Foliar Spray Research Articles

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave.

Pseudomonas syringae is a gram-negative plant pathogen that infects plants such as tomato and poses a threat to global crop production. In this study, a novel lytic phage infecting P. syringae pv. tomato DC3000, named phage D6, was isolated and characterized from sediments in a karst cave. The latent period of phage D6 was found to be 60min, with a burst size of 16 plaque-forming units per cell. Phage D6 was stable at temperatures between 4 and 40°C but lost infectivity when heated to 70°C. Its infectivity was unaffected at pH 6-10 but became inactivated at pH ≤ 5 or ≥ 12. The genome of phage D6 is a linear double-stranded DNA of 307,402bp with a G + C content of 48.43%. There is a codon preference between phage D6 and its host, and the translation of phage D6 gene may not be entirely dependent on the tRNA library provided by the host. A total of 410 open reading frames (ORFs) and 14 tRNAs were predicted in its genome, with 92 ORFs encoding proteins with predicted functions. Phage D6 showed low genomic similarity to known phage genomes in the GenBank and Viral sequence databases. Genomic and phylogenetic analyses revealed that phage D6 is a novel phage. The tomato plants were first injected with phage D6, and subsequently with Pst DC3000, using the foliar spraying and root drenching inoculum approach. Results obtained after 14days indicated that phage D6 inoculation decreased P. syringae-induced symptoms in tomato leaves and inhibited the pathogen's growth in the leaves. The amount of Pst DC3000 was reduced by 150- and 263-fold, respectively. In conclusion, the lytic phage D6 identified in this study belongs to a novel phage within the Caudoviricetes class and has potential for use in biological control of plant diseases.

Effect of Potassium Humate, Phosphorus and Copper on Onion Quantitative and Qualitative Yield

Previous research has been limited regarding the combined impacts of copper and potassium humate under various phosphorus fertilization levels on onion crops. So, a field trials were performed during two successive winter seasons of 2021 - 2022 and 2022 - 2023 aiming to evaluate three levels of calcium superphosphate (representing 100 %, 75 % and 50 % of the phosphorus recommended dose) as main plot treatments, along with three levels of potassium humate soil addition ( 0.0 %, 1.0 % and 2.0 % ) as sub-plot treatments. Additionally, foliar spraying of copper was carried out at three different rates (0.0 %, 0.1 % and 0.2 %) as sub-subplot treatments. These factors were examined individually or in combination to assess their impact on onion performance, quantitative yield, and qualitative attributes. Firstly, in terms of the individual effect of phosphorus recommended dose (PRD), the application of 100% of PRD demonstrated superior impacts on onion growth performance, quantitative and qualitative yield compared to 75% and 50% of PRD, respectively. Secondly, focusing on the individual effect of soil addition of potassium humate, all parameters related to growth performance, quantitative and qualitative yield, such as bulb weight and diameter, neck diameter, marketable bulb yield, total dissolved solids (TDS), total sugar (%), carbohydrates, and crude protein, exhibited an increase with higher rates of added potassium humate. Thirdly, the values of all parameters studied increased as the rate of copper application increased, indicating a positive correlation between copper application and onion crop performance. Lastly, the combined treatment of 100% of PRD with 2.0% potassium humate and 0.2% copper recorded the highest values across all measured parameters. Additionally, the combined treatment of 75% of PRD with potassium humate at rates of 1.0% or 2.0%, along with copper at rates of 0.1% and 0.2%, outperformed the individual application of 100% PRD in terms of the measured parameters. Therefore, it can be concluded that the combined addition of potassium humate (as soil addition) and Cu (as foliar application) has a vital role in improving the quantitative and qualitative yield of the as well as raising the efficiency of phosphate fertilizers.

