Pellet Form Research Articles

Antimicrobial Solid Starch-Iodine Complex via Reactive Extrusion and Its Application in PLA-PBAT Blown Films.

In this study, a solid masterbatch of starch-iodine complex with 6.7 wt.% iodine was prepared in pellet form using a ZSK-30 twin-screw extruder. Thermogravimetric (TGA) and isothermal TGA analysis of the pellets revealed that there was no significant loss of iodine due to sublimation during reactive extrusion. These solid pellets demonstrated antifungal properties when applied to strawberries via dip coating in an aqueous solution, extending their shelf life from two days to eight days, thereby reducing fungal growth and visual decay. Furthermore, the solid pellets displayed antibacterial activity against E. coli, as evidenced by the clear zone of inhibition observed in the Kirby-Bauer test. To enhance practical application, these pellets were further blended with PLA-PBAT film formulations at 10 and 18% by wt. to make blown films with effective iodine loadings of 0.7 and 1.3% by wt. These films showed superior antibacterial activity against E. coli compared with PLA control films and the commercial silver antimicrobial-containing films during direct inoculation tests as per ISO 22196. Tensile strength and elongation at break in machine direction (MD) for the starch-iodine-containing blown films were comparable to the control films in MD, but tensile strength was reduced to 37-40% in the transverse direction (TD). This was due to a non-uniform dispersion of the starch-iodine complex in the films, as confirmed by the visual and SEM analyses. Thus, this study illustrates the practical utility of the solid starch-iodine complex as a safe and efficient means of introducing iodine into an environment, mitigating the typical hazards associated with handling solid iodine.

Transmittance estimation of translucent polymeric pellets

AbstractPlastics are commonly produced and sold in pellet form due to their superior handling characteristics. However, due to their small size, it is often impractical, if not unfeasible, to determine the transmittance of a single pellet instrumentally. Moreover, such measurements may be highly variable. Therefore, translucent films of certain thickness, known as plaques, are commonly molded to enable instrumental determination of their transmittance. These plaques, however, are not needed beyond the quality control process while they add a costly step to the production process. In this study, we test a method, based on the layer theory, that enables the estimation of the transmittance spectra of nearly transparent plastic plaques from the reflectance measurements of their pellet counterparts. The comparison of the estimated transmittance spectra of pellets versus measured transmittance of plaques shows the RMSE ranging from 0.37%–1.80%, with a color difference, CIEDE2000(1:1:1), of 0.07–0.48, thus validating the applicability of the method.

Characterization and removal of microplastics in the Guheshwori Wastewater Treatment Plant, Nepal

Contamination of river water systems by microplastic particles (MPPs) is one of the emerging global environmental concerns with potentially widespread ecological, socioeconomic, and health implications. A wastewater treatment plant (WWTP) processes and treats wastewater to remove pollutants and release safe water into the environment. There has been limited research on the characterization of microplastics and their removal in WWTP in South Asia. In this work, we report on the characterization of microplastics in wastewater and sludge samples (n = 300) from Guheshwori WWTP located on the bank of the Bagmati River in Kathmandu city, Nepal representing inlet, secondary aeration tank (SAT), outlet, and sludge from November 2021 to November 2022. On average, we detected 31.2 ± 17.3 MPPs/L, 11.2 ± 9.4 MPPs/L, 8.5 ± 5.6 MPPs/L, and 6.6 ± 4.8 MPPs/g in the samples collected from inlet, SAT, outlet, and sludge, respectively. Commonly found MPPs were in the form of fiber, fragments, foam, and pellets. Largely, MPPs were red, yellow, white, blue, and black. Among the 44 μm – 150 μm, 150 μm – 500 μm and 500 μm – 5 mm categories of size fractions, the most dominant fractions were 500 μm – 150 μm in inlet, SAT, and sludge, and 150 μm – 44 μm in the outlet sampling unit. The Guheshwori WWTP was able to remove 72.5 % of MPPs on average, that mostly occurred in the inlet. The effluent released into the river and the sludge still contained a significant number of MPPs.

