Fresh-cut Carrots Research Articles

カットニンジンのエチレン処理による苦味発現の誘導ならびに環境ガス組成変更による制御

カットニンジンのエチレン処理による苦味発現の誘導ならびに環境ガス組成変更による制御

Regulation of Glycolytic Metabolism in Fresh-cut Carrots under Low Oxygen Atmosphere

Carrot (Daucus carota L.) shreds were stored under a continuous flow of air or 0.5% and 2% O2 (balance N,) for 9 days at 5 and 15C. The resulting changes in respiration, levels of glycolytic intermediates, and activities of ATP: phosphofructo kinase (ATP-PFK), and PPi: phosphofructokinase (PPi-PFK) were monitored. Carrots under low O atmosphere exhibited an increase in RQ due to a greater reduction in 02 consumption than in CO2 production, and the increase in RQ was greater at 0.5% than at 2% O2 at both temperatures. Fructose 1,6-bisphosphate (F1,6P) accumulated with decreased O2 atmosphere and was 2-fold greater at 0.5% than at 2% O2 atmosphere at both temperatures. The levels of other glycolytic intermediates were not significantly influenced by low O2. The increase in PPi-PFK activity occurred at the same time as F1,6P accumulation. A similar relationship was not found with ATP-PFK. These results suggest that PPi-PFK may be involved in regulation of glycolysis under low O2 atmosphere.