Influence of Foliar Treatment with Suspensions Rich in Trichoderma Chlamydospores on Momordica charantia Physiology, Yield, and Quality

Several strategies promote phyllosphere colonization by soil-born Trichoderma plant-beneficial strains. One of these strategies is foliar spraying with suspensions containing large amounts of chlamydospores—spores with thick cell wall structures that make them highly resistant to harsh environmental conditions. Trichoderma biomass was produced by cultivation on a cornmeal medium and compared with the biomass produced on potato dextrose broth by microscopic and thermogravimetric analyses. The analyses revealed increased chlamydospore content and thermostability in the fungal biomass produced on the corn meal medium. The Trichoderma suspension rich in chlamydospores was sprayed on bitter gourd (Momordica charantia) leaves at two inoculant concentrations, 106 and 108 ufc/mL. The effect of these treatments on the plant physiological parameters, leaf photosynthetic pigments, polyphenol and flavonoid contents, antioxidant activities of the leaves and fruits, and yield was compared to the control (plants sprayed with water) and to the experimental treatment involving spraying with 108 ufc/mL of propagules produced in potato dextrose broth. The effect of chlamydospore-rich suspensions on plant physiological parameters was more pronounced and long-lasting compared with the other treatments. The treatment with chlamydospore-rich suspension enhanced the accumulation of polyphenols and flavonoids in the leaves (by 17% and 50%, respectively) and fruits (by 18% and 31%, respectively) and increased the antioxidant activity. The Trichoderma treatment increased the yield by +25.33–53.07%. The application of the foliar treatment with Trichoderma suspensions did not modify the cytocompatibility of the extracts from the fruits determined on the L929 cells.

Unraveling the effect of phenolic extract derived from olive mill solid wastes on agro-physiological and biochemical traits of pomegranate and its associated rhizospheric soil properties

Agricultural waste management poses a significant challenge in circular economy strategies. Olive mill wastes (OMW) contain valuable biomolecules, especially phenolic compounds, with significant agricultural potential. Our study evaluate the effects of phenolic extract (PE) derived from olive mill solid wastes (OMSW) on pomegranate agro-physiological and biochemical responses, as well as soil-related attributes. Pomegranate plants were treated with PE at doses of 100 ppm and 200 ppm via foliar spray (L100 and L200) and soil application (S100 and S200). Results showed increased biomass with PE treatments, especially with soil application (S100 and S200). Proline and soluble sugar accumulation in leaves suggested plant adaptation to PE with low-level stress. Additionally, PE application reduced malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents. Higher doses of PE (S200) significantly improved net photosynthesis (Pn), transpiration rate (E), water use efficiency (WUEi), and photosynthetic efficiency (fv/fm and PIabs). Furthermore, PE treatments enhanced levels of chlorophylls, carotenoids, polyphenols, flavonoids, and antioxidant activity. Soil application of PE also increased soil enzyme activities and microbial population. Our findings suggest the beneficial impact of PE application on pomegranate agro-physiological responses, laying the groundwork for further research across various plant species and soil types to introduce nutrient-enriched PE as an eco-friendly biostimulant.

Efficacy of Diamide Insecticides Delivered through Different Methods against Shoot and Fruit Borer L. orbonalis.

Many vegetables insect pests are managed by using conventional insecticides. Unfortunately, these insecticides are harmful to natural enemies, bees, predators, parasitoids and no longer have some of the insect pests have developed resistance to these insecticides. Diamides insecticides are the new generation insecticides that provide systemic way controlling many arthropod pest, but exhibit low toxicity to beneficial insects some other arthropod pests and may be promising alternative to other conventional insecticides. Field experiment was carried out to evaluate the efficacy of diamides delivered through different during Kharif, 2021-22 with 14 treatments and 2 replications. Diamides may be delivered to brinjal crop via seedling dipping, soil drenching and foliar spraying therefore it is important to determine the most effective pest control strategy while using the least amount of active ingredients. In a case study, chlorantraniliprole flubendiamide and cyantraniliprole were used to control brinjal shoot and fruit borer, Leucinodes orbonalis (Guenee) (Lepidoptera: Pyraustidae) in brinjal. These insecticides administered as foliar spray, soil drenching and seedling dipping were compared, the treatment with foliar application of diamides gave good results as compared to soil drenching and seedling dipping. The results indicated that, diamides were superior to other insecticides used in the present investigation but they failed to give good control when applied through seedling dipping method. Highest C:B ratio (1: 6.07) and yield (47.80t/ha) was recorded in foliar spray of chlorantraniliprole 18.5 SC treated plot.

