Fresh Fish Research Articles

Fish freshness monitoring based on bilayer cellulose acetate/polyvinylidene fluoride membranes containing ZIF-8 loaded curcumin

This study presented a dual-layer freshness indicator film produced through electrospinning, combining cellulose acetate and polyvinylidene fluoride with zeolitic imidazolate framework-8 (ZIF-8) loaded with curcumin as the indicator. Our findings demonstrated that ZIF-8 effectively preserved its metal-organic framework structure during curcumin loading, ensuring the inherent color-changing ability of curcumin. The resulting colorimetric film exhibited altered tensile properties and increased water vapor permeability. Improved light stability and storage performance were observed. Compared to single-layer films, the dual-layer structure improved the hydrophilicity and stability of the indicator film. Importantly, the introduced indicator label efficiently captured the dynamic changes of TVB-N during freshness monitoring, providing comprehensive visual information for assessing fish freshness. The synergistic properties of ZIF-8, curcumin, and the dual-layer film structure contributed to an advanced freshness indicator system, providing a multifunctional and effective approach for real-time freshness assessment of fish freshness.

The effect of healthy eating on the development of stomach and colorectal cancer by the smoking and drinking status: Results from the Korean National Cancer Center (KNCC) community cohort study.

Determining the effect of dietary factors on cancer is a crucial issue when accounting for the effect of other major risks, such as smoking and drinking. A total of 15,563 adults from the Korean National Cancer Center Community Cohort were analyzed to determine and to compare the effect of dietary factors on stomach and colorectal cancer in overall and in the subgroup of non-smokers (or urinary cotinine concentrations <5 ng/mg) and non-drinkers with Cox proportional-hazard models. During the mean follow-up (13.7 years), 469 and 299 cases of stomach and colorectal cancer were identified, respectively. The preventive effect of vegetable, fish, and soybean/tofu intake on colorectal cancer was found in women after adjustment for smoking, drinking, BMI, and sociodemographic factors. In the subgroup analysis of non-smokers and non-drinkers, the effect on colorectal cancer was increased in women (≥1 time/week vs. almost never, vegetables: hazard ratio (HR) 0.30, 95% confidence interval (CI) 0.13-0.69; fish: HR 0.46, 95% CI 0.26-0.83), and the fresh fish intake effect on stomach cancer was newly identified in men (HR 0.36, 95% CI 0.15-0.86). These effects were more pronounced and additionally shown in other dietary factors such as soybean or tofu in women and vegetables and fish in men, when subjects with <5 ng/mg urinary cotinine concentrations applied. The protective effect of healthy eating on the risk of stomach and colorectal cancer were different by smoking and drinking status. Rigorous control of smoking and drinking effects is necessary when measuring the effect of dietary factors on cancer, properly.

Optimalisasi Pengembangan Bisnis pada Petani Ikan Ciparay

An essential strategic effort to improve community welfare and the productivity of the fishing industry is to optimize the development of fish farmer businesses in Ciparay, Bandung Regency, West Java, which has a lot of potential for cultivating freshwater fish. Fish farmers in this area, however, still face several problems. These include lack of access to advanced technology, knowledge about managing a business, and market limitations. This research aims to find and create the most effective strategy for building a fish farming business in Ciparay. This research uses SWOT analysis (Strengths, Weaknesses, Opportunities, and Threats) and Business Model Canvas to find internal and external elements influencing the fish farming business. Apart from field observations and in-depth interviews with fish farmers, data was collected through relevant literature studies. The results of the analysis show that traditional knowledge and extensive experience in fish cultivation are the main strengths of Ciparay fish farmers. In contrast, the main weakness is the difficulty of obtaining technology and capital. The ever-increasing market demand for fresh fish and government support through various aid programs are opportunities that can be exploited. However, there are threats such as climate change and competition with fish products from other countries. The results show that there are several methods that Ciparay fish farmers can use to maximize their business growth. First, increasing product diversification and innovation in fish processing can open up new market opportunities and increase product-added value. Second, increasing access to modern technology through training and technical assistance can help improve product quality and productivity. Third, forming farmer groups or cooperatives can strengthen farmers' bargaining positions and facilitate their access to capital and wider markets. Farmers can gain greater access to markets and capital by implementing stratification strategies

