Forensic Anthropology Research Articles

The Boundary of Artificial Intelligence in Forensic Science

Artificial intelligence (AI) is poised to revolutionize forensic science by enhancing evidence analysis and aiding in crime-solving. This paper explores AI applications in forensic fields, focusing on DNA mixture analysis, the cause of death diagnosis, and the challenges AI faces in forensic science. It addresses the limitations of AI in forensic science, emphasizing the necessity for transparent AI systems used ethically. Forensic errors are often not due to misclassification by forensic scientists but stem from incompetence, fraud, weak scientific foundations, or organizational deficiencies, resulting in wrongful convictions. Systemic issues like dependence on presumptive tests and inadequate defense contribute to errors. Inconsistencies plague various domains of forensic science, including fingerprint and DNA analysis. Establishing standardized analytical procedures can enhance consistency and credibility in forensic science. AI applications in forensic anthropology, pathology, genetics, and statistics are explored, with a focus on their potential and challenges.

Sex Estimation Using Humerus Volume of CT Images in Korean Population: A Metric Study

This study aimed to explore the use of three-dimensional reconstructed computed tomography images of the adult humerus for forensic anthropological sex estimation in the Korean population. In forensic anthropology, the skull and pelvic bone show high accuracy in sex estimation; however, when they are impaired, other bones should be available for sex estimation. Six hundred images of the right and left humerus pair were used. A logistic regression analysis was performed by measuring the volumes of 500 images and the remaining 100 images were used in the metric sex estimation. Both the logistic regression and metric measurements were performed separately on three regions of the right and left humerus: the head, mid-shaft, and digital regions, The accuracy of sex estimation in the right humerus was 93% (91% for men and 96% for women), 92% (93% for men and 91% for women), and 87% (85% for men and 89% for women) for the head, mid-shaft, and digital regions, respectively. Sex estimation accuracy in the left humerus was 92% (89% for men and 96% for women), 93% (96% for men and 89% for women), and 91% (91% for men and 91% for women) for the head, mid-shaft, and digital regions, respectively. These results contribute to high accuracy and reliability in sex estimation using three-dimensional images of the humerus in Koreans, whereas the two-dimensional metric method provided limited information on bone measurements.

Pioneers of Color

Popular renditions on the history of forensic anthropology have traced the discipline’s roots back to early European anatomy and nineteenth to twentieth-century American research and applications to the legal system, often highlighting the works of several recurring figures. These forebearers are overwhelmingly composed of white men to the exclusion of, as we argue here, pioneers of color. As a counter to prevailing Eurocentric narratives, we present the biographies of diverse contemporaries who were equally foundational to the field, including Black Americans, immigrants, and luminaries outside of the Western world. Common themes among their experiences involved discrimination, a lack of opportunities and recognition, and a biocultural and humanistic praxis that demonstrate modern discourses within the forensic anthropology community are not novel. Ultimately, this work shows that the historical foundations of forensic anthropology, in both the United States and globally, include a far more diverse cast of pioneers than what the prevailing literature suggests and should serve as a springboard from which our discipline can grow, both in its past and in its future.

Teaching Virtual Forensic Anthropology Labs: Methods and Reflections

Development of virtual labs for Forensic Anthropology was complicated by the notion that the skeleton cannot be learned without physical manipulation. This was addressed by using free programs to teach using 3D models of bone. Successes and shortcomings are discussed based on student and educator feedback. Integration of 3D models in teaching is plausible as it reduces deterioration of specimens and increases accessibility of the lab, however, the ethics of digital archaeology, including curation of human skeletal models, is an unsolved challenge. Overall, although 3D modelling cannot replace hands-on learning, teaching virtually can indeed ensure high-quality instruction is delivered.

Racial Bias in Forensic Decision Making. Comment on Yim, A.-D.; Passalacqua, N.V. A Systematic Review and Meta-Analysis of the Effects of Race in the Criminal Justice System with Respect to Forensic Science Decision Making: Implications for Forensic Anthropology. Humans 2023, 3, 203–218

A “Systematic Review and Meta-Analysis of the Effects of Race in the Criminal Justice System” [...]

