Formulation F2 Research Articles

Formulation and evaluation of aceclofenac fast disintegrating tablets using sida acuta powder

Aceclofenac is a NSAID with anti-inflammatory, analgesic activity. The aim is to develop the Aceclofenac tablets with sida acuta powder that exhibit rapid disintegration, dissolution and showing maximum pharmacological activity in less time. This is desirable for improving patient compliance. The present study was to formulate and evaluate the fast-disintegrating tablets of Aceclofenac using Sida acuta powder by direct compression technique with varying concentrations. Each formulation was evaluated for pre and post evaluation tests such as Flow property, Bulk density, tapped density, Weight variation, Hardness, Friability, Wetting time, Disintegration time, Assay, in-vitro dissolution. Among the 06 formulations, F6 formulation Aceclofenac with 6%w/w of Sida acuta showed the in-vitro dissolution (99.75%) release of drug within 20 minutes and release of drug mechanism as first order kinetics. It was concluded that the fast-disintegrating tablets are prepared by Natural polymer Sida acuta acts as a disintegrant and showed excellent disintegration time,enhance the dissolution rate.

Multifunctional hydrogel based on silk fibroin/thermosensitive polymers supporting implant biomaterials in osteomyelitis

Abstract The search for novel antibiotic-loaded biomaterials that support the management of osteomyelitis has been extensive in current years. This study developed multifunctional hydrogel to enhance the performance of osteo-conduction and antimicrobial as supporting implant materials for osteomyelitis treatment and prevention. Ciprofloxacin-loaded thermosensitive hydrogels composed of silk fibroin and poloxamers were prepared using the cold method. F1, F2, and F3 formulations resulted in gelation time of within 3 min under 37°C. Physical evaluation showed that the formulations, especially F3, had a proper swelling ratio and 82.34% biodegradability within 14 days. Ciprofloxacin from formulations exhibited an initial burst release within 24 h and continuous delivery, with more than 84% release over 14 days. Released antibiotics showed efficacy against Staphylococcus aureus ATCC25923 and eradicating activity against biofilms. Moreover, hydrogels had no cytotoxic effect on MC3T3-E1 cells with 93–106% cell viability. This multifunctional hydrogel showed a promising approach for supporting osteomyelitis treatment and prevention.

Clinical Investigation of Valsartan Sustained-Release Matrix Tablets: Formulation Design and Performance Evaluation

The primary objective of this main study is to develop and assess the sustained-release matrix tablets containing Valsartan, an angiotensin II receptor type 1 antagonist. The powder mixtures underwent a thorough examination of pre-compression parameters and observed angle of repose, bulk density, tapped density, and Carr’s index, all of which exhibited satisfactory results. Following compression, the tablets were subjected to post-compression evaluations, including weight variation, thickness, hardness, friability, drug content, in-vitro dissolution, and stability studies. In-vitro dissolution investigations are conducted over 24 hours, employing 0.1 N HCL for the initial 2 hours and pH 6.8 phosphate buffer for the subsequent 24 hours. Notably, formulations F4 and F7 demonstrated promising dissolution profiles, effectively controlling the release of the drug. These formulations, enriched with higher concentrations of chitosan and sodium alginate in addition to other polymers, successfully sustained the drug release for the entire 24-hour duration. The compatibility of the drug, polymers, and other excipients was meticulously assessed using FT-IR Spectroscopy, affirming the harmonious interaction among these components. Further analysis involved fitting the release data to various mathematical models, including Zero-order, First-order, Higuchi equation, and Korsmeyer-Peppas model, to ascertain the kinetics and mechanisms governing drug release. Results indicated that the drug release adhered to first-order kinetics, with a non-Fickian mechanism prevailing. Stability studies conducted for 3 months provided reassurance regarding the stability of the selected formulations (F4 and F7), bolstering confidence in their potential for sustained release of Valsartan.

