Freeze-thaw Research Articles

Intracellular hydrogen peroxide’s effect on quality parameters of rooster sperm in freeze/thaw cycle

Relevance. One of the factors decreasing total motility after cryopreservation rooster’s sperm is influence of reactive oxygen species. Morphological and biochemical features of avian spermatozoa structure, which make them more susceptible to cryopreservation process compared to mammalian spermatozoa, may also be the reason why avian spermatozoa are more susceptible to oxidative stress.Methods. The purpose of the study is to trace the change in the level of intracellular reactive oxygen species, in particular hydrogen peroxide (H2O2), during the cryopreservation of rooster sperm, to assess its effect on the quality and viability of sperm.Results. A negative correlation (r = -0.68, p < 0.05) was found between the intracellular level of hydrogen peroxide and the number of dead cells in native sperm. In the frozen (thawed) seed, a weak relationship was observed between these indicators (r = -0.10). There was a significant effect of the level of intracellular hydrogen peroxide in freshly obtained ejaculates on the overall mobility of frozen (thawed) semen (r = -0.65, p < 0.05). This allows us to assume that, similarly to mammalian spermatozoa, the production by cells of an increased amount of reactive oxygen species (H2O2) in the freezing (thawing) cycle negatively affects the functional status of mitochondria, which, as is known, are the main source of energy for the sperm, ensuring the operation of the kinetic apparatus of the sperm and its general mobility. Data were obtained on the individual variability of the hydrogen peroxide content in the sperm of roosters at the age of 61 weeks in the freezing (thawing) cycle, allowing the selection of roosters according to this indicator

Experimental study on the effect of freeze–thaw cycles to the cohesion and moisture content of geogrid reinforced silty clay

The freezing and thawing cycle is one of the primary causes of damage and instability to buildings in seasonal frost regions. During this process, the mechanical properties of soil are affected, leading to settlement, cracking, or deformation of infrastructure. Mitigating or reducing the occurrence of building frost damage in seasonal frost regions has become an important subject of study. Freeze–thaw (F–T) action will influence the distribution of moisture inside the reinforced soil and change the strength of thawing soil, which is closely related to the main influencing factors, such as initial moisture content, compaction degree, reinforced spacing, number of freeze–thaw cycles (FTC), freezing temperature, and effective vertical stress. Cohesion is an important index to determine the shear strength of clay, which is important to analyze the change in cohesion after F–T. Meanwhile, cohesion is closely related to soil moisture content. This study conducted orthogonal experiments on these primary influencing factors (6 factors at 5 levels) through FTC tests, triaxial tests, and moisture content tests to determine the undrained cohesion and moisture content of the clay after FTC, thereby establishing the influence of reinforcement on soil strength under freeze–thaw conditions. Based on the experimental results, SPSS software was used to fit the regression equations of undrained cohesion and moisture content expressed by the main influencing factors at different heights of the clay. Optimization options for the main influencing factors were obtained with Matlab software when the highest undrained cohesion values 6.8, 10.6, 8.9 kPa and lowest moisture content values 24.0%, 24.3%, 26.2% appeared in upper, middle and lower parts of the testing clay structure respectively, in conditions of − 15 °C freezing temperature and 5 times FTC. And determined the optimal combinations of moisture content, reinforcement spacing, compaction density, and vertical load at different heights. Decreasing reinforced spacing in silty clay was beneficial for liquid underwater seepage after F–T. The redistribution of internal moisture in the soil sample strengthened its undrained cohesion, thereby increasing the soil's shear strength. Comparing reinforcement conditions at different locations, it was found that when there were 3 layers of reinforcement with a spacing of 150 mm between them, this spacing was optimal. It played a significant role in improving the soil's shear strength and enhancing its bearing capacity. For reinforced clay itself, the order of the main factors influencing the undrained cohesion of soil after F–T, from high to low, was initial moisture content, reinforced spacing, and compaction degree.

