Fluoride Mouthwash Research Articles

Effective Fluoride Mouthwash Delivery Methods as an Alternative to Rinsing.

Special-needs patients with high caries risk cannot benefit from the proven caries-reducing effect of fluoride mouthrinse because of poor rinsing compliance and a lack of home-use fluoride application recommendations. This study aimed to evaluate whether two modified delivery methods-spray or swab application-could raise the salivary fluoride to levels similar to that of rinsing. Five healthy men and 16 healthy women, ages 18-22 years, participated in this crossover study. The subjects performed a standardized brushing routine twice a day with 1,000-ppm fluoride dentifrice 1 week before and during the experimental period. The three fluoride mouthwash (0.05% NaF; 226-ppm fluoride) administration methods consisted of rinsing, spray, and cotton swab application. Each application was separated by at least 1 week. Unstimulated whole saliva was sampled for 2 minutes at baseline and at 0, 5, 10, 20, 30, 60, and 120 minutes after each fluoride mouthwash application. Salivary fluoride level (ppm) was measured by a blinded investigator using an ion-specific electrode (ORION EA940). The differences in salivary fluoride concentration for each application method were analyzed by repeated measures ANOVA at a nominal significance level of .05. The newly designed fluoride mouthwash delivery methods yielded a similar fluoride level in whole saliva compared to that of rinsing at all time points (p > .05). The t half-life of fluoride concentration ranged from 1.62 minutes (SD = 0.53) in the spray group to 2.08 minutes (SD = 0.87) in the cotton swab group; the values were not significantly different. Our results indicate that the spray and cotton swab fluoride delivery methods were effective alternatives to conventional mouth rinsing. Caregivers of individuals with acute and chronic illness, including developmental disabilities affecting cognitive or physical abilities, may consider using these alternative fluoride delivery methods to help promote dental hygiene.

Effect of Chlorhexidine with Fluoride Mouthrinse on Plaque Accumulation, Plaque pH - A Double Blind Parallel Randomized Clinical Trial.

Mouthwashes are important means used in chemical control of dental plaque. There is strong evidence suggestive of better effectiveness, when fluoride is added to chlorhexidine mouthwash. To assess the anti-plaque efficacy of Chlorhexidine combined with Fluoride mouthwash and to measure its impact on plaque accumulation and on plaque pH. Initially 100 subjects were screened. A double blind, parallel randomized clinical trial was conducted on 30 subjects after applying inclusion and exclusion criteria. Other independent variables were matched before randomly allocating them in three groups: Group A-Chlorhexidine as positive control, Group B-Chlorhexidine + Fluoride as test group and Group C- Distilled water as negative control. Oral prophylaxis of participants was done before onset of the study. Plaque pH was assessed before and immediately after rinsing at 0, 5 and 10 minutes interval and after 7 days with digital pH electrode (pHepR pH meter, Hanna Instruments R10285) and accumulation of plaque was recorded by Turesky et al., modification of Quigley Hein Plaque Index (1970). ANOVA test was used for statistical analysis. Although there was a statistically significant reduction in mean plaque scores from baseline to seven days in both Groups A and B, Group B showed better anti-plaque efficacy . Almost equal drop in plaque pH was seen for both the groups at 5 and 10 minutes. Better anti-plaque efficacy was observed in Group B (Chlorhexidine and Fluoride combination) with minimum variation of plaque pH.

Molar-incisor hypomineralization (MIH): report of a severe case

The term molar-incisor hypomineralization describes an uncommon pathological entity characterized by the hypomineralization of permanent first molars and commonly affecting permanent incisors. In this paper we describe a case of a 9 years old female patient attended with severe dental sensibility, masticatory and phonetic difficulties. Clinical examination revealed severe enamel structural defects affecting all four permanent first molars and opaque stains affecting permanent incisors, besides caries lesions on posterior teeth and severe osseous basis discrepancy. Radiographic examination evidenced permanent first molars imbrication and shallow lesions on the enamel of these teeth. Therefore, molar-incisor hypomineralization was defined. After fluoride varnish applications, glass ionomer cement restorations and home performed fluoride mouthwash, patient continued to report dental sensibility. Molar-incisor hypomineralization is capable of led the affected patient through morbidity which results on quality of life decrease. Due to severe dentin exposure, therapeutic interventions aiming to reduce dental sensibility are not always effective.