An intelligent myofibrillar protein film for monitoring fish freshness by recognizing differences in anthocyanin (Lycium ruthenicum)-induced color change

A novel smart film MP/BNC/ACN for real-time monitoring of fish freshness was developed using myofibrillar protein (MP) and bacterial nanocellulose (BNC) as film raw materials and anthocyanin (Lycium ruthenicum, ACN) as an indicator. Firstly, the film containing 1 % ACN (MP/BNC/ACN1) was found to have a moderate thickness (0.44 ± 0.01 mm) and superior mechanical properties (tensile strength (TS) = 8.53 ± 0.11 MPa; elongation at break (EB) = 24.85 ± 1.38 %) by determining the physical structure. The covalent, electrostatic, and hydrogen bonding interactions between anthocyanin and the film matrix were identified and confirmed by FT-IR spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM) analysis. A comprehensive evaluation concluded that MP/BNC/ACN1 exhibited excellent trimethylamine (TMA) sensitivity (total color difference (ΔE), ΔETMA0-1000 = 4.47–31.05; limit of detection (LOD), LOD = 1.03) and UV stability (ΔE96h = 4.16 ± 0.13). The performance of the films in assessing fish freshness was evaluated, principal component analysis (PCA) and hierarchical cluster analysis (HCA) revealed that MP/BNC/ACN1 (ΔE2-10d = 16.84–32.05) could clearly distinguish between fresh (0–2 d), sub-fresh (4–6 d), and spoiled (8–10 d) stages of fish, which corresponded to the film colors of red, light red, and gray-black. In conclusion, this study addresses the limitation that intelligent films cannot visually discern real-time freshness during fish storage and provides a promising approach for real-time fish freshness monitoring.

Pendampingan dan Penguatan Kapasitas Masyarakat dalam Pemulihan Kerusakan Lingkungan di Provinsi Sulawesi Barat

Community empowerment begins with understanding that every individual and group has potential and abilities that can be developed. In Mamuju Regency, precisely in Tommo District, there is peat but it is small which if damaged and not managed properly will result in land fires, floods, and soil pollution. This assistance aims to position the community as a party that plays an active role in environmental recovery. One of the initial stages of assistance or empowerment activities carried out is Problem Identification and Situation Analysis. It is hoped that the problems faced can be clearly identified, the related situation can be analyzed comprehensively, and the next steps can be designed to plan effective and sustainable interventions. Strengthening the understanding of problem identification and location analysis is carried out using the IMAS tool through survey methods, interviews, and FGDs. The results of problem identification and situation analysis found several problems that can be used as references in formulating systematic and effective planning for community empowerment. Based on the results of the problem identification and situation analysis in Buana Sakti Village, Tamemongga Village, Tamejarra Village, West Leling Village and North Leling Village, problems were found in flooding, lack of fertilizer availability and the distance of the village from the city which makes it difficult to get fresh fish to meet the protein needs of the community. From the results of the problem identification and situation analysis, a Community Work Plan formulation was formed such as freshwater fish farming, long-term crop planting, horticultural planting, and livestock farming. The proposed work plan is expected to be able to help the community in meeting needs, improving the community's economy, and can answer problems that occur in each village.

