Fluidic Network Research Articles

Microvalves for Applications in Centrifugal Microfluidics.

Centrifugal microfluidic platforms (CDs) have opened new possibilities for inexpensive point-of-care (POC) diagnostics. They are now widely used in applications requiring polymerase chain reaction steps, blood plasma separation, serial dilutions, and many other diagnostic processes. CD microfluidic devices allow a variety of complex processes to transfer onto the small disc platform that previously were carried out by individual expensive laboratory equipment requiring trained personnel. The portability, ease of operation, integration, and robustness of the CD fluidic platforms requires simple, reliable, and scalable designs to control the flow of fluids. Valves play a vital role in opening/closing of microfluidic channels to enable a precise control of the flow of fluids on a centrifugal platform. Valving systems are also critical in isolating chambers from the rest of a fluidic network at required times, in effectively directing the reagents to the target location, in serial dilutions, and in integration of multiple other processes on a single CD. In this paper, we review the various available fluidic valving systems, discuss their working principles, and evaluate their compatibility with CD fluidic platforms. We categorize the presented valving systems into either "active", "passive", or "hybrid"-based on their actuation mechanism that can be mechanical, thermal, hydrophobic/hydrophilic, solubility-based, phase-change, and others. Important topics such as their actuation mechanism, governing physics, variability of performance, necessary disc spin rate for valve actuation, valve response time, and other parameters are discussed. The applicability of some types of valves for specialized functions such as reagent storage, flow control, and other applications is summarized.

Applications of Magneto Electrochemistry and Magnetohydrodynamics in Microfluidics

Magnetic fields affect electrolytes in diverse ways. This paper focuses on the interactions among electric, magnetic, and flow fields and the applications of the resulting phenomena in microfluidics. When an electrical current is transmitted in an electrolyte in the presence of an external magnetic field, a Lorentz body force results, which may induce pressure gradients and fluid motion—magnetohydrodynamics (MHD). The resulting advection is used to pump fluids, induce/suppress flow instabilities, and control mass transfer in diverse electrochemical processes. When an electrolyte flows in the presence of a magnetic field, electromotive force (emf) is induced in the electrolyte and can be used for flow metering, hydrogen production, and energy conversion. This review describes the governing equations for modeling MHD flows in electrolytes and MHD phenomena and applications relevant to microfluidic systems, such as the use of MHD to pump and stir fluids, propel swimmers, and control fluid flow in fluidic networks without any mechanical components. The paper also briefly assesses the impact of magnetic resonance imaging (MRI) on blood flow. MHD in electrolytes is a highly interdisciplinary, combining electrokinetics, fluid mechanics, electrochemistry, and Maxwell equations.

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix

Blood stream infections (BSIs) cause high mortality, and their rapid detection remains a significant diagnostic challenge. Timely and informed administration of antibiotics can significantly improve patient outcomes. However, blood culture, which takes up to 5 d for a negative result, followed by PCR remains the gold standard in diagnosing BSI. Here, we introduce a new approach to blood-based diagnostics where large blood volumes can be rapidly dried, resulting in inactivation of the inhibitory components in blood. Further thermal treatments then generate a physical microscale and nanoscale fluidic network inside the dried matrix to allow access to target nucleic acid. The amplification enzymes and primers initiate the reaction within the dried blood matrix through these networks, precluding any need for conventional nucleic acid purification. High heme background is confined to the solid phase, while amplicons are enriched in the clear supernatant (liquid phase), giving fluorescence change comparable to purified DNA reactions. We demonstrate single-molecule sensitivity using a loop-mediated isothermal amplification reaction in our platform and detect a broad spectrum of pathogens, including gram-positive methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria, gram-negative Escherichia coli bacteria, and Candida albicans (fungus) from whole blood with a limit of detection (LOD) of 1.2 colony-forming units (CFU)/mL from 0.8 to 1 mL of starting blood volume. We validated our assay using 63 clinical samples (100% sensitivity and specificity) and significantly reduced sample-to-result time from over 20 h to <2.5 h. The reduction in instrumentation complexity and costs compared to blood culture and alternate molecular diagnostic platforms can have broad applications in healthcare systems in developed world and resource-limited settings.

