Introduction. Currently, in connection with the large-scale introduction of the electronic educational resource in the educational process, it is especially relevant to search for optimal characteristics of presenting information on screens, taking into account the specifics of the electronic devices and age-related physiological features of the users’ visual system. The aim of the study was a physiological hygienic assessment of the impact of the font design of e-texts, presented on a laptop, on the psychophysiological state of students in grades 10-11. Material and methods. Psychophysiological testing of 43 schoolchildren in grades 10-11 without pathology of the vision organ was carried out on the NS-Psychotest complex, including the method of critical flicker fusion frequency to assess the state of the central part of the visual analyzer and the response to a moving object to determine the degree of the balance in nervous processes. The influence of reading illogical texts typed by a school headset was studied with a font size of 14, 12 and 10 points and a one-time reading of 200, 400 and 600 characters. Laptop Lenovo IdeaPad 720S-15 was used for the presentation of information. Results. High-grade schoolchildren were established to have the initial fatigue both of the visual analyzer and the nervous system as a whole. Boys showed better indices of the critical flicker fusion frequency than girls. Most schoolchildren had a balanced type of the inhibition and excitement processes. Methods of presenting information in electronic textbooks were shown to affect the psychophysiological state of high schoolchildren and may carry risks to their health. The hygienically rational design of electronic educational texts promotes the optimization of the psycho-functional state of the high schoolchildren’s body. Conclusion. The obtained results allow substantiating hygienic requirements for the font design of e-learning publications at the third stage of education.