Fish species authentication in commercial fish products using mass spectrometry and spectral library matching approach

Seafood fraud has become a global issue, threatening food security and safety. Adulteration, substitution, dilution, and incorrect labeling of seafood products are fraudulent practices that violate consumer safety. In this context, developing sensitive, robust, and high-throughput molecular tools for food and feed authentication is becoming crucial for regulatory purposes. Analytical approaches such as proteomics mass spectrometry have shown promise in detecting incorrectly labeled products. For the application of these tools, genome information is crucial, but currently, for many marine species of commercial importance, such information is unavailable. However, when combining proteomic analysis with spectral library matching, commercially important fish species were successfully identified, differentiated, and quantified in pure muscle samples and mixtures, even when genome information was scarce. This study further tested the previously developed spectral library matching approach to differentiate between 29 fish species from the North Sea and examined samples including individual fish, laboratory-prepared mixtures and commercial products. For authenticating libraries generated from 29 fish species, fresh muscle samples from the fish samples were matched against the reference spectral libraries. Species of the fresh fish samples were correctly authenticated using the spectral library approach. The same result was obtained when evaluating the laboratory-prepared mixtures. Furthermore, processed commercial products containing mixtures of two or three fish species were matched against these reference spectral libraries to test the accuracy and robustness of this method for authentication of fish species. The results indicated that the method is suitable for the authentication of fish species from highly processed samples such as fish cakes and burgers. The study shows that current and future challenges in food and feed authentication can efficiently be tackled by reference spectral libraries method when prospecting new resources in the Arctic.

A pH-sensitive film based on chitosan/gelatin and anthocyanin from Zingiber striolatum Diels for monitoring fish freshness

As a new type of packaging method, the anthocyanin-based pH-sensitive indicator film has gained much attention owing to low cost, small size, and visually informative property. In this study, an intelligent film based on chitosan/gelatin (CG) matrix with Zingiber striolatum Diels (ZSD) anthocyanin for fish freshness monitoring was developed. The film properties, including thickness, moisture content, color, mechanical properties, UV–vis light barrier property, as well as pH and ammonia sensitivity, were evaluated. The CG-ZSD films exhibited a more compact structure when compared with the CG film. The CG-ZSD20 film showed the highest elongation at break (6.33 ± 0.62%) and lowest tensile strength (20.0 ± 0.58 MPa). FTIR spectra revealed the strong hydrogen bond interactions between ZSD and polymer matrix. Film incorporated with 15% anthocyanin extract has increased melting temperature at 118.9 °C, and a lower weight loss (13.8%) at melting temperature. In pH 1–14 buffer, the color of CG-ZSD films underwent a significant change from red to yellow-green. The CG-ZSD15 film was utilized for monitoring fish freshness and showed visible color changes from deep purple to brown. The total volatile basic nitrogen content and pH value changes of fish were closely related to the visual color changes in film. This demonstrated that the film was a highly pH-sensitive film for quantifying fish freshness in real-time.

Study on the Toxic Effects of Deltamethrin on Hematological Parameters of a Fresh Water Fish, Common Carp (Cyprinus carpio L.)

Fish health is adversely affected by pesticide contamination, which is a significant cause of pollution in freshwater ecosystems. One helpful technique for assessing the general health of different fish species is the analysis of blood parameters. Present study investigated the detrimental effects of deltamethrin on different hematological parameters of a fresh water fish, common carp (Cyprinus carpio). An experiment with a 28-day exposure to 1/20th and 1/10th of the 96-hour LC50 value (0.114µl/L) was conducted in order to investigate the effects of sub-lethal concentrations. On the 7th, 14th, 21st, and 28th day of exposure, several hematological parameters like Total Erythrocyte Count (TEC), Total Leukocyte Count (TLC), Hemoglobin (Hb), Hematocrit/Packed Cell Volume (Hct/PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) were analyzed in comparison to control. After the exposure of different concentrations of deltamethrin significant decline (P*&lt;0.05 and P**&lt;0.01) in Hb, TEC, PCV and MCHC was noted as compared to control. On the contrary TLC, MCV and MCH showed increasing trend after the treatment of pesticide. This study showed that deltamethrin considerably altered the hematological markers of fish, even at sub-lethal concentrations.