Role of Nutrient Management in Yield, Quality and Nutrient Content of Egyptian Clover (Trifolium alexandrinum L.) Under Calcareous Soil Conditions

Nutrient management is vital for maximizing forage crop yield and economic viability; however, improper application can result in decreased yield and quality. Therefore, two seasonal experiments were conducted during the winter seasons of 2022-2023 and 2023-2024 to examine the impact of varying nitrogen, phosphorus and potassium (NPK) levels, along with the foliar application of zinc, on the forage yield and nutrient content of Egyptian clover or berseem (Trifolium alexandrinum L.) grown in calcareous soil conditions (>36% CaCO3). The experiment consisted of eight treatments, including two NPK levels (50% and 100% of the recommended dose) and two forms of Zn (Zn NPs and Zn Edta at 0.2 and 0.5% as foliar sprays), compared with the control (without treatment). Results showed that NPK full dose (100%) or in combination with Zn NPs significantly improved fresh and dry weight by an average of 67%, and 80%, respectively, across all cuts. Whereas, the highest protein percentage in berseem forage occurred with 50% NPK and foliar Zn NPs. Protein yield per unit area was highest with 100% NPK and foliar Zn NPs in the second and third cuts. Furthermore, Zn NPs boosted manganese (Mn) levels in the 2nd cut but decreased them in the 3rd cut. Iron (Fe) and Mn were mostly in normal ranges, but zinc was often below normal levels. Overall, Zn NPs increased forage yields. The results of translocation factor (TF) for micronutrients from the soil to berseem plants showed that TF values were > 1 for all micronutrients except for Cu in several instances in the 2nd cut in such calcareous soil. Furthermore, micronutrient translocations were arranged in the following order Cu < Zn <Mn <Fe in the 2nd and 3rd cuts. Our study suggested that there is a beneficial role of correcting Zn deficiency under calcareous soil conditions which is reflected on berseem yield and quality and nutrient status. Thus, using ZnO NPs along with the full recommended dose of NPK is a suitable approach to enhance berseem crop yield and quality in calcareous soil.

Effect of foliar spray of urea and Potassium Citrate on fruiting of Barhee Date palm

Effect of foliar spray of urea and Potassium Citrate on fruiting of Barhee Date palm

Exogenous ascorbic acid as a potent regulator of antioxidants, osmo-protectants, and lipid peroxidation in pea under salt stress

Pea (Pisum sativum L.), a globally cultivated leguminous crop valued for its nutritional and economic significance, faces a critical challenge of soil salinity, which significantly hampers crop growth and production worldwide. A pot experiment was carried out in the Botanical Garden, The Islamia University of Bahawalpur to alleviate the negative impacts of sodium chloride (NaCl) on pea through foliar application of ascorbic acid (AsA). Two pea varieties Meteor (V1) and Sarsabz (V2) were tested against salinity, i.e. 0 mM NaCl (Control) and 100 mM NaCl. Three levels of ascorbic acid 0 (Control), 5 and 10 mM were applied through foliar spray. The experimental design was completely randomized (CRD) with three replicates. Salt stress resulted in the suppression of growth, photosynthetic activity, and yield attributes in pea plants. However, the application of AsA treatments effectively alleviated these inhibitory effects. Under stress conditions, the application of AsA treatment led to a substantial increase in chlorophyll a (41.1%), chl. b (56.1%), total chl. contents (44.6%) and carotenoids (58.4%). Under salt stress, there was an increase in Na+ accumulation, lipid peroxidation, and the generation of reactive oxygen species (ROS). However, the application of AsA increased the contents of proline (26.9%), endogenous AsA (23.1%), total soluble sugars (17.1%), total phenolics (29.7%), and enzymatic antioxidants i.e. SOD (22.3%), POD (34.1%) and CAT (39%) in both varieties under stress. Salinity reduced the yield attributes while foliarly applied AsA increased the pod length (38.7%), number of pods per plant (40%) and 100 seed weight (45.2%). To sum up, the application of AsA alleviated salt-induced damage in pea plants by enhancing photosynthetic pigments, both enzymatic and non-enzymatic activities, maintaining ion homeostasis, and reducing excessive ROS accumulation through the limitation of lipid peroxidation. Overall, V2 (Sarsabz) performed better as compared to the V1 (Meteor).

