased. Titratable acidity decreased post-freezing for both. °Brix in melon initially decreased but increased in apples with longer storage. Freezing conditions and storage durations affected total quality index. Melon firmness increased, while apple firmness decreased compared to fresh samples post-freezing. Freezing condition did not affect firmness, but storage duration impacted it. Colour was influenced by both freezing treatment and methods, as well as the duration of frozen storage for melons. The L* value decreased for both matrices following freezing treatment. The a* and b* values of apples increased after post-freezing treatment compared to fresh. The a* value of fresh and cryogenically frozen melons was similar. Colour differences were more pronounced for mechanically frozen samples, increasing during storage for apples, while a fluctuating trend was observed for melons. Apple samples exhibited a reduction in polyphenol oxidase (PPO) activity post-freezing, while peroxidase activity (POD) displayed some increase in melons. PPO activity was lower for cryogenic freezing, decreasing with storage, while POD activity was storage-duration impacted. Exudation was lower in cryogenic freezing for both matrices. Soluble protein content was higher in cryogenic frozen samples, though fluctuating. Cryogenic freezing slightly reduced pore sizes in apples, preserving microstructure better, unlike mechanical freezing which damaged cellular structure.

Effect of resolution enhancement using metal ion assisted strategy based on electrospray ionization-ion mobility spectrometry: A case study of carbendazim and thiabendazole in fruits

A method for the rapid and simultaneous determination of carbendazim and thiabendazole residues by electrospray ionization-ion mobility spectrometry (ESI-IMS) combined with a metal ion-assisted technique was developed and validated in different fruit matrices. The metal ion assisted strategy was performed instead of tedious pre-separation procedures to overcome the limitation of low resolution of IMS. Four transition metal cations, Co(II), Ni(II), Cu(II), and Zn(II), were screened and their interactions with carbendazim and thiabendazole were investigated. The injection flow rate and metal ion concentration were optimized. The Cu(II) assisted approach helped to achieve well-separated peaks with a peak-to-peak resolution of 3.61. This method was then applied to detect carbendazim and thiabendazole simultaneously in apples, pears, bananas, and mangoes. The limit of detection (LOD) were 0.03 mg kg−1 and 0.13 mg kg−1 for carbendazim and thiabendazole, respectively, while spiked recoveries were 61.5–122.0% and 83.5–119.8%, respectively, with RSDs less than 13.9%. These satisfactory evaluation parameters indicated that the approach was capable of performing quantitative analysis of multi-pesticide residues. In addition, the feasibility of using metal ion assisted-ESI-IMS for the simultaneous detection also was theoretically demonstrated through molecular electrostatic potential analysis and binding energy calculation based on density functional theory (DFT). Both experimental and theoretical results revealed the effectiveness of the metal ion assisted strategy in improving the resolution of ESI-IMS.

Ferric chloride assisted QuEChERS method for separate detection of bifenazate and bifenazate-diazene in citrus fruits and its field validation.

Bifenazate is widely recognized as an effective acaricide for citrus production in various regions. Detecting both the parent compound of bifenazate and its metabolite, bifenazate-diazene, simultaneously can be challenging owing to their tendency to undergo chemical interconversion. Current methods developed for detecting bifenazate or bifenazate-diazene residues often involve lengthy incubation periods and may not effectively separate the two compounds. In this study, we developed a convenient and fast method based on a modified QuEChERS method assisted by oxidants to concurrently detect bifenazate and bifenazate-diazene. Based on preliminary analysis, it appears that ferric chloride has the ability to react with a reducing substance present in citrus, which may prevent the reduction of bifenazate-diazene. The method was validated and applied in a field trial. This work reports a novel strategy to establish a balanced 'neutral' condition to create a potential method for efficient determination of bifenazate acaricide residues in fruit matrices.