Pelatihan Pemasaran Digital Tokopedia Dalam Upaya Peningkatan Penjualan Kelompok Binaan Dinas Perikanan Cilacap

The level of success of collaboration can be achieved when the collaboration involves people with diverse experiences, multi-disciplinary knowledge, different cultures, education and backgrounds. Various parties will present different thoughts and contributions, so that the problem will be handled from various angles in a comprehensive manner. The problem in Cilacap Regency, with the majority of coastal communities working as fishermen and fish processors, is that sales are stagnant because they only rely on traditional marketing, by selling fresh fish at TPI Padanarang or processing fish into crackers, salted fish, handicrafts that are sold directly at around TPI Pandanarang. The aim of this PkM is providing digital marketing training through Tokopedia as an effort to increase sales of groups supported by the Cilacap Fisheries Service. The method chosen was qualitative with the number of respondents being 10 residents representing groups supported by the Cilacap Fisheries Service. The results of the PkM which was at TPI Pandanarang - Teluk Penyu Area, South Cilacap District, Cilacap Regency were the creation of 10 Business Identification Numbers (NIB) and 10 online shop accounts on Tokopedia for 10 people representatives of groups supported by the Cilacap Regency Fisheries Service. Conclusions from field observations, apart from marketing training, it turns out that the community needs a lot of assistance when processing permits such as PIRT, halal certificates, promotional training, packaging design, hygiene and sanitation training, health safety food. The Cilacap District Fisheries Service can collaborate with several companies/agencies to get assistance with equipment grants, training from Corporate Social Responsibility (CSR) funds.

Microplastics contamination in commercial fish meal and feed: a major concern in the cultured organisms

The growing scale of plastic pollution causes a devastating impact on the aquatic ecosystem. The people largely depend on animal-based food for their protein requirements. In this study, we analysed 10 different fish meal samples and 20 feed samples used in farming to understand the level of microplastic (MPs) contamination and estimate the amount of MPs ingested by farmed fish, shrimp, and chicken through feed. The abundance of MPs in fish meal samples ranges from 210 ± 98.21 to 1154 ± 235.55 items/kg. The fish meal produced from dried fish is more prone to MPs contamination than that produced from fresh fish. In the case of fish feed, MP abundances range from 50 ± 22.36 to 160 ± 36.57 items/kg in shrimp feeds, 60 ± 26.74 to 230 ± 52.32 items/kg in fish feeds and 90 ± 25.11 to 330 ± 36.12 items/kg in chicken feeds. The exposure rate of MPs is higher in the grower- and finisher-stage feeds than in the starter feed. Fiber-shaped MPs of size 100–500 μm with PE and PP polymers were predominantly found in fish meal and feed samples. EDAX analysis showed the presence of Cr, Cd, Ti, Ni, Cu, As, Al, Pb, Hg, Cd, Ti, Fe, Ca, K, and Si in fish meal samples and Ca, Na, Zn, Cu, Ni, Cl, Al, Si, S, Pb, Cd, Ti, Cr, Mg and Fe in feed samples. The possible level of exposure of microplastic particles was calculated based on MP contamination in feed, feed consumption rate, and body weight. We estimated an MP exposure level of 531-1434 items/kg feed for farmed shrimp, 234–4480 items/kg feed for fishes, and 3519–434,280 items/kg feed for chicken. This study concludes that fish meal and feed are one of the important exposure routes of MPs to the farmed animals.