The Effect of Fixed Orthodontic Appliances and Fluoride Mouthwash on the Oral Microbiome of Adolescents - A Randomized Controlled Clinical Trial.

While the aesthetic effect of orthodontic treatment is clear, the knowledge on how it influences the oral microbiota and the consequential effects on oral health are limited. In this randomized controlled clinical trial we investigated the changes introduced in the oral ecosystem, during and after orthodontic treatment with fixed appliances in combination with or without a fluoride mouthwash, of 10–16.8 year old individuals (N = 91). We followed several clinical parameters in time, in combination with microbiome changes using next-generation sequencing of the bacterial 16S rRNA gene. During the course of our study, the oral microbial community displayed remarkable resilience towards the disturbances it was presented with. The effects of the fluoride mouthwash on the microbial composition were trivial. More pronounced microbial changes were related to gingival health status, orthodontic treatment and time. Periodontal pathogens (e.g. Selenomonas and Porphyromonas) were highest in abundance during the orthodontic treatment, while the health associated Streptococcus, Rothia and Haemophilus gained abundance towards the end and after the orthodontic treatment. Only minor compositional changes remained in the oral microbiome after the end of treatment. We conclude that, provided proper oral hygiene is maintained, changes in the oral microbiome composition resulting from orthodontic treatment are minimal and do not negatively affect oral health.

Effectiveness of probiotic, chlorhexidine and fluoride mouthwash against Streptococcus mutans - Randomized, single-blind, in vivo study.

Aim:To determine the short-term efficiency of probiotic, chlorhexidine, and fluoride mouthwashes on plaque Streptococcus mutans level at four periodic intervals.Materials and Methods:This was a single-blind, randomized control study in which each subject was tested with only one mouthwash regimen. Fifty-two healthy qualified adult patients were selected randomly for the study and were divided into the following groups: group 1- 10 ml of distilled water, group 2- 10 ml of 0.2% chlorhexidine mouthwash, group 3- 10 ml of 500 ppm F/400 ml sodium fluoride mouthwash, and group 4- 10 ml of probiotic mouthwash. Plaque samples were collected from the buccal surface of premolars and molars in the maxillary quadrant. Sampling procedure was carried out by a single examiner after 7 days, 14 days, and 30 days, respectively, after the use of the mouthwash. All the samples were subjected to microbiological analysis and statistically analyzed with one-way analysis of variance (ANOVA) and post-hoc test.Results:One-way ANOVA comparison among groups 2, 3, and 4 showed no statistical significance, whereas group 1 showed statistically significant difference when compared with groups 2, 3, and 4 at 7th, 14th, and 30th day.Conclusion:Chlorhexidine, sodium fluoride, and probiotic mouthwashes reduce plaque S. mutans levels. Probiotic mouthwash is effective and equivalent to chlorhexidine and sodium fluoride mouthwashes. Thus, probiotic mouthwash can also be considered as an effective oral hygiene regimen.

Comparative Evaluation of the Effects of Fluoride Mouthrinse, Herbal Mouthrinse and Oil Pulling on the Caries Activity and Streptococcus mutans Count using Oratest and Dentocult SM Strip Mutans Kit.

ABSTRACTBackground: As the technological level of healthcare increases, it is important not to lose sight of the basics of patient care. No matter how sophisticated dental techniques have become, preventive dentistry still remains the foundation for oral health. Therefore, antimicrobial mouthrinses are developed to provide an effective means of preventing colonization by micro-organisms.Aim: The aim of this study was to evaluate and compare the antimicrobial activity of oil pulling, herbal mouthrinses and fluoride mouthwash on the caries activity and S. mutans counts in the saliva of children, using Oratest and Dentocult SM kit.Design: Fifty-two healthy children between the age group of 6 to 12 years were selected for the study and divided into four groups based on the mouthrinse used as group 1: fluoride, group 2: herbal, group 3: oil pulling and group 4: control. The estimation of caries activity and S. mutans was done prior to and after the subjects were instructed to use the mouthrinse twice daily for a period of 2 weeks.Statistical analysis: The comparisons were made by applying paired ‘t’ test with the level of significance set at p < 0.05. Difference between more than two mean values was done by using ANOVA and Post hoc Bonferroni test was used for multiple comparisons.Results and conclusion: The efficacy of fluoride and herbal mouthrinses was found to be comparable while oil pulling did not provide any additional benefit to be used as an effective antimicrobial agent in reducing the bacterial colonization of an individual.How to cite this article: Jauhari D, Srivastava N, Rana V, Chandna P. Comparative Evaluation of the Effects of Fluoride Mouthrinse, Herbal Mouthrinse and Oil Pulling on the Caries Activity and Streptococcus mutans Count using Oratest and Dentocult SM Strip Mutans Kit. Int J Clin Pediatr Dent 2015;8(2):114-118.

