Nutrient Requirements Research Articles

Analysis of the Application Effectiveness of the Pregnancy Dietary Compliance Index Scoring Tool

This study aims to conduct a dietary index score assessment for individuals with high-risk factors for gestational hyperglycemia (such as overweight, obesity, family history of diabetes, history of macrosomia, and gestational diabetes mellitus), starting from early pregnancy, and to provide personalized nutrition management based on the score to reduce pregnancy complications and adverse pregnancy outcomes. A total of 194 pregnant women who underwent routine prenatal examinations at our hospital from September 2022 to September 2023 were selected. They were randomly divided into a study group and a control group, with 97 cases in each group. The control group received simple nutritional guidance, mainly focusing on the requirements for various nutrients and nutritional supplements during each trimester. The study group received nutrition guidance based on the dietary index score, as well as personalized guidance on health education, weight management, pregnancy exercise, and lifestyle. The incidence of gestational diabetes mellitus in the intervention group was significantly lower than that in the control group, and the difference was statistically significant (P < 0.05). The incidence of macrosomia was 1.03% in the intervention group and 8.25% in the control group, with a statistically significant difference (P < 0.05). The incidence of small-for-gestational-age infants was 2.06% in the intervention group and 10.31% in the control group, with a statistically significant difference (P < 0.05). For pregnant women with high-risk factors, early pregnancy nutrition management based on the dietary compliance index score can quickly screen for nutritional risks and provide targeted personalized nutrition management, thereby reducing the risk of pregnancy complications and adverse pregnancy outcomes and promoting the health of mothers and infants during pregnancy.

Effect of Moringa, Subabul and Guava Leaves Either Sole or in Combination on Nutrient Utilization, Nitrogen Balance of Ram Lambs

Background: The use of alternate feed resources like tree leaves is key element in addressing the problems derived from climate change and food shortages that demand establishment of sustainable feeding system for ruminants. Methods: A study was conducted on 36 Nellore cross ram lambs (6-7 months; 18.48±0.29 kg BW) randomly allotted to six dietary treatments to evaluate the effect of 20% dietary inclusion of moringa (MOR) subabul (SBL) and guava (GVL) leaves either sole or in combination (MS and MG mixture incorporated at 60:40 and 40:60, respectively) in concentrate mixture on nutrient utilization, plane of nutrition and nitrogen balance. The control group concentrate mixture contained maize, de-oiled rice bran, cotton seed cake and soybean meal as major ingredients and in the 5 experimental concentrate mixtures, de-oiled rice bran and soybean meal were partially replaced with moringa (MOR20), subabul (SBL20), guava (GVL20), moringa and subabul mixture (MS20) and moringa and guava mixture (MG20), by including them at 20%. Animals were fed with respective concentrate mixtures to meet about 80% of protein requirements and sorghum stover was offered ad libitum to meet the nutrient requirements of lambs for maintenance and growth as per ICAR (2013). Result: Dietary inclusion of tree leaves had no effect on the intake of various nutrients viz. DM, OM, CP, EE, CF, NFE, NDF,ADF, Hemicellulose and cellulose and were comparable (P greater than 0.05) among the six dietary groups. The crude protein and crude fibre digestibility improved (P less than 0.01) in tree leaves included diets compared to control, while ether extract (EE) digestibility was lower (P less than 0.01) in tree leaves included diets compared to CON. The DCP, TDN (%) and ME content was higher (P less than 0.05) in MS20, MG20 and MOR20 groups compared to CON. The N intake (g/d), excretion through faeces and urine (g/d), total N excretion and total N balance (g/d) was comparable among the different dietary groups.

