Butea Monosperma Flowers Research Articles

Green Synthesis and Characterization of Cobalt Nanoparticles Using Butea Monosperma Flower Extract and their Biocompatibility Studies

Green Synthesis and Characterization of Cobalt Nanoparticles Using Butea Monosperma Flower Extract and their Biocompatibility Studies

Dyeing of pashmina wool using Butea monosperma flower extract

Dyeing of pashmina wool using Butea monosperma flower extract

An Assessment of the In Vitro Antioxidant Activity of Cobalt Nanoparticles Synthesized From Millettia pinnata, Butea monosperma, and Madhuca indica Extracts: A Comparative Study.

Objective This study aimed to synthesize cobalt nanoparticles (CoNPs) via the green synthesis method using Millettia pinnata(M. pinnata), leaf (MPL), Butea monosperma(B. monosperma)flower (BMF), and Madhuca indica(M. indica)flower (MIF) as eco-friendly reducing agents. It further aimed to compare the effectiveness of these plant extracts in CoNPs production and evaluate the antioxidant activities of the synthesized nanoparticles (NPs), establishing a link between the phytochemical constituents of the extracts and the antioxidant capacity of CoNPs for potential applications in drug development and environmental sustainability. Materials and methods CoNPs were synthesized using aqueous extracts of MPL, BMF, and MIF. These extracts act as stabilizing and self-reducing agents. Initially, the presence of CoNPs was detected visually by observing a color change. To confirm this observation, UV-visible spectroscopy and Fourier transform infrared (FTIR) spectroscopy were employed. UV-visible spectroscopy helps in analyzing the absorption of light by the CoNPs, while FTIR spectroscopy is used to identify the functional groups present in the NPs. Subsequently, the antioxidant activity of the synthesized CoNPs was assessed using the 1,1-diphenyl-2-picryl hydroxyl (DPPH) radical-scavenging assay. This assay measures the ability of antioxidants to neutralize free radicals by determining the reduction in the DPPH radical's absorption. To ensure the reliability of the results, the experiments were conducted in triplicate. Statistical analysis was then performedto compare the antioxidant effectiveness of the different plant extracts used in synthesizing the CoNPs. This analysis helps in determining any significant differences in antioxidant activity among the extracts. Results UV-visible spectral analysis confirmed the successful synthesis of CoNPs, revealing characteristic absorption peaks. For M. pinnata leaf extract (MPLE), the maximum peak was observed at ~272 nm, while B. monosperma flower extract (BMFE) exhibited a peak at ~276 nm, and M. indica flower extract (MIFE) revealed a maximum peak at ~320 nm. FTIR analysis further validated the presence of organic molecules from plant components on the outer layer of CoNPs, indicating successful capping and stabilization by phytochemicals from the extracts. The spectra displayed various peaks at different wavenumbers: MPLE showed prominent peaks at 3335 cm-1, BMFE showed distinct peaks at 3314 cm-1, and MIFE exhibited significant peaks at 3261 cm-1. Among the three types of CoNPs tested, those synthesized using MIFEexhibited the highest inhibition of 87.67% at a concentration of 60 µL. This higher inhibition was compared to those synthesized using BMFEand MPLE. This study suggests that the CoNPs synthesized on MIFEcan serve as an antioxidant agent because of their remarkable free radical-scavenging activity. Conclusions The study highlights the potential of CoNPs synthesized using MIFE as they exhibited superior antioxidant activity compared to those synthesized with BMFE and MPLE. Therefore, the study underscoresthe promise of MIFE as a valuable natural resource for producing CoNPs abundant in antioxidants. Furthermore, it emphasizes the importance of implementing environmentallyfriendly synthesis techniques to produce nanomaterials that are both safe for the environment and biologically effective.

