Reduction In Flow Research Articles

Comprehensive assessment of waste heat recovery mismatch and renewable energy integration in data centers: A multifaceted energy, economic, and environmental perspectives

The integrated utilization of free cooling sources, waste heat recovery from information technology equipment, and renewable energy emerges as a pivotal method for conserving energy and mitigating carbon emissions in data centers. However, there is limited research on systems that integrate these three technologies. A notable mismatch arises between the waste heat generated and the requirements for building heating, but it is often overlooked. Moreover, a discernible lack of comprehensive analysis concerning the multifaceted influences associated with using renewable energy exists. Therefore, this paper employs a range of metrics to comprehensively assess a renewable energy integration system considering the mismatch of the waste heat recovery in data centers. The results indicate that due to mismatch characteristics, the energy-saving rate of heating systems will not constantly climb with the increase in energy savings. It causes only 45.5% of waste heat available for heating purposes achieving a power usage effectiveness of 1.17 and the rest will be abandoned. Similarly, its economic performance doesn't absolutely improve with the expansion in heating volume, the payback period doesn't continue to fall but increases after reaching the optimal level of 5.7. The proposed system accomplishes a 45.8% reduction in energy consumption for the cooling system, a notable decrease of 90% in carbon emissions, and a 55.7% reduction in annual cash flow. It is also important to note that while integrating battery systems aids in stabilizing grid disturbances, with a minimum disturbance index of 0.2, it introduces significant economic costs for the data centers.

A combined active control method of restricted nonlinear model and machine learning technology for drag reduction in turbulent channel flow

A combined active control method of restricted nonlinear model and machine learning technology for drag reduction in turbulent channel flow

Ischemic core volumes and collateral status have diurnal fluctuations – A retrospective cohort study of 18,137 patients

IntroductionRecent observations suggest that circadian rhythms are implicated in the timing of stroke onset and the speed of infarct progression. We aimed to replicate these observations in a large, multi-center, automated imaging database. MethodsThe RAPID Insights database was queried from 02/01/2016 to 01/31/2022 for patients with perfusion imaging and automated detection of an ischemic stroke due to a presumed large vessel occlusion. Exclusion criteria included: patient age ≤25, mismatch volume of <0 cc, and failure to register a positive value on either relative cerebral blood flow (rCBF) reduction of 38% less than normal or total mismatch volume. Imaging time was subdivided into three epochs: Night: 23:00h-06:59h and Day: 07:00h-14:59h, and Evening: 15:00h-22:59h. Perfusion parameters were defined using standard conventions for core volume, penumbra, and collateral circulation (measured via the Hypoperfusion Intensity Ratio, HIR). Statistical significance was tested using a sinusoidal regression analysis. ResultsA total of 18,137 cases were analyzed. The peak incidence of stroke imaging of patients with LVOs occurred around noon. A sinusoidal pattern was present, with larger ischemic core volumes and higher HIR during the night compared to the day: peak ischemic core volume of 23.4 cc occurred with imaging performed at 3:56 AM (p<0.001) and peak HIR of 0.35 at 3:40 AM (p<0.001). ConclusionWe found that ischemic core volumes were larger and collateral status worse at nighttime compared to daytime in this large national database. These findings support prior data suggesting that poor collateral recruitment with subsequent larger ischemic stroke volumes may occur at night.

Real-time regulation of detention ponds via feedback control: Balancing flood mitigation and water quality