Effect of Organic Manures and Foliar Application on Growth, Yield and Nutrient Uptake of Kalmegh (Andrographis paniculata Nees.)

A field experiment was carried out to study the effect of organic manures and foliar application of organics on growth, yield and nutrient uptake of Kalmegh at the Department of Horticulture, Faculty of Agriculture, Annamalai University to find out the suitable organic inputs and foliar organics for better growth, yield and nutrient uptake of Kalmegh. The experiment was laid out in a Randomized Block Design with twelve treatments and three replications comprising of a different combination (i) basal organics viz., Farm Yard Manure, Vermicompost and Pressmud (ii) foliar spray of organics viz., Panchagavya and Fish Amino Acid and untreated control. Among the twelve treatments, the plants which received Vermicompost at 5 t ha-1 + fish amino acid at 3% expressed higher values for the growth parameters viz., plant height (69.80 cm), number of branches plant-1 (25.13), number of leaves plant-1 (140.87). Physiological parameters viz., leaf area plant-1 (6.78), total dry matter production plant-1 (15.86 g plant-1). Yield parameters viz., fresh herbage yield plant-1 (42.74 g plant-1) and dry herbage yield plant-1 (14.24 g plant-1). Nutrient uptake viz., Nitrogen (38.97 Kg ha-1), Phosphorous (6.87 Kg ha-1) and Potassium (84.23 kg ha-1).

Redox priming could be an appropriate technique to minimize drought-induced adversities in quinoa.

The exogenous use of the redox compound (H2O2) plays a significant role in abiotic stress tolerance. The present study investigated various H2O2 application methods (seed priming, foliar spray, and surface irrigation) with varying concentration levels (0 mM, 5 mM, 10 mM, 15 mM, 40 mM, 80 mM, and 160 mM) to evaluate the efficiency of supplying exogenous H2O2 to quinoa under water-deficit conditions. Drought stress reduced quinoa growth and yield by perturbing morphological traits, leading to the overproduction of reactive oxygen species and increased electrolyte leakage. Although all studied modes of H2O2 application improved quinoa performance, surface irrigation was found to be sensitive, causing oxidative damage in the present study. Seed priming showed a prominent increase in plant height due to profound emergence indexes compared to other modes under drought conditions. Strikingly, seed priming followed by foliar spray improved drought tolerance in quinoa and showed higher grain yield compared to surface irrigations. This increase in the yield performance of quinoa was attributed to improvements in total chlorophyll (37%), leaf relative water content (RWC; 20%), superoxide dismutase (SOD; 35%), peroxidase (97%), polyphenol oxidase (60%), and phenylalanine ammonia-lyase (58%) activities, and the accumulation of glycine betaine (96%), total soluble protein (TSP; 17%), proline contents (35%), and the highest reduction in leaf malondialdehyde contents (MDA; 36%) under drought stress. PCA analysis indicated that physio-biochemical traits (proline, SOD, TSP, total chlorophyll, MSI, and RWC) were strongly positively correlated with grain yield, and their contribution was much higher in redox priming than other application methods. In conclusion, exogenous H2O2 application, preferably redox priming, could be chosen to decrease drought-induced performance and yield losses in quinoa.