Theoretical porosity design, mechanical properties, and durability of large-pore sandy recycled concrete

This study used recycled aggregate (RA) and mineral additions to design large-pore sandy recycled concrete (LPSRC) using a design porosity (DP) of 25 %, 20 %, and 15 %. Thus, the related effects of recycled coarse aggregate (RCA), recycled fine aggregate (RFA) and fly ash (FA) on the compressive strength, frost resistance (using slow and fast freezing methods), and sulfate attack properties of LPSRC were systematically studied. Moreover, to reduce the error between the design and theoretical porosity values, the bulk density ratio adjustment method was employed to design the theoretical porosity. The results of binarization analysis demonstrated that the deviation of the actual porosity caused by the sidewall effect was ∼2 %, which should be considered during DP design and performance evaluation. The compressive strength of the LPSRC after 56 curing days increased by 35.9 % on average as the DP was reduced from 25 % to 15 %. In a freeze–thaw cycle test, the slow freezing method was more accurate for evaluating the frost resistance of the LPSRC. Under the same conditions, the mass loss rate of the LPSRC calculated using the slow and fast freezing methods was 3.2 % and 7.62 %, respectively. This distinction is pivotal for establishing reliable frost resistance criteria for LPSRC in cold-weather applications, complementing previous research in the field. In terms of sulfate deterioration resistance, compressive strength corrosion factors decreased with increasing DP. Additionally, damage was mainly concentrated at the contact interface between RCA and the cement matrix, while the aggregate itself showed relatively little damage, suggesting RCA application has a positive effect on improving preparation of the LPSRC. Specifically, as DP increased from 15 % to 25 %, the average reduction in the compressive strength corrosion factors for LPSRC after 30 and 60 times of dry–wet cycle were 9.64 % and 8.37 %, respectively, illustrating a clear correlation between higher DP and reduced sulfate resistance. This study contributed to the LPSRC design and production using RCA, RCF and FA, that positively impacts to the sustainable development of the construction industry and helps to evaluate the effectiveness of RA in macroporous concrete. Moreover, the strategic implementation of design porosity has been shown to optimize the structural integrity and performance of the concrete, achieving a balance between strength and workability, which is crucial for the concrete's durability and its ability to adapt to various environmental conditions.

Adjusting the hydrophobicity of recombinant microgels to improve interfacial activity: Using a promising pickering emulsion stabilizer with a dense cubic structure

Developing recombinant microgels (RMs) with high mechanical and anti-coalescence properties provides a safe and economical method for constructing uniform and stable Pickering emulsions. Previously, we prepared RMs based on polysaccharides using freeze–thaw cycles, displaying excellent stability. Because RMs can be formed between polysaccharides, we studied whether they can be formed between polysaccharides and proteins for preparing Pickering emulsions. Therefore, we report a heat-induced gelation method to generate RMs with bovine serum albumin (BSA) and κ-carrageenan (KC). Owing to heat-induced treatment (90 °C), the high hydrophilicity of KC could be regulated by the exposed hydrophobic groups of BSA, and the system rapidly gelated after cooling (−18 °C) to obtain a dense recombinant structure. The TPA and SEM results further demonstrated that the RMs with a BSA:KC ratio of 1:2 have a typical cubic structure (0.93 μm) and excellent gel strength. RMs adsorbed at the oil–water interface to form non-covalent binding layers, which improved interfacial activity and reduced interfacial tension (95.10°) to obtain more uniform and stable Pickering emulsions. The above results indicate that synthesizing RMs provides a feasible method for constructing uniform and stable Pickering emulsions with low oil content, these are similar to high internal phase emulsions with semi-solid rigid structures.

Experimental study on bond performance of recycled coarse aggregates concrete with circular steel tubes after freeze–thaw cycling

AbstractExtrusion tests were conducted on recycled coarse aggregate concrete with circular steel tubes (RCCST, 100% replacement of recycled coarse aggregate) to investigate the impact of freeze–thaw cycles on bond properties. RCCST samples were subjected to 0, 25, 50, 75, 100, 125 and 150 freeze–thaw cycles and their bond properties were then compared with those of natural coarse aggregate concrete with circular steel tubes (NCCST) after 0, 100 and 150 freeze–thaw cycles. The experimental results indicate that freeze–thaw cycling damages the internal structure of RCCST, causing a significant decrease in interfacial bond strength with an increase in the number of freeze–thaw cycles. Freeze–thaw RCCST exhibits lower peak load and higher peak slip compared to NCCST, with increases of 25.2%, 73.2%, and 70.3% after 0, 100, and 150 freeze–thaw cycles, respectively. Regression analysis was used to derive an exponential equation that describes the relationship between longitudinal strain and strain gauge position. Additionally, a segmented fitting method was employed to obtain an expression for the bond slip of the freeze–thaw RCCST with a circular steel pipe.