Preparation and Evaluation of Sustained Release Matrix Tablets of Acelofenac and Comparision of Formulated and Marketed Product

The aim of performing this study is to develop matrix tablet to improve the dissolution rate of aceclofenac and release the drug in a controlled manner over a period of 24 hours. Matrix tablets of aceclofenac, using various viscosity of hydrophilic polymer HPMC in two different proportions, hydrophobic polymer ethyl cellulose and Guar gum were prepared by wet granulation method and subjected to in vitro drug release studies. Tablets were evaluated for in vitro drug release profile in phosphate buffer with pH 7.5. The thickness and hardness of prepared tablets were 3.8 ± 0.2 to 3.9 ±0.2 mm and 4 ± 3 to 5 ± 3 kg/cm2, respectively. The friability was within the acceptable limits of pharmacopeial specifications (0.31 to 0.71%), which indicates the good mechanical strength of the tablets. The in vitro drug release from the proposed system was best explained by Higuchi’s model, indicating that drug release from tablets displayed a diffusion-controlled mechanism. Based on the study results, formulation F7 was selected as the best formulation.

Development and Characterization of Multi-Nutrient Formulations for Organic Farming

The study was to develop multi-nutrient formulations for organic farming containing all the essential macro and micronutrients. The experimental design selected was completely randomized design with 8 treatments and 3 replications. The experiment was conducted in the Department of Soil Science and Agricultural Chemistry, College of Agriculture, Vellayani, Kerala between Jan 2021-May 2021. Multi-nutrient formulations were developed using various organic nutrient sources like blood meal, soybean meal, rock phosphate, steamed bone meal, potassium sulfate, langbeinite, epsom salt, and borax permitted by National Programme for Organic Production. Formulations were prepared by mixing nutrient sources considering the nutrient requirement of nendran banana (N:P2O5:K2O @ 300:115:450 g plant-1) and the soil fertility status. The formulations were characterized for their physical, chemical and biochemical properties. On the basis of character analysis and principal component analysis the formulation F1 containing blood meal, rock phosphate, potassium sulfate, epsom salt and borax was found superior to other formulations. This was followed by F3 which contains blood meal, steamed bone meal, potassium sulfate, epsom salt and borax. Formulation F1 had 3.73 g cm-3 bulk density, 2.67% moisture content, 6.5 pH, 3.23 dSm-1 EC, 29.43% OC, 7.21% N, 2.71% P, 10.78% K, 5.98% Ca, 0.35% Mg, 4.45% S, 1174.11 mg kg-1 Fe, 4.53 mg kg-1 Mn, 13.55 mg kg-1 Zn, 8.65 mg kg-1 Cu and 93.67 mg kg-1 B. It also contained 45.04% crude protein, 2.95% humic acid and 3.55% fulvic acid. The formulation F3 had 7.47 pH, 3.37 dSm-1 EC, 28.34% OC, 7.11% N, 2.68% P, 10.68% K, 2.05% Ca, 0.32% Mg, 4.37% S, 1163.41 mg kg-1 Fe, 4.87 mg kg-1 Mn, 14.52 mg kg-1 Zn, 9.05 mg kg-1 Cu and 96.33 mg kg-1 B. Hence multi-nutrient formulation F1 based on its nutrient supplying capacity can be recommended as a nutrient source for organic farming.

Formulation and Evaluation of Ketoprofen Emulgel for Topical Drug Delivery System

Emulgel have emerged as an auspicious drug delivery system for the delivery of hydrophobic drugs. The impartial of the study was to prepare emulgel of ketoprofen, a non-steroidal anti-inflammatory drug, using Carbopol 940 as a gelling agent. Mentha oil and clove oil were used as diffusion enhancers. The emulsion was prepared and it was combined in a gel base. The formulations were evaluated for spreading coefficient studies, rheological studies, and ex vivo release studies. Formulations F2 and F3 show maximum release. So, it can be concluded that topical emulgel of ketoprofen possesses an effective anti-inflammatory and analgesic activity.

Study and characterization of a product based on a vegetable extract of quinoa fermented with water kefir grains.