A Review of Plant Growth Promoting Rhizobacteria and Their Characteristics as Potential Biofertilizer

The growing demand for agricultural products for food requirements caused the use of excessive inorganic chemical fertilisers, insecticides, fungicides, and pesticides for a quick and simple way to maximise and boost crop yield. This practice harmed food safety and caused the degradation of environmental, physical, and biological conditions. It has become alarming, and now is the time for a greener approach to increase agricultural output while minimising the use of inorganic chemical fertilisers. It was proven through many previous studies that using environmentally friendly biofertilisers has managed to increase crop yield while reducing the usage of chemical fertilisers. Plant growth-promoting rhizobacteria (PGPR) are mostly used in biofertiliser production because these types of microbes will enhance plant growth and yield by mobilising the available nutrients through several biological mechanisms, including fixation of atmospheric nitrogen, solubilisation, and mobilisation of phosphate and potassium, phytohormones production, disease suppression, and stress protection. Understanding their characteristics, biological mechanisms of action, and the nutritional and physical requirements for growth is important for successfully formulating and applying PGPR as a biofertiliser. The selection of the right PGPR with the desired characteristics, the ability to adapt to the environment, and the ideal formulation of the biofertiliser are the main criteria that should be emphasised when determining the success of biofertiliser. Knowledge and awareness regarding the use, benefits, and production of PGPR as a potential biofertiliser are important and should be explored to fulfil the crop’s nutritional requirements more economically and sustainably.

How has the cost of feeding New Zealand children well changed from 2018 to 2023? Monthly price changes of a modelled lower-cost healthy diet

ABSTRACT New Zealand (NZ) specific research assessing price changes of accessible lower-cost foods which also meet healthy eating guidelines for children is sparse. We aimed to develop an Excel™ tool to retrospectively assess weekly costs of commonly purchased lower-cost foods for children and households from 2018, and update in close to real-time. Lower-cost foods from the monthly reports from StatsNZ were grouped into food baskets that met eating guidelines, nutrient and energy requirements by age and sex. Repeated iterations created healthy food baskets from 62 lower-cost foods, prices were tracked, and costs for households and individuals were compared to household income. For a household of two adults and two children of fixed age (7y female, 14y male), substantial (27%) increases in the price of the food basket were recorded between 2021 and 2023. When the nutritional requirements of children as they age were considered, there was a 54% ($5,663) increase in the low-cost healthy food basket price of $16,083/year in 2023 compared to $10,420/year in 2018 when the female was 2y and male 9y. The continual increase in food prices contributes to substantial financial shortfalls for households receiving government support and the potential food insecurity many face.

Dietary fiber source and direct-fed microbial supplementation effects on lactation performance and feeding behavior of high-producing dairy cows

The objective of this study was to evaluate the effects of dietary fiber source and direct-fed microbial supplementation on lactation performance and feeding behavior of high-producing dairy cows. Sixty-four multiparous Holstein cows (3.5 ± 1.6 lactations; 76 ± 22 DIM and 735 ± 67 kg of BW at covariate period initiation) and 32 gate feeders were enrolled in a study with a completely randomized design and a 2 × 2 factorial arrangement. Cows and gate feeders were randomly assigned to treatments (16 cows and 8 gate feeds per treatment). Cows were allowed 1 week to acclimate to feeding gates followed by a 2-week covariate period. During the acclimation and covariate periods, all cows were fed the same diet to meet or exceed the nutrient requirements. Following the covariate period, cows were enrolled in a 8-week treatment period during which cows were randomly assigned to 1 of 4 treatments consisting of forage inclusion in the diet, either 45.8% (LF) or 56.7% (HF) of DM, and the supplementation of 75 mL/hd/d of a direct-fed microbial (DFM) containing herbal extracts (mallow, mint, and sage), L. plantarum, L. buchneri, Saccharomyces cerevisiae, and sugar cane molasses (Valibiom Mix, Valibiotics, Traiskirchen, Austria) or without supplementation (CON). The average covariate period value of each variable was used as a covariate. Three-way interactions were observed for DMI and feed efficiency. Dry matter intake was 2 to 3 kg greater for LF-DFM than HF-CON and HF-DFM during wk 2, 3, 5, and 8 of the treatment period. Milk production was 2.1 kg/d greater for LF than HF diets. Both milk fat and MUN concentrations were greater for HF than LF diets. Conversely, milk protein concentration was lower for HF than LF diets. The respiration rate measured in the morning was lower with DFM supplementation than CON. Rectal temperature measured in the morning and averaged for the day were greater for LF than HF diets. Under the conditions of the present study, feeding high-forage diets may be an alternative for producers to reduce feeding costs depending on the price of purchased feeds. However, non-forage fiber sources (i.e., soy hulls) must be considered when producers are challenged by either forage shortages or forage with a lower nutritive value. Additionally, DFM supplementation reduced respiration rate in the morning and affected meal behavior of lactating cows.