Visual tracking of real-time freshness of fish using an agar hydrogel colorimetric indicator containing CuNPs/NCQDs

A simple, selective, and affordable dual fluorescence-colorimetric indicator for hydrogen sulfide was developed based on a complex of copper nanoparticles and N-doped carbon quantum dots (CuNPs/NCQDs). Real-time and visual freshness tracking of fish was done using a colorimetric indicator by incorporating CuNPs/NCQDs into agar hydrogel (AH-CuNPs/NCQDs). The fluorescence response of the CuNPs/NCQDs solution is quenched upon exposure to H2S. The field-emission scanning electron microscopy image of the AH-CuNPs/NCQDs film revealed a unified structure. The prepared indicator exhibited a good and irreversible response to H2S, with a LOD of 91.36 and a LOQ of 276.86 μM, based on the localized surface plasmon resonance (LSPR) mechanism. The X-ray photoelectron spectrometer and Fourier transform infrared spectrometer results confirmed the formation of a CuS bond in the colorimetric indicator exposed to fish spoilage. The prepared indicator demonstrated good stability and remained unaffected by pH or other volatile compounds. Notably, there was a strong correlation between ΔΕ and fish freshness parameters (pH, TV-BN, and TVC). Light green, pale yellow, and dark yellow colors, respectively, indicated freshness, semi-freshness, and spoilage of fish during storage in the refrigerator. Overall, the prepared indicator can be effectively used for detecting spoilage in meat products as a highly sensitive freshness indicator.

Risk Assessment and Heavy Metals Accumulation in Organs of Clarias Gariepinus and Heterobranchus Longifilis from Omambala River, Anambra State Nigeria

Heavy metals are toxic to organisms even at low concentrations. In recent times, there has been the high occurrence of cancer cases locally, traceable to heavy metal contaminations of foods consumed by man and animals. The experimental site for this study was Omambala River which serves as a source point of many fresh fishes as well as its distribution throughout the state both in dry and rainy seasons. One hundred (100) samples each of Clarias gariepinus and Heterobranchus longifilis, with the size of 17–21 cm were selected from Omambala River. Heavy metal determination was done using the digestion method. All the digested samples were analyzed three times for heavy metals using SP-V21A Vis Spectrophotometer and the instrument was calibrated with standard solutions. For Risk assessment analyses, the target hazard quotients (THQ) index was applied to assess the potential non-cancer risk associated to consumption of the different species of marine organisms sampled. While the total target hazard quotient (TTHQ) was calculated as the arithmetic sum of each THQ value. The lifetime cancer risk (TR) was calculated by multiplying the daily dose by the cancer slope factor (CSF) derived by response-dose curve for toxicant ingestion. The bioaccumulation factor (BAF) was determined by dividing the metal concentration in the tissue of the organism by the metal concentration in the environment or medium. From the correlation analysis, there was a positive correlation between the four different organs of the fish species and the selected heavy metals. The toxicity of magnesium and zinc are higher in C. gariepinus while the toxicity of iron is higher in H. longifilis. The degree of toxicity is zinc &gt; iron &gt; magnesium &gt; lead &gt; cadmium &gt; copper &gt; mercury &gt; chromium. The water quality index (WQI) for all the heavy metals in four different organs of the two fish species was found to be less than 1 (WQI &lt; 1) meaning low pollution; which further means that the consumption of C. gariepinusand H. longifilis may not pose any health risk to the people of the area. However, there are reports of lifetime cancer risk on daily consumption of Chromium, Cadmium, Lead and Mercury in fish. Chemicals when found in large quantities.

Efikasi Bakterostatik Kulit Bawang Merah Pada Ikan Bandeng (Chanos chanos) Selama Penyimpanan Suhu Ruang

Biochemical and physicochemical changes result in a decrease in fish freshness. The decline in quality of fresh fish can be influenced by several things, one of which is storage temperature, where the higher the storage temperature, the faster the fish rots. Red onion skin contains flavonoid compounds which function as bacteriostatics, namely quercetin. The aim of this research is to provide scientific information about the bacteriostatic efficacy of shallot skin on histamine inhibition in milkfish during room temperature storage. The research design used was a Randomized Block Design (RAK) with three treatment factors with two replications. Based on the research results, it was found that it showed the best concentration and had a significant effect on the quality (pH, histamine and TPC) of crushed milkfish during 48 hours of storage at room temperature with the addition of 3% shallot skin. The results of the organoleptic test showed that the red onion skin of crushed milkfish experienced a decrease in organoleptic values for each parameter (Appearance, Smell, Texture) for 48 hours so it was necessary to increase the concentration of red onion skin.