Detention ponds can mitigate flooding and improve water quality by allowing the settlement of pollutants. Typically, they are operated with fully open orifices and weirs (i.e., passive control). Active controls can improve the performance of these systems: orifices can be retrofitted with controlled valves, and spillways can have controllable gates. The real-time optimal operation of its hydraulic devices can be achieved with techniques such as Model Predictive Control (MPC). A distributed quasi-2D hydrologic-hydrodynamic coupled with a reservoir flood routing model is developed and integrated with an MPC algorithm to estimate the operation of valves and movable gates in real-time. The control optimization problem is adapted to switch from a flood-related algorithm focusing on mitigating floods to a heuristic objective function that aims to increase the detention time when no inflow hydrographs are predicted. The case studies show the potential results of applying the methods developed in a catchment in Sao Paulo, Brazil. The performance of MPC compared to alternatives that do not change the operation over time with either fully or partially open valves and gates are tested. Comparisons with HEC-RAS 2D indicate volume and peak flow errors of approximately 1.4% and 0.91% for the watershed module. Simulating two consecutive 10-year storms shows that the MPC strategy can achieve peak flow reductions of 79%. In contrast, the passive scenario has nearly half of the performance (41%). A 1-year continuous simulation results show that the passive scenario with 25% of the valves opened can treat 12% more runoff compared to the developed MPC approach, with an average detention time of approximately 6 h. For the MPC approach, the average detention time is nearly 14 h, indicating that both control techniques can treat similar volumes; however, the proxy water quality for the MPC approach is enhanced due to the longer detention times achieved.

Effects of algal organic matters on microporous ceramic emitters clogging in agricultural water distribution systems: Experiment and molecular simulation investigations

The mechanism by which algal organic matter (AOM) affects the clogging of ceramic emitters remains unclear, which partially reduces the operational life of agricultural water distribution systems. This paper systematically investigated the clogging phenomenon of ceramic emitters under three different AOM concentrations. The results of irrigation tests revealed that the AOM significantly affects the degree of clogging of ceramic emitters, with higher AOM concentrations leading to faster flow reduction. By analyzing the original irrigation water and effluent and characterizing the clogged emitter surface, it was demonstrated that AOM was intercepted by the ceramic emitter, forming a dense biofilm. Infrared spectroscopy analysis revealed that polysaccharides and humic substances were the main clogging components. The clogging kinetics showed that as the AOM concentration increased, the clogging of the filter cake layer gradually become dominant. Further, the mechanism of interaction between AOM and silica ceramic emitters was explored from a microscopic perspective using molecular dynamics (MD) simulation with bovine serum albumin (BSA), sodium alginate (SA), and humic acid (HA) as model clogging substances in AOM. The simulation results indicated a strong interaction between AOM molecules and silica molecules dominated by electrostatic attraction, with the strength of the interaction as SA > HA > BSA. It was hypothesized that early clogging was mainly formed by polysaccharides and humic substances combining with silica molecules, while BSA was retained later by combining with organics on the clogging layer or through size exclusion. This study provides insights into bio-clogging in microporous ceramic emitters and may offer a theoretical basis for developing measures to control emitter clogging.

What leads some countries to experience larger decreases in foreign flows during low-flow episodes? Evidence from international portfolio flows

This paper investigates the drivers explaining the heterogeneous responses of foreign portfolio investors across 43 emerging markets and developing economies (EMDEs) during low-flow episodes. Our investigations reveal several findings: (a) During low-flow episodes, EMDEs with stronger macroeconomic/institutional fundamentals—e.g., larger foreign reserves, less public indebtedness, and better institutional quality—suffer fewer reductions in foreign inflows. (b) EMDEs with more open/developed financial markets attract more portfolio inflows during surges but suffer larger declines during stops. Hence, we provide evidence supporting the foreign investor differentiation (according to fundamentals) and the mixed blessing of financial sectors hypothesis.

Hydraulic models of silled orifices and stilling basins in online dams for flood reduction

