Formation Rate Research Articles

The effects of stellar and AGN feedback on the cosmic star formation history in the simba simulations

ABSTRACT Using several variants of the cosmological simba simulations, we investigate the impact of different feedback prescriptions on the cosmic star formation history. Adopting a global-to-local approach, we link signatures seen in global observables, such as the star formation rate density (SFRD) and the galaxy stellar mass function (GSMF), to feedback effects in individual galaxies. We find a consistent picture: stellar feedback mainly suppresses star formation below halo masses of $M_{\rm H} = 10^{12} \rm \, {\rm M}_{\odot }$ and before $z = 2$, whereas AGN feedback quenches the more massive systems after $z = 2$. Among simba’s AGN feedback modes, AGN jets are the dominant quenching mechanism and set the shape of the SFRD and the GSMF at late times. AGN-powered winds only suppress the star formation rate in intermediate-mass galaxies ($M_{\rm \star } = 10^{9.5 - 10} \rm \, {\rm M}_{\odot }$), without affecting the overall stellar mass-assembly significantly. At late times, the AGN X-ray feedback mode mainly quenches residual star formation in massive galaxies. Our analysis reveals that this mode is also necessary to produce the first fully quenched galaxies before $z=2$, where the jets alone are inefficient. These initially highly star-forming galaxies contain relatively large black holes, likely strengthening the X-ray-powered heating and ejection of gas from the dense, central region of galaxies. Such extra heating source quenches the local star formation and produces a more variable accretion rate. More generally, this effect also causes the break down of correlations between the specific star formation rate, the accretion rate and the black hole mass.

Leaf-like MSX/TiN heterojunction photocathodes mimicking plant cell – An effective strategy to enhance photoelectrocatalytic carbon dioxide reduction and systematic mechanism investigation

Semiconductors, such as metal oxides and metal sulfides (MSX), are widely investigated as effectively catalytic materials to convert carbon dioxide (CO2) and water into chemicals under simulated solar light. These valuable investigations might address both the energy crisis and climate change in our modern society. Herein, a novel strategy to construct leaf-like heterojunctions of VS-ZnIn2S4/TiN-x is reported. The new semiconductor heterojunctions were then applied to photoelectrocatalytic CO2 reduction, achieving excellent performance (formate formation rate of 1173.2 μM h−1 cm−2) attributed to the plant cell-like morphology and enhanced electron mobility from the heterojunction interfaces to the active sites on the surface. Our findings suggest that titanium nitride (TiN) with good conductivity can improve the photoelectrocatalytic ability of MSX through heterojunction construction. The photocathode VS-ZnIn2S4/TiN-3 exhibits 81.0 % selectivity toward C2 products by optimizing the material structure and reaction conditions. According to the systematic investigation of operando Fourier transform infrared (FTIR) spectra, common intermediates such as *COO−, *COOH, *CO, *CHO, *COCHO, and *COCH3 reported in the literature were carefully verified. Among these, the carbene specie serve as the key intermediate responsible for generating other intermediates and resulting in all products.

Quantitative analysis of the micromechanical behavior and work hardening in Fe-0.1C–10Mn steel via in-situ high-energy X-ray diffraction

In the current work, the micromechanical behavior and work hardening behavior of Fe-0.1C–10Mn (in wt.%) steel deformed at 100, 63, 25 and −50 °C were investigated via in-situ high-energy X-ray diffraction (HE-XRD) technique. As the deformation temperature decreased, the yield strength (YS) and ultimate tensile strength (UTS) increased, while the total elongation (TE) reached a maximum value at 25 °C. The transformation kinetics of retained austenite (RA) was fitted by the Olson and Cohen (OC) model. The phase stress and flow stress contributed by the constituent phases were obtained based on the lattice strain and the volume fraction of the corresponding phase. The work hardening rate was decomposed into four contributors related to the TRIP effect and load partitioning, ie., the austenite phase stress, load partitioning between austenite and martensite, martensitic formation rate and load partitioning between ferrite and austenite. The influence of each contributor on the work hardening behavior was quantitatively evaluated and stacked, the stacked results agreed reasonably well with the experimental work hardening rate obtained from the true stress-strain curve. Finally, the volume fraction of austenite to martensite transformation promoted by the Lüders band (LB) and the stacking fault energy (SFE) of RA were found to be highly temperature-dependent. A linear relationship was revealed between the volume fraction of austenite to martensite transformation during the LB propagation and the SFE of RA. These findings offer insights into the TRIP effect and the LB propagation in medium-Mn steels.