Morphometric and molecular study on Capillarids infection in some fresh water fishes

Morphometric and molecular study on Capillarids infection in some fresh water fishes

Identification of Pathogenic Bacteria in Fresh and Smoked Fish of the Tuna (Thunnus sp.) with SNI 01-2332.2-2006

Identification of Pathogenic Bacteria in Fresh and Smoked Fish of the Tuna (Thunnus sp.) with SNI 01-2332.2-2006

Effect of Different Processes to Nutrient Profile of Dead Fresh Fish Carangoides armatus as Potential Protein-Rich Seafood

Effect of Different Processes to Nutrient Profile of Dead Fresh Fish <i>Carangoides armatus</i> as Potential Protein-Rich Seafood

PREVALENCE OF ECTO AND ENDO PARASITES OF WHITE CATFISH (AMEIURUS CATUS) IN UKE RIVER, KARU LOCAL GOVERNMENT AREA OF NASARAWA STATE, NIGERIA

In Uke River, Karu Local Government Area, Nasarawa State, Nigeria, a six-month research was carried out between January and June 2023 to examine the gills and intestinal helminth and protozoan parasites of White Catfish (Ameiurus catus). A total of 100 fresh Ameiurus catus fish samples of both sexes, were obtained from the fishermen and conveyed to the Zoology laboratory of Nasarawa State University for examination. The fishes were processed and examined using gills, oesophagus, intestine and rectum. Twenty-two (22) out of 100 fishes were found to be infected with helminth and protozoan parasites and a total of 31 parasites were isolated belonging to protozoa (Eimeria spp, Hexamita spp, Protoopalina spp), nematode (Procamallanus spp), trematode (Dactylogyrus spp), Cestode (Diphyllobothrium latum, Bothriocephalus claviceps) taxonomic groups. The findings indicated that Ameiurus catus intestine contained a greater quantity of fish parasites (96%). A nematode (Procamallanus spp.) was found to have the highest prevalence of 45.16%. Although the Chi square results showed no significant difference (p&gt;0.05), the prevalence in Ameiurus catus was greater in female samples (63.64%) compared to male samples (36.36%). Fish weighing between 151-200 g (46.14%) and measuring between 21-30 cm (28.57%) had the highest percentage of parasite infection. Going forward, it is imperative to properly cook Ameiurus catus from the Uke River to prevent the spread of parasites to consumers. Additionally, it is advisable to steer clear of any potential actions that could contribute to the parasite predominance near the water body.

Detection of Salmonella and Escherichia coli along the Fish Value Chain in Bahir Dar City, Ethiopia

ABSTRACTBackgroundFish is a possible source of foodborne infections with Salmonella and Escherichia coli. This study was conducted to identify Salmonella and E. coli along the fish value chain in Bahir Dar City, Ethiopia.MethodsA cross‐sectional study was undertaken with purposive sampling. A total of 121 specimens comprising fresh fish, retailing fish, filleted and cooked fish, swabs, and water samples were collected. Both culture based and molecular methods were used for detection.ResultsE. coli isolated from 41 (33.88%) and Salmonella from 6 (4.96%) specimen. The highest E. coli isolation rate was from retailing fish 16 (80%), whereas the highest Salmonella isolation rate from filleted tissue 2 (20%). At restaurants, 12 (30%) samples were positive for E. coli and 3 (7.50%) for Salmonella. All 41 E. coli isolates were resistant to amoxicillin/clavulanate, whereas no resistance was shown for gentamicin and amikacin. Two thirds of Salmonella isolate and 95.12% of E. coli were detected as they develop multidrug resistance. The highest rate of resistance was recorded for ceftazidime against all (n = 6) isolates of Salmonella species. From a total of 10 E. coli isolates tested by PCR, 4 were positive for hemolysin A1 and/or eae virulence genes.ConclusionsThe study detects the potential biological hazards along the value chain. Hygiene of fish handlers and their working environment and proper fish cooking are highly valuable. One health campaign should be carried out on drug resistance, contamination of the lake, and fish safety.