Stability of pesticide residues in citrus fruits during storage

Introduction. Storage of selected samples before transportation to the laboratory and immediately before analysis is an important component in the process of product quality control and evaluation.
 Purpose of the work was to evaluate the degradation of pesticide active substances in real samples of citrus fruit left for long periods of storage in deep freezing conditions.
 Materials and methods. High-performance liquid chromatography with triple quadrupole mass detector (HPLC-MS/MS) was used to identify and quantify pesticide active substances. According to the method of analysis MUK 4.1.3657–20, cryogenic grinding of samples was carried out in a cutter using dry ice. Sample preparation was carried out using the QuEChERS technology. To extract analytes from a homogenized sample acetonitrile was used in the presence of salts containing citrate buffer, followed by purification of the extract by SPE.
 Results. In the studied samples of citrus, the analytes showed stability. Thirty months after storage of samples in deep freezing conditions (temperature not higher than –20 °C), the identified levels of active ingredients of the pesticides imazalil, pyrimethanil and prochloraz did not change by more than 20% compared to the previously detected concentrations. Insignificant amounts of imidacloprid, thiabendazole and pyriproxyfen, as well as traces of azoxystrobin and trifloxystrobin, detected in the study of samples, were also found in samples after long-term storage. 
 Limitation. The study is limited to the study of the stability of individual active substances found in real samples of citrus fruits intended for sale to the consumer.
 Conclusion. Proper storage of samples should ensure the preservation of the levels of active substances in the samples and exclude their change due to various processes (volatilization, enzymatic and hydrolytic decomposition, etc.), which requires the performance of preliminary experimental studies to assess their stability. The work showed the stability of residual quantities of imazalil, pyrimethanil, prochloraz, imidacloprid, thiabendazole, and pyriproxyfen in citrus fruit matrices under storage conditions in a freezer at not more than –20 °C for 30 months.

Vinegar production via spontaneous fermentation of different prickly pear fruit matrices: changes in chemical composition and biological activities.

This study focused on the valorization of prickly pear (PP) fruit (Opuntia ficus-indica) into vinegar by spontaneous surface fermentation on different starting matrices (with/without the addition of sucrose and with/without PP peel in the raw material). Different parameters were monitored during the fermentation process in terms of their physicochemical and biological properties. Physicochemical and phytochemical analysis revealed significant differences depending on the starting matrix. An increase in total phenolic content (TPC) was observed for the majority of samples when transformed from PP juice into PP vinegar revealing the role of fermentation in enhancing the bioactive compounds content. Better antioxidant and antibacterial activity were detected for vinegar samples compared with the initial starting matrix. Using whole PP fruit resulted in better TPC and antioxidant activity; in contrast, sugar addition had no significant effect on any studied data. Analysis of variance, taking into account the four factors that were studied (matrix, variety, with/without peel, and with/without sugar), demonstrated that only the factor 'presence or absence of the peel' had a significant influence on the TPC values. This study demonstrated that both whole PP fruit and PP juice could be used as new raw materials for vinegar production. © 2023 Society of Chemical Industry.

Integrated Analysis of Polycyclic Aromatic Hydrocarbons and Polychlorinated Biphenyls: A Comparison of the Effectiveness of Selected Methods on Dried Fruit Matrices

Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) are groups of chemical substances commonly found in the environment. Because of large differences in the concentrations of PAHs and PCBs in the materials tested, separate analytical methods specific to each group of compounds are usually used. The aim of this study was to compare methods for the determination of PAHs and PCBs that permit the simultaneous determination of these compounds from one solvent extract. The analysis of the content of 15 PCB congeners and 16 PAHs was conducted using dried fruits. The analyses were performed with gas chromatography coupled with mass spectrometry. PAHs and PCBs were determined separately in each fruit sample using specific extraction and cleanup procedures for the respective groups of compounds. Analyses were also performed with two methods that permitted the simultaneous analysis of PAHs and PCBs in one solvent extract. The integrated methods did not provide adequate extract cleanup of interfering substances; consequently, the results of determinations of PAHs and PCBs using these methods were significantly different from the values obtained with proven determination methods for PAHs and PCBs.