Loblolly Pine Mid-Rotation Phosphorus Fertilization with Poultry Litter Ash or Triple Superphosphate

ABSTRACT Loblolly pine (Pinus taeda L.) grown on infertile coastal plain soils typically shows a growth response to N and P applied at mid-rotation. The objectives of this study were to determine whether less soluble poultry litter ash (PLA) provides an adequate supply of P compared to triple superphosphate (TSP), and assess effects on P mineralization and runoff loss. Effects of 56 kg P ha−1 as either PLA or TSP, and no fertilization were compared using an 11-year-old stand of two loblolly pine genotypes on a loamy sand in a completely randomized design. Tree height, diameter and foliar nutrients, soil Mehlich 3 P and phosphatase activity (A and E horizons), and P in surface runoff were monitored. Through more than two years, P fertilization had no effect on tree growth. Similar foliar P with PLA or TSP, together with increasing foliar and soil P over time, suggested that PLA is an adequate source of P for loblolly pine. Neither fertilizer suppressed P mineralization as measured by phosphatase activity. The advantage of PLA compared to TSP is potentially much less runoff P loss.

Fulvic acid foliar application: a novel approach enhancing antioxidant capacity and nutritional quality of pistachio (Pistacia vera L.)

BackgroundThe global growth of pistachio production has prompted exploration into sustainable agricultural practices, on the application of humic substances such as fulvic acid in enhancing the quality of horticultural crops. The present study was carried out in Qom province, Iran, on 20 years old pistachio (Pistacia vera L. cv. Kaleh-Ghoochi) trees and investigated the impact of foliar spraying of fulvic acid at varying concentrations (1.5, 3, and 4.5 g L− 1) on the antioxidant and quality properties of pistachio. The different concentrations of fulvic acid were applied at two key stages: at the initiation of pistachio kernel formation (late June) and the development stage of pistachio kernel (late August), as well as at both time points. Following harvest at the horticulturally mature phase, various parameters, including total phenols, flavonoids, soluble proteins, soluble carbohydrate content, antioxidant capacity, and antioxidant enzyme activity, were assessed.ResultsResults indicated that foliar application of fulvic acid, particularly at 1.5 g L− 1 during both late June and August, effectively increased phenolic compounds (31.8%) and flavonoid content (24.53%). Additionally, this treatment also augmented antioxidant capacity and heightened the activity of catalase (CAT) (37.56%), ascorbate peroxidase (APX) (63.86%), and superoxide dismutase (SOD) (76.45%). Conversely, peroxidase (POX) (41.54%) activity was reduced in fulvic acid-treated nuts compared with controls. Moreover, the content of chlorophyll (45%) and carotenoids (46.7%) was enhanced using this organic fertilizer. In terms of mineral elements, the increment was observed in zinc (Zn) (58.23%) and potassium (K) (28.12%) amounts in treated nuts. Additionally, foliar application of fulvic acid led to elevated levels of soluble carbohydrates and proteins in treated nuts.ConclusionsIn the present study, application of fulvic acid resulted in enhancement of antioxidant activity and quality traits of pistachio nut through an increase in total phenol, flavonoids, chlorophyll, carotenoids, K, Zn, and also activity of antioxidant enzymes. Therefore, use of fulvic acid emerges as a promising strategy to enhance the quality and nutritional attributes of pistachios, contributing to sustainable agricultural practices and improved crop outcomes.

Utility of in situ ion‐exchange membranes to assess nutrient availability, productivity, and fertilizer response of coastal Douglas‐fir of the Pacific Northwest