Morphological Observation of the Alar Region between the Piriform Aperture and the Alar Soft Tissue for Facial Approximation (Reconstruction)

Prediction methods for regions not lined by facial bones are important elements of forensic anthropology, including forensic facial approximation. The alar region can be predicted by measuring the distances from the piriform margin and multiples of the piriform width. However, studies investigating this region are limited, and some only focus on one side of the alar region. Hence, we aimed to measure the bony points/landmarks to predict the alar region.Post-mortem computed tomography images of the samples from 180 Japanese individuals were obtained and analyzed. The images were processed using Osirix (Pixmeo). The coordinates recorded from all samples were calculated, and the values obtained were stratified by sex, laterality, age, body mass index, and cephalic index. The distances between the alar and the five piriform points were measured. The mean, standard deviation, and confidence interval of all samples were calculated, and the sex-related differences were determined. We evaluated the correlation between alares and other boney landmarks and points. Our findings were compared with those of previous studies. We observed significant sex differences (p < 0.01) and asymmetry (p < 0.01) in laterality in this region; hence, asymmetry was assessed based on these results. Compared with the results of previous studies, the obtained measurements were larger than those of a previous Asian study, suggesting that laterality may influence the accuracy of prediction methods for regions not lined by hard tissues.

Testing the accuracy of the DRNNAGE software for age estimation in a modern Greek sample

Estimation of age-at-death from human skeletal remains is fundamental in forensic anthropology as part of the construction of the biological profile of the individual under study. At the same time, skeletal age-at-death estimation in adults is problematic due to the disparity between chronological and biological age, the important inter-individual variability at the rate of skeletal aging, and inherent biases in the available methodologies (e.g., age mimicry). A recent paper proposed a method for skeletal age-at-death estimation based on multiple anatomical traits and machine learning. A software was also created, DRNNAGE, for the easy implementation of this method. The authors of that study supported that their methods have very high repeatability and reproducibility, and the mean absolute error of the age estimation was ~6 years across the entire adult age span, which is particularly high and promising. This paper tests the proposed methodology on a modern documented Greek sample of 219 adult individuals from the Athens Collection, with age-at-death from 19 to 99 years old. The sample was split into males and females as well as into individuals under and over 50 years old. We also divided the sample in 10-year intervals. First, intra- and inter-observer error was estimated in order to assess repeatability and reproducibility of the variables employed for age-at-death estimation. Then, the validity (correct classification performance) of DRNNAGE for each anatomical region individually, as well as all combined, was evaluated on each demographic separately and on the pooled sample. According to the results, some of the variables showed very low repeatability and reproducibility, thus their use should be cautious. The DRNNAGE software showed overall highly accurate age-at-death estimates for individuals older than 50 years, but poor on younger adults, with only exception the cranial sutures, which performed surprisingly well for all age groups. Overall, these results support the importance of cross-validation and the use of population-specific methods in forensic anthropology.

Violence against children: A review of cases at a forensic anthropology unit, Johannesburg, South Africa

South Africa is experiencing a scourge of violence against women and children, with alarming levels of violence, and as such, juvenile cases are sometimes encountered in a forensic anthropological setting. The aim of this research was to assess the cases of juveniles (under 20 years) presenting at the Human Variation and Identification Research Unit (HVIRU) for a 6-year period (2016–2022), in order to assess patterns and types of cases referred. A total of 19 cases were assessed, of which 10 were 16–20 years old, 3 between the ages of 5 and 15 years and 6 less than 5 years old at the time of death. Of the 14 children with known sex, 12 were female with features suggesting that they fell prey to sexual violence. Many of the individuals were either known (and referred for trauma analysis) or identified following investigation. Of the 19 juveniles, 11 (58 %) had evidence of perimortem trauma (sharp and/or blunt), attesting to the violent nature of their deaths. Two cases had both sharp and blunt force trauma, of which one is a possible case of dismemberment. The remains of two individuals showed signs of perimortem burning, which may or may not be related to the cause of death. Three individuals had signs of antemortem (healed) trauma, which may suggest a longer period of abuse. Five of the individuals showed signs of disease – two had cribra orbitalia, while two others had various porous lesions indicative of chronic disease or malnutrition. One individual had advanced osteomyelitis, suggesting a natural cause of death. Some of these cases had unusual trauma and pathology, highlighting the contribution of forensic anthropologists.

