For the purpose of improving product yield and reducing energy consumption, a novel cyclonic integrated catalytic process was established for the biomass vapors upgrading based on simultaneous reaction-separation technology. In order to gain better insights into the biomass pyrolysis vapors upgrading in this heterogeneous system of novel gradient cyclonic flow field, the particle transport hydrodynamics, the product distribution characteristics and operating parameters effects in the newly developed uniflow cyclone reactor were explored by experimental and numerical simulation methods. The results demonstrate that the gradient cyclonic flow field has a positive effect on the contact efficiency and separation effect, and then beneficially promote catalytic reactions. During the movement of catalysts in the gradient cyclonic flow field, a catalyst-cluster is observed near the top of the reactor with a dense “body” and a dilute “shadow”. The characteristics of secondary vortexes and solid concentration distribution on radial and axial directions were further explained. The optimal reaction temperature, reaction time and catalyst to vapors mass ratio to produce bio-oil respectively are 550 °C, 1.5 s and 24.0. This work provides theoretical basis for optimization of reactor structure and operation parameters. It is beneficial to the improvement of biomass catalytic pyrolysis technology.