Towards a universal analytical model for Population III star formation: interplay between feedback and fragmentation

ABSTRACT JWST has brought us new insights into Cosmic Dawn with tentative detection of the unique signatures of metal-free Population III (Pop III) stars, such as strong He II emission, extremely blue ultraviolet spectrum, and enhanced nitrogen abundance. Self-consistent theoretical predictions of the formation rates, sites, and masses of Pop III stars are crucial for interpreting the observations, but are challenging due to complex physical processes operating over the large range of length-scales involved. One solution is to combine analytical models for the small-scale star formation process with cosmological simulations that capture the large-scale physics such as structure formation, radiation backgrounds, and baryon-dark matter streaming motion that regulate the conditions of Pop III star formation. We build an analytical model to predict the final masses of Pop III stars/clusters from the properties of star-forming clouds, based on the key results of small-scale star formation simulations and stellar evolution models. Our model for the first time considers the interplay between feedback and fragmentation and covers different modes of Pop III star formation ranging from ordinary small ($\sim\!{10{-}2000}\ \rm M_\odot$) clusters in molecular-cooling clouds to massive ($\gtrsim\!{10^{4}}\ \rm M_\odot$) clusters containing supermassive ($\sim\!{10^{4}{-}3}\times 10^{5}\ \rm M_\odot$) stars under violent collapse of atomic-cooling clouds with large gas accretion rates of $\gtrsim\!{0.1}\ \rm M_\odot \ yr^{-1}$. As an example, the model is applied to the Pop III star-forming clouds in the progenitors of typical haloes hosting high-z luminous quasars ($M_{\rm h}\sim 10^{12}\ \rm M_\odot$ at $z\sim 6$), which shows that formation of Pop III massive clusters is common ($\sim\!{20{-}70}{{\ \rm per\ cent}}$) in such biased ($\sim\!{4}\sigma$) regions, and the resulting heavy black hole seeds from supermassive stars can account for a significant fraction of observed luminous ($\gtrsim\!{10^{46}}\ \rm erg\ s^{-1}$) quasars at $z\sim 6$.

WP5.6 - Beyond the Left Side: Emergency Colonic Stenting for Acute Malignant Large Bowel Obstruction

Abstract Introduction Acute malignant large bowel obstruction (LBO) is traditionally treated by surgical diversion or resection in patients who are fit enough. European guidelines suggest colonic stenting as a bridge to surgery in curative disease. The aim was to examine the safety and efficacy of a change in practice from emergency laparotomy towards colonic stenting for acute malignant LBO. Methods Patients with LBO from 01/01/2019 to 31/12/2022 were included and data was obtained from the NELA database and electronic patient records. Results 42 patients underwent emergency laparotomy (EL) for LBO. 33% (14/42) patients had right-sided tumours (n=14) and/or perforated disease (n=6) where stenting was contraindicated. 28/42 patients (22% of total) went straight to EL for clinical/ organisation factors. 73 patients were stented; 44% (32/73) were at/ proximal to splenic flexure. Perforation rate was 10% (7/73) with 4/7 requiring EL. Failure rate was 14% (10/73) with 6/10 patients requiring EL. 41% (30/73) were stented with curative intent; 70% (21/30) then underwent elective resection. The rate of stoma formation and minimally invasive surgery in patients stented with curative intent was 40% (12/30) and 60% (18/30) respectively (including 9 EL following stent complications). Compared with 88% (37/42) and 19% (8/42) in patients undergoing primary EL. Conclusion Almost 2/3 of patients presenting with acute malignant LBO were suitable for stenting. Stenting reduced the need for a stoma and enabled elective minimally invasive surgery with low complication rates. This rate of colonic stenting was achievable without a 24/7 colonic stenting rota or sub-specialty cover.