Luminol and the postmortem interval estimation — influence of taphonomic factors

The estimation of the postmortem interval (PMI) is one of the key challenges for forensic anthropologists. Although there are several methods referenced for this purpose, none is sufficiently effective. One of the main reasons justifying the complexity of this task is the influence of several taphonomic factors.The study of the Luminol technique has stood out as a promising method for estimating PMI, complementing the existing methods, since it is an economic, easy and reproducible method that operates as a presumptive test. However, it is not known which taphonomic factors can influence the results obtained by this technique.The aim of this study is to test the influence of taphonomic factors, such as temperature, humidity, soil type and pH, on the estimation of the PMI by the Luminol technique.In order to test the influence of the referred factors, a sample consisting of 30 clavicles, with known and similar PMI, collected from autopsies, was distributed as evenly as possible by six vases and buried with different decomposition conditions for a period of 12 months. After the exhumation and sample preparation, the Luminol technique was applied.It was possible to clearly observe differences in the results. Thus, according to our research, it is possible to conclude that the results obtained by the application of Luminol are influenced by taphonomic factors. Therefore, the context in which a body is found should always be considered for applying this technique.

Bones have other stories to tell. Review of: The marginalized in death: A forensic anthropology of intersectional identity in the modern era. Edited by Jennifer F. Byrnes and Iván Sandoval‐Cervantes

Bones have other stories to tell. Review of: The marginalized in death: A forensic anthropology of intersectional identity in the modern era. Edited by Jennifer F. Byrnes and Iván Sandoval‐Cervantes

Awareness of forensic anthropology in Switzerland: a survey among forensic practitioners, police, and prosecutors.

Forensic anthropology (FA) as a specialized discipline has been practised in multi-lingual Switzerland for over a decade. A variety of expertise regarding osteological assessments as well as facial image comparison (FIC) is provided by different centres. Nevertheless, information is lacking about the awareness of FA and its benefits for forensic investigations among forensic stakeholders. Therefore, a survey was sent to Swiss anthropologists (AN) and related professions (police officers, prosecutors, and forensic pathologists) to assess three main aspects: (1) the experience of working (biological/forensic) anthropologists within FA; (2) how FA is perceived by other professions within the legal system; and (3) identifygaps (if any) in understanding of FA with the aim to suggest avenues for improvement if necessary. The results show that awareness of FA varies by occupation and cantonal regions. In areas where close collaborations betweenforensic anthropologists (FAs) and other stakeholders have been formally established, be it with focus on osteological analyses or FIC, the awareness of FA competencies was superior to areas where this was not the case. An overwhelming majority of forensic actors expressed interest in continuing education related to the role of FA. These findings indicate that facilitation of communication and collaboration leads to improvement in the awareness of the competencies of FAs and their contribution to forensic investigations.

Transcending time: the forensic anthropological case study of three unidentified transgender women in Italy in the early 1990s.

In forensic anthropology, the estimation of skeletal sex results in a dichotomous outcome: male or female. This poses challenges when dealing with transgender individuals. Italy has the highest rate per capita of transgender murders, making their identification a compelling issue. This study presents three cases of unidentified skeletal remains of transgender women (MtF) that underwent autopsies in the 1990s at the Institute of Legal Medicine of Milan. The transgender status of the individuals was determined from the autopsy records which indicated the co-existence of breast implants and male genitalia. The biological profile was created by estimating sex, age, ancestry, and stature, and the bones were investigated looking for evidence of Facial Feminization Surgery (FFS). Anthropological estimations revealed that the skeletons belong to male, adults, European individuals, between 164 and 184cm. Fragmented gelatinous masses were found in association with two skeletons, which were interpreted as remnants of breast implants. In two cases, signs of remodeling of the cortical surface were observed on the zygomatic bones, although the bone marks observed here were not specific enough to link them to FFS. Despite some limitations, this study highlights the need for greater awareness among practitioners about this limitedly addressed issue, advocating for a more inclusive forensic anthropology that strives to improve methods and interpretation of evidence for the identification of transgender individuals.

