Abstract
ABSTRACT Chemical protection of plants is critical to permit all-year-long availability of plant products. These chemical agents are transformed both biotically and abiotically after spraying. Our purpose was to develop a workflow which is suitable to investigate those transformation products from plant matrices. Two field trials were set up in two years in two different plant matrices (apples, cherries) to develop a workflow to map the transformation products (TP) of three selected pre-harvest fungicides (boscalid, fluopyram, pyraclostrobin) in the fruits. Modified QuEChERS extraction method was applied for the extraction of TPs from the fruit matrices. We used liquid chromatograph-mass spectrometers to identify and confirm the transformation products of fungicides. LC-QTOF-MS method was suitable to map the key fragmentation routes of parent fungicides. Based on fragmentation pathways, MRM (multiple-reaction monitoring) transitions of fungicide-metabolites mentioned in the literature were predicted. HPLC-QTRAP-MS in target mode was successfully applied to monitor trace level of metabolites and in some cases their isomers. For confirmation of the identified metabolites, LC-QTOF-MS was used. Five earlier-documented as well as one novel transformation product (deschloro-FLP) were found in the investigated fruit samples, the latter has not been reported in plant matrices so far. Area-normalisation method was used to follow the relative concentration of the transformation products in the fruits as a function of time.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.