Rejuvenation of aged oocyte through exposure to young follicular microenvironment.

Reproductive aging is a major cause of fertility decline, attributed to decreased oocyte quantity and developmental potential. A possible cause is aging of the surrounding follicular somatic cells that support oocyte growth and development by providing nutrients and regulatory factors. Here, by creating chimeric follicles, whereby an oocyte from one follicle was transplanted into and cultured within another follicle whose native oocyte was removed, we show that young oocytes cultured in aged follicles exhibited impeded meiotic maturation and developmental potential, whereas aged oocytes cultured within young follicles were significantly improved in rates of maturation, blastocyst formation and live birth after in vitro fertilization and embryo implantation. This rejuvenation of aged oocytes was associated with enhanced interaction with somatic cells, transcriptomic and metabolomic remodeling, improved mitochondrial function and higher fidelity of meiotic chromosome segregation. These findings provide the basis for a future follicular somatic cell-based therapy to treat female infertility.

The effect of high-normal preoperative international normalized ratios on postoperative outcomes and complications following posterior cervical spine surgery

IntroductionCurrent guidelines recommend that the International Normalized Ratio (INR) be less than 1.5 prior to spine intervention. Recent studies have shown that an INR > 1.25 is associated worse outcomes following anterior cervical surgery. We sought to determine the risk of complications associated with an INR > 1.25 following elective posterior cervical surgery.MethodsThe American College of Surgeons National Surgical Quality Improvement Program database was queried. Patients undergoing elective posterior cervical surgery from 2012 to 2016 with an INR level within 24 h of surgery were included. Primary outcomes were hematoma requiring surgery, 30-day mortality, and transfusions within 72-hours. There were 815 patients in the INR ≤ 1 cohort (Cohort A), 410 patients in the 1 < INR ≤ 1.25 cohort (Cohort B), and 33 patients in the 1.25 < INR ≤ 1.5 cohort (Cohort C).ResultsCohort C had a higher rate of transfusion (4% Cohort A; 6% Cohort B; 12% Cohort C; p = 0.028) and the rate of mortality within 30 days postoperatively trended toward significance (0.4% Cohort A; 0.5% Cohort B; 3% Cohort C; p = 0.094). There was no significant difference in the rate of postoperative hematoma formation requiring surgery (0.2% Cohort A; 0% Cohort B; 0% Cohort C; p = 0.58). On multivariate analysis, increasing INR was not associated with an increased risk of developing a major complication.ConclusionAn INR > 1.25 but ≤ 1.5 may be safe for posterior cervical surgery. An INR > 1.25 but ≤ 1.5 was associated with a significantly higher rate of transfusions. However, increasing INR was not significantly associated with increased risk of any of the major complications.

FP3.15 - Are non-colorectal surgeons more likely to make a stoma in an emergency right hemicolectomy for cancer?