AbstractDouglas‐fir [Pseudotsuga menziesii (Mirb.) Franco] is the predominant forest plantation species in the Pacific Northwest (PNW), with site productivity and fertilizer response influenced by climate and soil variations. This study investigates the utility of in situ 12‐week supply measurements of nitrogen (N), calcium (Ca), and phosphorus (P) to ion‐exchange resins (specifically Plant Root Simulator [PRS] probes) to estimate carbon (C):N ratios, soil nutrient contents (0–1 m), foliar nutrient concentrations, Douglas‐fir productivity (site index and basal area mean annual increment), and fertilizer volume response. PRS nutrient supply rates were correlated with N, Ca, and P soil nutrient contents (0–1 m), C:N ratios, and foliar nutrient concentrations. Low PRS NO3 supply rates (<25 mg N·m−2·burial period−1) were correlated with lower Douglas‐fir productivity and greater fertilizer volume response. PRS NO3 supply rates performed as well as total soil N contents and foliar N concentrations at estimating volume growth response to fertilizer. Twelve weeks after fertilization, PRS NO3, NH4, and Ca supply rates were significantly elevated compared to the unfertilized treatment. This research found that PRS probes were an effective in situ tool and are recommended for understanding N, Ca, and P nutrient availabilities, site productivity, and fertilizer response in Douglas‐fir plantations and for developing fertilizer prescriptions.

A Review on Effect of Organic Conditioner on Physico-chemical and Microbiological Properties of Soil

The unbalanced use of chemical fertilizers has caused adverse effects on the environment and human health, which has raised the focus on environmentally friendly agricultural techniques for sustainable farming globally. The article investigates the effects of organic conditioners on the physical, chemical, and microbiological characteristics of soil, with focus on liquid organic conditioners such Panchagavya, Jeevamruth, and Beejamruth. In order to purify agricultural fields, the necessity of organic agriculture methods that are both commercially and environmentally feasible is emphasized. The advantages of applying Soil conditioners are explained, including increased crop production, quality, affordability, and safety for the environment in addition to increases in soil health, fertility, and pest resistance. An overview of the basic principles of liquid organic manures is provided, with a focus on their ability to release nutrients, stimulate microbial activity, and facilitate plant absorption. The review discusses how organic conditioners might improve the biological characteristics of soil, such as the presence of chemicals that promote plant growth and helpful microbes. Liquid organic manure adoption is essential for healthy ecosystems, sustainable agriculture, and increased soil fertility and productivity. Because liquid organic manures may be applied in a variety of ways and used as soil drenches, foliar sprays, and seedling treatments, they are a crucial part of environmentally friendly farming methods. It is advised to combine liquid and solid organic manures to reduce negative effects and guarantee a robust and fruitful farming environment.

Enhancement of Growth and Yield of Cotton by Organic and Inorganic Nutrients with Phytohormone

Cotton is an important fibre and cash crop in India, contributing significantly to the country's industrial and agricultural economies. It provides the textile industry with its main raw material (cotton fibre). Cotton in India is a direct source of income for 6 million farmers, while the cotton trade and processing employs approximately 40-50 million people. Agriculture in the twenty-first century has encountered numerous hurdles in meeting the expanding population's food and fibre needs. Humans will confront greater challenges in meeting their requirements as the population grows to reach nine billion by 2050. A field experiment was conducted in Farmer’s Field at Jayapuram Village, Natrampalli Taluk in Thirupathur District during Aug-Jan (2021-22) to investigate the effect of soil application of NPK fertilizers, Zn-enriched compost and foliar spray of ZnO2 and NAA on enhancement of cotton yield and quality in sandy loam soil. Fertilizers (N: P2O5: K2O) were sprayed at the appropriate dose (60:30:30 kg ha-1) using Urea, SSP, and MOP. Zn-enriched compost (Zn-EC) was treated at 1.0 t ha-1 as a basal one week before sowing. As per treatment. ZnO2 at 0.1% was applied twice during the vegetative and blooming periods of the crop. The treatment included spraying NAA at 40 ppm twice during flowering and boll formation. Cotton cv. Sabari was cultivated as a test crop using suggested cultural methods. The highest seed cotton yield of 1813.73 kg ha-1 was observed with application of 75% RDF + Zn EC @ 1.0 t ha-1 + ZnO2 @ 0.1%+ NAA @ 40 ppm (T9). the present investigation clearly concluded that 75% RDF + Zn EC @ 1.0 t ha-1 + ZnO2 @ 0.1%+ NAA @ 40 ppm (T9) was the best treatment for enhancing growth, yield, quality and nutrient uptake by cotton cv. Sabari. The study examined how different treatments affected growth, yield characteristics, quality, and soil health.