Macro–micro correlation analysis on the loess from Ili River Valley subjected to freeze–thaw cycles

The Ili River Valley in Xinjiang, China, is a typical seasonal frozen area where loess landslide disasters have become increasingly common during the freeze–thaw periods in recent years. This study analyzed the macroscopic mechanical strength and microstructure changes of the Ili loess under different freeze–thaw cycles (FTCs) through the post-freeze–thaw triaxial compression test on the unsaturated soil in laboratory. Apart from the scanning electron microscopy (SEM), and the nuclear magnetic resonance (NMR), the macro–micro correlation analysis and the cluster-principal component analysis were applied for the theoretical discussion. The results indicated that the cohesive force of the loess exhibits an initial decreases, followed by the increases, and eventually keep stable after various FTCs, while the internal friction angle showed the opposite developing trend before the final constant. Similar to the strong correlation between the cohesive force and the particle abundance, the internal friction angle is also closely related to the abundance and orientation fractal dimension of the loess particles. However, the principal component analysis results showed that cohesive force strongly correlates with the average maximum pore size and the pore size fractal dimension, for which the internal friction angle most strongly affected by the average maximum particle size. The possible reason is that the extracted principal components represent a class of microscopic parameters with the same or similar change trend, although there may be a certain offset between them. The mechanical deterioration of loess is attributed to the repeated frost heaving force and the migration potential caused by FTCs. The alterations of the microstructure accelerated the deterioration of the macroscopic mechanical properties of the loess, which further widens the understanding of the mechanism behind the deterioration of loess mechanical strength in the Ili River Valley under FTCs, and contributes to the prevention and management of the local landslide disasters.

Electrical characterization of freeze-thaw damage in saturated concrete and its interfacial transition zones

Determining the freeze-thaw (F-T) damage of the interfacial transition zone (ITZ) is crucial to understanding the mechanism of F-T in concrete and improving its frost resistance. In this paper, the F-T damage of saturated concrete/mortar and ITZ is characterized using AC impedance spectroscopy, and the micro-mechanisms of F-T damage in cement-based materials (CBMs) are summarized. Firstly, the damage factor was defined by the effective resistivity of the CBMs, and a correlation between the damage factor and the compressive strength loss was established. The F-T damage process was divided into a damage development stage and a damage stabilization stage based on the damage factor and fractal dimension. Then, the effective resistivity separation formula was proposed to calculate the ITZ effective resistivity, and the ITZ damage factor was calculated for non-destructive quantitative characterization of the F-T damage in the ITZ. Finally, the microscopic mechanism of F-T damage in CBMs and their ITZs was summarized based on damage factor, fractal dimension, super depth of field microscopy, and nanoindentation. The results showed a linear relationship between the damage factor and compressive strength loss (Lc = 0.176Er+0.030); the ITZ had damage factors of 80 % and 70 %, which were 1.6 and 1.4 times higher than those of the matrix, respectively; F-T cycles produce micro-damage, part of which connects with the disconnected pores to form connected pores, the micro-damage extends and accumulates along the surface of the aggregates, leading to ITZ debonding from aggregate and deterioration of the mechanical properties.