Abstract Introduction Right sided colonic cancers can often cause bowel obstruction and present as an emergency. The decision to perform an anastomois following a right hemicolectomy resection is frequently multifactorial. There is controversy as to whether colorectal cancers should be operated on by non-colorectal surgeons in the emergency setting. This study explores whether there are differences in stoma formation rates between colorectal and non-colorectal surgeons in the context of emergency right hemicolectomy operations performed for malignancy. Methods A retrospective study was conducted at a 3 site Trust in South East England between 2017 and 2022. Theatre and Infoflex Cancer records were used to obtain patients who had undergone an open right hemicolectomy for malignancy and their operating surgeon. Results There were 281 patients included in the study, in a 152:129 female:male ratio. The mean age was 68 (34-86 IQR). 13% (37/281) of operations were conducted by Upper Gastro-intestinal Surgeons. 54% (20/37) of these patients had stoma formation as part of their operation. 47% (115/244) of operations undertaken by colorectal surgeons also had stoma formation. There was no significant difference between the stoma formation between colorectal and non-colorectal surgeons (p=0.108) Conclusion The study shows that although the decision to form a stoma following a right hemicolectomy multifactorial and is dependent on a wealth of patient and operative factors, there is no statistical difference between colorectal and non-colorectal surgeons in the emergency setting.

A targeted search for strongly lensed supernovae with the Las Cumbres Observatory

Abstract Gravitationally lensed supernovae (glSNe) are of interest for time delay cosmology and SN physics. However, glSNe detections are rare, owing to the intrinsic rarity of SN explosions, the necessity of alignment with a foreground lens, and the relatively short window of detectability. We present the Las Cumbres Observatory Lensed Supernova Search, LCOLSS, a targeted survey designed for detecting glSNe in known strong lensing systems. Using cadenced r′-band imaging, LCOLSS targeted 114 galaxy-galaxy lensing systems with high expected SN rates, based on estimated star formation rates. No plausible glSN was detected by LCOLSS during our two year observing program. We carry out an analysis here to measure a detection efficiency for these observations. We then perform Monte Carlo simulations using the predicted supernova rates to determine the expected number of glSN detections. The results of the simulation suggest an expected number of detections and $68\%$ Poisson confidence intervals, NSN = 0.20, [0, 2.1], NIa = 0.08, [0, 2.0], NCC = 0.12, [0, 2.0], for all SNe, Type Ia SNe, and core-collapse (CC) SNe respectively. These results are broadly consistent with the absence of a detection in our survey. Analysis of the survey strategy can provide insights for future efforts to develop targeted glSN discovery programs, especially considering the large anticipated yields of upcoming surveys.

Atmospheric Bases-Enhanced Iodic Acid Nucleation: Altitude-Dependent Characteristics and Molecular Mechanisms.

Iodic acid (IA), the key driver of marine aerosols, is widely detected within the gas and particle phases in the marine boundary layer (MBL) and even the free troposphere (FT). Although atmospheric bases like dimethylamine (DMA) and ammonia (NH3) can enhance IA particles formation, their different efficiencies and spatial distributions make the dominant base-stabilization mechanisms of forming IA particles unclear. Herein, we investigated the IA-DMA-NH3 nucleation system through quantum chemical calculations at the DLPNO-CCSD(T)/aug-cc-pVTZ(-PP)//ωB97X-D/6-311++G(3df,3pd) + aug-cc-pVTZ-PP level of theory and cluster dynamics simulations. We provide molecular-level evidence that DMA and NH3 can jointly stabilize the IA clusters. The formation rates of IA clusters initially decline before rising from the MBL to the FT, owing to variations in mechanism. In the MBL, IA-DMA nucleation predominates, while the contribution of IA-DMA-NH3 synergistic nucleation cannot be overlooked in polar and NH3-polluted regions. In the lower FT, IA-DMA-NH3 nucleation prevails, whereas in the upper FT, IA-NH3 nucleation dominates. The efficiency of IA-DMA-NH3 nucleation is comparable to that of IA-iodous acid nucleation in the MBL and sulfuric acid-NH3 nucleation in the FT. Hence, the IA-DMA-NH3 mechanism holds promise for revealing the missing sources of tropospheric IA particles.