This work aimed to study and characterize a product based on vegetable extract of quinoa (WVEQ) fermented with water kefir grains. The effect of sucrose concentration (SC), inulin concentration (IC), and xanthan gum (XG) concentration were evaluated using a central composite design (CCD) 23. They were subsequently characterized regarding cellular growth of the grains, beverage yield, pH, soluble solids, carbon dioxide (CO2) production, lactic acid, and ethanol production. Therefore, for the final stage, two formulations (F1 and F8) of the CCD were chosen to be characterized in terms of proximate composition, microbiological composition of the kefir culture, analysis of organic compounds, sensory analysis, and enzymatic and microbiological characterization before and after simulation of in vitro gastrointestinal digestion. In the two chosen products, one can see that fermentation optimized the bioavailability of proteins due to the high proteolytic activity of the microorganisms in kefir and the increase in lipid content. In identifying microorganisms, there was a prevalence of Saccharomyces sp. yeasts. In the sensory analysis, the F8 formulation showed better results than the F1 formulation. In vitro, gastrointestinal digestion showed reduced lactic acid bacteria and yeast and increased acetic acid bacteria in the liquid phase for both formulations. In the enzymatic profile, there was a reduction in all enzymes analyzed for both formulations, except for amylase in F1, which went from 14.05 U/mL to 39.41 U/mL. Therefore, it is concluded that using WVEQ as a substrate for the product appears to be a viable alternative with nutritional and technological advantages for serving a specific market niche.

Design, Development and In-Vitro Characterization of Insulin Loaded Topical Pluronic-Lecithin Based Organogel Formulation for the Management of Diabetic Wound.

To develop and characterize the topical insulin-loaded organogel formulation for the management of diabetic wounds. To formulate and evaluate organogel of insulin that can serve as a topical administration for promoting enhanced wound healing in diabetic patients by providing sustained and localized delivery of drug to the wound site. The insulin organogel formulated by the micro-emulsion method involves mixing the "aqueous and oil phases" at high shear. Physical and chemical properties, as well as an in vitro study with a Franz diffusion chamber, were used to evaluate the prepared organogel. All formulations proved to be off-white, homogeneous, washable, and had a pH between 6 and 6.5; moreover, they were non-irritating and skin-compatible. Formulations F1-F6 had viscosity ranging from 2058 to 3168 cps, spreadability ranges of 0.35 to 0.52 g*cm/s, and gel transition ranges of 28.33 to 35.33 °C. In formulations F1-F3, the concentration of lecithin was gradually increased, and in formulations F4-F6, the concentration of PF-127 was increased, resulting in a decrease in gel transition temperature, an increase in viscosity, and a gradual change in spreadability. The higher-viscosity formulations were much more stable and had better drug release. All formulations were fitted to a kinetic model belonging to first-order kinetics. However, after examining the parameter evaluation, it was found that the formulations F2 and F6 were better suited to the kinetic model and were consistent with the first-order and Higuchi models in Korsmeyer-Peppas F2 (r2 = 0.9544 and n = 1.0412); F6 (r2 = 0.9019 and n = 1.0822), which was a confirmation of the sustainability of the release system with matrix diffusion and drug delivery mechanisms that were based on the Super-Case II transport. Further research and clinical trials are needed to validate its efficacy, optimize the formulation, and establish its long-term safety. Topical insulin organogel has the potential to revolutionize diabetic wound management by improving healing outcomes, reducing complications, and raising the standard of living for those who have diabetes.

Development and In vitro Assessment of Topical Microemulsion Based Pluronic-Lecithin Organogel for the Management of Arthritic Pain

Background: Topical delivery of NSAIDs through organogels might transport lornoxicam to the site of action, minimizing gastrointestinal problems and adverse effects. Methods: In the current investigation, a lecithin organogel containing lornoxicam was made by microemulsion method. For this purpose, a certain amount of pure soya lecithin was dispersed in suitable isopropyl myristate as a dispersant and emulsifier at room temperature to form the oily phase. The lecithin was completely dissolved in the combination by the next morning. Sorbic acid was then added to the mixture as a preservative, Pluronic F-127 and potassium sorbate were weighed out, and then they were mixed with cold water to create an aqueous phase, and menthol was added. On the next morning, lornoxicam, the active component, became soluble in polyethylene glycol-400 and combined with the lecithin isopropyl palmitate mixture. The oily portion was agitated using a mechanical stirrer at 400 rpm while the aqueous phase was introduced gradually. Results: The lornoxicam organogel preparation was it was assessed for its physical appearance, organoleptic characteristics, consistency, gelation temperature, drug content, and in vitro release studies. The active ingredient content of formulation F5 was the highest at 93.33. Formulations F4 and F5 were selected for kinetic studies because they had all physical characteristics under reasonable limits, the active ingredient level was the greatest, and the active ingredient release was the fastest in eight hours. Conclusion: The transdermal organogel formulation of lornoxicam was found to be effective for topical distribution of the drug and when administered topically, it has strong anti-inflammatory and anti-rheumatic action.