ABSTRACT Building flood reduction dams for territory protection is very often necessary as it allows the reduction of peak flows during flood events by creating a storage in upstream areas. An online structure with such characteristics is outlined, presenting one or more openings in order to allow normal and moderate flows to pass downstream. A stilling basin is provided downstream of the openings and, on one side of the flooded area, a spillway weir is set. For low river flows, the openings will act as free-surface weirs. The openings are equipped with a sill in such a way to let the discharge independent of the downstream flow conditions. For moderate flood flows, the water level upstream of the dam will rise, and the openings will become submerged, effectively behaving as an orifice arrangement. For large flood flows, the crest of the flood spillway weir is reached and they will be discharged via both the orifice structure and the flood spillway structure. The silled openings and the stilling basin are one only hydraulic complex. Since no examples exist of their behavior as a whole, the aim of this paper is to give the designers the opportunity to size them given the basic requirement of the flood reduction dam, i.e. the max discharge allowed downstream. For this reason, the paper will focus on the opening discharge coefficients, the stilling basin energy dissipation and water level. Both the orifices and the stilling basins were studied starting from the hydraulic models of three dams that were built in Calabria (Italy). The structures of the stilling basins are original. The results appear in dimensionless form and offer useful guidance for similar hydraulic cases, with the exception of specific terrain related issues. Finally, an example of a preliminary design of orifices and stilling basin is presented.

The curse of COVID-19 on foreign direct investment: assessing the role of property rights amidst the pandemic

ABSTRACT How did the COVID-19 pandemic impact foreign direct investment (FDI)? Following the outbreak of COVID-19, the overall volume of FDI inflows plummeted. However, COVID-19’s adverse effects on FDI in host countries exhibited discernible variability contingent upon the characteristics of domestic institutions, particularly the robustness of property rights within these countries. While host countries with strong property rights experienced a mitigated negative impact on FDI, contrary to general expectations, our analysis suggests that host countries with weak property rights may have also evaded a significant reduction in FDI inflows during the pandemic. This was primarily because both strong and weak property rights institutions in host countries conveyed positive signals to investors, which may have incentivized them to continue their investments even amidst the pandemic-induced crisis. Analyzing a time-series cross-sectional dataset of 126 developing countries covering the period 2009–2021, our findings reveal a U-shaped relationship between FDI and property rights. This implies that COVID-19’s impact manifested as a weak negative force on FDI inflows in host countries with weak property rights as well as those with strong property rights. Conversely, host countries with moderately strong property rights tended to suffer a substantial reduction in FDI.

Persistent Gene Flow Suggests an Absence of Reproductive Isolation in an African Antelope Speciation Model.

African antelope diversity is a globally unique vestige of a much richer world-wide Pleistocene megafauna. Despite this, the evolutionary processes leading to the prolific radiation of African antelopes are not well understood. Here, we sequenced 145 whole genomes from both subspecies of the waterbuck (Kobus ellipsiprymnus), an African antelope believed to be in the process of speciation. We investigated genetic structure and population divergence and found evidence of a mid-Pleistocene separation on either side of the eastern Great Rift Valley, consistent with vicariance caused by a rain shadow along the so-called 'Kingdon's Line'. However, we also found pervasive evidence of both recent and widespread historical gene flow across the Rift Valley barrier. By inferring the genome-wide landscape of variation among subspecies, we found 14 genomic regions of elevated differentiation, including a locus that may be related to each subspecies' distinctive coat pigmentation pattern. We investigated these regions as candidate speciation islands. However, we observed no significant reduction in gene flow in these regions, nor any indications of selection against hybrids. Altogether, these results suggest a pattern whereby climatically driven vicariance is the most important process driving the African antelope radiation, and suggest that reproductive isolation may not set in until very late in the divergence process. This has a significant impact on taxonomic inference, as many taxa will be in a gray area of ambiguous systematic status, possibly explaining why it has been hard to achieve consensus regarding the species status of many African antelopes. Our analyses demonstrate how population genetics based on low-depth whole genome sequencing can provide new insights that can help resolve how far lineages have gone along the path to speciation.

The Effect of the Levonorgestrel-Releasing Intrauterine System on Myometrial Blood Flow in Patients With Heavy Menstrual Bleeding.

