Hepatoma Formation Research Articles

Nucleodepolymerase activity in regenerating rat liver.

The desoxyribonucleodepolymerase and ribonucleodepolymerase activity has been estimated in rat liver at various time intervals following partial hepatectomy. When expressed per a constant amount of nitrogen of the tissue, as routinely done, the activity of these enzymes shows significant variations in regenerating rat liver. However, these variations are no longer evident when the enzymatic activity is recalculated “per cell” and it is concluded that they do not reflect actual variations of enzymatic activity in the average cell of the regenerating rat liver. Presumably, the increase in activity of the two nucleode-polymerases, previously observed in liver of rats fed p-dimethylaminoazobenzene, should be attributed to the liver injury by the azo dye rather than to the ensuing regeneration with ultimate hepatoma formation.

LIVER TUMOURS PRODUCED BY SIX ISOMERIC AMINOAZOTOLUENES

SYNOPSIS.Six isomeric amino‐azotoluenes have been fed to different groups of rats and mice, to test the influence of slight changes of chemical structure on carcinogenic activity.Liver damage was produced earlier in mice than rats, but the relative potency of the six isomers was similar for both species. Only two isomers–o: o and o: p‐induced liver tumours in rats and mice, but p: m and m: m were also carcinogenic, in lesser degree, in mice alone.The sequence of histological changes seen in the liver is described and pictured. In rat livers perilobular necrosis was succeeded by regeneration in the necrotic areas with microscopic hepatoma formation at some of these sites, culminating in malignant hepatoma or, occasionally, cholangioma. In mouse livers no preliminary necrosis occurred, but irregularities in cellular pattern and size of nuclei were followed by microscopic hepatoma or pronounced proliferation of bile ducts, with the final emergence of malignant hepatoma or cholangioma, or both.

Alterations in nucleic acids during hepatoma formation in rats fed p-dimethylaminoazobenzene

Alterations in nucleic acids during hepatoma formation in rats fed p-dimethylaminoazobenzene