Development and validation of a variational formulation of Two-Phase flow distribution

Nonuniform flow distribution in vertical headers has been an ongoing challenge that limits the advantages of transfer and fluidic technologies, especially in microchannel heat exchangers (MCHXs). This issue has been typically addressed in empirical studies and case-specific computational fluid dynamics (CFD) simulations; however, efforts toward the theoretical understanding and modeling of this phenomenon are lacking in literature. Therefore, this paper presents the development and validation of a theoretical model to reproduce the flow and phase distributions in adiabatic multibranch channels. A variational formulation of the phenomenon is obtained using Prigogine's theorem of minimum entropy production to cope with the inherent high variability of the mathematical problem. The individual flow rates, vapor quality, and pressure distributions yielding the minimum entropy generation rate provide a complete representation of the steady-state fluidic network for an arbitrary structure of the device and any working fluid. This paper presents a first experimental validation of the model when applied to the vertical header of a MCHX circulating R410A refrigerant. The theoretical results provide a coherent representation of the experimental data.

Characteristics of Oscillation in Cavity of Helmholtz Nozzle Generating Self-excited Pulsed Waterjet

Cavity flow oscillations in the axisymmetric cavity are critical to the operating efficiency of self-excited pulsed waterjets, which are widely employed in many practical applications. In this study, the behaviors of a turbulent flow in axisymmetric cavities causing cavity flow oscillations are investigated based on wall pressure characteristics. Experiments are performed using four Helmholtz nozzles with varying length-to-radius ratios at flow velocities of 20–80 m/s. Three orders of hydrodynamic modes in axisymmetric cavity are obtained through the spectral analysis of wall pressure. Based on the experimental results, the empirical coefficient of Rossiter’s formula is modified, and the values of the parameter phase lag and the ratio of convection velocity to free stream velocity are obtained as 0.061 and 0.511, respectively. In addition, the spectral peak with a relatively constant frequency shows that the flow-acoustic resonance is excited significantly. A modified model is introduced based on the fluidic networks to predict the lock-on frequency. The results obtained can provide a basis for the structural optimization of the nozzle to improve the performance of self-excited pulsed waterjets.

A survey of 3D printing technology applied to paper microfluidics.

Paper microfluidics is a rapidly growing subfield of microfluidics in which paper-like porous materials are used to create analytical devices that are well-suited for use in field applications. 3D printing technology has the potential to positively affect paper microfluidic device development by enabling tools and methods for the creation of devices with well-defined and tunable fluidic networks of porous matrices for high performance signal generation. This critical review focuses on the progress that has been made in using 3D printing technologies to advance the development of paper microfluidic devices. We describe printing work in three general categories: (i) solid support structures for paper microfluidic device components; (ii) channel barrier definition in existing porous materials; and (iii) porous channels for capillary flow, and discuss their value in advancing paper microfluidic device development. Finally, we discuss major areas of focus for highest impact on the next generation of paper microfluidics devices.

Design and Fabrication of Multichannel PDMS Microfluidic

Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC’s. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.

Label-Free Identification of Single Mononucleotides by Nanoscale Electrophoresis.

Nanoscale electrophoresis allows for unique separations of single molecules, such as DNA/RNA nucleobases, and thus has the potential to be used as single molecular sensors for exonuclease sequencing. For this to be envisioned, label-free detection of the nucleotides to determine their electrophoretic mobility (i.e., time-of-flight, TOF) for highly accurate identification must be realized. Here, for the first time a novel nanosensor is shown that allows discriminating four 2-deoxyribonucleoside 5'-monophosphates, dNMPs, molecules in a label-free manner by nanoscale electrophoresis. This is made possible by positioning two sub-10nm in-plane pores at both ends of a nanochannel column used for nanoscale electrophoresis and measuring the longitudinal transient current during translocation of the molecules. The dual nanopore TOF sensor with 0.5, 1, and 5µm long nanochannel column lengths discriminates different dNMPs with a mean accuracy of 55, 66, and 94%, respectively. This nanosensor format can broadly be applicable to label-free detection and discrimination of other single molecules, vesicles, and particles by changing the dimensions of the nanochannel column and in-plane nanopores and integrating different pre- and postprocessing units to the nanosensor. This is simple to accomplish because the nanosensor is contained within a fluidic network made in plastic via replication.