A Novel Facet of In-Hospital Food Consumption Associated with Hospital Mortality in Patients with Scheduled Admission-Addition of a Study Protocol to Test the Existence of Effects of COVID-19 in the Same Study in the Post-COVID-19 Period.

Humankind has faced unexperienced pandemic events since 2020. Since the COVID-19 pandemic has calmed down, we felt the need to verify whether in-hospital mortality had worsened compared to pre-pandemic conditions due to the COVID-19 pandemic. To test the hypothesis that daily food consumption is associated with in-hospital mortality during hospitalization and to provide baseline data to examine whether the effects of COVID-19 exist or not in post-pandemic period. All hospitalized patients staying in a single institution on the third Thursday of May, August, November, and the following February were included. Compared data: (1) among four seasons, (2) between age < 75 vs. ≥75 years, (3) between <75% vs. ≥75% of in-hospital food, and (4) logistic regression analysis to identify factors associated with in-hospital mortality. In 365 inpatients, the following results were obtained: (1) no seasonality or age effect in in-hospital mortality, (2) the novel cutoff value of 75% of the hospital food requirement was used to identify poor in-hospital survivors, (3) logistic regression analysis showed low food consumption, with <75% of the hospital food requirement as the predictor of high in-hospital mortality. A small eater of in-hospital food < 75% during hospitalization was associated with significantly higher in-hospital mortality in patients with scheduled hospitalization in the pre-pandemic period. Then, a study protocol is proposed to test the existence of the effects of COVID-19 in the same study in the post-COVID-19 period. This study protocol is, to our knowledge, the first proposal to test the effects of food consumption in the post-COVID-19 period on in-hospital mortality in the clinical nutritional areas.

Supply organ development of young broilers in response to increased carbohydrates and amino acids in the starter period

The growth of broiler chickens is marked by high fluctuations, varying nutrient requirement, early growth is characterized by high allometric growth rates of supply organs, which if underdeveloped, can impede nutrient efficiency and growth of demand organs like muscle and skeleton. This study aimed to investigate the impact of carbohydrate- and amino-acid-rich diets on the development of supply organs in broiler chickens. Four dietary treatments were used in a 2 × 2 factorial arrangement of treatments with apparent metabolizable energy (AME) at 2 levels (low: 2,750 kcal/kg and high: 3,050 kcal/kg) and standardized ileal digestible (SID) lysine at 2 levels (low: 1.0% and high: 1.2%) in the starter diets. Feed intake (FI) and BW gain were measured weekly; dissections were conducted at d 4 and d 11 to determine supply organ weights. Allometric growth of the liver was higher (P < 0.001) in the high AME and low lysine group compared to the other groups. For the pancreas, the highest (P < 0.001) allometric growth rate was in the high lysine groups. The small intestines responded differently; the duodenum had the highest (P < 0.001) allometric growth rate in the high AME groups and the jejunum in the low lysine groups, whereas the ileum showed an effect of diet density. For performance, high AME from carbohydrates, via maize starch, had a negative effect (P < 0.001) on FI and BW gain. High lysine had a positive effect (P < 0.001) on BW gain and FI, and high lysine alleviated part of the detrimental effect of high AME from carbohydrates. This effect was visible from d 0 to d 11, and persisted till the end of the trial on d 35. In conclusion, feeding a diet with a high AME from carbohydrates has negative consequences for the development of the supply organs of broilers.

Metabolic phenotyping of healthy and diseased human RPE cells.