Galvanic Corrosion of and Ion Release from Various Orthodontic Brackets and Wires in a Fluoride-containing Mouthwash.

Background and aims. This study compared the galvanic corrosion of orthodontic wires and brackets from various manufacturers following exposure to a fluoride mouthwash.Materials and methods. This study was conducted on 24 lower central incisor 0.022" Roth brackets of four different commercially available brands (Dentaurum, American Orthodontics, ORJ, Shinye). These brackets along with stainless steel (SS) or nickel-titanium (NiTi) orthodontic wires (0.016", round) were immersed in Oral-B mouthwash containing 0.05% sodium fluoride for 28 days. The electric potential (EP) difference of each bracket-wire couple was measured with a Saturated Calomel Reference Electrode (Ag/AgCl saturated with KCl) via a voltmeter. The ions released in the electrolyte weremeasured with an atomic absorption spectrometer. All the specimens were assessed under a stereomicroscope and specimens with corrosion were analyzed with scanning electron microscopy (SEM). Data were analyzed using ANOVA.Results. The copper ions released from specimens with NiTi wire were greater than those of samples containing SS wire. ORJ brackets released more Cu ions than other samples. The Ni ions released from Shinye brackets were significantly more than those of other specimens (P < 0.05). Corrosion rate of brackets coupled with NiTi wires was higher than that of brackets coupled with SS wires. Light and electron microscopic observations showed greater corrosion of ORJ brackets.Conclusion. In fluoride mouthwash, Shinye and ORJ brackets exhibited greater corrosion than Dentaurum and American Orthodontics brackets. Stainless steel brackets used with NiTi wires showed greater corrosion and thus caution is recommended when using them.

The effect of different environmental factors on force degradation of three common systems of orthodontic space closure.

Different environmental conditions, such as high temperature or exposure to some chemical agents, may affect the force decay of different methods of space closure during orthodontic treatment. The aim of this in vitro study was to evaluate the force decay pattern in the presence of tea as a popular drink in some parts of the world and two mouthwashes that are usually prescribed by the orthodontist once the treatment is in progress. Elastic chain (EC), nickel-titanium (Ni-Ti) closed coil spring and tie-back (TB) method were used as the means of space closure. The specimens were placed in five different media: Hot tea, hot water (65°), chlorhexidine mouthwash, fluoride mouthwash and the control group (water at 37°). The specimens were stretched 25 mm and the elastic force of three systems was measured at the beginning of the study, after 24 h, after 1 week and after 3 weeks. One-way ANOVA was used to compare the results between the groups and Duncan test was carried out to compare the sets of means in different groups (P ≤ 0.05). Tea increases the force decay in the EC and TB groups. Oral mouthwashes also resulted in more rapid force decay than the control group. EC and Ni-Ti groups were not much affected in the presence of oral mouthwashes. Regarding the immersion media, TB method showed the biggest variation in different media and Ni-Ti coil spring was least affected by the type of media.

An in vitro assessment of the mechanical characteristics of nickel-titanium orthodontic wires in Fluoride solutions with different acidities.