Calcium-infused dehydrated pineapple

Mineral-infused dehydrated fruits could be an easy and most convenient way to healthy snacking for those who have mineral deficiencies. An attempt is made to impregnate selected minerals into fruit matrices by applying the vacuum impregnation process. Threelevel factorial experiment designs with two factors were used in this study. In order to increase stability and assure fruit preservation, impregnated pineapple samples were dehydrated to a water content below 10%. Calcium-infused dehydrated pineapple was compared with commercially available dehydrated pineapple. The sample contains 10 times and 2 times more calcium than their fresh counterpart and commercial dehydrated pineapple respectively. Yellow colour (b* value) was equal to commercial. Moisture content was 6-7%, compatible with both samples. Firmness (g) was far less by 117-point when compared to commercial. The sample had a liking score of 6 (out of 7). Four attributes had a greater liking score than commercial, except for chewiness (compatible with commercial). The panellists accept this product, and it could be a healthy snacking to enhance daily calcium intake.

Occurrence, identification, and decontamination of potential mycotoxins in fruits and fruit by‐products

AbstractThe incidence of aflatoxins, ochratoxin A, and patulin in fruits and processed fruit products has been ever more challenging and gained additional focus on ecofriendly mitigation strategies. The onset of these toxins is due to several factors involving insect attacks, agricultural practices, and climate change. Acute and chronic health hazards are clinically proven after consuming contaminated foodstuffs, even at lower concentrations of mycotoxins. Synergistic, masked, and substantial occurrence in fruit matrices increase their complexity in detection and detoxification; apparently, this article reviewed the available information on the occurrence of mycotoxins in several fruits and their products, focused on the conventional and advanced methods of identification, quantification, and decontamination techniques. Strengthening and implementing stringent international and national guidelines are required for impactful, tangible measures in the future. Nevertheless, controlling the mycotoxins in fruits will certainly be challenging for scientists. Therefore, more impactful technologies are still needed to eliminate the toxins at the threshold level of the food chain and ensure sustainable global food safety.

Metabolomic studies of anthocyanins in fruits by means of a liquid chromatography coupled to mass spectrometry workflow

Among plant pigments, anthocyanins are one of the most important. However, there are great difficulties related to applying appropriate techniques able to identify molecules with similar structures (suchlike anthocyanins) and simultaneously estimate their variety and concentration within fruit matrices. Therefore, several methods have been evaluated to achieve the best workflows for anthocyanin analysis. For the first time, this review article presents the current findings on analytical procedures for anthocyanin investigation based on the robust liquid chromatography coupled to mass spectrometry (LC-MS) workflow. Anthocyanins extraction, LC-MS analytical instrumentation, and chemometric strategies that converge to the better performance of such workflow are herein presented. By means of this metabolomics platform, it is possible to obtain the most accurate identification of phenolic compounds, such as anthocyanins. This is a crucial step since these compounds are related to the sensory qualities of food, such as color, flavor, and aroma.

Validated QuEChERS-based UHPLC-ESI-MS/MS method for the postharvest control of patulin (mycotoxin) contamination in red-pigmented fruits

The paper outlines a procedure based on ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry and QuEChERS pretreatment for the determination of patulin in selected berries and stone fruits. The mycotoxin was determined in the positive electrospray ionization mode utilizing the formation of its methanol adduct. The method was validated for 4 fruit matrices: strawberry (Rosaceae, Fragaria), raspberry (Rosaceae, Rubus), redcurrant (Rosaceae, Prunus) and sour cherry (Grossulariaceae, Ribes). The fruits were characterized by total phenolics, flavonoids, and anthocyanin contents and obtained results were within the ranges of 2.65–11.22 mg GAE/g, 1.06–4.67 mg CE/g, 0.05–1.44 mg cyd-3-glu/g, respectively. The limits of quantification ranged from 0.65 µg/kg to 3.01 µg/kg depending on the fruit type. The method was applied to 71 fruit samples collected in Poland. Analysis of moldy fruit samples has shown a high incidence of patulin contamination.

A systematic review on fruit‐based fermented foods as an approach to improve dietary diversity