Metabolic defects in the retinal pigment epithelium (RPE) underlie many retinal degenerative diseases. This study aims to identify the nutrient requirements of healthy and diseased human RPE cells. We profiled nutrient utilization of various human RPE cells, including differentiated and dedifferentiated fetal RPE (fRPE), induced pluripotent stem cell derived-RPE (iPSC RPE), Sorsby fundus dystrophy (SFD) patient-derived iPSC RPE, CRISPR-corrected isogenic SFD (cSFD) iPSC RPE, and ARPE-19 cell lines using Biolog Phenotype MicroArray Assays. Differentiated fRPE cells and healthy iPSC RPE cells can utilize 51 and 48 nutrients respectively, including sugars, intermediates from glycolysis and tricarboxylic acid (TCA) cycle, fatty acids, ketone bodies, amino acids, and dipeptides. However, when fRPE cells lose their epithelial phenotype through dedifferentiation, nutrient utilization becomes restricted to 17 nutrients, primarily sugar and glutamine-related amino acids. SFD RPE cells can utilize 37 nutrients; however, compared to cSFD RPE and healthy iPSC RPE, they are unable to utilize lactate, some TCA cycle intermediates, and short-chain fatty acids. Nonetheless, they show increased utilization of branch-chain amino acids (BCAAs) and BCAA-containing dipeptides. Dedifferentiated ARPE-19 cells grown in traditional culture media cannot utilize lactate and ketone bodies. In contrast, nicotinamide supplementation promotes differentiation towards an epithelial phenotype, restoring the ability to use these nutrients. Epithelial phenotype confers metabolic flexibility to healthy RPE for utilizing various nutrients. SFD RPE cells have reduced metabolic flexibility, relying on the oxidation of BCAAs. Our findings highlight the potentially important roles of nutrient availability and utilization in RPE differentiation and diseases.

Impact of light and nutrient availability on the phagotrophic activity of harmful bloom-forming dinoflagellates

Abstract Phagotrophy is a key nutritional mode for many bloom-forming dinoflagellates that can supplement their carbon and nutrient requirements. However, the environmental drivers and ecological relevance of phagotrophy in algal blooms are still poorly understood. This study evaluates the effect of light and nutrient availability on the phagotrophic activity of three common bloom-forming dinoflagellates (Alexandrium minutum, Heterocapsa triquetra and Prorocentrum micans) using three fluorescently labeled preys: bacteria, Synechococcus and the haptophyte Isochrysis galbana. The three dinoflagellates exhibited distinct responses to light and nutrient availability in terms of growth, cell size, prey ingestion and preference. A. minutum and H. triquetra showed higher cell-specific ingestion rates on bacteria (0.53 ± 0.13 and 1.64 ± 0.39 prey dinoflagellate−1 h−1, respectively) under co-limited nutrient and light availability, whereas P. micans showed higher ingestion on Synechococcus (0.93 ± 0.22 prey dinoflagellate−1 h−1) under low-light availability alone. However, the three dinoflagellates exhibited the highest carbon and nitrogen-specific ingestion rates when feeding on the larger prey I. galbana. Our findings indicate that phagotrophy could be of advantage in short periods of light or nutrient limitation and may play different roles during the development of blooms, likely influencing the energy transfer through the food web.

A Study on Genetically Engineered Foods: Need, Benefits, Risk, and Current Knowledge.

Food requirements have always been a top priority, and with the exponential growth of the human population, there is an increasing need for large quantities of food. Traditional cultivation methods are not able to meet the current demand for food products. One significant challenge is the shortened shelf-life of naturally occurring food items, which directly contributes to food scarcity. Contaminating substances such as weeds and pests play a crucial role in this issue. In response, researchers have introduced genetically engineered (GE) food as a potential solution. These food products are typically created by adding or replacing genes in the DNA of naturally occurring foods. GE foods offer various advantages, including increased quality and quantity of food production, adaptability to various climatic conditions, modification of vitamin and mineral levels, and prolonged shelf life. They address the major concerns of global food scarcity and food security. However, the techniques used in the production of GE foods may not be universally acceptable due to the genetic alteration of animal genes into plants or vice versa. Additionally, their unique nature necessitates further long-term studies. This study delves into the procedures and growth stages of DNA sequencing, covering the benefits, risks, industrial relevance, current knowledge, and future challenges of GE foods. GE foods have the potential to extend the shelf life of food items, alleviate food shortages, and fulfill the current nutritional food demand.