Study on physical and mechanical properties of high-grade highway subgrade slate in permafrost region under freeze–thaw cycles

The subgrade crushed-rocks of Gonghe-Yushu (Gongyu) Expressway in Qinghai Province are seriously weathered, resulting in a series of pavement diseases. Among the weathered crushed-rocks, the weathering degree of slate is particularly serious, and its physical and mechanical properties, weathering resistance and applicability are not clear. Therefore, this paper takes the slate in the subgrade crushed-rocks of Gongyu Expressway as the research object, and drills the core of the slate rock block to make a cylindrical standard sample, and uniaxial and triaxial compression tests, nuclear magnetic resonance tests, and electron probe micro-analysis tests were performed on it within 50 freeze–thaw cycles (FTC) under saturated conditions. According to the test results, the mass, longitudinal wave velocity, and strength of the slate specimens all decrease with the increase of the number of FTC, the cohesion (C)\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$({\ ext{C}})$$\\end{document} increases first and then decreases, and the change trend of internal friction angle (φ) is completely opposite to the cohesion. The FTC has an expansion effect on the pores of the slate specimens, and the microstructure of the rock particles on the specimen’s surface is removed and becomes smooth. The results of mechanical tests are used in the Hoek–Brown (H-B) strength criterion, and a unified expression of the H-B criterion suitable for slate in permafrost regions is established. The above conclusions can provide some construction reference and maintenance of high-grade highways in cold regions.

Effect of Cyclic Soil Freezing and Thawing on the Lateral Load Response of Bridge Pile Foundations

In this article, a nonlinear static analysis model of a bridge pile foundation is established using numerical simulation, and the correctness of the model is verified via experiments. Then, the damage characteristics and mechanical behaviors of bridge pile foundations in cold regions under lateral loads are investigated based on the validated analysis model. The results showed that the impact of soil freeze–thaw cycles on the lateral performance of the pile–soil system is more pronounced in seasonally frozen regions compared with permafrost regions. Specifically, as the number of soil freeze–thaw cycles increases, there is a tendency for the lateral load capacity of the pile–soil system to decrease initially and then stabilize. It is worth noting that soil freeze–thaw cycles significantly influence both the stiffness and deformation capacity of the pile–soil system, with these parameters exhibiting a decreasing trend followed by stabilization as the number of freeze–thaw cycles increases. However, it has little effect on the shear force and bending moment of the pile foundation.

Research on Solidification Methods and Stabilization Mechanisms of Sulfate Saline Soils

In cold regions, saline soils can cause dissolution, settlement, and salt expansion of the roadbed under the influence of freeze–thaw cycles, so they need to be stabilized during road construction. In this study, lime, fly ash (FA), and polyacrylamide (PAM) were used to stabilize sulfate saline soils, and the stabilized saline soils were subjected to the unconfined compressive strength test (UCS), splitting test, and freeze–thaw cycle tests (FTs). The stabilization mechanism of the three materials on saline soils was also studied via scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG), and X-ray photoelectron spectroscopy (XPS). The test results showed that the addition of lime, FA, and PAM to saline soils can improve the mechanical properties and frost resistance of saline soils. After 28 d of curing, the UCS of FA-, PAM-, and lime-stabilized saline soils increased by at least 55%, 23%, and 1068%, respectively, and the splitting strength increased by at least 161%, 75%, and 2720%, respectively. After five freeze–thaw cycles, the residual strength ratios (BDRs) of the UCS of L2 (lime 8%), F2 (FA 11%), and P2 (PAM 1%) stabilized soils and saline soils were 71.78%, 56.42%, 39.05%, and 17.95%, respectively, and the decreasing trend tended to be stable. The saline soils stabilized by lime and FA were chemically stabilized, and their mechanical properties and frost resistance were better than the physical stabilization of PAM.

Using established biorepositories for emerging research questions: a feasibility study