Fermented foods are popular around the globe for their health-promoting components and activity. In recent years, for promoting better health and nutrition, fortification and supplementation of fermented fruits are used as functional healthy beverage, especially for those who are intolerant to lactose-based products. Moreover, industrial revolution has transformed traditional fermentation process to a controlled process suitable for industrial scale production of functional ingredients. The aim of this systematic review is to provide an in-depth perspective of non-alcoholic fermented fruit beverages with an emphasis on improving bioactive and nutritional compounds such as phenolic compounds, vitamins, and minerals, and their effort as pro/prebiotics diets. For instance, consumption of fermented fruits and fruit-based probiotic foods such as kombucha has increased, and the evidence for effects on antioxidant and bioactive compounds is presented and discussed. Researchers use cutting-edge technology and innovations to convert fermented fruits into functional beverages with bioactive metabolites and phytochemicals. Hence, for the production of any functional beverage, it is essential to optimize the microbial strains that can utilize different fruit matrices as a single or in combination. These perspectives articulate fruit-based probiotics as one of the possibilities of processing fruit for culinary and pro-health purposes. Novelty impact statement This systematic review noticeably focuses on the fruit fermentation along with various bioactive ingredients derived from fermented fruits. In addition, comprehensive description on fruits and fruit juices as a promising carrier of probiotics, fermented fruits as dietary supplements that are in the market, and related future perspectives are discussed.

Comparison of Risk Assessment Schemes in GHPs and HACCP, FSMA Preventive Controls for Human Food, ISO 22000, and GFSI Recognized Standards with Risk Scoring Guidance in General Use with Fresh Produce

The intention of this qualitative research study was to provide a basic risk concept by comparing food risk assessment schemes and preparing general food risk scoring guidance for developing a simple and reliable practical fruit and vegetable qualitative food safety risk matrices for fresh produce entrepreneurs. These practical food safety risk matrices were verified using FDA fruit and vegetable food safety risk data. The FDA data were converted to a qualitative risk matrix referring to the fruit and vegetable 3 × 3 qualitative food safety risk matrix reference model. Other common qualitative risk matrix models, namely 3 × 3, 4 × 3, and 5 × 5, were constructed based on probability and severity scores for each hazard, as given in the FDA data. These were designated as practical fruit and vegetable 3 × 3, 4 × 3, and 5 × 5 qualitative food safety risk matrix models. The results of these models were compared with the fruit and vegetable 3 × 3 reference model. The two best compatible models are the 5 × 5 and 3 × 3 qualitative food safety risk matrix models. A preference test from focus group containing 12 participants showed good satisfaction overall, indicating that the practical fruit and vegetable 3 × 3, 4 × 3, and 5 × 5 qualitative food safety risk matrix models are useful for entrepreneurs. Understanding of basic risk concepts and verified scientific referencing of food safety risk matrices can improve entrepreneur’s risk assessment. This can be performed by using practical–scientific food safety risk matrices.

Trace Level Quantification of Organophosphorus Pesticides from Fleshy Fruit Samples by Magnetic Solid-phase Extraction Using Fabricated SBA-15/Fe3O4 Coupled with HPLC/UV

A mesoporous silica SBA-15/iron oxide (Fe3O4-SBA-15) nanocomposite was fabricated and employed as a magnetic solid-phase extraction (MSPE) sorbent to determine three types of organophosphorus pesticides (OPPs) in fruit samples. The fabricated nanocomposite was analysed using high-performance liquid chromatography with an ultraviolet detector (HPLC-UV). Meanwhile, the optimisation of extraction efficiency on three OPPs analyses used three different parameters, viz. desorption conditions, extraction time, and sorbent amount. The experimental results showed that the Fe3O4-SBA-15 nanocomposite achieved high analyte recoveries ranging from 89 to 118% with a relative deviation of less than 8.0%. By combining the optimised MSPE conditions with HPLC-UV, a suitable method for determining three OPPs was developed. This study revealed that the proposed method exhibits good coefficients of determination varying from 0.9942 to 0.9980, with low limit detection (LODs) ranging from 0.03 - 0.08 mgL-1 and low limit quantifications (LOQs) of 0.10 - 0.24 mgL-1. Based on these findings, the fabricated Fe3O4-SBA-15 nanocomposite is a suitable sorbent with excellent adsorption capacity for the selected OPPs from fruit matrices.