Preparation of herbal hair oil exploring the therapeutic benefits of herbs and its evaluation

Background: Herbal cosmetics, particularly herbal hair oils, have gained popularity due to their perceived efficacy and minimal adverse effects. This study aimed to formulate and evaluate herbal hair oils for promoting hair growth. Methodology: Various herbal extracts, including Coconut, Curry leaves, Amla, Fenugreek, and Onion, were prepared and incorporated into virgin Coconut oil. Phytochemical screening, total flavonoid content, organoleptic evaluation, chemical analysis (acid value, saponification value, specific gravity), stability studies, antioxidant assay (DPPH), antimicrobial assay, sensitivity studies, and in-vivo hair growth activity were conducted. Results: Organoleptic evaluation revealed characteristic odors and colors across formulations. Chemical analysis showed acceptable values for acid value (0.68 - 1.86 mg KOH/g), saponification value (117.87 - 224.27 mg KOH/g), and specific gravity (0.865 - 0.933 g/cm^3). Stability studies over 45 days demonstrated consistent physicochemical properties. The DPPH assay indicated dose-dependent antioxidant activity, with inhibition ranging from 13.8% to 66.5%. Antimicrobial assay showed inhibition zones ranging from 6 mm to 20 mm against Staphylococcus aureus. Sensitivity studies exhibited no adverse reactions. In-vivo hair growth activity demonstrated significant improvements compared to controls. Discussion: The formulated herbal hair oils exhibited promising physicochemical properties, antioxidant activity, antimicrobial efficacy, safety, and hair growth promotion in animal models. These findings suggest their potential as natural remedies for hair care. Conclusion: Herbal hair oils formulated in this study show significant potential for promoting hair growth and addressing hair-related concerns. Whereas, formulation F3 shown significant efficacy across multiple parameters among the formulations. Further research and clinical trials are warranted to validate their efficacy and safety for human use.

FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLET OF PROCHLORPERAZINE MALEATE

In the present work, fast disintegrating tablets of prochlorperazine maleate were designed with a view to enhance patient compliance by effervescent method. Prochlorperazine Maleate, a potent antipsychotic medication with antiemetic properties, holds significance in treating various conditions such as nausea, vomiting, and vertigo. The decision to formulate it into orodispersible tablets aimed to address issues related to patient compliance and convenience, particularly for individuals who experience difficulty swallowing conventional tablets. Formulation F10, enriched with 10% Croscarmellose sodium as a superdisintegrant and an effervescent mixture for taste masking, exhibited remarkable characteristics. Croscarmellose sodium, chosen for its exceptional disintegration properties, ensures rapid breakdown upon contact with saliva, facilitating swift drug release and subsequent onset of action. Moreover, the incorporation of an effervescent mixture not only aids in masking the potentially unpleasant taste of Prochlorperazine Maleate but also holds the potential to enhance palatability and overall patient acceptance. Physicochemical characterization of Prochlorperazine Maleate unveiled favorable attributes, including a loss on drying of 0.413±0.002%, a melting point range of 234-236°C, and a moisture content of 0.0354%, affirming its suitability for formulation into orodispersible tablets. Through dissolution profile comparisons, formulation F10 demonstrated superior drug release kinetics compared to the commercial formulation, underscoring its potential therapeutic advantages.