BackgroundProteomics and metabolomics offer substantial potential for advancing kidney transplant research by providing versatile opportunities for gaining insights into the biomolecular processes occurring in donors, recipients, and grafts. To achieve this, adequate quality and numbers of biological samples are required. Whilst access to donor samples is facilitated by initiatives such as the QUOD biobank, an adequately powered biobank allowing exploration of recipient-related aspects in long-term transplant outcomes is missing. Rich, yet unverified resources of recipient material are the serum repositories present in the immunological laboratories of kidney transplant centers that prospectively collect recipient sera for immunological monitoring. However, it is yet unsure whether these samples are also suitable for -omics applications, since such clinical samples are collected and stored by individual centers using non-uniform protocols and undergo an undocumented number of freeze–thaw cycles. Whilst these handling and storage aspects may affect individual proteins and metabolites, it was reasoned that incidental handling/storage artifacts will have a limited effect on a theoretical network (pathway) analysis. To test the potential of such long-term stored clinical serum samples for pathway profiling, we submitted these samples to discovery proteomics and metabolomics.MethodsA mass spectrometry-based shotgun discovery approach was used to obtain an overview of proteins and metabolites in clinical serum samples from the immunological laboratories of the Dutch PROCARE consortium. Parallel analyses were performed with material from the strictly protocolized QUOD biobank.ResultsFollowing metabolomics, more than 800 compounds could be identified in both sample groups, of which 163 endogenous metabolites were found in samples from both biorepositories. Proteomics yielded more than 600 proteins in both groups. Despite the higher prevalence of fragments in the clinical, non-uniformly collected samples compared to the biobanked ones (42.5% vs 26.5% of their proteomes, respectively), these fragments could still be connected to their parent proteins. Next, the proteomic and metabolomic profiles were successfully mapped onto theoretical pathways through integrated pathway analysis, which showed significant enrichment of 79 pathways.ConclusionsThis feasibility study demonstrated that long-term stored serum samples from clinical biorepositories can be used for qualitative proteomic and metabolomic pathway analysis, a notion with far-reaching implications for all biomedical, long-term outcome-dependent research questions and studies focusing on rare events.

Salt frost resistance of concrete with crushed asphalt particles: Experiments and modelling

Asphalt coating is widely utilized for its excellent impermeability on marine structure surface. However, in high latitude regions, the coupling effect of freeze-thaw cycles and chemical aging rapidly diminished the protective effect, significantly increasing the risk of structural damage. Therefore, to maximize the impermeability and durability of asphalt, we homogeneously intermixed the crushed asphalt particles into the concrete instead of surface coating. A series of crushed asphalt particles concrete (CAPC) with different asphalt content were prepared and subjected to up to 30 salt freeze thaw cycles (SFTCs) in a 3.5 wt% NaCl solution. Experimental results revealed that addition of asphalt particles improved pore structure and impermeability, mitigating frost damage and chloride diffusion. Optimal performance is observed at 2 % asphalt content, showcasing a notable 22.0 % reduction in porosity, a substantial 47.3 % decrease in chloride concentration at 10 mm depth after 30 SFTCs, with only a 3 % slight reduction in strength. Moreover, a multi-phase chloride diffusion model was proposed, considering matrix/asphalt damage and time-varying porosity. The influence of aggregate shape and volume, data-processing methods, long-term prediction under different regions were discussed. Compared with asphalt coating and classical concrete, the proposed CAPC is recommended for use under marine environment, especially in cold marine regions.

Impact of freeze–thaw cycling on the stability and turnover of black soil aggregates

During freeze–thaw cycling, aggregates undergo a dynamic change in breakdown–formation (turnover), however, how the turnover occurs between aggregates of various particle sizes is not clear. To clarify the influence of freeze–thaw cycling on the dynamic changes in the particle size of soil aggregates, soil aggregates from the Black Soil Region of Northeast China were selected as the research objects. The study conducted in situ dynamic monitoring experiments, innovatively applying the rare earth oxide (REO) tracer method to natural conditions of freeze–thaw cycles (autumn freeze–thaw period, freezing period, and spring freeze–thaw period), accurately tracking the turnover paths and quantifying the turnover rates between aggregates of various particle sizes. The results revealed that the total value of the formation paths of the 2–5 mm aggregates and 0.25–2 mm aggregates increased during the autumn freeze–thaw period. The number of freeze–thaw cycles and accumulated snowfall were significantly positively correlated with aggregate stability, with an increase in the number of freeze–thaw cycles and accumulated snowfall resulting in an increase in the proportion of aggregates > 0.25 mm, which improved aggregate stability. In addition, the total value of the breakdown path of macro-aggregates increased during the spring freeze–thaw cycling period. Soil moisture was significantly negatively correlated with aggregate stability, with increased soil moisture resulting in a decrease in the percentage of aggregates > 0.25 mm, which resulted in a decrease in aggregate stability. The study can provide a reference understanding for the effects of freeze–thaw cycles on the structure of black soil and provide a theoretical basis for improving the quality of arable land.