Maxillary sinus in gender determination: a morphometric analysis using cone beam computed tomography.

Gender determination of skeletal remains is an important forensic procedure in the identification process. Maxillary sinus remains intact even when the skull and other bones may be badly disfigured. Cone beam computed tomography (CBCT) can provide precise information about complex anatomical structures, as it is characterized by rapid volumetric image acquisition with high resolution. The present study aimed to assess the accuracy of maxillary sinus measurements using cone beam computed tomography in gender determination and to develop a formula using discriminate function analysis. Bilateral maxillary sinus images (left and right) were acquired for 200 patients (100 females and 100 males) and different parameters (width, length, height, area, perimeter, and volume) were measured and evaluated. Mean and standard deviation of both maxillary sinuses measurements were calculated and compared. The data was subjected to discriminative statistical analysis and analyzed using an unpaired t-test. The difference between all these variables was statistically significant between males and females. Based on discriminant analysis, the most pronounced variable in the differentiation of gender groups was maxillary sinus height. Gender assessment was established correctly with an accuracy of 75% for females and 64% for males with an overall accuracy of 69.5%. Adding other independent variables to the model did not result in an improvement in overall accuracy. Cone beam computed tomography measurement of maxillary sinus can be used as an aid in forensic anthropology for gender determination.

Ancestry estimation in forensic anthropology: accuracy of the AncesTrees software in a Brazilian sample.

Ancestry estimation methods are seldom validated in Brazil.AncesTrees performed poorly on our sample, with a maximum accuracy of 70%.Brazil's highly mixed population hinders ancestry estimation.Mixed individuals (pardos) are predominantly classified as Europeans.The insertion of Brazilian metric data into the AncesTrees database would produce better results.

Book Review: Mildred Trotter and the Invisible Histories of Physical and Forensic Anthropology

A Review of Mildred Trotter and the Invisible Histories of Physical and Forensic Anthropology, Emily K. Wilson, ISBN 9781032180892, CRC Press, 2022.

Database of segmentations and surface models of bones of the entire lower body created from cadaver CT scans

The range of applications of digital surface models of the bones in science and industry is wide. Three-dimensional reconstructions of bones are used in biomechanics, biomedical engineering, medical image processing, orthopedics, traumatology, radiology, patient education, anatomy, anthropometry, forensic anthropology, ergonomics, usability and human factors engineering, or accident and injury analysis and prevention. No open access database or repository of skeletal surface models of the full lower extremities exists. Therefore, the objective of this publication was to provide access to consistent complete bone models of the pelvis and lower limbs of multiple subjects, including biometric data. Segmentations and surface models of the bones of the lower extremities of more than twenty subjects were created from open access postmortem whole-body computed tomography scans. The database provides a broad range of applications by giving access to the data of the complete process chain, from the raw medical imaging data through the segmentations to the surface models.

Investigating identification disparities in forensic anthropology casework.

Forensic anthropology is shifting to reflect on the impact of its practices within the criminal justice context in important ways. Here, we contribute to this essential work by examining how decedent demographics as well as estimations of biological profile components are related to identification trends in forensic anthropology cases. The study uses data from more than 1,200 identified and unidentified forensic anthropology cases from three agencies (together representing a nation-wide sample). We found the following: i) multivariate analyses indicated that decedent sex, age, and race and/or ethnicity are not related to case identification rates in the pooled United States sample, ii) when identification rate differences do occur, they appear to be smaller effects, more agency-specific, and/or related to the context of a particular agency, iii) for the agency-specific sample with available data, there was no consistent evidence for a discrepancy in the duration of an identification investigation based on a decedent's sex, age, or race and/or ethnicity, iv) forensic anthropological estimations of sex, age, and ancestry can improve the odds of identification for decedents, although these are small effects, and v) reporting an ancestry estimation does not appear to impact decedent race representation among resolved unidentified person cases. Although previous studies have identified demographic discrepancies in other areas of the criminal justice system, the results presented here suggest that decedent demographic estimation practices by forensic anthropologists in general do not appear to be related to discrepancies in identification trends, but more research is needed to examine whether these findings hold. Contextual factors and practices specific to each investigative agency likely contribute to identification trends.