Untargeted metabolomics as a tool to monitor biocontrol product residues' fate on field-treated Prunus persica

Evidence of chemical plant protection products' (PPPs) long-term impact has been found in all environmental compartments. Therefore, other types of PPPs are developed to complement chemical PPPs like PPPs from natural sources, namely biocontrol products (BPs). Little is known about those new BPs, and it is important to assess their potential long-term environmental impact. Recently, the Environmental Metabolic Footprinting (EMF) approach was developed. It permits studying sample's entire meta-metabolome (endometabolome and xenometabolome) through a kinetics tracking of metabolomes of treated and untreated samples. Those metabolomes are compared time-by-time to estimate the “resilience time” of the samples after treatment. The current study aims to investigate BP residues' dissipation on peach fruits (Prunus persica). For that, an untargeted Liquid Chromatography-Mass Spectrometry metabolomics approach based on the EMF was optimised to separate the xenometabolome of the PPP from the endometabolome of the fruits. This “new version” of the EMF approach is able to target the BP treatment residues' (xenometabolome) dissipation exclusively. Thus, it is able to determine the time needed to have no more residues in the studied matrix: the “dissipation interval”. Field experiment was conducted on peach tree orchard against brown rot treated with (i) a plant extract BP (Akivi); (ii) a reference mineral extract BP (Armicarb®); and (iii) a Chemical reference treatment campaign. Formulated Akivi and its by-products' dissipation was monitored, a degradation kinetics appeared but the sampling did not last long enough to allow the determination of the “dissipation interval”. Armicarb® and the Chemical reference's residues and by-products showed a persistence pattern along the sampling kinetics. These results indicate that the EMF approach, formerly developed on soil and sediment, is applicable for fruit matrices and can be used to investigate the fate of complex BP treatment on the matrix through the xenometabolome tracking on treated fruits.

Influence of fruit-based beverages on efficacy of Lacticaseibacillus rhamnosus GG (Lactobacillus rhamnosus GG) against DSS-induced intestinal inflammation

Different lines of evidences from clinical, epidemiological and biochemical studies have established that optimal nutrition including probiotic and fruit phenolics can mitigate the risk and morbidity associated with some chronic diseases. The basis for this observation is the potential synergies that may exist between probiotic strains and different bioactive components of food matrices. This study was conceptualized to compare the efficiency of a probiotic strain in two different fruit matrices. Two fruits, viz., sea buckthorn (Hippophae rhamnoides) (SBT) and apples (Malus pumila) (APJ) were chosen and the anti-inflammatory effects of L. rhamnosus GG (ATCC 53103) (LR) fortified in SBT and APJ were analysed against dextran sulphate sodium (DSS) induced colitis in zebrafish (Danio rerio). The results showed that administration of probiotic (LR) fortified, malt supplemented SBT beverage (SBT + M + LR) had better restorative potential on the intestinal barrier function and mucosal damage, in comparison to LR fortified, malt supplemented APJ beverage (APJ + M + LR). SBT + M + LR demonstrated adequate anti-oxidant potential by enhancing the CAT, SOD, GPx and GSH activities, impaired due to DSS administration. The increase in the expressions of toll like receptor (TLR)-2, TLR-4 and TLR-5 induced by DSS were significantly inhibited by SBT + M + LR administration. Gene expression of pro-inflammatory markers, (NF-κB, TNF-α, IL-1β, IL-6, IL-8, CCL20, MPO and MMP9) were attenuated by SBT + M + LR treatment in intestinal tissues of DSS-treated zebrafishes. Notably, SBT + M + LR increased the expression of anti-inflammatory cytokine, IL-10. The study provides evidence that specific interactions between fruit matrix and probiotic strain can provide adjunct therapeutic strategy to manage intestinal inflammation.

Development of analytical protocol for the investigation of transformation products of pre-harvest fungicides in fruits using LC-MS/MS methods

ABSTRACT Chemical protection of plants is critical to permit all-year-long availability of plant products. These chemical agents are transformed both biotically and abiotically after spraying. Our purpose was to develop a workflow which is suitable to investigate those transformation products from plant matrices. Two field trials were set up in two years in two different plant matrices (apples, cherries) to develop a workflow to map the transformation products (TP) of three selected pre-harvest fungicides (boscalid, fluopyram, pyraclostrobin) in the fruits. Modified QuEChERS extraction method was applied for the extraction of TPs from the fruit matrices. We used liquid chromatograph-mass spectrometers to identify and confirm the transformation products of fungicides. LC-QTOF-MS method was suitable to map the key fragmentation routes of parent fungicides. Based on fragmentation pathways, MRM (multiple-reaction monitoring) transitions of fungicide-metabolites mentioned in the literature were predicted. HPLC-QTRAP-MS in target mode was successfully applied to monitor trace level of metabolites and in some cases their isomers. For confirmation of the identified metabolites, LC-QTOF-MS was used. Five earlier-documented as well as one novel transformation product (deschloro-FLP) were found in the investigated fruit samples, the latter has not been reported in plant matrices so far. Area-normalisation method was used to follow the relative concentration of the transformation products in the fruits as a function of time.