Lab-on-a-Disc for Point-of-Care Infection Diagnostics

Reliable, inexpensive, and rapid diagnostic tools are essential to control and prevent the spread of infectious diseases. Many commercial kits for coronavirus disease 2019 (COVID-19) diagnostics have played a crucial role in the fight against the COVID-19 pandemic. Most current standard in vitro diagnostic (IVD) protocols for infectious diseases are sensitive but time-consuming and require sophisticated laboratory equipment and specially trained personnel. Recent advances in biosensor technology suggest the potential to deliver point-of-care (POC) diagnostics that are affordable and provide accurate results in a short time. The ideal "sample-in-answer-out" type fully integrated POC infection diagnostic platforms are expected to be autonomous or easy-to-operate, equipment-free or infrastructure-independent, and high-throughput or easy to upscale. In this Account, we detail the recent progress made by our group and others in the development of centrifugal microfluidic devices or lab-on-a-disc (LOAD) systems. Unlike conventional pump-based fluid actuation, the centrifugal force generated by spinning the disc induces liquid pumping and no external fluidic interconnects are required. This allows a total fluidic network required for multiple steps of biological assays to be integrated on a disc, enabling fully automated POC diagnostics. Various applications have been demonstrated, including liquid biopsy for personalized cancer management, food applications, and environmental monitoring; here, we focus on IVD for infectious disease. First, we introduce various on-disc unit operation technologies, including reagent storage, sedimentation, filtration, valving, decanting, aliquoting, mixing, separation, serial dilution, washing, and calibration. Such centrifugal microfluidic technologies have already proved promising for micro-total-analysis systems for automated IVD ranging from molecular detection of pathogens to multiplexed enzyme-linked immunosorbent assays (ELISAs) that use raw samples such as whole blood or saliva. Some recent examples of LOAD systems for molecular diagnostics in which some or all steps of the assays are integrated on a disc, including pathogen enrichment, nucleic acid extraction, amplification, and detection, are discussed in detail. We then introduce fully automated ELISA systems with enhanced sensitivity. Furthermore, we demonstrate a toy-inspired fidget spinner that enables electricity-free and rapid analysis of pathogens from undiluted urine samples of patients with urinary tract infection symptoms and a phenotypic antimicrobial susceptibility test for an extreme POC diagnostics application. Considering the urgent need for cost-effective and reliable POC infection diagnostic tools, especially in the current pandemic crisis, the current limitations and future directions of fast and broad adaptation in real-world settings are also discussed. With proper attention to key challenges and leverage with recent advances in bio-sensing technologies, molecular biology, nanomaterials, analytical chemistry, miniaturization, system integration, and data management, LOAD systems hold the potential to deliver POC infection diagnostic tools with unprecedented performance regarding time, accuracy, and cost. We hope the new insight and promise of LOAD systems for POC infection diagnostics presented in this Account can spark new ideas and inspire further research and development to create better healthcare systems for current and future pandemics.

Variational formulation of stationary two-phase flow distribution

This paper discusses the extremisation of the entropy production in fluidic networks to gain insight into using non-equilibrium thermodynamics for mathematically formulating thermal engineering transfer phenomena. In the study, a variational formulation of the two-phase flow distribution in a fluidic junction is developed. Moreover, based on the flow representation, it is shown that the flow is distributed such that the entropy production rate is either maximised or minimised. Specifically, considering a homogeneous representation of the flow in an adiabatic fluidic network, the flow rates are distributed to maximize the entropy generation rate. Contrarily, when a separate flow representation is adopted, the stationary flow rates are distributed to yield the minimum entropy generation rate. Additionally, various characteristics which affect the flow distribution ratio and phase separation, such as the geometric imbalance, the gravitational static head, the total inlet mass flux, different thermo-physical properties of the fluid, and a generalised number of parallel branches are explored. This supports the possibility of relying on non-equilibrium thermodynamics, instead of introducing case-specific empirical correlations, for obtaining general mathematical formulations of thermal engineering transfer phenomena.

System Modularity Chip for Analysis of Rare Targets (SMART-Chip): Liquid Biopsy Samples.