In-package cold atmospheric plasma processing for shelf-life extension of gilthead seabream (Sparus aurata) fillets.

In-package cold atmospheric plasma (CAP) processing, which refers to the generation of CAP inside a sealed package, enables a local disinfecting reaction, allowing no post-process contamination and extending the shelf-life (SL) of perishable food products, such as fresh fish. In the present study, four in-package CAP treatments, differing in frequency and processing time, were applied on fresh gilthead seabream (Sparus aurata) fillets, prepacked in low-permeability pouches. Fish SL was evaluated during isothermal storage at 2°C, whereas untreated packaged fillets were used as control samples. The SL assessment of the fish fillets was based on microbial enumeration of total aerobic mesophilic count (TMC), total aerobic psychrotrophic count (TPC), Pseudomonas spp., Enterobacteriaceae, and lactic acid bacteria (LAB), pH measurement, determination of color and texture parameters, lipid oxidation, total volatile basic nitrogen (TVB-N) measurement, and sensory evaluation. All CAP treatments were effective against microbial inhibition in fish fillets, especially regarding TMC, TPC, and Pseudomonas spp., resulting in maximum reduction of 1.49, 1.24, and 1.43logCFU/g, respectively, compared to the control samples after 16 days of storage. However, minor effect was observed against Enterobacteriaceae and no effect against LAB. CAP processing did not affect the color and texture parameters of fish fillets, and TVB-N production was slightly reduced in CAP-treated samples; however, lipid oxidation was accelerated, especially at the more intense processing conditions, by a maximum of 75.5%. The results of the study indicated that in-package CAP processing could be effectively applied for inhibiting spoilage during refrigerated storage and extending SL of fresh fish fillets. PRACTICAL APPLICATION: In-package cold atmospheric plasma (CAP) processing was tested on gilthead seabream fillets, a highly perishable product with high commercial potential if its shelf-life can be extended through minimal processing. The food industry could benefit from in-package CAP technology as it is a cost effective nonthermal processing method while preventing post-processing contamination of the products. Although in-package CAP processing has not been extensively tested on fish, this study examined the quality and shelf-life of a highly perishable fish species, and the results could be further used as a reference for processing optimization of the CAP treatments.

Kelembagaan dan Bauran Pemasaran Hasil Tangkapan Nelayan di Tempat Pelelangan Ikan (TPI) Desa Sumberanyar Kecamatan Paiton Kabupaten Probolinggo

Research on institutions and marketing of fishermen's catches at fish auctions. The background of this research is to introduce TPI Paiton to the community, to increase fishermen's income and fishermen's welfare in Sumberanyar Village, Paiton District, Probolinggo Regency. The purpose of this research is to determine the level of utilization and the need for basic facilities, functional facilities, and supporting facilities. To find out the application of 4P in the process of selling fish at TPI Paiton, Sumberanyar Village, Paiton District. The research was conducted in Paiton District using the Slovin method. The analytical method used is a qualitative approach. The results of the discussion showed that the condition of the facilities at TPI Paiton, both basic facilities, functional facilities, and supporting facilities were still all functioning and used by fishermen. The application of 4P at TPI Paiton is product, where the products at TPI Paiton are salted fish and fresh fish, then the price or price where the price set by traders at TPI Paiton is based on the purchase price of fish per kilo and customer prices. Place or a place where the place offered by the producer is still only a few places such as traditional markets, out of town, factories, and in their own homes. Promotions or promotions carried out at TPI Paiton have been effective by selling fish in traditional markets, outside the city, factories and in their own homes.