Formulation and Development of Compact Powdered Blush by Using Natural Colour from Flowers of Butea Monosperma

Formulation and Development of Compact Powdered Blush by Using Natural Colour from Flowers of Butea Monosperma

Marker Based Standardization of Herbal Sunscreen Formulation by Using Validated High-Performance Thin Layer Chromatography Method

Plant based products are worldwide used and trusted for the care and cure of the health. Herbal sunscreen formulations are gaining popularity for their effectiveness and are devoid of side effects. The present investigation is an effort made to develop herbal sunscreen cream containing methanolic extracts of different plants and its standardization for the presence of bioactive compounds. The leaves of Cymbopogon citratus (Stapf), fruit peel of Punica granatum (Linn), flowers of Butea monosperma (Lam.) and leaves of Neolamarckia cadamba (Roxb.) were selected for the preparation of sunscreen cream. The standardization of herbal formulations is very important to determine its quality based on the concentration of their active phytoconstituents. The herbal cream F-5 (2 % w/w) imparted its sun-protective and antioxidant property. It shows free radicals scavenging activity due the presence of flavonoids and phenolic compounds. High performance thin layer chromatography method is used to determine the quality and quantity of the sun protective phytoconstituents present in the product. The method was validated according to ICH guidelines for the estimation butrin (BT), isobutrin (IBT), quercetin (QC), apigenin (API), chlorogenic acid (CA) and gallic acid (GA) using the optimized solvent systems. The estimation of bioactive markers was carried out on silica gel precoated thin layer chromatography plates with 60F254 as the stationary phase and Camag TC scanner III for densitometric scanning. The average Rf values for the markers were found to be 0.46 for BT, 0.57 for IBT, 0.50 for QC, 0.57 for API, 0.66 for CA and 0.42 for GA. The developed HPTLC method was linear with correlation coefficient 0.999 for BT, 0.998 for IBT, QC, API and 0.9966 for CA and 0.9989 for GA. Limit of detection (LOD) and limit of quantification (LOQ) were recorded. The developed analytical method for quantitative determination of phytoconstituents was found efficient, simple, accurate, and validated.

Synthesis, characterization, and in vitro DPPH radical scavenging assay study of PEG-capped carbon nanoparticles derived from Butea monosperma flower extract

In this work, the core carbon nanoparticles (BM CNs) and polyethylene glycol 400 (PEG400)-capped BM CNs were synthesised from Butea monosperma flower extract via the facile direct heating method. They were investigated for their intriguing properties and in vitro antioxidation activity in comparison with Carica papaya seed (CPS) extract via DPPH assay. The CNs were characterised by DLS, XRD, HR-TEM, TGA, FT-IR, UV–vis spectral analysis and quantum yield measurement. The PEGylated CNs demonstrated decent quantum yield, favourable size, and turbostratic carbon phase apart from better stability and dispersion nature as compared to the uncapped entity. The mildly stable uncapped sample with zeta potential −17.9 mV featured its extensively aggregated form, unlike the capped sample with −23.0 mV zeta potential, which sheds light on its enhanced stability by PEG400. Antiradical capacities of the CNs in comparison with CPS extract demonstrated the PEG400-bound nanomaterial of its superior antiradical activity against the other two controls. This study provides an insight into the novel polymer-CNs’ potent antioxidant property which can be further explored for its incorporation in targeted drug delivery and other biomedical applications.

Butea Monosperma Shows Anti-cancer and Anti-Oxidant Activity: In-vitro and In vivo Study

Breast cancer is one of the predominant cancers in Indian subcontinent and the global scenario is not very different. Treatment of this type of cancer is possible by chemotherapy, surgery, and radiotherapy, which relieve the patient; however, off target activity and severe toxicity of non-cancerous cells remained the major drawbacks of these chemotherapeutics. In order to overcome these issues, modern cancer therapeutics emphasizes the implication of natural bioactive compounds. In this current piece of work, we have studied the anti-cancer potentiality of Butea monosperma flower in in vitro breast cancer cells. Various extracts of this flower were found to be non-toxic to breast epithelial cells but toxic to breast cancer cells. However, the methanol extract of this flower was most effective amongst all other extracts. Data suggest that Butea monosperma flower induce DNA damage mediated apoptosis and inhibit cell proliferation, angiogenesis and metastasis. It also inhibits the angiogenesis and metastasis in xenograft mice model system. Moreover, the anti-cancer phenomenon was found to be induction of inflammatory cytokines mediated oxidative stress in breast cancer. Collectively, Butea monosperma flower shows excellent cancer therapeutics in order to induce DNA damage, inhibit angiogenesis and metastasis simultaneously.