Heat transfer deformation test and model of coal during LN2 cyclic freezing and thawing process

Exploring the temperature response and deformation characteristics of coal during the freeze–thaw process is crucial for maintaining coal seam stability under liquid nitrogen (LN2) treatment and enhancing gas extraction efficiency. This study investigates the temperature diffusion and deformation characteristics of coal during LN2 cyclic freeze–thaw processes, using saturated and dry anthracite samples. Real-time monitoring of the freeze–thaw characteristics of the anthracite samples was conducted using temperature and strain detectors to quantitatively analyze the evolution of coal temperature and strain. Additionally, a nuclear magnetic resonance (NMR) testing system was used to monitor the pore evolution characteristics of the coal before and after LN2 treatment. The results indicate: After LN2 cyclic freeze–thaw, the internal pores of the saturated samples were highly developed, and fracture connectivity was enhanced, whereas the pores in the dry samples significantly decreased, leading to a sharp reduction in overall connectivity. The cooling and heating rates of the saturated samples were higher than those of the dry samples, both exhibiting exponential trends. This variation is primarily due to the phase change of pore water and the formation and expansion of the internal fracture network within the coal. The deformation magnitude of both sample types showed an overall exponential decline, with the saturated samples exhibiting higher deformation magnitudes than the dry samples. The presence of fracture water not only accelerates coal deterioration during the freeze–thaw process but also enhances coal cementation, maintaining deformation stability. Furthermore, this paper derives a temperature-strain coupling model for coal subjected to LN2 F-T cycles, aiming to provide a reference for understanding the freeze–thaw damage mechanism of coal and the practical application of LN2 fracturing.

Insight into the crack evolution and mechanism of catalyst-coated membrane undergoing freeze–thaw cycling in fuel cells

The evolution of catalyst-coated membrane (CCM) under saturated humidity conditions during freeze–thaw cycling was investigated, with a particular focus on the evolution of initial cracks within the catalyst layer (CL) and their impact on the proton exchange membrane (PEM). A quasi in-situ fuel cell fixture was designed to enable direct observation of the CCM via scanning electron microscopy (SEM). As the number of freeze–thaw cycles increases, the initial cracks in the CL gradually propagated, with new cracks appearing around them. These CL cracks tend to extend along the larger pores, which essentially represent gaps between agglomerates. The overall trajectory of formed cracks appears to be influenced by the flow channel direction. Additionally, under freeze–thaw cycling, cracks in the CL led to the formation of cracks in the PEM. A strong correlation is found between the direction of membrane cracks and CL cracks, suggesting a direct impact of the CL defects on the structural integrity of the PEM. Furthermore, some cracks at the tips of CL cracks even penetrate through the entire membrane. This study provides valuable insights into the degradation mechanisms of CCMs under low-temperature environmental conditions and could inform future design improvements for enhanced durability.

Development and characteristic research on new thermal insulation and anti-permeability grouting material for tunnels in cold regions

The surrounding rock of tunnels in cold regions are susceptible to the freeze–thaw cycle resulting from the combination of low temperatures and moisture during tunnel service. The phenomenon will not only lead to the expansion of pores and fissures in the surrounding rock of the initial tunnel, but also destroy the integrity of the rock. This destruction will have a serious impact on tunnel structure and rail transit operation safety. At present, the commonly used thermal insulation measures have some problems such as maintenance difficulties, low economic efficiency, and safety hazards. Therefore, it is urgent to develop a kind of tunnel maintenance grouting material with insulation and anti-permeability, which has the characteristics of simple operation, easy preparation and application. We independently developed a composite grouting material composed of polyurethane (PU), epoxy resin (E-51) and acrylic powder (PMMA). Through the material combustion test, magnesium hydroxide was selected as the flame retardant additive. Moreover, we measured the thermal conductivity, water absorption, apparent density, porosity and strength characteristic parameters. The thermal insulation and anti-permeability characteristics of the composites were also analyzed. The results indicated that the thermal conductivity of the new composite grouting material is 6.3% lower than the PU before adding flame retardant. Compared with the PU with flame retardant, the water absorption decreased by 74.4% and the ultimate strength increased by 33.3%. For the area with an average temperature lower than − 10 °C, we recommend the ratio scheme of E-51: 3%; PMMA: 15%. For the area with high water content in the surrounding rock of the tunnel, we recommend the ratio scheme of E-51: 15%; PMMA: 3%. This study provides new ideas for material preparation and tunnel insulation methods for anti-freezing measures in tunnels during their operational period in cold regions.