Lignosulfonate-Based Carbon-Supported Pellets Catalyst to Enhance Sustainable Biofuel Production from Waste Cooking Oil.

In this study, a cost-effective and stable heterogeneous acidic carbocatalyst (CZnLS950) derived from Na-lignosulfonate (LS), a side product of the paper industry, was employed to produce hydrocarbon fuels through the pyrolysis of waste cooking oil (WCO) and crude natural-oil extracted from sunflower seeds, aligning with the principles of the circular economy. To enhance its practicality in industrial settings, the catalyst was synthesized in pellet form, enabling easy separation from the biofuel produced during the reaction. CZnLS950 exhibited remarkable catalytic efficiency in the pyrolysis of WCO, resulting in a 71 wt. % liquid biofuel yield under mild conditions. This performance is attributed to the unique synthesis procedure of acidic carbocatalyst, which utilizes LS and nano ZnO (20 nm) to create a hierarchical pore structure with acidic properties (1.1 mmol of NH3 g-1). Stability and reusability of the carbocatalyst were evaluated, and the results showed excellent stability with small catalytic deactivation (~5 wt. %) after the fourth use. Attempts at distinct catalytic mechanisms for WCO and sunflower seeds crude natural-oil pyrolysis were provided to understand the processes involved in obtaining the two different biofuels produced. Overall, this study sets the stage for exploring Lignosulfonate-based materials to achieve renewable biofuel from recycling streams.

Obtention of pelleted Kluyveromyces marxianus CIDCA 9121 with immunomodulatory properties: Selection of protective agents using Plackett–Burman and Box–Behnken experimental designs

Probiotic yeasts are increasingly used in different animal production systems. Supply of feeding in the form of pellets provides productive advantages in several systems such as poultry production or aquaculture. The inclusion of viable yeast in the pellets has to overcome heat stress during palletization and also dehydration stress. We have worked with our previously characterized probiotic strain Kluyveromyces marxianus CIDCA 9121. We evaluated the resistance of this strain to a process dehydration and extrusion after pellet generation, conditions that were not analyzed for this genus of probiotic yeast. We determined the effects of different thermoprotective agents in order to maximize yeast viability over time and preservation of its probiotic traits. To this aim we used the single factor optimization method, Plackett-Burman central compound rotating design (P–B CCRD) and response surface methodology (RSM) for optimize survival conditions in a process performed at laboratory scale. We selected a combination of sucrose, maltose and maltodextrin and we have tested two different combinations of these agents in a pilot plant extrusion/dehydration process showing that one of them could increase yeast viability. Remarkably, we could observe that immunomodulatory capacity of this strain was not affected after dehydration in the presence of the different additives tested and subsequent rehydration, being the first report showing conservation of probiotic traits of K. marxianus strain upon pelleting opening new perspectives for product development.

47 Application of a ‘high’ or ‘low’ energy matrix to a nutrient-reduced diet supplemented with a novel phytase maintained growth performance and conferred production and sustainability benefit