Objectives:The aim was to evaluate the in vitro effects of fluoride solutions with different acidities on load-deflection characteristics of nickel-titanium (NiTi) orthodontic wires.Materials and Methods:In this study, which lasted 30 days, 36 (3 cm long, 0.016 × 0.022 inches, SENT 1622, G & H wire Company, Greenwood, Indiana, USA) NiTi wires, were divided into three experimental groups of 12 each. Two groups were subjected to 0.05 topical fluoride mouthwash with different acidities (G1, pH 4; G2, pH 6.6) for 90 s, twice a day, and kept in normal saline after that. The third group (G3, the control group) was kept in normal saline only. Load and unload forces were measured with three bracket bending test in a universal testing machine (Testometric Co, Rochdale, UK). Loading and unloading plateaus and hysteresis were also recorded. Data were then analyzed using analysis of variance and honestly significant difference Tukey at P < 0.05.Results:During the loading phase, there was a significant difference between deflections (P < 0.001); but there was no interaction effect (P = 0.191) and no significant difference among three groups (P = 0.268). In the unloading phase, there was a significant difference between deflections (P < 0.001) and an interaction effect was also observed (P = 0.008). Further, significant differences noted among three groups (P = 0.037). Only in the unloading phase, at deflections of 2.2 through 0.2 mm, significant differences between the mean force values of the G1 and G3 groups were observed (P = 0.037).Conclusion:Based on this in-vitro study, compared to neutral fluoride solution, daily mouthwash with a fluoride solution with more acidic pH of 4 affected the NiTi wires load-deflection characteristics during the unloading phase. This finding may have clinical implications and can be further validated by in-vivo studies.

White spot lesions: prevention and management during the orthodontic treatment.

The formation of white spot lesions, or enamel demineralization, around fixed orthodontic attachments is a common complication during and following fixed orthodontic treatment, which marks the result of a successfully completed case. This article is a contemporary review of the risk factors and preventive methods of these orthodontics scars. Preventive programmes must be emphasized to all orthodontic patients. The responsibility of an orthodontist is to minimize the risk of the patient having decalcification as a consequence of orthodontic treatment by educating and motivating the patients for excellent oral hygiene practice. Prophylaxis with topical fluoride application should be implemented: high-fluoride toothpastes, fluoride mouthwashes, gels and varnishes during and after the orthodontic treatment, especially for patients at high risk of caries.

Oral Health Behaviors and Dental Treatment During Pregnancy: A Cross-Sectional Study Nested in a Cohort in Northeast Brazil

Objective: To evaluate changes in oral health behavior and the use of dental health services during pregnancy. Material and Methods: This is a cross-sectional study nested in a prospective cohort of pregnant women in the city of Sao Luis, MA. A total of 262 women receiving prenatal care at the Mother-Infant University Hospital were interviewed, through a semi-structured questionnaire, regarding their use of dental services, type of dental treatment received, dietary habits, and oral hygiene before and during pregnancy. The Wilcoxon and McNemar tests (alpha=1%) were used to evaluate the differences in behavior before and during pregnancy. Results: Of the pregnant women evaluated, 33 women (12.6%) reported undergoing at least one dental consultation during their pregnancy. Of these women, 30 (11.4%) had at least one consultation during their first trimester, 10 (3.8%) during their second trimester, and 2 (0.8%) during their third trimester. Preventative and restorative treatments were the most common treatments. There were differences in the behaviors of women related to oral health before and during pregnancy. Subjects reported less frequent daily and post-meal tooth brushing and weekly dental floss and fluoridated mouthwash use during pregnancy compared to prepregnancy (p<0.001). They also reported eating more snacks (p<0.001) and meals (p=0.011) per day during pregnancy compared to prepregnancy. Only the habit of regular sugary food consumption did not vary significantly (p=0.169). Conclusion: There are behavioral changes during pregnancy that can cause dental caries and periodontal disease. Despite these concerns, dental health professionals are not adequately caring for pregnant women. Therefore, public policies incentivizing prenatal dental care are needed.

Effectiveness of a New Generation Anticavity Mouthwash on Streptococcus mutans and Lactobacillus acidophilus Count: A Microbiological Study

ABSTRACT Background Dental caries is one of the most widespread disease. CariFree CTx4 treatment rinse mouthwash is designed to treat the cavity-causing plaque biofilm, reduce the overpopulation of cavity-causing bacteria, and neutralize decaycausing acids with patent-pending pH+ technology. Aim The aim of the present study was to assess and compare the antibacterial effect of a new generation anticavity mouthwash (CariFree CTx4 treatment rinse) with that of a 0.2% chlorhexidine mouthwash (Clohex), 0.05% sodium fluoride mouthwash (Colgate Plax Complete Care), 2% povidone iodine mouthwash (Betadine gargle and mouthwash) against Streptococcus mutans and Lactobacillus acidophilus. Materials and methods Strains of S. mutans and L. acidophilus were grown on suitable media and the inhibitory effect of the test substances was noted by disk diffusion method and Agar well diffusion method. The results obtained were then subjected to statistical analysis. Result Efficacy of CTx4 treatment rinse mouthwash was less than chlorhexidine for both S. mutans and L. acidophilus whereas its efficacy was comparable to that of povidone iodine and sodium fluoride mouthwash for S. mutans and to that of sodium fluoride for L. acidophilus. Conclusion It was concluded that chlorhexidine is still the best mouthwash available. How to cite this article Venkataraghavan K, Shah S, Choudhary P, Kaur M, Shah S, Trivedi K. Effectiveness of a New Generation Anticavity Mouthwash on Streptococcus mutans and Lactobacillus acidophilus Count: A Microbiological Study. World J Dent 2014;5(3):152-156.