Antibacterial efficiency of naturally occurring dyes and mordants

The popularity of natural dyes is steadily increasing due to their properties such as soothing colors, non-carcinogenic & non-hazardous nature, and also being safe for the environment. Some natural dyes also have been reported to have antibacterial properties. This study was performed to explore the antibacterial efficiency of selected plant products that were used in the dyeing of fabric. Flowers of Butea monosperma and Tagetes erecta were used for the dye extraction. Banana pseudostem sap and Punica granatum rinds taken as biomordant. High-performance thin-layer chromatography was conducted for the identification of components of B. monosperma, T. erecta, Banana (Musa sp.) pseudostem sap and P. granatum rind. Dyeing of two types of cotton fabrics was performed at pre-optimized conditions. The antibacterial activities of dyed fabrics were evaluated qualitatively and quantitatively. Various phytochemical constituents in the dyeing plant materials already have antibacterial properties. Fabrics dyed with B. monosperma, T. erecta, Banana (Musa sp.) pseudostem sap and P. granatum rind showed high resistance towards Escherichia coli and Staphylococcus aureus.

EXTRACTION OF NATURAL DYE FROM BUTEA MONOSPERMA (LAM.) TAUB. FLOWER DYEING COTTON - POLYESTER FABRIC

Natural dyes are emerging globally as eco-friendly synthetic colorant. Natural dyeing is process of applying coloring matter directly on fiber, yarn or fabric without any additives. Butea monosperma (Lam.)Taub. is one of most important ornamental plants mainly grow in forest and rich in orange pigments. In the present study, the dyeing pigments present in naturally drop flowers of Butea monosperma (Lam.) Taub. Extraction by using four different Mordant with aqueous extraction method and one Ethno botanical local method on cotton-polyester mix fabric. The result revealed that, different color like light yellow, orange, brown, cream etc. were obtained from the dye when subjected to Mordant. The crud flower petals dye after further purification and refining has tremendous scope as medicine and also serve a coloring material in soft drink and other food product like jam, chow main, noodles, sausage etc. Natural dye from the flower of Butea monosperma (Lam.) Taub. In the field of textile, food item and pharmaceutical industry.

Phytochemical screening of Butea monosperma (Lam.) Taub. Flowers and chlorophyll content in leaves of South-eastern Rajasthan, India

The present study was undertaken to determine the presence of phytochemicals in the flowers and chlorophyll content in the leaves of Butea monosperma in South-eastern Rajasthan, India. Butea monosperma is a large deciduous tree popularly known as Flame of the Forest, belongs to family Fabaceae. Almost all the parts of the plant namely root, leaves, fruit, stem bark, flowers, gum are used as medicine, food, fibre and for other miscellaneous purposes such as fish poison, dye, fodder, utensils, etc. Samples of flowers and leaves were collected from 4 different locations (Kota, Bundi, Baran and Jhalawar district) from south-eastern Rajasthan, and 12 different individuals of Butea monosperma trees (3 individuals from each site). Methanol and ethanol were used to extract and screening of phytochemicals present in flowers and leaves. The present results revealed that flowers of Butea monosperma indicate the presence of phenol, alkaloid, flavonoid, anthocyanin, xanthophyll, saponins, β-carotene, lycopene, total carotenoid in the methanol extract whereas the flowers subjected to extract with ethanol indicate the presence of phenol, alkaloid, flavonoid, xanthophyll, saponins, β-carotene, lycopene and total carotenoid. Chlorophyll content analysis showed that leaf of Butea monosperma had chlorophyll-a (1.91±0.02), chlorophyll-b (1.26±0.03) and total chlorophyll (3.18±0.03). The presence of phytochemical in the flower might find application for medicinal purpose and presence of chlorophyll pigment suggest the photosynthetic efficacy of plant.

