Abstract Fruit and vegetable growers in the US face tradeoffs and synergies between on-farm conservation and pre-harvest food safety as a result of economic considerations, regulatory concerns, and external pressure from other stakeholders. However, detailed data on the frequency and extent of these tradeoffs across US regions remain sparse. We designed and implemented a national grower survey for the 2018 crop year to address this gap. Based on 209 responses, we examined usage of pre-harvest food safety and conservation practices with a particular emphasis on managing animal intrusion into growing areas and maintaining wildlife habitat. We also analyzed associations between farm characteristics and the probability that growers used different on-farm food safety and conservation practices. We did not find a simple biophysical or socio-economic explanation for why some farms adopted specific practices over others. Instead, our findings suggest that the adoption of particular food safety practices is influenced by a complex assemblage of factors that include environmental context, supply chain pressures, cost considerations, and growers' perceptions of risk. A better understanding of the diverse tradeoffs and synergies that US produce growers face between on-farm conservation and pre-harvest food safety is critical for effective policy design.