Site Specific Nutrient Management (SSNM): Principles, Key Features and its Potential Role in Soil, Crop Ecosystem and Climate Resilience Farming

As the world population expands, farming practices have intensified to meet increased food demand. Modern crop varieties now require higher levels of fertilization compared to traditional organic methods, which are insufficient to meet these new demands. However, inefficient nutrient management and imbalances in field nutrient levels have led to various crop issues. To achieve higher agricultural output and productivity, there has been a notable increase in the application of chemical fertilizers, which unfortunately contributes to soil and water pollution, degradation of soil fertility, and nutrient imbalances. In response, modern technologies are being leveraged to promote sustainable food production and foster balanced agricultural development. Site specific nutrient management (SSNM) aims to empower farmers to adjust fertilizer application dynamically to meet the nutrient requirements of high-yielding crops, bridging the gap between natural nutrient sources like soil, crop residues, manure, and irrigation water. It is based on the principles of 4Rs: right product, right dose, right time and right place. Modern technologies like Optical sensors, Crop Manager, Remote Sensing, Nutrient Expert®, fertility mapping, Active Canopy Sensors, Variable rate technologies etc. are used to enhance the potential of SSNM to higher level. The focus is on optimizing nutrient application timing and rates to maximize crop yield and nutrient efficiency, thereby enhancing the economic value of harvest per unit of fertilizer used, rather than simply increasing or decreasing fertilizer use.

Compositional nutrient analysis of greenhouse grown ryegrass (Lolium multiforum) fertilized with raw and hydrothermal-treated igneous rock as diagnostic tool for balanced fertilization in tropical environments

Due to the escalating demand for conventional potassium sources, and their unavailability in many African countries, there is a growing interest in hydrothermally treated igneous rocks as viable alternatives. These materials exhibit promising potential in enhancing potassium release, comparable to traditional sources like muriate of potash (M.O.P.). However, it remains uncertain whether they can adequately fulfill the nutrient requirements of plants. In this study, we evaluated nutrient balance in ryegrass (Lolium multiflorum) using raw and hydrothermally treated K-bearing silicate rocks as fertilizers. The Compositional Nutrient Diagnostic (CND) approach was used to assess the plants’ nutrient status, involving identification of high-yield subpopulations, and setting a yield cutoff. We derived a theoretical threshold for nutrient imbalance (CNDr2) using statistical methods like the chi-square distribution function and low-yield subpopulation proportion. CNDr2 was validated via Cate–Nelson partition and sum of squared individual nutrient indexes. Results showed a yield cutoff of 37.759 kg ha−1, distinguishing low-yield and high-yield subpopulations based on cumulative variance ratio functions from survey data. The theoretical threshold derived using the chi-square function was 10.1, subsequently validated by the Cate–Nelson method. Critical CND nutrient indices were symmetrical around zero, but their sum indicated foliar nutrient imbalance, notably zinc, potassium, and copper excess, attributed to 20 interactions identified (4 synergetic and 16 antagonistic). This study underscores hydrothermal treatment’s efficacy in improving nutrient availability and achieving a balanced nutrient profile compared to raw materials. It offers a promising solution for enhancing agricultural productivity, especially in tropical regions like Africa, where traditional sources are scarce.

Numerical Data Based Fertilizer Recommendation System for Farmer

In modern agriculture, optimizing fertilizer use is crucial for maximizing crop yields, maintaining soil health, and reducing environmental impact. The Numerical Data-Based Fertilizer Recommendation System (NDFRS) for farmers addresses these needs by leveraging data-driven approaches to provide precise fertilizer recommendations tailored to specific crop and soil conditions. This system integrates soil nutrient analysis, crop nutrient requirements, and environmental factors to generate customized fertilizer plans. The NDFRS employs advanced machine learning algorithms to analyze extensive datasets, including soil test results, historical crop yield data, weather patterns, and geographical information. By incorporating these variables, the system can predict the optimal type and amount of fertilizers required for different crops at various growth stages. This ensures that nutrients are supplied efficiently, enhancing crop productivity while minimizing excess fertilizer use and associated environmental risks. The user- friendly interface of the NDFRS allows farmers to input relevant data easily and receive actionable recommendations. Additionally, the system offers real-time updates and adaptive recommendations based on changing environmental conditions and crop growth stages. Field trials and validation studies demonstrate the system's efficacy in improving crop yields and promoting sustainable farming practices. Keywords: Tensor Flow, Deep Learning.

