Simple SummaryThe gustatory sense of animals discriminates nutrients from noxious substances. However, enzymes in saliva can digest food components, potentially transforming the quality and taste of foods, and consequently altering feeding behavior. In the omnivorous German cockroach, Blattella germanica, feeding is highly stimulated by sugars, which are ubiquitous in its indoor environment. Glucose-averse cockroaches accept complex sugars, but they perceive glucose as a noxious tastant and reject it. We demonstrated that during feeding, saliva digests complex sugars, releasing glucose, and as a result, glucose-averse cockroaches interrupt their feeding on complex sugars. Moreover, we discovered that salivary alpha-glucosidases contributed to the breakdown of complex sugars to glucose. Therefore, the evolutionary adaptation of glucose-aversion, which protects cockroaches from insecticide-containing baits by changing the taste of glucose from sweet to bitter, also dramatically limits the suitability of complex sugars to glucose-averse cockroaches. We highlight the importance of pre-oral and oral saliva-mediated digestion in insect feeding behavior and the importance of considering the effects of saliva in design of sugar-containing insecticide baits.Saliva has diverse functions in feeding behavior of animals. However, the impact of salivary digestion of food on insect gustatory information processing is poorly documented. Glucose-aversion (GA) in the German cockroach, Blattella germanica, is a highly adaptive heritable behavioral resistance trait that protects the cockroach from ingesting glucose-containing-insecticide-baits. In this study, we confirmed that GA cockroaches rejected glucose, but they accepted oligosaccharides. However, whereas wild-type cockroaches that accepted glucose also satiated on oligosaccharides, GA cockroaches ceased ingesting the oligosaccharides within seconds, resulting in significantly lower consumption. We hypothesized that saliva might hydrolyze oligosaccharides, releasing glucose and terminating feeding. By mixing artificially collected cockroach saliva with various oligosaccharides, we demonstrated oligosaccharide-aversion in GA cockroaches. Acarbose, an alpha-glucosidase inhibitor, prevented the accumulation of glucose and rescued the phagostimulatory response and ingestion of oligosaccharides. Our results indicate that pre-oral and oral hydrolysis of oligosaccharides by salivary alpha-glucosidases released glucose, which was then processed by the gustatory system of GA cockroaches as a deterrent and caused the rejection of food. We suggest that the genetic mechanism of glucose-aversion support an extended aversion phenotype that includes glucose-containing oligosaccharides. Salivary digestion protects the cockroach from ingesting toxic chemicals and thus could support the rapid evolution of behavioral and physiological resistance in cockroach populations.