A red seaweed Kappaphycus alvarezii-based biostimulant (AgroGain®) improves the growth of Zea mays and impacts agricultural sustainability by beneficially priming rhizosphere soil microbial community.

The overuse of chemical-based agricultural inputs has led to the degradation of soil with associated adverse effects on soil attributes and microbial population. This scenario leads to poor soil health and is reportedly on the rise globally. Additionally, chemical fertilizers pose serious risks to the ecosystem and human health. In this study, foliar sprays of biostimulant (AgroGain/LBS6) prepared from the cultivated, tropical red seaweed Kappaphycus alvarezii increased the phenotypic growth of Zea mays in terms of greater leaf area, total plant height, and shoot fresh and dry weights. In addition, LBS6 improved the accumulation of chlorophyll a and b, total carotenoids, total soluble sugars, amino acids, flavonoids, and phenolics in the treated plants. LBS6 applications also improved the total bacterial and fungal count in rhizospheric soil. The V3-V4 region of 16S rRNA gene from the soil metagenome was analyzed to study the abundance of bacterial communities which were increased in the rhizosphere of LBS6-treated plants. Treatments were found to enrich beneficial soil bacteria, i.e., Proteobacteria, especially the classes Alphaproteobacteria, Cyanobacteria, Firmicutes, Actinobacteriota, Verrucomicrobiota, Chloroflexi, and Acidobacteriota and several other phyla related to plant growth promotion. A metagenomic study of those soil samples from LBS6-sprayed plants was correlated with functional potential of soil microbiota. Enrichment of metabolisms such as nitrogen, sulfur, phosphorous, plant defense, amino acid, co-factors, and vitamins was observed in soils grown with LBS6-sprayed plants. These results were further confirmed by a significant increase in the activity of soil enzymes such as urease, acid phosphatase, FDAse, dehydrogenase, catalase, and biological index of fertility in the rhizosphere of LBS6-treated corn plant. These findings conclude that the foliar application of LBS6 on Z. mays improves and recruits beneficial microbes and alters soil ecology in a sustainable manner.

Enhancing Botrytis disease management in tomato plants: insights from a Pseudomonas putida strain with biocontrol activity.

This study explores the biocontrol potential of Pseudomonas putida Z13 against Botrytis cinerea in tomato plants, addressing challenges posed by the pathogen's fungicide resistance. The aims of the study were to investigate the in vitro and in silico biocontrol traits of Z13, identify its plant-colonizing efficacy, evaluate the efficacy of different application strategies against B. cinerea in planta, and assess the capacity of Z13 to trigger induced systemic resistance (ISR) in plants. The in vitro experiments revealed that Z13 inhibits the growth of B. cinerea, produces siderophores, and exhibits swimming and swarming activity. Additionally, the Z13 genome harbors genes that encode compounds triggering ISR, such as pyoverdine and pyrroloquinoline quinone. The in planta experiments demonstrated Z13's efficacy in effectively colonizing the rhizosphere and leaves of tomato plants. Therefore, three application strategies of Z13 were evaluated against B. cinerea: root drenching, foliar spray, and the combination of root drenching and foliar spray. It was demonstrated that the most effective treatment of Z13 against B. cinerea was the combination of root drenching and foliar spray. Transcriptomic analysis showed that Z13 upregulates the expression of the plant defense-related genes PR1 and PIN2 upon B. cinerea inoculation. The results of the study demonstrated that Z13 possesses significant biocontrol traits, such as the production of siderophores, resulting in significant plant protection against B. cinerea when applied as a single treatment to the rhizosphere or in combination with leaf spraying. Additionally, it was shown that Z13 root colonization primes plant defenses against the pathogen.