Abstract This study assessed the capacity of a novel consensus bacterial 6-phytase variant (PhyG) to maintain growth performance of weaned pigs when added to a nutrient-reduced mixed cereal diet with two levels of dietary net energy (NE) reduction applied. A total of 360 newly weaned pigs [Greater York × Norsvin Landrace; initial body weight (BW) 8.4 ± 0.78 kg] were assigned to 60 floor-pens and 5 dietary treatments in a randomized complete block design. There were 2 blocks (weaning group), 6 replicate pens per block, and 6 pigs/pen (3 male, 3 female), in total of 12 replications per treatment. Diets were based on wheat, corn, barley, and soybean meal and were offered to pigs in 2 phases in pelleted form (0–14 and 14–35 d post-weaning). Treatments comprised a nutritionally adequate positive control (PC) and 4 negative control (NC) diets supplemented with PhyG. The NC diets were reduced in digestible P, Ca, Na, and digestible amino acids vs. PC, according to the expected contribution of PhyG added at 1,000 phytase units (FTU)/kg (NC1) or 2,000 FTU/kg (NC2), respectively. Each NC1 and NC2 was formulated at two NE reduction levels, designated ‘low’ and ‘high’ (‘low’: -53 to -65 kcal/kg and ‘high’: -73 to -86 kcal/kg, vs. PC at 1,000 and 2,000 FTU/kg PhyG, respectively). Data were analyzed by ANOVA. Means separation was by Tukey’s HSD test. During both phases and overall (0–35 d), average BW, average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) in all PhyG-supplemented, nutrient- and NE-reduced treatments, were either not different or were improved compared with the PC. Overall ADG, ADFI, and final BW (d 35) were greater in NC2+PhyG 2,000 FTU/kg ‘low’ vs. PC (466 vs. 424 g, 9.9%, 622 vs. 567 g, 9.7%, and 24.7 vs. 23.1 kg, 6.9%, respectively; P < 0.05). Total feed costs (ingredients price, February 2023) and estimated carbon footprint (CFP; FeedPrint, WUR, The Netherland) per kilogram of BW gain (BWG) were reduced in all PhyG-supplemented, nutrient- and NE-reduced treatments vs. PC. Feed costs saving per kg BWG were greater with ‘high’ than ‘low’ NE reduction applied (-0.013 vs. -0.007 €, respectively, vs. PC) with PhyG at 1,000 FTU/kg, and it was greatest in NC2+PhyG 2,000 FTU/kg with ‘low’ NE reduction (-0.036 € vs. PC). Similarly, the CFP was reduced from 2,068 to 1,840 g CO2 eq/kg BWG (-11 %) by NC2+PhyG 2,000 FTU/kg with ‘low’ NE reduction vs. PC. In conclusion, the application of a full nutrient matrix with ‘high’ or ‘low’ NE reduction combined with supplementation of PhyG maintained growth performance of weaned pigs and reduced the total feed costs and carbon footprint per kg BW gain, lead to production and sustainability benefits.

Growth, survival, digestive activity and respiratory metabolism of Octopus maya juveniles fed with prepared diets.

The effects of two prepared feeds were tested on growth, survival, enzymatic activity, nutritive reserves in the digestive gland and oxygen consumption of Octopus maya juveniles. For the first time, a semihumid paste (HD, control) and a dry diet, in pelleted form (PD, experimental) with the same formulation were used for this species. The experiment lasted for 42 days. Results indicate that growth rates were similar for both diets (p > 0.05); however, survival (70%) was higher with the PD compared to the HD (48%) (p < 0.05). The performance index was higher for octopuses fed the PD (p < 0.05). No differences in acid proteases activity were observed. However, a higher activity of alkaline proteases in the octopuses fed the PD was observed (p < 0.05). Ingestion rate was higher for octopuses fed the PD. Routine energy inversion was similar in both treatments (p > 0.05). A greater energy inversion was observed in octopuses fed the PD, whose active metabolism was double compared to the octopuses fed the HD. Results showed that the PD promoted similar growth compared to the HD diet but favored survival, and a greater investment in the active metabolism, reflected in the apparent heat increase.

Synthesis and Characterization of Ruminant Feed Based on Palm Kernel Cake Using EM4 Fermentation Method