Supervised star, galaxy, and QSO classification with sharpened dimensionality reduction

We explored the use of broadband colors to classify stars, galaxies, and quasi-stellar objects (QSOs). Specifically, we applied sharpened dimensionality reduction (SDR)-aided classification to this problem, with the aim of enhancing cluster separation in the projections of high-dimensional data clusters to allow for better classification performance and more informative projections. The main objective of this work was to apply SDR to large sets of broadband colors derived from the CPz catalog to obtain projections with clusters of star, galaxy, and QSO data that exhibit a high degree of separation. The SDR method achieves this by combining density-based clustering with conventional dimensionality-reduction techniques. To make SDR scalable and have the ability to project samples using the earlier-computed projection, we used a deep neural network trained to reproduce the SDR projections. Subsequently classification was done by applying a $k$-nearest neighbors ($k$-NN) classifier to the sharpened projections. Based on a qualitative and quantitative analysis of the embeddings produced by SDR, we find that SDR consistently produces accurate projections with a high degree of cluster separation. A number of projection performance metrics are used to evaluate this separation, including the trustworthiness, continuity, Shepard goodness, and distribution consistency metrics. Using the $k$-NN classifier and consolidating the results of various data sets, we obtain precisions of 99.7&lt;!PCT!&gt;, 98.9&lt;!PCT!&gt;, and 98.5&lt;!PCT!&gt; for classifying stars, galaxies, and QSOs, respectively. Furthermore, we achieve completenesses of 97.8&lt;!PCT!&gt;, 99.3&lt;!PCT!&gt;, and 86.8&lt;!PCT!&gt;, respectively. In addition to classification, we explore the structure of the embeddings produced by SDR by cross-matching with data from Gaia DR3, Galaxy Zoo 1, and a catalog of specific star formation rates, stellar masses, and dust luminosities. We discover that the embeddings reveal astrophysical information, which allows one to understand the structure of the high-dimensional broadband color data in greater detail. We find that SDR-aided star, galaxy, and QSO classification performs comparably to another unsupervised learning method using hierarchical density-based spatial clustering of applications with noise (HDBSCAN) but offers advantages in terms of scalability and interpretability. Furthermore, it outperforms traditional color selection methods in terms of QSO classification performance. Overall, we demonstrate the potential of SDR-aided classification to provide an accurate and physically insightful classification of astronomical objects based on their broadband colors.

The effect of mass and morphology on the mass assembly of galaxies

The pace at which galaxies grew into their current stellar masses and how this growth is regulated is still not fully understood, nor is the role that morphology plays in this process. We applied full spectral fitting techniques with pyPipe3D to the MaNGA sample to obtain its star formation and stellar mass histories and used these to investigate the mass assembly of galaxies by measuring how their specific star formation correlates to their stellar mass at different look-back times. We find that the correlation between these two parameters was shallower in the past. Galaxies used to have similar mass doubling times and the current negative correlation between the specific star formation and M$_ is primarily due to more massive galaxies 'dropping' off the main sequence earlier than less massive ones. Additionally, selecting the galaxies into bins based on their present-day morphology shows a segregation in specific star formation rate (sSFR) that is maintained even at high look-back times, showing that the factors that determine which morphology a galaxy ends up in are in place at very early times. Similarly, selecting them based on their current star formation status shows that, on average, currently retired galaxies used to have slightly a higher sSFR before the drop-off, whereas galaxies that have continued to form stars until today had a lower sSFR initially. We compare our results to a set of cosmic surveys, finding partial agreement in our results with several of them, though with significant offsets in redshift. Finally, we discuss how our results fit with certain theoretical models on galaxy evolution as well as cosmological simulations.

The effect of fluence rate and wavelength on the formation of protoporphyrin IX photoproducts.