Optimization of an efficient hydroponic cultivation method for high yield of strawberry plants

The widely grown octoploid strawberry (2n = 8x = 56) (Fragaria × Ananassa Duch.), cherished for its delectable flavour, rich nutritional content, and pleasing aroma has consistently held a significant position in global fruit market. Despite of rising demand, strawberry plants are not grown universally, likely due to variations in weather conditions across regions, available of infectious soil pathogen and insufficient knowledge of soilless hydroponic cultivation. The current research aims to establish an efficient nutrient medium that enhances the growth and yield of strawberry (Fragaria × Ananassa Duch.) Cv. Winter Dawn, using soilless hydroponic and semi-hydroponic cultivation methods. The fruit fresh weight and total fruit yield of formulation 2 treated plants (EC 2.41 mScm−1) in hydroponic condition exhibited a 1.73-fold and 2.18-fold higher respectively as compared to soil control plants. Among the five different growing medium treatments in semi-hydroponic condition, the fresh weight and total strawberry fruit yield was found to be maximum in treatment 3 and observed 2.04-fold and 3.03-fold increase respectively as compared to plants grown in soil control condition. The high yield may be due to higher uptake of macronutrients (NPK) by the plants supplemented with F2 formulation and T3 substrate medium the plants. As a result, the strawberry fruits of these plants accumulated higher amount of anthocyanin, which ultimately enhanced the fruit quality. The studies concluded that formulation 2 in hydroponic and treatment 3 in semi-hydroponic were most effective in terms of growth, nutrient uptake, physiology, fruit yield and anthocyanin content. The result of this study gives an alternative to the farmers for cultivation of strawberry plants in soilless hydroponic and semi-hydroponic conditions.

Design and Evaluation of Poly Herbal Mosquito Repellent Incense Sticks

The current study's goal was to create multi-herb mosquito-repellent incense sticks. Two formulations were made F1 and F2. The F1 formulation contains less quantity of poly herbal ingredients than the F2 formulation. The developed formulation's effectiveness as a mosquito repellent was assessed. The Incense mosquito sticks were prepared by hand rolling method in research laboratory. The herbal ingredients used are the Holy basil, Neem, Rosemary, Camphor, Cinnamon bark, lemongrass oil. The Acacia used as a binder to roll the sticks and lemon grass oil used as flavoring agent. The prepared formulation F1 and F2 was evaluated its efficacy. The F2 formulation containing increased quantity of poly herbs so it shows better repellent activity than F1 formulation. So the uses of natural herbs in the formulation of mosquito repellent sticks are free from carcinogenic chemicals, cheaper, and easy to formulate.

Formulation and Evaluation of Indomethacin Sustained Release Tablet by using Natural Polymers

Arthritis is most prevalent disorder and Indomethacin is choice of drug for arthritis. Oral route is most preferred route of drug administration and tablets are the more convenient dosage form. In present research natural polymers guar gum and xanthan gum were used to make sustained release tablet of Indomethacin. Indomethacin release from the tablets was studied in phosphate buffer pH 7.2. The drug release pattern of six different formulations in which xanthan gum and guar gum were used as a retarding material in different proportions was shown in table no. 6. The formulation F1 releases (75.20%) upto 12 hrs whereas the formulation F2 releases the drug (98.81%) in same time. The formulations F3, F4, F5 and F6 release 98.32%, 99.29%, 98.79% and 82.40% respectively. The dissolution pattern of both gums was similar i.e. with drug to polymer ratio (1:1). In present work F1 shows best sustained release upto 12 hrs in which Drug to Polymer ratio was 1:1:0 (Drug : Xanthan gum : Guar gum). In formulation F2 drug release was not sustained and complete release occurs in 12 hrs where the Drug to Polymer ratio was 1:1.67:0 (Drug: Xanthan gum : Guar gum). It was found that on the concentration of xanthan gum decreases the release rate of the drug also decreases. For sustain release there is not much role of Guar Gum reported. Indomethacin drug has been selected which has half-life 4.5 hrs. Hence the present work, an attempt has been made to provide sustained release drug delivery using polymers with Indomethacin as the model drug. Xanthan gum was best able to retard Indomethacin release mechanism even in the presence of a polymer. Polymer can be used to formulate successful sustained release Indomethacin matrix tablets that have desirable characteristics.