The effect of casein phosphopeptide-amorphous calcium phosphate paste and sodium fluoride mouthwash on the prevention of dentine erosion: An in vitro study.

Aim:The purpose was to compare the effect of 0.2% sodium fluoride mouthwash and casein phosphopeptide-amorphous calcium phosphate paste on prevention of dentin erosion.Materials and Methods:Buccal surfaces of 36 sound premolar teeth were ground flat and polished with abrasive discs. Half the polished surfaces were covered with tape to maintain a reference surface. Samples were randomly allocated into three groups. Group A was pretreated with tooth mousse (TM) 4 times a day for 5 days. Group B was pretreated with 0.2% sodium fluoride mouthwash 4 times a day for 5 days. Group C was considered as the control group with no pretreatment. In the next step, the samples were exposed to Coca-Cola 4 times a day for 3 days. After each erosive cycle, the samples were rinsed with deionized water and stored in artificial saliva. The surface loss was determined using profilometry.Results:The erosion in both Groups A and B was less than the control group. The surface loss in mouthwash group was significantly lower than in the control group. Erosion in TM group was more than the mouthwash group and less than the control group.Conclusion:Sodium fluoride mouthwash is more effective for prevention of dentin erosion.

Influence of fluoride mouthwash containing nanohydroxyapatite on dentin interface microhardness

Influence of fluoride mouthwash containing nanohydroxyapatite on dentin interface microhardness

&lt;b&gt;Evaluation of simplified oral hygiene index of the elementary school students before fluoride mouthwash&lt;/b&gt; - doi: 10.4025/actascihealthsci.v35i2.13205

The State Program of fluoride mouthwashes for caries control was established in 1980 in elementary schools of Paraná State covering children 7-11 years old. Knowing the importance of removing bacterial plaque to reach the maximum desired effect of prevention, this study aimed to evaluate the Simplified Oral Hygiene Index, before applying the solution of Sodium Fluoride (NaF; 0.2%) in children from an elementary school in the city of Nova Aurora, Paraná State, by using disclosing dental plaque. This is a quantitative research, descriptive and exploratory whose data were obtained through a specific form, with 61 children and analyzed using descriptive statistics with distribution of absolute and percentage frequencies. Most children (60%) showed the worst results - regular and bad - with presence of plaque and risk of dental caries. Therefore, we should establish a prevention program in oral health that must involve parents and students. This program should be developed by health professionals inside the school, explaining about the etiologic factors, causes and consequences of plaque, the techniques of cleaning and maintenance of hygiene instruments, and the risks of the lack of proper hygiene in the oral cavity.

Efficacy of a mouthwash containing 0.8% arginine, PVM/MA copolymer, pyrophosphates, and 0.05% sodium fluoride compared to a commercial mouthwash containing 2.4% potassium nitrate and 0.022% sodium fluoride and a control mouthwash containing 0.05% sodium fluoride on dentine hypersensitivity: A six-week randomized clinical study