Photodynamic diagnosis and therapy (PDD and PDT) are emerging techniques for diagnosing and treating tumors and malignant diseases. Photoproducts of protoporphyrin IX (PpIX) used in PDD and PDT may be used in the diagnosis and treatment, making a detailed analysis of the photoproduct formation under various treatment and diagnosis conditions important.Spectroscopic and mass spectrometric analysis of photoproduct formation from PpIX dissolved in dimethyl sulfoxide were performed under commonly used irradiation conditions for PDD and PDT, i.e., wavelengths of 405 and 635nm and fluence rates of 10 and 100 mW/cm2. Irradiation resulted in the formation of hydroxyaldehyde photoproduct (photoprotoporphyrin; Ppp) and formyl photoproduct (product II; Pp II) existing in different quantities with the irradiation wavelength and fluence rate. Ppp was dominant under 635nm irradiation of PpIX, with a fluorescence peak at 673nm and a protonated monoisotopic peak at m/z 595.3. PpIX irradiation with 405nm yielded more Pp II, with a fluorescence peak at 654nm. A higher photoproduct formation was observed at a low fluence rate for irradiation with 635nm, while irradiation with 405nm indicated a higher photoproduct formation at a higher fluence rate.The photoproduct formation with the irradiation conditions can be exploited for dosimetry estimation and may be used as an additional photosensitizer to improve the diagnostics and treatment efficacies of PDD and PDT. Differences in environmental conditions of the present study from that of a biological environment may result in a variation in the photoproduct formation rate and may limit their clinical utilization in PDD and PDT. Thus, further investigation of photoproduct formation rates in more complex biological environments, including in vivo, is necessary. However, the results obtained in this study will serve as a basis for understanding reaction processes in such biological environments.

Temperature-dependent akinete formation strategies of the harmful cyanobacterium Dolichospermum circinale

Cyanobacteria from the orders Nostocales and certain Stigonematales form akinetes, spore-like dormant cells that allow them to survive adverse environmental conditions. Temperature is known to be one of the key factors affecting akinete formation, but there is currently little known about akinete formation during cell growth over a wide range of temperature conditions and its relation to the overall survival strategy of cyanobacteria. Therefore, in the current study, we conducted a temperature-controlled experiment to analyze the akinete formation of a harmful cyanobacterium Dolichospurmum circinale using a growth chamber. We measured the concentration and size of both vegetative cells and different types of akinetes (free, attached, and empty type) under varying temperatures (5–25 °C). We also analyzed the buoyant ability and vertical migration velocity of trichomes along with changes in the volume of vegetative cells and akinetes. The total akinete concentration and ratio (number of akinetes to total number of cells) were both found to be higher at high temperatures (20–25°C) than they were at low temperatures (5–10°C) (p<0.05). Meanwhile, the rate of formation of akinetes (both free and attached akinetes) was highest at low temperature (10 °C) and decreased with increasing temperature. The rate of empty akinete formation increased with increasing temperature and was highest at 25°C, indicating that most of the akinetes produced under high temperature conditions germinated. The change in vegetative cell size was proportional to the increase in the growth rate in response to increasing temperature (p<0.05). At high temperature, vegetative cells exhibited positive buoyancy and higher vertical migration velocity, while at low temperature, they exhibited negative buoyancy and relatively low migration velocity. Akinete size was larger at low temperature than it was at high temperature. These findings suggest that akinetes play an important role in maintaining populations in the water column, with a link between akinete formation and germination during summer cyanobacteria blooms. This information is expected to contribute to a deeper understanding of the D. circinale life cycle.

Pig-derived ECM-SIS provides a novel matrix gel for tumor modeling

The absence of effective extracellular matrix to mimic the natural tumor microenvironment remains a significant obstacle in cancer research. Matrigel, abundant in various biological matrix components, is limited in its application due to its high cost. This has prompted researchers to explore alternative matrix substitutes. Here, we have investigated the effects of the extracellular matrix derived from pig small intestinal submucosa (ECM-SIS) in xenograft tumor modeling. Our results showed that the pig-derived ECM-SIS effectively promotes the establishment of xenograft tumor models, with a tumor formation rate comparable to that of Matrigel. Furthermore, we showed that the pig-derived ECM-SIS exhibited lower immune rejection and fewer infiltrating macrophages than Matrigel. Gene sequencing analysis demonstrated only a 0.5% difference in genes between pig-derived ECM-SIS and Matrigel during the process of tumor tissue formation. These differentially expressed genes primarily participate in cellular processes, biological regulation, and metabolic processes. These findings emphasize the potential of pig-derived ECM-SIS as a cost-effective option for tumor modeling in cancer research.