The purpose of this study was to determine the effect of the addition of cassava as an adhesive and the use of palm kernel meal as a concentrate and the addition of other ingredients on the physical, mechanical and chemical properties of food ingredients in the form of pellets. The animal feed was made by mixing palm kernel meal, tofu dregs, rice bran, and cassava with a percentage of (68,5 : 15,75 : 5,25 : 10,5)% and mixing molasses and EM 4 in a ration 1 : 1. The test samples were made by going through the stages of weighing, chopping, mixing, fermentation, drying, and molding into pellets. The results showed that the animal feed mixture had a specific gravity of 1.70 gr/mL, porosity of 29.05%, water absorption of 72.62%, pellet hardness of 4.85%, and brittleness with 4.92%. Animal feed pellets have a brownish color and a normal smell like the smell of animal feed pellets in general. The fermentation process was carried out for 8 days until it reached a maturity temperature of 38.44°C. Based on the proximate analysis, it was found that the animal feed contained 10.50% protein, 2.30% total fat, 22.80% crude fiber, 28.50% carbohydrate, 3.00% ash content and 32.90% moisture content. This animal feed pellet with palm kernel meal concentrate and cassava adhesive can be used as an alternative to cattle feed as an energy source feed, because it has a protein content of less than 20%.

Quantification of Irgafos P-168 and Degradative Profile in Samples of a Polypropylene/Polyethylene Composite Using Microwave, Ultrasound and Soxhlet Extraction Techniques

In polypropylene/polyethylene composite (C-PP/PE) production, stabilizing additives such as Irgafos P-168 are essential as antioxidant agents. In this study, an investigation was carried out that covers different solid–liquid extraction methods (Soxhlet, ultrasound, and microwaves); various variables were evaluated, such as temperature, extraction time, the choice of solvents, and the type of C-PP/PE used, and the gas chromatography coupled to mass spectrometry (GC-MS) technique was used to quantify the presence of Irgafos P-168 in the C-PP/PE samples. The results revealed that microwave extraction was the most effective in recovering Irgafos P-168. A recovery of 96.7% was achieved when using dichloromethane as a solvent, and 92.83% was achieved when using limonene as a solvent. The ultrasound technique recovered 91.74% using dichloromethane and 89.71% using limonene. The Soxhlet extraction method showed the lowest recovery percentages of 57.39% using dichloromethane as a solvent and 55.76% with limonene, especially when the C-PP/PE was in the form of pellets. The degradation products that obtained the highest degradation percentages were Bis (di-test-butyl phenyl) phosphate and Mono (di-test-butyl phenyl) phosphate using the microwave method with dichloromethane as a solvent and PP in film. Finally, the possible mechanisms for forming the degradation compounds of Irgafos P-168 were postulated.

Synthesis and dosimetric characterization of lithium tetraborate (Li2B4O7:Cu,Ag) thermoluminescent dosimeter with improved reproducibility and reusability

A nano-sized, tissue equivalent, polycrystalline Li2B4O7:Cu, Ag phosphor was synthesized for dosimetric applications. Repeated annealing, irradiation and readouts may alter the color and affect the sensitivity of the phosphor. One of the aims of the present work was to dope Li2B4O7 with an appropriate amount of Ag and Cu atoms in order to diminish such effects and consequently improve reproducibility and reusability of the dosimeter. For this purpose, Li2B4O7 was primarily doped with Ag ions, and the Cu atoms were introduced with minimal concetration. A sample co-doped with 0.35 wt% Ag and 0.015 wt% Cu exhibited optimum TL response. To facilitate handling of the material, the powdered sample was also reproduced in pellet form. Linear integrated TL response was observed from 1 mGy to 20 Gy. No change in material's sensitivity and color was observed after repeated annealing, irradiation and readout episodes. Response of the material was found to be reproducible with a COV between 1.7 and 2.3%. Thermal quenching effects with increasing heating rate were seen for the current material. Less than 5% fading was observed during a two month study. The obtained glow curve was very simple having a main dosimetric peak at about 200 ± 10 °C with two small shoulder peaks. Kinetic analysis of the glow curve was performed by an experimental method (Tm-Tstop) and by curve fitting approximation. Kinetic parameters (b, s and E) were found within the prescribed limits. The promising dosimetric characteristics and the optimal glow curve favours this new material for use as a field dosimeter.