Background Abnormal uterine bleeding constitutes a vexing issue among female patients, substantially impacting their quality of life. Surgical interventions, particularly hysterectomy, contribute to the psychological, physical, and financial burden on families and, by extension, the healthcare system. Levonorgestrel-releasing intrauterine system (LNG-IUS) represents a conservative management approach and emerges as a beneficial option for affected patients. The present study aims to elucidate color Doppler changes in the uterine artery pre- and post-LNG-IUS insertion. Objective The primary objectives encompass an investigation into the variations in Doppler indices (resistance index (RI) and pulsatility index (PI)) within the arcuate and radial branches of the uterine artery, as well as the assessment of endometrial thickness before LNG-IUD insertion, at three months, and six months post-insertion. Secondary outcomes include evaluating changes in pictorial blood assessment chart (PBAC) scores before insertion, at three months, and at six months after LNG-IUS insertion. Methods A cross-sectional study was conducted at the Department of Obstetrics and Gynecology at All India Institute Of Medical Sciences (AIIMS), Nagpur. A cohort of 25 women underwent LNG-IUS insertion. The endometrial cavity, RI, and PI of both arcuate and radial arteries were assessed before LNG-IUS insertion and at three and six months after insertion. Results The PI of the arcuate artery exhibited minimal alteration over the six-month duration, with a p-value of 0.43. Conversely, the RI demonstrated a statistically significant increase over the same period (p = 0.03). Conversely, the radial artery exhibited no statistically significant changes in either PI or RI (p = 0.39 or 0.13, respectively). Conclusion Following six months of LNG-IUS utilization, a substantial reduction in endometrial thickness and menstrual flow was observed, concomitant with an improvement in hemoglobin levels. Notably, the PI of both the arcuate and radial arteries demonstrated no significant change. Although the RI of the arcuate artery increased, its clinical relevance may be limited. Consequently, the observed reduction in menstrual bleeding cannot conclusively be ascribed to diminished blood flow in uterine arteries.

LID Implementation in an urban basin: a Brazilian case study

Problems related to urban drainage systems and the disorderly growth of large urban centers have led to the search for alternative drainage techniques. These techniques have been called Low Impact Development (LID), and generally influence the reduction of peak flow and runoff volume. In an urban sub-basin in the city of Recife, PE, the hypothesis of replacing existing roofs with a green roof (GR) was considered in three scenarios: (1) pre-urbanized, (2) current, (3.1) 30% of GR, (3.2) 50% of GR, (3.3) 75% of GR, (3.4) 100% of T.V. For this, simulations were carried out in PCSWMM based on the current urbanization situation. Linimetric readings were taken for calibration, obtaining 0.61 NSE and 0.903 R². Validation was carried out using images at two points within the basin. The reduction in peak flow values ranged from 0.74 to 2.10 m³/s, in addition to the time being delayed from 31 to 90 minutes. As for river level values, the variation was between 4 and 13 cm, while volume reductions recorded values between 67.42 and 190.81 m³. Overall, this proposed methodology can help stormwater managers better evaluate the performance of LID techniques at different hydrological scales, showing the importance of prioritizing urban adaptation and green infrastructure implementation.

Giant unruptured middle cerebral artery aneurysm revealed by intracranial hypertension: is a systematic decompressive hemicraniotomy mandatory?

Our study aimed to evaluate the postoperative outcome of patients with unruptured giant middle cerebral artery (MCA) aneurysm revealed by intracranial hypertension associated to midline brain shift. From 2012 to 2022, among the 954 patients treated by a microsurgical procedure for an intracranial aneurysm, our study included 9 consecutive patients with giant MCA aneurysm associated to intracranial hypertension with a midline brain shift. Deep hypothermic circulatory flow reduction (DHCFR) with vascular reconstruction was performed in 4 patients and cerebral revascularization with aneurysm trapping was the therapeutic strategy in 5 patients. Early (< 7 days) and long term clinical and radiological monitoring was done. Good functional outcome was considered as mRS score ≤ 2 at 3 months. The mean age at treatment was 44 yo (ranged from 17 to 70 yo). The mean maximal diameter of the aneurysm was 49 mm (ranged from 33 to 70 mm). The mean midline brain shift was 8.6 mm (ranged from 5 to 13 mm). Distal MCA territory hypoperfusion was noted in 6 patients. Diffuse postoperative cerebral edema occurred in the 9 patients with a mean delay of 59 h and conducted to a postoperative neurological deterioration in 7 of them. Postoperative death was noted in 3 patients. Among the 6 survivors, early postoperative decompressive hemicraniotomy was required in 4 patients. Good functional outcome was noted in 4 patients. Complete aneurysm occlusion was noted in each patient at last follow-up. We suggest to discuss a systematic decompressive hemicraniotomy at the end of the surgical procedure and/or a partial temporal lobe resection at its beginning to reduce the consequences of the edema reaction and to improve the postoperative outcome of this specific subgroup of patients. A better intraoperative assessment of the blood flow might also reduce the occurrence of the reperfusion syndrome.