SDSSD peptide modified polyvinylamine − A novel bone-targeting RNA delivery system

Bone-related disorders have become a prevailing concern in older men and postmenopausal women. Traditional methods of treatment, though have benefits, their inability to target specific cells or tissues increases their ineffectiveness and side effects. RNA therapy has emerged as an alternative to conventional therapy owing to its specific targeting ability. However, the lack of appropriate RNA vectors remains a major problem for successful RNA therapy. Here, we designed a delivery system, (SDSSD-PVAm (PS)) based on a cationic polymer, polyvinylamine (PVAm), and a bone-targeting peptide, SDSSD (Serine-Aspartate-Serine-Serine-Aspartate), to deliver a therapeutic RNA, anti-miR-138-5p, to the bone tissue. The results showed that the SDSSD modification not only had no negative effect on the RNA-carrying or protection abilities of PVAm but also improved targeting and RNA delivery to osteoblasts and improve osteogenic potential of the cells by improving the expression of osteogenic genes both at RNA and protein level. Recombinant anti-miR-138-5p delivered through PS was highly enriched in the bone tissue, and the resultant nanoparticles could circulate for a longer time in the blood with negligible toxicity. The results showed that improved RNA delivery through PS significantly increased the rate of bone formation and improved trabecular and cortical bone microarchitecture, resulting in enhanced bone mass in ovariectomy (OVX) and hind limb unloading (HLU) model mice. These results demonstrate that SDSSD-PVAm is a novel RNA delivery system for bone-related disorders, such as osteoporosis.

Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu.

Macromolecular crowding bridges in vivo and in vitro studies by simulating cellular complexities such as high viscosity and limited space while maintaining the experimental feasibility. Over the last two decades, the impact of macromolecular crowding on protein stability and activity has been a significant topic of study and discussion, though still lacking a thorough mechanistic understanding. This article investigates the role of associated water dynamics on protein stability and activity within crowded environments, using bromelain and Ficoll-70 as the model systems. Traditional crowding theory primarily attributes protein stability to entropic effects (excluded volume) and enthalpic interactions. However, our recent findings suggest that water structure modulation plays a crucial role in a crowded environment. In this report, we strengthen the conclusion of our previous study, i.e., rigid-associated water stabilizes proteins via entropy and destabilizes them via enthalpy, while flexible water has the opposite effect. In the process, we addressed previous shortcomings with a systematic concentration-dependent study using a single-domain protein and component analysis of solvation dynamics. More importantly, we analyze bromelain's hydrolytic activity using the Michaelis-Menten model to understand kinetic parameters like maximum velocity (Vmax) achieved by the system and the Michaelis-Menten coefficient (KM). Results indicate that microviscosity (not the bulk viscosity) controls the enzyme-substrate (ES) complex formation, where an increase in the microviscosity makes the ES complex formation less favorable. On the other hand, flexible associated water dynamics were found to favor the rate of product formation significantly from the ES complex, while rigid associated water hinders it. This study improves our understanding of protein stability and activity in crowded environments, highlighting the critical role of associated water dynamics.