Update of Mineral Fertilization Recommendations for Maize (Zea mays L.) in the Savannah Region of Togo

The recommendations specific site of the nutrients in maize cultivation are necessary in the region of Savannah in Togo in the current context of the variability of the endogenous fertility of soils. To this end, based on the results of subtractive trials whose treatments were: the absolute control - N0P0K0 (T1), N0P60K70 (T2), N120P0K70 (T3), N120P60K0 (T4) and N120P60K70 (T5) kg ha-1 coupled with the maize variety Ikenne, conducted in the prefectures of the Savannah region, target yields were determined taking into account the potential yield of the Ikenne variety. Fertilization formulas to obtain the difference between the target yields and those measured on the zero N, zero P and zero K treatments were calculated. The results revealed that the gradient of priority nutrient requirement for maize was N &gt; P &gt; K in the prefectures. In Tandjouaré, grain yields of 3, 3.5, 4, and 4.5 Mg ha-1 were obtained with the fertilizer formulas N92P0K0, N112P10K0, N132P18K16 and N152P26K29 kg ha-1, respectively, with corresponding value/cost ratios of 16, 13, 11, and 10. In Tône, achieving grain yields of 2.5, 3, 3.5, 4, and 4.5 Mg ha-1 is subject to the fertilizer formulas N79P24K0, N99P32K16, N119P41K30, N139P49K43 and N159P57K56 kg ha-1, respectively, with corresponding value/cost ratios of 9, 8, 12, 7, and 6. In Oti, achieving grain yields of 3, 3.5, 4, and 4.5 Mg ha-1 is subject to the fertilizer formulas N66P7K0, N86P16K9, N106P24K22 and N126P32K36 kg ha-1, respectively, with corresponding value/cost ratios of 19, 14, 11, and 10. In Kpendjal, obtaining grain yields of 2.5, 3, 3.5, 4 and 4.5 Mg ha-1 is subject to fertilizer formulas N70P16K5, N90P24K18, N110P33K31, N130P41K45 et N150P49K58 kg ha-1, respectively, with corresponding value/cost ratios of 11, 9, 8, 7 and 7.

Mealworm Larvae Frass Exhibits a Plant Biostimulant Effect on Lettuce, Boosting Productivity beyond Just Nutrient Release or Improved Soil Properties

There is a need for alternatives or complements to synthetic fertilizers to enhance agricultural sustainability. Applying organic amendments can play a significant role in this. Insect droppings show high potential, though studies evaluating their agronomic value have only recently begun to emerge. This study compared black soldier fly (Hermetia illucens L.) and mealworm (Tenebrio molitor L.) larvae frass with another organic amendment (Nutrimais) derived from composting forestry, agro-industrial, and domestic waste. The experiment also included ammonium nitrate at two rates [the same as the organic amendments, 50 kg ha–1 nitrogen (N) (FullR), and half that rate (HalfR)] and an unfertilized control. The study spanned two growth cycles of lettuce (Lactuca sativa L.) grown in pots, followed by unfertilized oats (Avena sativa L.) to assess the residual effects of the fertilizing treatments. Mealworm larvae frass mineralized rapidly, with an apparent N recovery of 37.4% over the two lettuce growth cycles, indicating its high availability to soil heterotrophic microorganisms. The average dry matter yield (DMY) of lettuce was the highest among all treatments (12.8 and 9.8 g plant–1 in the first and second lettuce cycles), even compared to the FullR treatment (12.2 and 7.8 g plant–1), though without significant differences. Although mealworm larvae frass exhibited a high mineralization rate, the DMY cannot be attributed solely to N supply, as plants in the FullR treatment showed better N nutritional status. Mealworm larvae frass provided strong evidence of a plant biostimulant effect, not explained by the variables measured in this study. Black soldier fly larvae frass exhibited typical behavior of a moderately reactive organic amendment, while Nutrimais showed low reactivity, with a near-neutral mineralization/immobilization balance. The results suggest mealworm larvae frass is recommended for early maturing vegetable crops, whereas Nutrimais appears more suitable for perennial crops with low short-term nutrient requirements.

