As a typical geographic landscape unit in the arid zone of northwest China, the distribution of the thermal environment in the substratum of the desert-oasis is of great significance to the monitoring of the ecological environment and the quality of human habitat in the region. The purpose of this research is to reveal the spatial–temporal evolution pattern of the thermal environment of the desert–oasis in the Turpan–Hami region from 2005 to 2020 and its trend changes, and to investigate the relationship between natural and human factors and the thermal environment and to conduct a long time series analysis. Firstly, the accuracy of MODIS land surface temperature (LST) data combined with site data was verified. Secondly, the mean-standard deviation method is used to identify desert heat island and oasis cold island zones. Again, the spatial–temporal distribution and change trends of thermal environment are explored by using standard deviation ellipse and spatial autocorrelation combined with land use/land cover (LULC) types. Finally, based on the multi-source remote sensing data, the natural factors and human factors are selected to explore their correlation with the thermal environment using Pearson correlation analysis. The results show that (1) the desert heat island zones are distributed in the desert areas on the periphery of urban built-up areas in Gaochang District, Shanshan County and Yizhou District of Hami Region. The oasis cold island zones are concentrated in the urban built-up areas of Gaochang District and Yizhou District, mainly because the vegetation coverage of urban built-up areas is higher than that of the peripheral desert areas. (2) The spatial development characteristics of the extremely high temperature (EHT) zone and the high temperature (HT)zone from 2005 to 2020 are “southeast-northeast-northwest” and “southeast-northwest” respectively, and the area of construction land 16a increased by 0.31%. This indicates that the spatial evolution of the thermal environment is closely related to the LULC and the degree of urbanization development. (3) From a four–period image with P < 0.001 and Z > 2.58, the thermal environment displays a high positive spatial correlation with Moran's I values of 0.45, 0.54, 0.47, and 0.45. (4) Temperature (Tem), downward longwave radiation (LWdown), and nighttime light intensity (NPP) all exhibited positive correlations with the LST and are significant in the desert region (p < 0.05); The albedo exhibited negative correlations with the LST and is significant in the grassland and woodland regions (p < 0.05).
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