Deciphering the seasonal dynamics of microplastic morphotypes and associated co-contaminants along the northwest coast of India

In the present study, the northwest coast of India, bordering the Arabian Sea, was selected to evaluate the microplastic (MP) abundance. This is the first study to emphasize the effects of different seasons on MP distribution. The collected MPs were dried, segregated, and evaluated based on their morphotype, size, color, and polymer type. A total of 1756.6, 7326.6, and 202 particles/kg of sand were estimated in the pre-monsoon, monsoon and post-monsoon seasons, respectively, with a dominance of polypropylene (PP) type of plastic in the pre-monsoon and high-density polyethylene (HDPE) in monsoon and post-monsoon seasons. HDPE and PP collected MPs during the monsoon season were further characterized for associated contaminants. Metal absorbance was detected using SEM-EDX mapping and ICP-MS. The presence of organic compounds (OCs) was analyzed using GC-MS. MPs exhibit distinct associations with metals, among which the HDPE pellet morphotype exhibits a higher range of metal adsorption. Total 61 different OCs were associated with MPs. The HDPE pellets contained the highest amounts of hydrophobic organic compounds. PP pellets were found to contain triglycerides, fatty aldehydes, and alkaloids, along with HOCs. Among morphotypes, pellet forms of MPs were found to adsorb more contaminants. These co-contaminants infiltrate the study area through sewage runoff and shoreline debris deposition, subsequently interacting with MPs. Furthermore, the MP diversity was studied by employing the MP diversity integrated index, which suggests that most of the MP diversity was observed in the pre-monsoon period. The pollution load index employed an MP risk assessment, which presented a low degree of MP contamination. In contrast, the polymer hazard index was calculated as 21650.3 in post-monsoon, placing the area under the extreme danger category. It is evident from the data that the types of MP is more important than their number. Thus, MP morphotypes have importance in the adsorption of co-contaminants.

Exploring novel quorum quenching strain: Enhanced disrupting autoinducer-2 bacterial communication to combat biofouling in membrane bioreactor for wastewater treatment

Prior investigations concerning quorum quenching (QQ), which hinders quorum sensing (QS) in microbial communication, concentrated predominantly on N-acylhomoserine lactones (AHLs), which are communicated merely by gram-negative bacteria. However, to combat biofouling more effectively, particularly biofilm-related issues, it is important to explore the inhibition of autoinducer-2 (AI-2), a universal signal (interspecies communication) employed by both gram-negative and gram-positive bacteria. This study aimed to isolate and identify novel strains capable of more effectively inhibiting AI-2 signaling for use in engineering fields. The results revealed that the newly isolated strain Pantoea sp. PL-1 demonstrated remarkable efficacy in attenuating AI-2 signals, leading to a substantial reduction in the AI-2 precursor (S)-4,5-dihydroxy-2,3-pentandione (DPD) in both its pellet (with a DPD removal efficiency of 90.8 % after 180 min, with a rate constant of k = 0.8 h−1) and supernatant form (66.9 %, k = 0.37 h−1). Interestingly, the findings indicate that strain DKY-1, a strain previously reported to exhibit AI-2 QQ activity, only reduced DPD levels in its supernatant form (extracellular activity), while both the pellet and the supernatant of strain PL-1 demonstrated AI-2 QQ activity, indicating both intracellular and extracellular QQ activity. Adding the PL-1 supernatant to co-cultures of two AI-2 QS strains, E. coli K12 and P. mirabilis, successfully reduced DPD by 40 % and 70 %, respectively, without impeding their growth. Additionally, when assessing the impact of PL-1 to minimize membrane biofouling in a membrane bioreactor, it demonstrated superior performance compared to the DKY-1 strain, with a 40 % improvement. The AI-2 QQ compound of PL-1 has been identified as highly hydrophilic and low-molecular-weight, and its exact properties have not yet been fully elucidated. Therefore, uncovering its identity may be an important goal for future research.