Tuning the structural and optical parameters of Li1.2Ni0.4Fe2O4 for efficient degradation of dye and pharmaceutical compounds

Sol-gel auto-combustion route was used for the fabrication of lithium and dysprosium co-doped nickel ferrite (LNDFO). The composite of LNDFO with gCN (LNDFO@gCN) was prepared via ultra-sonication method. The synthesized samples were tested by photodegrading organic pollutants. Different characterization techniques were employed to examine the structure, morphology, elemental composition, and bandgap of prepared photocatalysts. The optical bandgap energy of LNFO, LNDFO, and LNDFO@gCN was 2.92 eV, 2.73 eV, and 2.64 eV, respectively. LNDFO@gCN had the lowest recombination rate of charged species and greater surface area, which makes it an efficient photocatalyst. The degradation shown by LNDFO@gCN for panadol, MB, and phenol was 90.04 %, 93 %, and 89.5 % respectively. EIS and Mott-Schottky analysis revealed that the charge transfer resistance of fabricated composite was less than bare and doped samples, and they were n-type semiconductor materials. As compared to all fabricated photocatalysts, LNDFO@gCN was most efficient photocatalyst due to its high degradation ability, easy formation, and higher charge separation rate.

The effect of resveratrol on the developmental competence of feline oocytes vitrified at the metaphase II stage

The objective of this research was to assess the viability and developmental potential of feline oocytes following in vitro maturation (IVM), vitrification, and post-warming incubation with resveratrol. In the first experiment, warmed oocytes were incubated with 0.2 μM, 2 μM, or 20 μM resveratrol for 2 h. Oocytes treated with 0.2 μM resveratrol had the highest viability (68.89 %), as assessed by fluorescein diacetate and ethidium bromide staining, while higher concentrations were associated with diminished oocyte viability. In the second experiment, the warmed oocytes were inseminated following the 2-h incubation with the three concentrations of resveratrol. The presumptive zygotes were then maintained in culture and their development evaluated. The highest cleavage rate was observed when the oocytes had been incubated with 0.2 μM resveratrol (88.34 %), which was higher than for the control group (without resveratrol (75 %)). Moreover, this concentration of resveratrol also augmented the blastocyst formation rate. While the vitrification of oocytes often results in diminished developmental potential in the ensuing embryos, attributed to cryopreservation-induced injury, the utilization of low concentrations of resveratrol enhances the procedure's efficacy.

Ceria-boosted Ni/Al2O3 catalysts for enhanced H2 production via acetic acid dry reforming

Acetic acid dry reforming (ADR) is a promising route for sustainable H2 generation. However, coke inhibition during ADR is the main challenge and not resolved by using suitable promoted catalysts. In this work, Ce promotion on 10%Ni/Al2O3 catalysts with 1-5 wt%Ce was evaluated for ADR at varied temperatures of 923–998 K and stoichiometric feed in a fixed-bed rig. CeO2 addition of 1–3% enhanced metal dispersion, and surface area whilst basic CeO2 character significantly boosted the concentration and density of basic sites on catalysts. Particularly, the CO2 uptake of promoted catalysts was about 2.49–3.73 times greater than that of counterpart sample. CH3COOH and CO2 conversions were enhanced with rising Ce loading and the highest reactant conversions were observed at 3 wt%Ce. The improved adsorption of acidic CH3COOH and CO2 molecules due to increasing amount of basic sites as well as redox attributes of CeO2 promoter could be responsible for the enhancement in ADR activity and yield of H2 and CO. The mechanistic two-step pathway for coke suppression induced by CeO2 promotion was elaborated in this work. Generally, carbonaceous species formation on 3%Ce–10%Ni/Al2O3 was considerably reduced about 1.6–2.0 times. H2/CO ratio varied from 0.59 to 0.65 relying on ADR temperature over 3%Ce–10%Ni/Al2O3. These H2/CO values, two times higher than theoretical H2/CO ratio in ADR, are compatible for downstream gas-to-liquid processes to selectively yield high molecular weight olefins. Water formation rate increased from 8.67 × 10−6 to 4.71 × 10−5molH2O gcat−1 s−1 with rising temperature within 923–998 K on 3%Ce–10%Ni/Al2O3.