English

English

Production of value added Oyster mushrooms

Mushrooms are ironic in nutrimental resources and have converted into one of the common foods in the previous twenty years globally. The types of edible mushrooms are button, milky and oyster mushrooms. The research aimed at value addition of Oyster mushrooms by rising on paddy substrate complemented with two concentrations of four different medicinal plants. The plant parts selected were flowers of Butea monosperma, leaves of Moringa olifera, bark of Cinnamonom zeylianicum, fruits of Corindraum sativum at 5% and 10% concentration. Different species of oyster mushrooms are available. In the present study Pleurotus florida was selected for value addition. Maximum mycelium running rate was detected in Cinnamon bark (5%) and paddy straw (95%) accompanied group and lowermost running rate of mycelium was observed in Moringa leaf (5%) and paddy straw (95%). The primordial arrival was fast with Cinnamon bark (5%) supplemented group and slowest in Moringa leaf (5%) supplemented group. The mushrooms grownup on Coriander fruit (5%) produced more but the growth was slow. Mushrooms supplemented with Butea flower (5%) exhibited slow growth and yield was next to coriander fruit supplemented mushrooms. The produced mushrooms were subjected to physical evaluation, preliminary phytochemical testing and also tested for estimation of total flavonoids, total phenols, total tannins and total cinnamaldehyde contents. Value addition of Oyster mushrooms was successful with cinnamon bark as cinnamaldehyde was noticed in the Cinnamon bark supplemented group, and also with Moringa leaves as flavonoids was observed in more concentration in Moringa leaf supplemented mushroom group.

Functional characterization and in vitro screening of Fructobacillus fructosus MCC 3996 isolated from Butea monosperma flower for probiotic potential.

The fructophilic bacterium Fructobacillus fructosus MCC 3996 described in the present investigation was isolated from the nectar of Butea monosperma flower and evaluated in vitro for the manifestation of probiotic features. The strain utilizes fructose faster than glucose and is capable to grow in the range of 1-35% fructose concentration (optimum 5% w/v) and thus denotes its fructophilic nature. In vitro assessments of the strain have examined for the endurance in acidic environment/gastric juice, the better auto-aggregation ability even in the presence of hydrolytic enzymes, co-aggregation with pathogenic bacteria, hydrophobicity properties and no haemolytic activity to elucidate its feasible probiotic use. The significant antagonistic activity against several detrimental bacteria, despite lacking the bacteriocin secretion, is an astonishing feature. Owing to the indigenous origin of the isolate, it could be used as a probiotic, starter culture, and/or the active ingredient of food formulation may contribute to improve the desirable fermentation, long-term storage and nutritional benefits of foods especially rich in fructose. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provided in vitro evidence that Fructobacillus fructosus MCC 3996 have endurance in acidic gastric juice, better co-aggregation, auto-aggregation properties, splendid antagonistic activities against several bacteria involved in food spoilage/human infections, pertinent antibiotic susceptibility profile and no haemolytic activity. Also, F. fructosus have the capability to survive in the appreciable amount of fructose, and this advocates that the strain could be used as starter culture and/or the active ingredient of fructose-rich foods. The current in vitro study provided a strong basis for further in vivo research to identify the health beneficial characteristics of F. fructosus and its potential could be effectively utilized as health-boosting ingredient in food and pharmaceutical industries.

Butea monosperma flower extract partially reduces high-fat diet induced obesity in experimental rats

BackgroundThe body fat reduction has been a major health goal of obese individuals throughout the world and identifying functional foods that can assist in alleviatingthe excess body fat has been the objective of various studies. ObjectiveThe current study was investigated to evaluate the anti-obesity profile of Butea monosperma (BM) flower extract on the high-fat diet (HFD) induced obese rats. Material & methodsCD and AD diet was fed for 40 days to induce obesity in Female albino rats. Three different doses of BM flower extract (100, 200 and 400 mg/kg, per oral) were administered every day to the drug treatment groups. The observations were made for body weight measured at every 5 day interval, various organs and body fat pad weights, body temperature, locomotor activities along with total cholesterol, triglyceridesand glucose levels were analyzed on the 39th and 40th day. ResultBM flower extract 400 mg/kg (high dose) treated rats showed a significant decrease in body weight, total cholesterol, triglycerides levels, and glucose levels with marked elevation in locomotor activities and body temperatures. ConclusionThe findings suggests that the supplemented BM flower extract in the CD and AD have normalized the weight gain parameters and can be a future herbal candidate for reversing obese individual to normal.