Liquid biopsies are becoming popular for managing a variety of diseases due to the minimally invasive nature of their acquisition, thus potentially providing better outcomes for patients. Circulating tumor cells (CTCs) are among the many different biomarkers secured from a liquid biopsy, and a number of efficient platforms for their isolation and enrichment from blood have been reported. However, many of these platforms require manual sample handling, which can generate difficulties when translating CTC assays into the clinic due to potential sample loss, contamination, and the need for highly specialized operators. We report a system modularity chip for the analysis of rare targets (SMART-Chip) composed of three task-specific modules that can fully automate processing of CTCs. The modules were used for affinity selection of the CTCs from peripheral blood with subsequent photorelease, simultaneous counting, and viability determinations of the CTCs and staining/imaging of the CTCs for immunophenotyping. The modules were interconnected to a fluidic motherboard populated with valves, interconnects, pneumatic control channels, and a fluidic network. The SMART-Chip components were made from thermoplastics via microreplication, which lowers the cost of production making it amenable to clinical implementation. The utility of the SMART-Chip was demonstrated by processing blood samples secured from colorectal cancer (CRC) and pancreatic ductal adenocarcinoma (PDAC) patients. We were able to affinity-select EpCAM expressing CTCs with high purity (0-3 white blood cells/mL of blood), enumerate the selected cells, determine their viability, and immunophenotype the cells. The assay could be completed in <4 h, while manual processing required >8 h.

A microfluidic single-cell array for in situ laminar-flow-based comparative culturing of budding yeast cells

To facilitate in situ comparative culturing of budding yeast cells in a precisely controlled microenvironment, we developed a microfluidic single-cell array (MiSCA) with 96 traps (16 rows × 6 columns) for single-cell immobilization. Through optimization of the distances between neighboring traps and the applied flow rates by using a hydraulic equivalent circuit of the fluidic network, yeast cells were delivered to each column of the array by laminar focused flows and reliably captured at the traps by hydrodynamic forces with about 90% efficiency of cell immobilization. Immobilized cells in different columns within the same device can then be cultured in parallel while being exposed to different media and compounds delivered by laminar flows. For biological validation of the comparative cell-culturing device, we used budding yeast that can express yellow fluorescent protein upon the addition of β-estradiol in cell-culturing medium. Experimental results show successful induction of fluorescence in cells immobilized in desired columns that have been dosed with β-estradiol. The MiSCA system allows for performing sets of experiments and control experiments in parallel in the same device, or for executing comparative experiments under well-defined laminar-perfusion conditions with different media, as well as in situ monitoring of dynamic cellular responses upon different analytical compounds or reagents for single-cell analysis.

Flow Rectification in Loopy Network Models of Bird Lungs.

We demonstrate flow rectification, valveless pumping, or alternating to direct current (AC-to-DC) conversion in macroscale fluidic networks with loops. Inspired by the unique anatomy of bird lungs and the phenomenon of directed airflow throughout the respiration cycle, we hypothesize, test, and validate that multiloop networks exhibit persistent circulation or DC flows when subject to oscillatory or AC forcing at high Reynolds numbers. Experiments reveal that disproportionately stronger circulation is generated for higher frequencies and amplitudes of the imposed oscillations, and this nonlinear response is corroborated by numerical simulations. Visualizations show that flow separation and vortex shedding at network junctions serve the valving function of directing current with appropriate timing in the oscillation cycle. These findings suggest strategies for controlling inertial flows through network topology and junction connectivity.

Paper-based multiplex analytical device for simultaneous detection of Clostridioides difficile toxins and glutamate dehydrogenase

We report a new paper-based multiplex analytical device (mPAD) for simultaneous screening of three analytes (glutamate dehydrogenase, toxin A, and toxin B) known as biomarkers for Clostridioides difficile infection (CDI). To overcome the limitation of common rapid assays (e.g. lateral flow immunochromatographic and enzyme immunoassays) in terms of multiplexing, sensitivity, simplicity, and ease-of-use, the mPAD is constructed with a three dimensional (3D) configuration of paper components with a multi-channel design. Multiple fluidic paths developed with wax-patterned paper allow the simultaneous detection of glutamate dehydrogenase, toxin A, and toxin B without any cross-reactivity. The 3D fluidic network on the mPAD facilitates a self-operating test procedure for the mixing and addition of amplification reagents with a one-step sliding operation. The results of the multiplex CDI assay can be easily interpreted by the naked eye within 10 min, and are visually intensified over time resulting in up to 3-fold signal amplification. Our device exhibited remarkable analytical performances for the simultaneous detection of three CDI biomarkers, providing a sensitivity of 97%, specificity of 88%, accuracy of 95%, and limits of detection for glutamate dehydrogenase, toxin A, and toxin B of 0.16 ng mL−1, 0.09 ng mL−1, and 0.03 ng mL−1, respectively. These results indicate the high applicability and feasibility of mPAD for multiplex testing for CDI with the advantages of being simple, sensitive, inexpensive, user-friendly, and equipment-free.