A study on the pressure‐driven flow of magnetized non‐Newtonian Casson fluid between two corrugated curved walls of an arbitrary phase difference

AbstractPressure‐driven movement is a fundamental concept with numerous applications in various industries, scientific disciplines, and fields of engineering. Its proper execution is vital for promoting revolutionary innovations and providing solutions in numerous sectors. Therefore, this article scrutinizes the pressure‐driven flow of magnetized Casson fluid between two curved corrugated walls. The geometry of the channel is represented mathematically in an orthogonal curvilinear coordinate system. The corrugation grooves are described by sinusoidal functions with phase differences between the corrugated curved walls. The boundary perturbation method is used to find the analytical solution for the velocity field and volumetric flow rate, taking the corrugation amplitude as the perturbation parameter. The results show that the peak of the velocity increases with the radius of curvature and the width of the channel for a constant pressure gradient. The velocity exhibited a declining trend due to an increase in the Casson fluid parameter. For a sufficiently large corrugation wavenumber, the flow rate decreases, and the phase difference becomes irrelevant. However, the reduction in flow can be minimized by decreasing the channel radius of curvature. In general, a smooth curved channel will give the maximum flow rate for a large corrugation wavenumber. The model can be used to simulate blood flow in arteries with varying geometries and magnetic fields, aiding in the study of cardiovascular diseases and the design of medical devices like stents.

Unveiling the role of long non-coding RNA MALAT1: a comprehensive review on myocardial infarction.

Myocardial infarction (MI) stands at top global causes of death in developed countries, owing mostly to atherosclerotic plaque growth and endothelial injury-induced reduction in coronary blood flow. While early reperfusion techniques have improved outcomes, long-term treatment continues to be difficult. The function of lncRNAs extends to regulating gene expression in various conditions, both physiological and pathological, such as cardiovascular diseases. The objective of this research is to extensively evaluate the significance of the lncRNA called Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in the development and management of MI. According to research, MALAT1 is implicated in processes such as autophagy, apoptosis, cell proliferation, and inflammation in the cardiovascular system. This investigation examines recent research examining the effects of MALAT1 on heart function and its potential as a mean of diagnosis and treatment for post- MI complications and ischemic reperfusion injury.

Social deficits mirror delayed cerebrovascular dysfunction after traumatic brain injury

Traumatic brain injury (TBI) survivors face debilitating long-term psychosocial consequences, including social isolation and depression. TBI modifies neurovascular physiology and behavior but the chronic physiological implications of altered brain perfusion on social interactions are unknown. Adult C57/BL6 male mice received a moderate cortical TBI, and social behaviors were assessed at baseline, 3-, 7-, 14-, 30-, and 60-days post injury (dpi). Magnetic resonance imaging (MRI, 9.4T) using dynamic susceptibility contrast perfusion weighted MRI were acquired. At 60dpi mice underwent histological angioarchitectural mapping. Analysis utilized standardized protocols followed by cross-correlation metrics. Social behavior deficits at 60dpi emerged as reduced interactions with a familiar cage-mate (partner) that mirrored significant reductions in cerebral blood flow (CBF) at 60dpi. CBF perturbations were dynamic temporally and across brain regions including regions known to regulate social behavior such as hippocampus, hypothalamus, and rhinal cortex. Social isolation in TBI-mice emerged with a significant decline in preference to spend time with a cage mate. Cortical vascular density was also reduced corroborating the decline in brain perfusion and social interactions. Thus, the late emergence of social interaction deficits mirrored the reduced vascular density and CBF in regions known to be involved in social behaviors. Vascular morphology and function improved prior to the late decrements in social function and our correlations strongly implicate a linkage between vascular density, cerebral perfusion, and social interactions. Our study provides a clinically relevant timeline of alterations in social deficits alongside functional vascular recovery that can guide future therapeutics.