Ammonia ligand and transition metal salt complexation on naturally dyed textile surface: A reflectance spectroscopic investigation to understand coloration and fastness properties

Ammonia is a stronger ligand and can replace water molecules in a chelate metal complex as per spectrochemical series. This property of ammonia was utilized in this work for the coloration of wool in order to obtain some unusual effects. Different concentrations of ammonia solution was applied on Butea monosperma flower extract dyed wool previously premordanted with ferrous sulfate salt to evaluate the effect on colorimetric properties. A range of newer shades were produced on wool substrates which were characterized in terms of K/S, CIELa*b* values and fastness properties. The observed effects exhibited on wool and acceptable fastness properties of produced shades points out a promising approach for developing environmentally sustainable colorful textile materials and clothing. The chemical modification of wool before and after application of ammonia was also evaluated using Fourier transform infrared spectrometer analysis. © 2019 American Institute of Chemical Engineers Environ Prog, 38:e13146, 2019

WOUND HEALING POTENTIAL OF METHANOLIC EXTRACT OF FLOWERS OF BUTEA MONOSPERMA LINN. IN DIABETIC ANIMALS

The main of our study is to evaluate the Wound Healing Potential of Methanolic Extract of Flowers of Butea monosperma Linn. in Diabetic Animals. Methanolic extract was prepared by continuous hot extraction method by soxhlet apparatus. Preliminary phytochemical screening showed the presence of flavonoids, phenolic compounds and some glycosides. Diabetes was induced by single injection of Alloxan monohydrate in Wistar albino rats and their blood glucose levels were measured. Excision wound model was used for creation of wound in diabetic animals and methanolic extract was administered in diabetic animals to observe its effect. Methanolic extract significantly lowered the blood glucose level and highly significantly showed wound contraction in diabetic animals. The preliminary phytochemical analysis of the Butea monosperma flower extract showed the presence of tannins, flavonoids and triterpenoids. As per previous literature survey, flavonoids are responsible for anti-diabetic and wound healing activity. So this wound healing effect of Butea monosperma may be due to presence of flavonoids in plant. Hence present research supports traditional claims of the plant in wound healing.
 Keywords: Butea monosperma, Diabetic animals, Alloxan monohydrate, Methanolic Extract, Wound Area

Cotton Khadi fabric dyeing with natural dye extracted from the petals of Butea monosperma Flower using different mordants

Cotton Khadi fabric dyeing with natural dye extracted from the petals of Butea monosperma Flower using different mordants

A comparative study of the in vitro enzyme inhibitory and antioxidant activities of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret from Pakistan: New sources of natural products for public health problems

Infusions, decoctions and tinctures were prepared from flowers of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret and evaluated for in vitro inhibition of enzymes implicated on the onset of neurological diseases (acetylcholinesterase: AChE and butyrylcholinesterase: BuChE), diabetes (α-glucosidase and α-amylase), obesity (lipase) and skin hyperpigmentation (tyrosinase). Extracts were also appraised for radical scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and for metal chelating activity on copper and iron ions. Samples were evaluated for their total contents in different phenolics groups by spectrophotometric methods, for phenolic profile by high performance liquid chromatography e diode array detection (HPLC-DAD) and for mineral contents by microwave plasma-atomic emission spectrometry (MP-AE). Regarding B. monosperma, the tincture allowed for a moderate inhibition of AChE, the decoction was able to inhibit α-glucosidase and no activity was observed towards BuChE, α-amylase or lipase. All extracts had a low or moderate inhibition towards tyrosinase, and significant RSA and metal chelating potential. As for S. grandiflora, only the decoction inhibited AChE, none of the extracts was able to inhibit BuChE, all samples inhibited α-glucosidase and infusions and decoctions had similar inhibitory properties towards α-amylase. None of the extracts was active against lipase, but all were able to inhibit tyrosinase. Extracts had also significant RSA, moderate copper chelation and decoctions had the capacity to chelate iron. The most abundant macroelements in both species were potassium and calcium, while iron was the prevalent microelement, especially in B. monosperma. Both species had significant levels of phenolic compounds, and the main components in decoctions and infusions of B. monosperma were syringic and salicylic acids, while the major compound identified in tinctures was the flavonoid luteolin-7-O-glucoside. In S. grandiflora the most abundant were chlorogenic and neochlorogenic acids and catechin hydrate. Molecular docking studies on the most abundant molecules in S. grandiflora, (+)-catechin, chlorogenic acid and neochlorogenic acid, indicate that these compounds are able to dock to α-glucosidase in a similar manner than acarbose. Our results suggest that flowers of both species are a promising source of high value-added compounds with enzyme inhibitory and antioxidant properties.