Potential Nutrient Contribution of Community-Based Insects in Children’s Food in Northern Ghana

BackgroundMicronutrient deficiencies are a major problem among children in northern Ghana. Available local foods and existing plant-based dietary patterns among children are insufficient to meet children’s nutrients requirements. Aside enhancing diets with animal source foods, most of which are expensive for rural households, entomophagy, which is culturally accepted, appears to be a great alternative. ObjectivesThis study aimed to 1) document the types of insects commonly consumed and the reasons for or against entomophagy in the study area, 2) document the reasons for adding or not adding insects to household meals, and 3) determine the nutrient contribution of community-based insects in children's food. MethodsBoth qualitative and quantitative research methods were concurrently applied in this exploratory study (N = 392 individuals; 6 focus group discussions) in northern Ghana. ResultsTermites, crickets, grasshoppers, and caterpillars were recognized as the most prevalent edible insects in communities. These insects were largely consumed by children but presently only included in household meals by a few households. Individual, sociocultural, sensory characteristics of insects, climate, and economic aspects were cited as grounds for and against entomophagy. Existing community-based children’s diets were unable to meet the acceptable recommended nutrient intake (RNI; within a given age and gender group, the RNI is the amount of a nutrient ingested daily that would meet the needs of almost all healthy individuals in that group) level of all nutrients under consideration, especially for zinc, vitamin B-12, folate, and fat. Inclusion of community-based edible insects increased the RNI levels for all 11 micronutrients considered and met children’s zinc, vitamin B-12, folate, and fat requirements. ConclusionsCommunity-based insects demonstrate a great potential for meeting micronutrients needs of children in the research setting. Future research is required to improve households’ adoption of community-based insects as part of household meals and to make insects accessible to households.

Optimizing winged-bean meal through oven and autoclave heating as viable alternative for plant-based aquafeed

Winged-bean meal can serve as a substitute for soybean meal in plant-based fish feed, despite its trypsin-inhibitor properties, which can be alleviated through heating processes. This study aimed to explore the potential of winged-bean meal subjected to different heating methods, namely oven (110 °C for 30 minutes) and autoclave (121 °C for 30 minutes), based on proximate analysis and trypsin activity. The study applied three treatments with four replications: oven heating (O), autoclave heating (A), and control (without heating, E). All data were analyzed using analysis of variance and Duncan’s multiple range test to determine the best treatment. Data were also compared to the nutrient requirements of several cultured freshwater fish in Indonesia. Heating treatments improved in vitro trypsin activity (1300 units/O and 1135 units/A vs. 835 units) but reduced protein (29.05±1.96%/A and 31.73±1.52%/O vs. 35.17±0.38%) and moisture contents. Additionally, the O treatment met the standards for aquafeed protein content (27-45%) and potentially fulfilled the protein requirements for catfish (25-40%), Nile tilapia (30-35%), and Java barb (30-32%). Therefore, oven heating presents a viable candidate as an alternative plant-based aquafeed ingredient. Nevertheless, the in vivo application of winged-bean meal as an aquafeed ingredient should be further evaluated to determine whether heating treatment effectively enhances digestive activity, nutrient retention, and growth performance in target fish species.

Techno-economical valorization of sugarcane bagasse for efficiently producing optically pure D-(−)-lactate approaching the theoretical maximum yield in low-cost salt medium by metabolically engineered Klebsiella oxytoca

