Simple SummaryMilk produced in Alpine farms under pasture- and-grass-based feeding systems is characterized by beneficial nutritional traits, which are linked to its high fat quality. In this study, milk samples collected in two alpine farms set in the Italian Piedmont region were analyzed together with samples of feedstuffs (pasture, fresh grass, concentrates and total mixed ration) furnished to cows during summer and winter, when the feeding strategy was modified. The results suggested a favorable fat composition of all samples analyzed, with some differences detected between the seasons and the farms. The milk obtained following a pasture- or fresh-grass-based feeding strategy (during summer) showed a higher fat quality, which was characterized by higher amounts of beneficial fatty acids. Particularly, a distinctive fat composition of milk samples coming from cows exclusively fed on pasture during the summer season was evidenced. The outcomes obtained in this study contributed toward evaluating and promoting alpine dairy products from the Alpine region as products associated with an added value, with beneficial effects for both producers and consumers.The production systems linked to mountain animal husbandry have had an environmental, social and cultural role in recent years. Zootechnical systems based on feeding strategies, such as pasture grazing and grass-fed strategies, contribute to a significant increase in the relative amounts of favorable fatty acids (FAs) in animal products, indicating their ability to improve the long-term health of consumers. In this study, we compared different feeding strategies in two small mountain farms in the Piedmont Alpine region, Italy. Particularly, during the summer season, the two farms were distinguished by the exclusive employment of Alpine pasture (farm A), assumed as the best way to improve the quality of the FA profile in milk vs. the supply of daily fresh cut mountain grass plus a reduced implementation with hay and concentrates directly in the barn (farm B). The milk fatty acid profile was analyzed using gas chromatography. The results showed the high quality of alpine milk collected in the two farms. Even with some differences, particularly evidenced when comparing the summer diets, the milk FA profiles in farm A and farm B were favorable from a nutritional point of view in both seasons. Milk samples obtained using the exclusive employment of alpine grazing during summer were represented by an FA profile of higher quality (lower saturated FAs, higher branched FAs and monounsaturated FA, favorable n6/n3 ratio). However, milk obtained using the integrated strategy (fresh grass plus concentrates in the barn farm B) resulted in a more homogenous composition during the summer season, with a higher concentration of polyunsaturated FAs. These outcomes suggested that the integrated strategy, even if related to a lower ability in improving milk FA profile, could represent a valid and cost-effective alternative for mountain farmers to obtain an overall superior quality of milk, which was not strictly linked to the grazing practice. The multivariate analysis showed that information contained in the milk FA profile may provide a valuable tool that can distinguish mountain-grass-based diet.