Blunt weapons in the roman imperial army. A multidisciplinary approach to the clava from experimental archaeology to forensic anthropology

The wooden club (clava) is rarely associated with the image of Roman soldiers, perhaps because it was considered an unconventional weapon with ethnic attributes and/or specific combat roles. Nonetheless, it is attested in the archaeological record and some ancient texts. The panoply and the military career of the soldiers represented on the tombstones of Catavignus son of Ivomagus (1st century AD) and Marcus Aurelius Alexis (early 3rd century AD) offered us the possibility to reconsider the use of the club as an offensive weapon within the Imperial Roman army. The authors made a replica of the clava using historical tools and manufacturing techniques. A series of tests have then been performed to investigate the use of the club in combat. The possible traumas on human targets were also evaluated with a forensic anthropological approach. The results suggest the essential role of flexion and rotation of the kinetic chain «hip/shoulder/ arm» in wielding the clava. This movement requires a much more mobile combat style that can prove very effective against heavily armoured targets due to the weapon's high-impact force and a more extended reach than more conventional Roman sidearms.

Evaluation of the reliability and accuracy of femur measurements acquired from computed tomography images

The biological profile holds notable significance within forensic assessments, as it plays a crucial role in determining biological identity. Moreover, it possesses substantial potential for applications in fields such as medical legal cases and forensic anthropology. Recent advancements in technology, specifically in the field of computed tomography, have enabled the accurate acquisition of detailed anatomical data from CT scans present in extensive medical repositories. The validity of new methods developed through the application of these techniques should therefore be analyzed. The primary intent of this research was to investigate the measurement accuracy obtained from CT-generated 3D femur models. To investigate the accuracy and reliability of measurements obtained from CT-generated 3D femur models, 3 different studies were conducted. A dataset comprising fifteen femurs was employed for analysis and measurement purposes. The obtained images were subsequently compared to twelve measurements acquired from the dry femora, enabling an assessment of the reliability and accuracy of both measurement protocols. To investigate the effect of CT parameters and soft tissue, 4 femurs were used. The analysis of twelve femur measurements obtained from the CT images processed with OsiriX software was carried out using Excel software packages and SPSS 24.0. From the results of this study, it can be observed that there is no discernible pattern regarding the reliability of image acquisition in any particular way. This implies that both dry femur bone and 3D virtual femur models can be used interchangeably for the 12 metric measurements used in this study, and at the same time, different scanning parameters or soft tissue influence for these measurements do not make a statistically significant difference.

Age estimation in the combined long bones and ribs by histomorphometry: Past, present, and future.

Numerous age estimation methods in unidentified bone have been a long time developing for application in forensic anthropology. The histomorphometric technique is one of the alternative methods that relied upon the evaluation of the cortical bone microstructure over the lifespan as a result of the remodeling process in bone. Remodeling is a sophisticated event occurring from the coupled function of bone formation and resorption cells for maintaining mineral homeostasis and repairment of microdamage in bone tissue. Products derived from remodeling are primary changes in the osteon or haversian system in various regions in the cortical bone, including periosteum, endosteum, and trabecular bone. Throughout life, bone remodeling rate with osteon alteration can be predictable. In the forensic field, histological methods are getting more attention due to the unavailability of macroscopic methods. Histomorphometry approach can be accomplished in fragmentary or incomplete bone remains indicating the limited use of gross morphological methods. In addition, the microscopic methods can aid to increase the more accuracy of analyses and diminish the biased subjective assessment for determining age. Most histomorphometry method utilizes a cross-section of the midshaft of the long bones including the mandible, rib, and clavicle. This review provides the basic knowledge of bone biology and anatomy, several age-estimating methods of histology, and crucial factors for age methods. Studies regarding overall age determination methods from the past until now contribute to obtaining more benefits for developing methods of histomorphometry using human bone in forensic identification.