ObjectiveEvaluate the efficacy of 0.8% arginine, potassium nitrate and sodium fluoride mouthwashes on dentine hypersensitivity reduction. MethodsSix week randomized, double blinded, two cell, parallel single centre clinical study in the Dominican Republic; subjects were randomized into three treatment groups: mouthwash containing 0.8% arginine, PVM/MA copolymer, pyrophosphates, and 0.05% sodium fluoride in an alcohol-free base (arginine); mouthwash containing 2.4% potassium nitrate and 0.022% sodium fluoride (potassium nitrate); a control mouthwash containing 0.05% sodium fluoride (negative control). Tactile and air-blast dentine hypersensitivity assessments were conducted at baseline, thirty minutes post rinsing and two, four, and six weeks of twice-daily product use. For treatment group comparisons, ANCOVA and post hoc Tukey's pair-wise comparisons (α=0.05) were done. ResultsSeventy-five subjects were enrolled; 69 subjects completed the study. There were no differences after thirty minutes of a single use, among the three groups with respect to mean tactile and air blast hypersensitivity scores compared to potassium nitrate and negative control mouthwashes (p<0.05). The arginine group presented a statistically significant improvement in the mean tactile scores compared to potassium nitrate and negative control groups after two, four, and six weeks (p<0.001) of product use; the arginine group showed a statistically significant enhancement in air blast hypersensitivity mean scores compared to potassium nitrate and negative control groups after two (p=0.001), four (p<0.001), and six weeks (p<0.001) of product use. ConclusionA mouthwash containing arginine provides a significant and superior reduction in dentine hypersensitivity compared to potassium nitrate and a negative control mouthwash after two weeks.

The effect of fluoride exposure on the load-deflection properties of superelastic nickel-titanium-based orthodontic archwires

Abstract Background: It has been demonstrated that fluoride prophylactic agents may cause hydrogen absorption in NiTi wires and degrade their mechanical properties. Aims: To investigate the effect of a fluoride mouthwash on load-deflection characteristics of three types of nickel-titanium-based orthodontic archwires. Methods: Twenty maxillary 0.016 inch round specimens from each of the single-strand NiTi (Rematitan ‘Lite’), multi-strand NiTi (SPEED Supercable) and Copper NiTi (Damon Copper NiTi) wires were selected. The specimens were kept in either 0.2% NaF or artificial saliva solutions at 37°C for 24 hours (N = 10). The wire load-deflection properties were measured by a Zwick testing machine, using a three-point bending test. An un-paired student’s t-test, a one-way ANOVA and a Tukey post-hoc test were used to assess statistical significance. Results: Immersion in NaF solution affected the load-deflection properties of NiTi wires. The unloading forces at 0.5 and 1.0 mm deflections were significantly lower in fluoride-treated specimens compared with the control groups (p &lt; 0.05). Unloading forces at 1.5, 2.0 and 2.5 mm deflections were not statistically different between fluoride- and saliva-treated specimens (p &gt; 0.05). Conclusions: The results suggested that subjecting NiTi wires to fluoride agents decreased associated unloading forces, especially at lower deflections, and may result in delayed tooth alignment.

Effect of chlorhexidine mouth wash and fluoridated mouth wash on mechanical properties of orthodontic arch wires (An in vitro study)

Effect of chlorhexidine mouth wash and fluoridated mouth wash on mechanical properties of orthodontic arch wires (An in vitro study)

CORROSION OF AMALGAMS IN ORAL CAVITY

This paper is a study of the effect of natural saliva (oral cavity) and a fluoride mouthwash on dentalamalgams .Two types electrodes were made the first was of a high copper amalgam while the second wasmade from a low copper amalgam. They were immersed in two types of electrolytes for twelve hours and the whole galvanic cell was connected to a computer via a potentiosat. Their corrosion currents, corrosionvoltage and corrosion resistance was recorded and compared to find which medium that is usually has themost severe effect on the amalgams corrosion.

Effects of Chlorhexidine and Fluoride on Irradiated Enamel and Dentin

The effectiveness of mouthwash protocols in preventing gamma irradiation therapy damage to the ultimate tensile strength (UTS) of enamel and dentin is unknown. It was hypothesized that the use of chlorhexidine and fluoride mouthwash would maintain the UTS of dental structures. One hundred and twenty teeth were divided into 2 groups: irradiated (subjected to 60 Gy of gamma irradiation in daily increments of 2 Gy) and non-irradiated. They were then subdivided into 2 mouthwash protocols used 3 times per day: 0.12% chlorhexidine, 0.05% sodium fluoride, and control group (n = 10). The specimens were evaluated by microtensile testing. The results of the Tukey test (p < 0.05) indicated that the gamma irradiation therapy significantly reduced the UTS of the enamel, crown, and root dentin. Macromolecular alterations were suggested by optical retardation data in dentin. Structural alterations, in both substrates, were detected by scanning electron microscopy analysis. Mouthwash with 0.12% chlorhexidine partially prevented the damage to the mechanical properties of the irradiated crown dentin, whereas the 0.05% sodium-fluoride-irradiated enamel showed UTS similar to that of non-irradiated enamel.