Optimalization of multiresidue method for the determination of pesticide residues in fruits

The main aim of this study was to optimize the QuEChERS methodology for the comprehensive determination of pesticide residues using a simple and inexpensive gas chromatography equipped with selective detectors working simultaneously (GC-ECD/NPD). The method was validated in accordance with the SANTE/11945/2015 guidelines. Representative fruit matrices were chosen from the commodities group with high water content (apples), and with high acidity and high water content (strawberry) for a 160 compounds in the range of 0.005−0.02 mg/kg. The accuracy of the method was as required (recovery: 70–120%, precision: below 20%) for 135 active substances in apples samples and 137 in strawberries samples. The main advantages of the methodology include: good detector response linearity (R2 above 0.995), low limits of detection (0.005 mg/kg for more than 20 compounds, 0.01 mg/kg for about 120 compounds), possibility of simultaneous determination of a wide range of compounds (including some “difficult” ones) without use of a single residue methods. The simultaneous use of two selective detectors allows to obtain more data during a single dose of the sample and confirmation of the result for most compounds, which allows to prevent false positives ones.

Fruits and fruit by-products as sources of bioactive compounds. Benefits and trends of lactic acid fermentation in the development of novel fruit-based functional beverages

Current awareness about the benefits of a balanced diet supports ongoing trends in humans towards a healthier diet. This review provides an overview of fruits and fruit-by products as sources of bioactive compounds and their extraction techniques, and the use of lactic acid fermentation of fruit juices to increase their functionality. Fruit matrices emerge as a technological alternative to be fermented by autochthonous or allochthonous lactic acid bacteria (LAB such as Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, and other Lactobacillus species), and also as probiotic vehicles. During fermentation, microbial enzymes act on several fruit phytochemicals producing new derived compounds with impact on the aroma and the functionality of the fermented drinks. Moreover, fermentation significantly reduces the sugar content improving their nutritional value and extending the shelf-life of fruit-based beverages. The generation of new probiotic beverages as alternatives to consumers with intolerance to lactose or with vegan or vegetarian diets is promising for the worldwide functional food market. An updated overview on the current knowledge of the use of fruit matrices to be fermented by LAB and the interaction between strains and the fruit phytochemical compounds to generate new functional foods as well as their future perspectives in association with the application of nanotechnology techniques are presented in this review.

Determination of phenolic acids and flavonoids from Myrciaria cauliflora edible part employing vortex-assisted matrix solid-phase dispersion (VA-MSPD) and UHPLC-MS/MS

In the present study, for the first time, vortex-assisted matrix solid-phase dispersion (VA-MSPD) is used to determine phenolic compounds in the edible part of Myrciaria cauliflora combined with ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) analysis. Under the optimized extraction conditions, the VA-MSPD method used a small amount of sample (25 mg), C18 (100 mg) as a solid support, a small volume of methanol (6 mL) as elution solvent and reduced time of grinding and vortex agitation (90 s). The proposed method showed good linearity (r ≥ 0.99) for all compounds, with detection limits in the range of 0.9˗99 μg kg−1. The matrix effect was between ˗0.8 and +52 %. This method allowed the quantification of four phenolic acids (4-hydroxybenzoic acid, gallic acid, p-coumaric acid and ellagic acid) and four flavonoids (myricetin, (+)-catechin, naringenin and quercetin) in M. cauliflora edible part harvested in different years. Ellagic acid was found with the highest abundance ranging from 470 to 709 mg kg−1. These results support the high potential of VA-MSPD to be applied in the determination of the phenolic compounds of fruit matrices, as well as, requiring a low solvent volume, a small amount of sample and solid support and features easy handling and short analysis time.