Comparing Wood Chip Quality from Different Sources across the Southeastern United States

Abstract Global use of renewable energy has risen over the past few decades because of international energy policy changes, new legislation, and an effort to create a more sustainable energy source for a rapidly growing global population. One of these renewable energy sources is biofuels, specifically in the form of wood pellets created from wood chips. Wood pellet manufacturers in the southeastern United States must adhere to rigid quality-control standards of the European markets where their pellets are shipped and consumed. Thus, there is a need to improve our understanding of the factors that influence the quality of the source materials. Higher heating value (HHV), moisture content (MC), ash content, elemental composition, and size stratification are all important quality factors to consider when analyzing wood chips. Variations in these quality factors can cause longer drying times, blockages in feed systems, excess waste, and fluctuations in energy output. The objective of this study was to quantify these quality factors across multiple sources of wood chips. Softwood and hardwood in-woods chip samples as well as softwood mill residual chips were collected from thinnings and clear-cuts across the southeastern United States. Softwoods had a greater HHV and MC than hardwood chips, with 18,949 kJ/kg and 18,242 kJ/kg, respectively. Softwood thinnings had the highest HHV and lowest ash content. However, only 4 of the 60 samples analyzed were from softwood thinnings. This study provides an overview of the variation in wood chip quality across the southeastern United States.

Synthesis of L-Lactide from Lactic Acid and Production of PLA Pellets: Full-Cycle Laboratory-Scale Technology.

Lactide is one of the most popular and promising monomers for the synthesis of biocompatible and biodegradable polylactide and its copolymers. The goal of this work was to carry out a full cycle of polylactide production from lactic acid. Process conditions and ratios of reagents were optimized, and the key properties of the synthesized polymers were investigated. The influence of synthesis conditions and the molecular weight of lactic acid oligomers on the yield of lactide was studied. Lactide polymerization was first carried out in a 500 mL flask and then scaled up and carried out in a 2000 mL laboratory reactor setup with a combined extruder. Initially, the lactic acid solution was concentrated to remove free water; then, the oligomerization and synthesis of lactide were carried out in one flask in the presence of various concentrations of tin octoate catalyst at temperatures from 150 to 210 °C. The yield of lactide was 67-69%. The resulting raw lactide was purified by recrystallization in solvents. The yield of lactide after recrystallization in butyl acetate (selected as the optimal solvent for laboratory purification) was 41.4%. Further, the polymerization of lactide was carried out in a reactor unit at a tin octoate catalyst concentration of 500 ppm. Conversion was 95%; Mw = 228 kDa; and PDI = 1.94. The resulting products were studied by differential scanning calorimetry, NMR spectroscopy and gel permeation chromatography. The resulting polylactide in the form of pellets was obtained using an extruder and a pelletizer.

Moisture Optimization and Energy Saving Effects of Combined Organic Acid and Surfactant Inclusion in Pelleted Feed Production

The purpose of the present study was to determine the effects of inclusion of an organic acid and surfactant (OS) combination on moisture optimization and energy sparing in the production of pelleted compound feeds for dairy and beef cattle. The trial was carried out in two independent private commercial feed factories (factories A and B) producing cattle feed in pellet form. Each factory produced 21 tons of commercial cattle feed (7 batches; 3 tons per batch); factory A, a dairy feed containing 2620 kcal/kg metabolizable energy (ME) with 18.90% crude protein (CP); and factory B, a fattening feed containing 2550 kcal/kg ME with 13.00% CP. Batches for the treatment groups were prepared by adding 0.5, 1.0 and 1.5 kg/ton of OS (Fylax flow) respectively to these basal feeds in the mixer. The moisture retention capacity during pelleting process of all three OS supplemented feeds increased in comparison to the basal feed, whilst moisture content of the finished feeds and energy consumed for production decreased significantly. It was observed that increasing the OS supplementation to 1.5 kg could further increase the moisture retention capacity and moisture content in pellet production compared to the feeds supplemented with 0.5 and 1.0 kg OS, due to the lower power rating of the equipment. It has thus been concluded that adding 0.5, 1.0 and 1.5 kg of OS to commercial compound feeds for dairy resulted in a profitable production with good moisture optimization and energy savings during pelleting.