Breast cancer amelioration by Butea monosperma in-vitro and in-vivo

Ethnopharmacological relevanceButea monosperma belonging to family Fabaceae is used in the Indian traditional medicine (Ayurveda) for various ailments including abdominal tumors and possess anti-estrogenic activity. Aim of the studyThe present study is aimed at investigating the chemo-preventive potential of Butea monosperma in breast cancer and elucidating it's mechanism of action by assessing its effect on key processes like apoptosis, angiogenesis and metastasis. MethodsCytotoxic potential of methanol extract of Butea monosperma flower (MEBM) was tested in MCF-7 (estrogen receptor positive), MDA-MB-231 (triple negative) and MDA-MB-453 (HER2 positive) human breast cancer cells by MTT assay. Chemo-preventive potential was evaluated in-vivo in Methylnitrosourea (MNU) induced mammary cancer in nulliparous Sprague-Dawley rats. The mechanism for anticancer potential was screened by in-vitro studies involving Annexin V- FITC assay (apoptosis), Chick Chorioallantoic Membrane assay (angiogenesis) and Migration assay (metastasis). Statistical analysis was done by one way and two way ANOVA (for Growth Rate and feed consumption efficiency) followed by post hoc Bonferroni's test with P value < 0.05. ResultsIt is observed that the exposure of MEBM, at various concentrations and time intervals to different cell lines, resulted in decreased cell proliferation. The IC50 value of MCF-7 cells was found significantly less than that of MDA-MB-231 and MDA-MB-453 cells, which indicated that the extract of said medicinal plant were more potent inhibitors of estrogen positive breast cancer cells than other types of breast cancer cells in vitro. Corroborative evidences were acquired in MNU actuated mammary carcinogenesis where MEBM constricted tumor parameters, decreased expression of estrogen and progesterone, nucleic acid content and increased latency period. MEBM also induced apoptosis, inhibited angiogenesis and metastasis in-vitro. ConclusionSelective cytotoxic activity in MCF-7 estrogen positive breast cancer cells and inhibition of growth of mammary carcinoma in-vivo by methanol extract of Butea monosperma flowers (MEBM) suggests chemo-prevention through modulation of estrogen and progesterone receptor, apoptotic, anti-angiogenesis and anti-metastatic activity.

Exploiting the potential of polyphenolic biomordants in environmentally friendly coloration of wool with natural dye from Butea monosperma flower extract

ABSTRACTColoration of textiles with natural dyes involves huge consumption of chemicals; mainly, metal salt mordants, which pose serious environmental challenges. To mitigate this pollution, more and more environmentally friendly, biodegradable, biocompatible and renewable products should be popularized in dyeing industry. This paper proposes a sustainable and metal salt-free dyeing of wool samples by employing natural mordants extracted from pomegranate peel (Punica granatum L), gallnut (Quercus infectoria L) and catechu (Acacia catechu). The color parameters of dyed wool samples were greatly found to be dependent on the chemical compounds present in respective biomordants and their interaction ability with the functional groups of wool and the dye molecules. The investigated biomordants exhibit different interactions with coloring compounds of Butea monosperma (palas) dye resulting in deep brown, olive green, dark brown, cinnamon, burgundy, and yellowish hues on wool. All the biomordants selected in this study improved dye performance and resulted in a broad beautiful spectrum of colors with acceptable fastness properties. The results encourage the search and exploitation of new plant species as a source of biomordants to replace metallic and toxic mordants currently used in textile industry.