Wirelessly-controlled electrolysis pumps on lab-on-a-disc for automation of bioanalytical assays

Electrolysis pumping on microfluidic CDs relies on pneumatic pressure to propel liquids against the direction of centrifugal force. This active pumping method operates in a closed fluidic network, which prevents introducing contaminations from the environment to the CD. However, due to two main reasons, the conventional pump has not been suitable for the automation of complex bioanalytical assays. First, the layout of the electrolysis pump requires a large space of the disc. Second, the slip-rings used for pump operation have a short number of connection ports and a short shelf-life due to mechanical wear. To overcome these challenges, we developed a compact 3D and wirelessly-controlled electrolysis pump. Compared to the conventional electrolysis pump, the 3D device allows for better use of the limited disc space, and therefore the integration of more pumping units and/or other fluidic components. Our 3D electrolysis pump is electrically supplied by an “electrified Lab-on-a-Disc” board that enables wireless control of several pumps, as well as other actuators or sensors, on spinning CDs. We developed an android application to control the activation of several electrolysis pumps simultaneously. To evaluate the liquid pumping with the proposed design, we recorded the time required to pump various liquid volumes from 20 to 120 µL. The EL pump showed repetitive behavior in terms of the time needed to displace specific volumes. As a proof of concept of the use of the pump in an application, we developed a centrifugal microfluidic device with several pumps and an active valve to automate the fluidic steps commonly used in an immunoassay with peptide-microarrays.

A microfluidically controlled concave\u2013convex membrane lens using an addressing operation system

Electrical control toolkits for microlens arrays are available to some extent, but for applications in environments with strong electromagnetic fields, radiation, or deep water, non-electrical actuation and control strategies are more appropriate. An integrated digital microfluidic zoom actuating unit with a logic addressing unit for a built-in membrane lens array, e.g., a flexible bionic compound eye, is developed and studied in this article. A concave–convex membrane fluidic microvalve, which is the component element of the logic gate, actuator, and microlens, is proposed to replace the traditional solenoid valve. The functions of pressure regulation and decoding can be obtained by incorporating microvalves into fluidic networks according to equivalent circuit designs. The zoom actuating unit contains a pressure regulator to adjust the focal length of lenses with three levels, and the logic addressing unit contains a decoder to choose a typical lens from a hexagonal lens array. The microfluidic chip control system is connected flexibly to the actuating part, a membrane lens array. It is shown from a simulation and experimental demonstration that the designed and fabricated system, which is composed of a whole microfluidic zoom unit, addressing technology, and a microlens array, works well. Because these components are constructed in the same fabrication process and operate with the same work media and driving source, the system can be made highly compatible and lightweight for applications such as human-machine interfaces and soft robots.

Eccentric magnetic microcapsule for on-demand transportation, release, and evacuation in microfabrication fluidic networks