Sustainable and Robust Cellulose‐Based Core–Shell Hydrogels Recycled from Waste Cotton Fabrics as High‐Performance Food Coolants

AbstractIdeal temperature condition is one of the essential determinants that critically impact the quality of food products. Conventional water‐based ice cubes present challenges from meltwater being breeding grounds for microorganisms and heightening the risk for cross‐contamination. Hereby, the presented cellulose‐based hydrogels crosslinked by epichlorohydrin are dip‐coated with alginate/calcium chloride to form a core–shell structure for achieving the critical benchmarks of an ideal food coolant with limited meltwater production, high‐water retention capacity, and high mechanical strength. The structures and properties of the hydrogels before and after freeze–thaw cycles are characterized by scanning electron microscopy, compressive test, water retention test, and differential scanning calorimetry. All formulated hydrogels demonstrate promising compressive strength, latent heat of fusion, and water retention properties. Notably, the C2A10Cl hydrogel exhibits a maximum compressive strength of 144.7 kPa and high latent heat of fusion of 272.5 J g–1, which is better than previously reported sustainable hydrogel coolants. Furthermore, comparison studies reveal that the cellulose‐based hydrogels demonstrate a similar thawing pattern to conventional ice cubes but without the generation of any meltwater. The temperature of blueberries can be cooled down from 22 to 3.9 °C in 32 min by the hydrogels and in 26 min by ice cubes, respectively.

Transient Adsorption of Zwitterionic Fluoroquinolones on Goethite During Freeze–Thaw Cycles

Transient Adsorption of Zwitterionic Fluoroquinolones on Goethite During Freeze–Thaw Cycles

Effect of freeze-thaw treatment on the yield and quality of tiger nut oil

To investigate the effect of freeze-thaw (FT) process on the yield and quality of tiger nut oil, tiger nuts were subjected to 0–12 cycles of FT treatment. Results indicated that FT treatment ruptured the cell structure of tiger nut, resulting in an increase in oil yield. Acid value (2.09–2.42 mg KOH/g) and peroxide value (0.40–0.42 mmol/kg) increased with the number of FT cycles, but the increments were small. Likewise, slight differences in fatty acid composition and thermal properties between control and FT-treated samples were observed. FT treatment remarkably increased the bioactive components (e.g., vitamin E, sterols, chlorophyll and carotenoids) in the oil and extended the oxidation induction time from 1.2 to 5.57 h. FT treatment altered the volatile composition of tiger nut oil, increasing the relative content of heterocycles and pyrazines such as 2-methoxy-4-vinylphenol, trimethylpyrazine and tetramethylpyrazine. It was suggested that FT treatment prior to oil extraction was beneficial to improve the oil yield and quality.

Frost Resistance Differences of Concrete in Frequent Natural Freeze–Thaw versus Standard Rapid Method

In order to find the anti-freezing durability differences between concrete in the frequent natural freeze–thaw conditions in the northwest of Sichuan Province, China, and concrete in the rapid freeze–thaw conditions of the standard rapid method, the typical temperature and humidity of the northwest of Sichuan Province were simulated. The results showed that the average number of freeze–thaw cycles in the northwest of this province can reach up to 150 per year. The relative dynamic modulus of C30 ordinary concrete, which is 100% pre-saturated, still remained above 90% after 450 cycles in simulated environments. However, during the rapid freeze–thaw test, even the C30 air-entrained concrete failed after 425 cycles. Compared to the saturation degree of concrete itself, the continuous replenishment of external moisture during freeze–thaw cycles is a key factor affecting the frost resistance of concrete. Rapid freeze–thaw reduces the number of the most probable pore sizes in ordinary concrete, and the pore size distribution curve tends to flatten. The reduction rate of the surface porosity of air-entrained concrete before and after rapid freeze–thaw is only about one third of that of ordinary concrete.