Source–sink balancing is crucial for a fruit load capacity in date palm trees. In date palm trees, coordination of suckers as sink strength with berries as source strength is very significant for economic yield production. The purpose of this study was to use the number of fruit bunches in two fruiting conditions of Off (less than 6 bunches) and On (more than 8 bunches) to identify the source-sink limitation in ‘Mazafati’ date palms with a normal fruit load (6≤bunch number≤8) to determine the optimal number of suckers, as active sinks that compete with berries, to be kept on a date palm. In this study, there were two groups, date palms with 4-5 suckers and date palms with 6-7 suckers. The results showed that the stress caused by 6-7 suckers (as compared to 4-5 suckers) limited source; reduced yield, bunch weight, fruit weight, size, and flavor; and increased fruit shedding and biennial bearing. Date palms with 6-7 suckers and a normal fruit load had a higher content of reducing sugars, a lower content of non-reducing sugars, and less starch in the organelles. They also showed higher trehalose metabolism responsive trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatases (TPP) genes expression along with lower carbon allocation sucrose-phosphate synthase (SPS1), sucrose synthase (SuSy) and invertase (INV) genes expression and enzymes activity in leaflets, which were in line with changes in yield and yield components. Therefore, the optimal number of suckers to be kept on a date palm with a normal fruit load was determined to be 4-5.