Experimental Characterization of Screw-Extruded Carbon Fibre-Reinforced Polyamide: Design for Aeronautical Mould Preforms with Multiphysics Computational Guidance

In this research work, the suitability of short carbon fibre-reinforced polyamide 6 in pellet form for printing an aeronautical mould preform with specific thermomechanical requirements is investigated. This research study is based on an extensive experimental characterization campaign, in which the principal mechanical properties of the printed material are determined. Furthermore, the temperature dependency of the material properties is characterized by testing samples at different temperatures for bead printing and stacking directions. Additionally, the thermal properties of the material are characterized, including the coefficient of thermal expansion. Moreover, the influence of printing machine parameters is evaluated by comparing the obtained tensile moduli and strengths of several manufactured samples at room temperature. The results show that the moduli and strengths can vary from 78% to 112% and from 55% to 87%, respectively. Based on a real case study of its aeronautical use and on the experimental data from the characterization stage, a new mould design is iteratively developed with multiphysics computational guidance, considering 3D printing features and limitations. Specific design drivers are identified from the observed material’s thermomechanical performance. The designed mould, whose mass is reduced around 90% in comparison to that of the original invar design, is numerically proven to fulfil thermal and mechanical requirements with a high performance.

Dynamic Optimization of Lactic Acid Production from Grape Stalk Solid-State Fermentation with Rhizopus oryzae Applying a Variable Temperature Profile

Lactic acid is widely used in the food industry. It can be produced via chemical synthesis or biotechnological pathways by using renewable resources as substrates. The main challenge of sustainable production lies in reaching productivities and yields that allow for their industrial production. In this case, the application of process engineering becomes a crucial tool to improve the performance of bioprocesses. In this work, we performed the solid-state fermentation of grape stalk using Rhizopus oryzae NCIM 1299 to obtain lactic acid, employing three different temperatures (22, 35, and 40 °C) and a relative humidity of 50%. The Logistic and First-Order Plus Dead Time models were adjusted for fungal biomass growth, and the Luedeking and Piret with Delay Time model was used for lactic acid production, obtaining higher R2 values in all cases. At 40 °C, it was observed that Rhizopus oryzae grew in pellet form, resulting in an increase in lactic acid productivity. In this context, the effect of temperature on the kinetic parameters was evaluated with a polynomial correlation. Finally, using this correlation, a smooth and continuous optimal temperature profile was obtained by a dynamic optimization method, improving the final lactic acid concentration by 53%.

Age-Related Variations in the Apparent Metabolizable Energy of Meat and Bone Meal for Broilers.

The influence of broiler age on the apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) of meat and bone meal (MBM) was investigated. A corn-soy basal diet and an experimental diet wherein 300 g/kg of the basal diet was replaced (w/w) with MBM were developed. The diets, in pellet form, were fed to six replicate cages across six age groups, namely d 1 to 7, 8 to 14, 15 to 21, 22 to 28, 29 to 35 or 36 to 42 d post-hatch. Birds were fed either a starter diet from d 1-21 or a finisher diet from d 22-35. Basal and experimental diets were introduced on d 1, 8, 15, 22, 29 and 36 with 10 (d 1-7), 8 (d 8-14) and 6 (d 15-42) birds per replicate. Total collection of excreta was carried out during the last 4 d of each age period. A linear decrease (p < 0.001) in the retention of dry matter and nitrogen was observed with advancing age. The AMEn of MBM showed a linear increase (p < 0.05), rising from 12.56 MJ/kg during d 1-7 to 13.90 MJ/kg during d 29-35, followed by a decline to 13.41 MJ/kg during d 36-42. The current findings showed that the energy utilization of MBM increased with the advancing age of broilers. Age-dependent AMEn values of MBM may need to be considered when MBM is included in feed formulations.

