Additive manufacturing methods, particularly 3D printing, are widely utilized in research and engineering for crafting lightweight yet durable materials capable of withstanding substantial forces. Leveraging insights from biomechanical structures offers a deeper understanding of reinforcement techniques and optimal design strategies to enhance strength. This paper focuses on the development of a Draisine design, historically significant in Karlsruhe, as part of the BWSplus project "Drais3D-Trinational" workshop. Through a comprehensive analysis of bionic influences on lightweight design and 3D printing parameters, this study aims to create an optimal design framework for the workshop. Utilizing Computer-Aided Design (CAD) software such as SolidWorks and Creo Parametric, along with Finite Element Analysis using ANSYS R2023 Workbench, deformation and stress analysis are conducted. Investigation into 3D printing parameters, including infill patterns, temperature, support systems, and orientation, seeks to identify optimal solutions considering factors like processing time, robustness, and filament wastage. The objective is to explore the biomechanical influence on construction methods, concept design, and parameter construction in preparation for the Drais3D-Trinational workshop in March 2023. The expected outcomes include the presentation of two main designs with varied parameters, alongside analyses such as Finite Element Analysis and optimization of 3D printing parameters, emphasizing the role of bionic structures in defining the optimal Draisine design.