Pyramid-Shaped Superhydrophobic Surfaces for Underwater Drag Reduction.

Superhydrophobic surfaces hold immense potential in underwater drag reduction. However, as the Reynolds number increases, the drag reduction rate decreases, and it may even lead to a drag increase. The reason lies in the collapse of the air mattress. To address this issue, this paper develops a pyramid-shaped robust superhydrophobic surface with wedged microgrooves, which exhibits a high gas fraction when immersed underwater and good ability to achieve complete spreading and recovery of the air mattress through air replenishment in the case of collapse of the air mattress. Pressure drop tests in a water tunnel confirm that with continuous air injection, the drag reduction reaches 64.8% in laminar flow conditions, substantially greater than 38.4% in the case without air injection, and can achieve 50.8% drag reduction in turbulent flow. This result highlights the potential applications of superhydrophobic surfaces with air mattress recovery for drag reduction.

Experimental Study on the Effects of Tapioca Starch on Cement Mortar Quality Improvement.

In this study, the effect of tapioca starch (TP) on mortar was evaluated by incorporating TP into the mortar mixture. The evaluation involved analyzing the mortar's quality characteristics, performance, and fundamental quality improvements. The addition of TP resulted in a decrease in flow, which was attributed to increased viscosity. Specifically, a 10% reduction in flow was observed with a 0.025% increase in TP content. After 28 days, the impact of TP on the compressive strength of the mortar remained consistent, regardless of the TP amount. However, within the first 3 days, higher TP content accelerated strength development, with early compressive strength increasing by up to 20% at a 0.050% TP level. Additionally, bond strength improved by approximately 60% at a 0.050% TP concentration, and final shrinkage was reduced by 5%.

Soil drought sets site specific limits to stem radial growth and sap flow of Douglas-fir across Germany.

Soil drought during summer in Central Europe has become more frequent and severe over the last decades. European forests are suffering increasing damage, particularly Norway spruce. Douglas-fir (Pseudotsuga menziesii (Mirbel) Franco), a non-native tree species, is considered as a promising alternative to build drought-resilient forests. The main goal of this study was to investigate the intraannual radial stem growth and sap flow performance of Douglas-fir along a precipitation gradient across Germany under severe drought. Sap flow and stem radial changes of up to ten trees each at four sites with different precipitation regimes were measured in combination with volumetric soil water content during the growing season of 2022. Measurements of stem radial changes were used to calculate the trees' stem water deficit, a proxy for tree water status and drought stress. The severe summer drought of 2022 led to an early growth cessation and a significant reduction in daily sap flow at all four sites monitored. We could identify a site-specific threshold in soil water availability ranging between 21.7 and 29.6% of relative extractable water (REW) under which stem water reserves cannot be replenished and thereby inhibiting radial growth. We could also demonstrate that at this threshold, sap flow is heavily reduced to between 43.5 and 53.3%, and for a REW below 50%, sap flow linearly decreases by 1.1-2.0% per 1% reduction in REW. This reduction tends to follow the humidity gradient, being more pronounced at the most oceanic characterized site and suggesting an adaptation to site conditions. Even though Douglas-fir is considered to be more drought stress resistant than Norway spruce, growth and sap flow are greatly reduced by severe summer drought, which became more frequent in recent years and their frequency and intensity is likely to increase. Our results suggest that timber production of Douglas-fir in Central Europe will decline considerably under projected climate change, and thus pointing to site specific growth constraints for a so far promising non-native tree species in Europe.