Formulation and Evaluation of Verapamil Hydrochloride Sustain Release Tablet

Verapamil hydrochloride, a calcium channel blocker, is used to treat hypertension, supraventricular arrhythmia and myocardial infarction Verapamil hydrochloride (VPH) sustain release tablets were developed for this study in order to maintain plasma concentrations, enhance bioavailability, and avoid repeated dosing. Materials and procedures Tablets containing a fixed amount of VPH were made using the direct compression method and various combinations and ratios of the polymers hydroxypropyl methyl cellulose (HPMC K15) and ethyl cellulose. The tablets were then tested for thickness, weight variation, hardness, uniformity of the drug content, drug release, and potential ingredient interactions. Regarding thickness, weight variation, hardness, and medication content, all tablets were satisfactory. It may be determined from formulations comprising HPMCK15 and ethyl cellulose that the two polymers functioned well together, controlling the first burst and improving the drug's release throughout the first three hours. A study of in vitro drug release followed the formulation of the 32 factorial planned batches. The formulations F5, F6, and F8 demonstrated better drug release up to 24hours and minimal burst release with more than 80% release in 24hours. They were composed of HPMC K15 60mg, 60 mg, and 75mg, respectively, and ethyl cellulose 30mg, 45mg, and 30mg, respectively. Thus, the F5 formulation with somewhat lower polymer content was chosen as the optimal formulation and they contained HPMC K15 60mg and ethyl cellulose 30mg.

Comparative Evaluation of Cefixime Microspheres Utilizing a Natural Polymer and a Synthetic Polymer

Background:: Microspheres are naturally biodegradable, free-flowing powders with a particle size of less than 200 micrometres that are comprised of proteins or synthetic polymers. Using microspheres is a reliable strategy to ensure that the drug is accurately delivered to the target area and that the right concentration is kept there without having any unfavourable side effects. Objective:: The objective of the present study was to create a sustained-release cefixime trihydrate microsphere delivery system employing natural and synthetic polymers as a carrier and increase therapeutic effectiveness. Methods:: Due to the simplicity of processing, the solvent injection method was used to create microspheres. Microspheres were created with this technology using the sustained-release polymer, sodium alginate, and active material (drug). The compatibility of components with the drug was evaluated using XRD and FT-IR. In an in-vitro release research, the dissolving medium was phosphate buffer at pH 6.8. For the kinetic analysis of the drug release mechanism, graphs for zero-order, first-order, Higuchi's, Korsmeyer-Peppas, and Hixson-Crowell models were also created. Results:: The best formulation was chosen from the batches, and in-vitro cefixime trihydrate release studies for various microspheres containing cefixime trihydrate in phosphate buffer (pH 7.4) for 8 hours were performed. The dissolution profiles of formulations F4 and F8 showed that the formulation, including xanthan gum, F8, released 55.01% more medication in 8 hours than the formulation using HPMC, F4. X-ray diffraction, swelling index of drug-laden microspheres, and Scanning Electron Microscopy were used to evaluate formulation F8. The graphs for zero-order, first-order, Higuchi's, Korsmeyer-Peppas, and Hixson- Crowell models were plotted, and the optimised batch was discovered to match Higuchi's drug release kinetics with an R2 value of 0.990. Conclusion:: Cefixime trihydrate microspheres can be utilized as a new drug delivery technology to minimize dose frequency and, as a result, to promote patient compliance.

Formulation and Evaluation of Pulsatile drug delivery of Albuterol sulphate and Theophylline drugs using modified Pulsincap technology

Albuterol Sulphate and Theophylline are used as anti-asthma agents. The objective of the present investigation was to design develop and evaluate a modified pulsincap drug delivery system of Albuterol Sulphate and Theophylline for the treatment of Asthma. The body of capsule was made insoluble with water by cross linking with formaldehyde. This modified capsule was completely filled with drug with polymer such as HPMCK15M, HPMC K 100M, PVPK-30 to expel the drug after predetermined lag time. A hydrogel plug was inserted in the body of a capsule to obtain desired drug release after a lag time for chronotherapy of asthma. Untreated capsule was attached to the treated body and was released. The drug was exerted to precompression parameter such as Angle of repose, Bulk density, Tapped density, Car’s index Hassner’s ratio. These capsules were subjected to further post formulation studies such as weight variation, drug content, invitro dissolution studies separately. The pre and post formulation parameters were found to be within permissible limit. The mere compatibility of drug, polymer and excipients were determined by Fourier transform infrared analysis (FTIR) and UV-Visible spectroscopy (UV). The results showed that drug was completely compatible with polymer and excipients. In vitro dissolution were carried out by using pH 6 .8 and pH 7.4 buffers. Based on the result obtained F5 Formulation shows good dissolution profile. Higuchi, Hixson crowell. Koresmeyer peppas to evaluate the kinetics and drug release. The drug release follows first order kinetics and the mechanism was found to be NON FICKS DIFFUSION.
 Keywords: Albuterol Sulphate, Theophylline, modified pulsincap drug delivery system, FTIR, Dissolution