Design, development and scale up studies of metformin hydrochloride multiparticulate system: A promising alternative to large size tablet

Metformin hydrochloride (MH) is prescribed for treating diabetes mellitus. The most popular dosage form, viz., tablets has a bulky nature as MH dose ranges up to 1000 mg leading to large size. In geriatric or dysphagic patients, due to altered physiology, swallowing such bulky formulation is troublesome. Thus, this study aims towards formulation of palatable multiparticulate system in the form of pellets, which are presented in unit sachets, in the dose of 1000 mg and 500 mg. These pellets in sachets can be substituted for bulky tablets. The main challenge of this study was to mask the bitter taste of MH, which could be achieved by applying a coat of sweetener to the pellets. The formulation was optimized using 23 factorial design. Povidone as a binder, quantity of sucralose in coating and amount of coating over pellets were considered as independent factors at two levels. The pellets were evaluated for surface texture, bulk density, sweetness, dissolution and assay. Their manufacturing process was scaled up at 50X level compared to the laboratory batch size. The stability studies were conducted as per the ICH guidelines using 3 ply sachets (12 μm polyester/9 μm aluminium/63 μm polyethylene). The optimized formulation was free flowing having a sweet and pleasant taste, exhibited acceptable compliance with the quality standards. The formulations were found stable for 6 months at accelerated conditions and 12 months at the room temperature during stability study. Hence, the present work can provide an alternate option for better patient compliance.

Effect of continuous or intermittent feeding of ergot contaminated grain in a mash or pelleted form on the performance and health of feedlot beef steers.

This study evaluated the effect of feeding ergot contaminated grain continuously or intermittently through backgrounding (BG) and finishing (FN) in a mash or pelleted supplement on the growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Sixty black Angus steers (300 ± 29.4kg BW) were used in a complete randomized 238-d study. Steers were stratified by weight and randomly assigned to four different diets (15 steers/treatment) and individually housed. Treatments included: (1) control [CON; no added ergot alkaloids (EA)], (2) continuous ergot mash (CEM; fed continuously at 2mg total EA/kg of DM), (3) intermittent ergot mash (IEM; fed at 2mg total EA/kg of DM, during the first week of each 21-d period and CON for the remaining 2wk, this feeding pattern was repeated in each period), and (4) intermittent ergot pellet (IEP; fed at 2mg of total EA/kg of DM as a pellet during the first week of each 21-d period and CON for the remaining 2wk as described for IEM). Steers were fed barley based BG diets containing 40% concentrate:60% silage (DM basis) for 84 d (four 21-d periods), transitioned over 28 d (no ergot fed) to an FN diet (90% concentrate:10% silage DM basis) and fed for 126 d (six 21-d periods) before slaughter. In the BG phase, steer DMI (P < 0.01, 7.45 vs. 8.05kg/d) and ADG (P < 0.01) were reduced for all EA diets compared to CON. The CEM fed steers had lower ADG (P < 0.01, 0.735 vs. 0.980kg) and shrunk final BW (P < 0.01, 350 vs. 366kg) than CON. CEM had lower gain:feed (P < 0.07, 0.130 vs. 0.142) than CON. In the FN phase, steer DMI (P < 0.01, 9.95 vs. 11.05kg/d) and ADG (P = 0.04) were also decreased for all EA fed steers compared to CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2kg), final BW (P = 0.03, 617.9 vs. 662.2kg), and carcass weight (P = 0.06) decreased for all EA fed steers compared to CON. The percentage of AAA carcasses decreased for all EA fed steers (P < 0.01, 46.7 vs. 93.3%) compared to CON. EA fed steers had increased rectal temperatures (P < 0.01, 39.8 vs. 39.4 °C) compared to CON. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters during BG or FN. Continuously or intermittently feeding ergot contaminated diets (2mg total EA/kg of DM) significantly reduced intake, growth performance, and carcass weight, with minimal impact on blood parameters in feedlot steers. Pelleting was not an effective method of reducing ergot toxicity.