Lycium barbarum glycopeptide promotes neuroprotection in ET-1 mediated retinal ganglion cell degeneration

BackgroundVascular dysregulation is one of the major risk factors of glaucoma, and endothelin-1 (ET-1) may have a role in the pathogenesis of vascular-related glaucoma. Fruit extract from Lycium Barbarum (LB) exhibits anti-ageing and multitarget mechanisms in protecting retinal ganglion cells (RGC) in various animal models. To investigate the therapeutic efficacy of LB glycoproteins (LbGP) in ET-1 induced RGC degeneration, LbGP was applied under pre- and posttreatment conditions to an ET-1 mouse model. Retina structural and functional outcomes were characterised using clinical-based techniques.MethodsAdult C57BL/6 mice were randomly allocated into four experimental groups, namely vehicle control (n = 9), LbGP-Pretreatment (n = 8), LbGP-Posttreatment (day 1) (n = 8) and LbGP-Posttreatment (day 5) (n = 7). Oral administration of LbGP 1 mg/Kg or PBS for vehicle control was given once daily. Pre- and posttreatment (day 1 or 5) were commenced at 1 week before and 1 or 5 days after intravitreal injections, respectively, and were continued until postinjection day 28. Effects of treatment on retinal structure and functions were evaluated using optical coherence tomography (OCT), doppler OCT and electroretinogram measurements at baseline, post-injection days 10 and 28. RGC survival was evaluated by using RBPMS immunostaining on retinal wholemounts.ResultsET-1 injection in vehicle control induced transient reductions in arterial flow and retinal functions, leading to significant RNFL thinning and RGC loss at day 28. Although ET-1 induced a transient loss in blood flow or retinal functions in all LbGP groups, LbGP treatments facilitated better restoration of retinal flow and retinal functions as compared with the vehicle control. Also, all three LbGP treatment groups (i.e. pre- and posttreatments from days 1 or 5) significantly preserved thRNFL thickness and RGC densities. No significant difference in protective effects was observed among the three LbGP treatment groups.ConclusionLbGP demonstrated neuroprotective effects in a mouse model of ET-1 induced RGC degeneration, with treatment applied either as a pretreatment, immediate or delayed posttreatment. LbGP treatment promoted a better restoration of retinal blood flow, and protected the RNFL, RGC density and retinal functions. This study showed the translational potential of LB as complementary treatment for glaucoma management.

Cerebral hypoperfusion exacerbates vascular dysfunction after traumatic brain injury

Traumatic brain injuries are extremely common, and although most patients recover from their injuries many TBI patients suffer prolonged symptoms and remain at a higher risk for developing cardiovascular disease and neurodegeneration. Moreover, it remains challenging to identify predictors of poor long-term outcomes. Here, we tested the hypothesis that preexisting cerebrovascular impairment exacerbates metabolic and vascular dysfunction and leads to worse outcomes after TBI. Male mice underwent a mild surgical reduction in cerebral blood flow using a model of bilateral carotid artery stenosis (BCAS) wherein steel microcoils were implanted around the carotid arteries. Then, 30 days post coil implantation, mice underwent TBI or sham surgery. Gene expression profiles, cerebral blood flow, metabolic function, oxidative damage, vascular health and angiogenesis were assessed. Single nuclei RNA sequencing of endothelial cells isolated from mice after TBI showed differential gene expression profiles after TBI and BCAS, that were further altered when mice underwent both challenges. TBI but not BCAS increased mitochondrial oxidative metabolism. Both BCAS and TBI decreased cerebrovascular responses to repeated whisker stimulation. BCAS induced oxidative damage and inflammation in the vasculature as well as loss of vascular density, and reduced the numbers of angiogenic tip cells. Finally, intravascular protein accumulation was increased among mice that experienced both BCAS and TBI. Overall, our findings reveal that a prior vascular impairment significantly alters the profile of vascular health and function of the cerebrovasculature, and when combined with TBI may result in worsened outcomes.