Silica nanoparticles improve growth, chemical bioactive, and antioxidant enzyme activity of Dianthus caryophyllus L., plant

Background Nanotechnology has provided innovative use of various materials with unique properties. These substances may react positively in plants, and the induction of bioactive compounds and antioxidant activity is particularly remarkable. Ornamental plant production is a positive economic activity of great interest, especially Carnation (Dianthus caryophyllus L.). This plant is more attractive for its various colors, but the flower quality and its longevity can diminish very fast. Thus, examining methods capable of improving flower quality and vase life is crucial. Additionally, carnation can be used to treat coronary and nervous disorders. Carnation contains compounds that calm the nervous system and minimize swelling and inflammation. Objective This research aimed to evaluate the effect of silica nanoparticles (SiO2 NPs) on the growth, bioactive chemical analysis, enzyme activity, and antioxidant accumulation. Material and methods The different rates of SiO2 NPs (0, 150, and 300 ppm) were used in two ways: foliar spray and soil drench; it was applied 3 times. The first application was after 3 weeks of transplanting and repeated with 21-day intervals. Results and conclusion The results showed that foliar application of SiO2 NPs increased all morphological and flowering traits compared with drench and the control, higher levels of photosynthetic pigments and anthocyanin, greater total phenols, total sugars, and total free amino acids were obtained from plants treated with foliar spray 300 ppm followed than 150 ppm. In addition, the greatest levels of enzyme activity were recorded with plants treated with foliar spray of SiO2 NPs at a rate of 300 ppm, as well as improved anatomical structure of the stem and stomata properties compared with the drench method and control. Similarly, SiO2 NPs application method is more critical than their concentration. Therefore, it is recommended that foliar spraying of SiO2 NPs at 300 ppm can enhance plant growth, flowering, bioactive compounds, enzyme activity, and anatomical structure of the stem of carnation plants while adding SiO2 NPs drench at a rate of 150 ppm had rather acceptable findings.

Influenced Quality of Wheat (Triticum aestivum L.) and Abate the Environmental Pollution by Soil Applied with Foliar Fertilization of NPK Fertilizer in Saline Soil

A field experiment to assess different levels and methods of NPK fertilizer application on wheat crop was conducted on loamy sand soil at Instructional farm, College of Agriculture, Bikaner during rabi season of 2019-2020. The trial was laid out in split plot design and replicated three times. A total of 20 treatment combinations comprises A. Main plot : four RDF levels prior to sowing viz., F0 = Absolute control, F1= 50% RDF, F2= 75 % RDF and F2= 100 % RDF and B. Sub plot : five foliar fertilization levels of soluble NPK (1%) namely S0= no fertilization (control), S1= two spray (At 45and 60 DAS), S2= three foliar spray (At 45, 60 and 75 DAS), S3= four foliar spray (At 45, 60, 75 and 90 DAS). Basal application of 100% RDF with three foliar spray of soluble NPK (19:19:19) at 45, 60 and 75 DAS gave significantly higher average grain and straw yield and N, P, K content and uptake in grain and straw than other treatments, including control.

Influence of Fertilization Methods and Types on Wheat Rhizosphere Microbiome Community and Functions.

To investigate the effects of fertilization methods and types on wheat rhizosphere microorganisms, macroelement (N, K) and microelement (Zn) fertilizers were applied on wheat by foliar spraying (FS) and root irrigation (RI) methods in a field experiment. The results indicated that fertilization methods and types can have significant impacts on the diversity and structure of rhizospheric microorganisms in wheat. The application method produced more significant effects than the fertilizer type. RI-N played a more important role in improving the wheat yield and quality and affected the changes in some nitrogen-fixing bacterial communities. Finally, eight strains of bacteria belonging to Pseudomonas azotoformans and P. cedrina showed positive effects on the growth of wheat seedlings. Overall, our study provides a better understanding of the dynamics of wheat rhizosphere microbial communities and their relation to fertilization, yield, and quality, showing that plant growth-promoting rhizobacteria with nitrogen fixing may be a potential approach for more sustainable agriculture production.