Development and Characterization of Thermoreversible In-situ Gel Containing Curcumin Nanoparticles for Nasal Delivery: Design, Ex-Vivo Study

The present study aimed to develop and evaluate thermoreversible in situ nasal gel containing Curcumin nanoparticles. Curcumin nanoparticles were prepared by the ionic gelation method. These formulations were evaluated for entrapment efficiency, particle size, zeta potential, and In vitro drug release. Particle size and zeta potential of F1 formulation were found to be 299 nm, 0.1 mV, and entrapment efficiency was found to be87.2%. From the formulations, F1 shows maximum amount of drug released in a sustained manner for a prolonged period time. Hence F1formulation was selected as optimized and further used for thermoreversible in situ nasal gel by using 2×2 factorial designs. The thermoreversible nasal gel was evaluated for the gelation temperature (33˚C), viscosity (10550cps) and spreadability (17.29±0.3 gm*cm/sec). Further, the prepared gel (F1G3) was evaluated for ex vivo permeation study for12 hours and it shows maximum amount of drug release in a controlled manner. The % CDR of thermoreversible in situ gel formulation F1G3 was found to be 86.27% which follow the Higuchi model. The 'n' value of the formulation was found to be more than 0.5. This indicate that the release approximates the non-fickain diffusion mechanism.
 Keywords: Curcumin nanoparticles, Carbopol 934, PF127, Thermoreversible in situ gel, Cold method, Nasal delivery.

Formulation and development of topical iron oxide nanoemulgel using Punica granatum extract and in vitro evaluation of anti-inflammatory potential in rheumatoid arthritis

Abstract Rheumatoid Arthritis (RA) is regarded as a long-term autoimmune disorder, which triggers inflammation in joints and distresses the organs in the body. Nanotechnology, a science-based technique is a tremendously growing-field with numerous applications where nanoparticles are used to resolve the complications in the habitual life. While comparing to other metals, iron oxide nanoparticles (Fe2O3 NPs) were synthesized at minimal cost using eco-friendly natured materials and also has ability to deliver drug at a specific targeted site. In the present study, a topical nanoemulgel was formulated using Fe2O3 NPs synthesized from Punica granatum. The results of Fe2O3 NPs characterization divulged that the particles were sphere-shaped in nature and the bioactive compounds from the extract acted as capping and reducing agents. Nanoemulgels (F1 to F5) were formulated using different concentrations of Fe2O3 NPs and characterized. Among the formulations F4 nanoemulgel revealed good stability with a zeta potential charge of −53 mV and a polydispersity index of 0.24. L929 cells were treated with F4 formulation and this confirmed that the formulation was non-toxic to normal cells. F4 treated MG63 cell lines revealed the anti-inflammatory property (IC50 = 90.77 μg/ml) of the nanoemulgel. Thus, nanoemulgel containing Fe2O3 NPs synthesized from P. granatum can be an effective, eco-friendly and cost-effective approach to treat RA.

Fabrication and Characterization of Fast Dissolving Herbal Buccal Film Containing Mimosa Pudica Leaf Extract

Buccal films has distinct advantages over conventional dosage forms. Drugs can be delivered through buccal route, by avoiding first pass metabolism to produce local and systemic action. Rapid absorption of the drug is achieved because buccal mucosa is highly permeable with rich blood supply. Only few dosage forms are designed to deliver the drug through this route. Leaf extract of Mimosa pudica has number of pharmacological actions. Buccal film of leaf extract of mimosa pudica is prepared by solvent casting method and evaluated for its characteristics. Six formulations were prepared, out of which F3 formulation was found promising formulation, releases 96.13% of drug in 10 minutes.