Sugarcane bagasse (SCB) was utilized for efficiently producing optically pure D-(−)-lactate by Klebsiella oxytoca KIS004-91T strain. Cellulase (15 U/g NaOH-treated SCB) sufficiently liberated high sugars with saccharifications of 79.8 % cellulose and 52.5 % hemicellulose. For separated hydrolysis and fermentation, D-(−)-lactate was produced at 53.5 ± 2.1 g/L (0.98 ± 0.01 g/g sugar utilized or 0.71 ± 0.01 g/g total sugars) while D-(−)-lactate at 47.2 ± 1.8 g/L (0.78 ± 0.03 g/g sugar used or 0.69 ± 0.01 g/g total sugars) was obtained under simultaneous saccharification and fermentation (SSF). D-(−)-lactate at 99.9 ± 0.9 g/L (0.97 ± 0.01 g/g sugar utilized or 0.78 ± 0.01 g/g total sugars) was improved via fed-batch SSF. Based on mass balance, raw SCB of 7 kg is required to produce 1 kg D-(−)-lactate. Unlike others, D-(−)-lactate production was performed in low-cost salt medium without requirements of rich nutrients. Costs regarding medium, purification, and waste disposal may be reduced. This unlocks economic capability of SCB bioconversion or agricultural and agro-industrial wastes into high valuable D-(−)-lactate.

A novel ultra-performance liquid chromatography detection method development and validation for paclitaxel and its major metabolite in human plasma.

Paclitaxel is a promising anticancer drug for patients with ovarian, breast, lung, gastrointestinal, genitourinary, prostate, and head-and-neck cancers. Paclitaxel follows nonlinear pharmacokinetics. The major metabolite of paclitaxel is 6-alpha-hydroxy paclitaxel, mediated by CYP2C8, while metabolism to two of its minor metabolites, 3'-p-hydroxypaclitaxel and 6a, 3'- p-dihydroxypaclitaxel, is catalyzed by CYP3A4. Therapeutic drug monitoring of paclitaxel could be a promising approach to improve the efficacy and safety of paclitaxel correct personalized doses and improve the overall benefit-risk ratio. A novel and highly sensitive chromatographic method for the detection of paclitaxel and its metabolite has been proposed that allows quantification in human plasma with 100% accuracy in terms of recovery without significant intraday or interday variations. The present study was planned following bioanalytical method validation guidance according to the U.S. Food and Drug Administration requirements. The validation of the analytical procedure was performed as per ICH Q2(R1) guidelines. It was done to assure the reliability of the results obtained for various parameters such as linearity, accuracy, precision, limit of detection (LOD), limit of quantification, robustness, stability, and system suitability. The specificity of the method was established by ensuring no interference with peak obtained from paclitaxel and 6-alpha-hydroxy paclitaxel. LOD was found to be 0.05 and 0.033 while the limit of quantitation was 0.14 and 0.099 for paclitaxel and 6-alpha-hydroxy paclitaxel, respectively. Median (±interquartile range) accuracy for paclitaxel and 6-alpha-hydroxy paclitaxel was found to be 102.73 (±13.581) and 100.87 (±7.573), respectively. This novel method of simultaneous detection of paclitaxel and its major metabolite 6-alpha-hydroxy paclitaxel demonstrated significant resolution and was sensitive enough for its quantification in human plasma.

Mapping nutrient and soil fertility indexes for Durum Wheat in the La Mina region of Algeria

The growing population coupled with a reduction in available agricultural land per capita necessitates smart management practices to boost production per unit area and enhance overall land productivity, ultimately ensuring food security. Thus, evaluating soil fertility and productivity is crucial for sustainable agriculture. This study aims to assess the chemical fertility status of the soils within the irrigated perimeter of la Mina, a region in Algeria known for Durum Wheat production. Thirty-two soil samples were collected from the study area and subjected to analysis for total N, available P and K, and organic matter (OM) concentration. All the soils were basic (pH 7.63–8.95), had low concentrations of total N (0.04 and 0.19 %), low concentrations of available P (7.36 – 30 mg kg−1), low concentrations of available K (<0.4 Cmol kg−1) and low concentrations of OM (0.67–2.37 % by weight). Using a Nutrient Index classification, the overall classification of the soils of the irrigated perimeter of la Mina were low for N, P, K and OM. In addition, the Soil Fertility Index revealed three levels of fertility with a dominance of fertility level 5 (low). Application of the FertiliCalc software for the evaluation of nutrient requirements of Durum Wheat showed average requirements of 183 kg ha−1 for N, 65.8 kg ha−1 for P and 579 kg ha−1 for K. In light of these findings, appropriate agricultural practices, including the addition of fertilizers, are required to build and maintain the needs of Durum Wheat in the study area.