In this study, an eccentric magnetic microcapsule is reported for on-demand transportation, release, and evacuation in a micro-fabrication fluidic network. Using a droplet-based microfluidic technology, monodisperse magnetic polydimethylsiloxane (PDMS) core-shell microcapsules with a small eccentric ferrofluid core and a big aqueous core are fabricated from templates of dual-cored water-in-oil-in-water ((W1+W2)/O/W) double-emulsion droplets. The eccentric ferrofluid core enables the microcapsule to have flexible magnetic responsibility and orientation property, and the aqueous core can realize the encapsulation and release of nanoliter-scale encapsulants. Each microcapsule can be magnetic-guided by an external magnetic field to travel in microchannels along a designated route, and thus transport the encapsulated cargo (the aqueous core) to the targeted region. Then, the promptly shell rupture of the microcapsule due to the action of a time-averaged electrical stress from Maxwell-Wagner structural polarization in an externally-imposed alternating-current (AC) electric field allows the release of cargo into the region. Moreover, the empty microcapsule after release of the aqueous core can be magnetic-guided to evacuate from the device. Finally, to prove the practical utility of the eccentric magnetic microcapsule for on-demand transportation and release of nanoliter-scale encapsulant, this microcapsule is used to targeted convey fluorescent nanoparticles, which not only can serve as tracers to show the release of the aqueous core, but also can be cargoes themselves for bioimaging, sensing, and so on.

Hierarchical patterning via dynamic sacrificial printing of stimuli-responsive hydrogels

Inspired by stimuli-tailored dynamic processes that spatiotemporally create structural and functional diversity in biology, a new hierarchical patterning strategy is proposed to induce the emergence of complex multidimensional structures via dynamic sacrificial printing of stimuli-responsive hydrogels. Using thermally responsive gelatin (Gel) and pH-responsive chitosan (Chit) as proof-of-concept materials, we demonstrate that the initially printed sacrificial material (Gel/Chit-H+ hydrogel with a single gelatin network) can be converted dynamically into non-sacrificial material (Gel/Chit-H+–Citr hydrogel with gelatin and an electrostatic citrate–chitosan dual network) under stimulus cues (citrate ions). Complex hierarchical structures and functions can be created by controlling either the printing patterns of citrate ink or the diffusion time of citrate ions into the Gel/Chit-H+ hydrogel. Specifically, mechanically anisotropic hydrogel film and cell patterning can be achieved via two-dimensional (2D) patterning; complex external and internal 3D structures can be fabricated in stimuli-responsive hydrogel and other hydrogels that are not stimuli-responsive under experimental conditions (also owing to the erasable properties of Gel/Chit-H+–Citr hydrogel) via 3D patterning; and an interconnected or segregated fluidic network can be constructed from the same initial 3D grid structure via 4D patterning. Our method is very simple, safe and generally reagentless, and the products/structures are often erasable, compatible and digestible, enabling advanced fabrication technologies (e.g. additive manufacturing) to be applied to a sustainable materials platform.

Microfluidic Chamber Design for Controlled Droplet Expansion and Coalescence.

The defined formation and expansion of droplets are essential operations for droplet-based screening assays. The volumetric expansion of droplets causes a dilution of the ingredients. Dilution is required for the generation of concentration graduation which is mandatory for many different assay protocols. Here, we describe the design of a microfluidic operation unit based on a bypassed chamber and its operation modes. The different operation modes enable the defined formation of sub-µL droplets on the one hand and the expansion of low nL to sub-µL droplets by controlled coalescence on the other. In this way the chamber acts as fluidic interface between two fluidic network parts dimensioned for different droplet volumes. Hence, channel confined droplets of about 30–40 nL from the first network part were expanded to cannel confined droplets of about 500 to about 2500 nL in the second network part. Four different operation modes were realized: (a) flow rate independent droplet formation in a self-controlled way caused by the bypassed chamber design, (b) single droplet expansion mode, (c) multiple droplet expansion mode, and (d) multiple droplet coalescence mode. The last mode was used for the automated coalescence of 12 droplets of about 40 nL volume to produce a highly ordered output sequence with individual droplet volumes of about 500 nL volume. The experimental investigation confirmed a high tolerance of the developed chamber against the variation of key parameters of the dispersed-phase like salt content, pH value and fluid viscosity. The presented fluidic chamber provides a solution for the problem of bridging different droplet volumes in a fluidic network.

Fabrication of Complex 3D Fluidic Networks via Modularized Stereolithography

Stereolithography (SL) 3D printer has been used to fabricate microfluidic channels albeit with limitations in the attainable channel geometries. In the article, number 1901109 by Michinao Hashimoto and co-workers, the fabrication of complex networks of microchannels is enabled by the modularization of fluidic networks into printable elements. 2D and 3D networks of microchannels with high complexity (i.e., multiple branching networks) and small cross-sectional dimensions (w = 75 μm and h = 100 μm) are readily achieved.