Seepage control in unlined channels is critical for efficient water resource management, as excessive water loss through channel beds and walls leads to inefficiencies. Microbial-induced calcite precipitation (MICP) offers a promising solution to enhance soil stability and reduce seepage in cohesionless soils. This study investigates the application of MICP using Bacillus subtilis to mitigate water leakage in sandy soils through controlled experiments. A bacterial solution was introduced into soil samples, followed by urea and calcium chloride solutions, facilitating calcite precipitation and bioclogging. pH monitoring and calcium carbonate quantification confirmed the process. Results showed a 26% increase in calcium carbonate content in treated soil, significantly reducing permeability from 6.8 × 10−3 to 1.11 × 10−3 cm/s, as measured by a flexible wall permeameter. Soil strength increased from 234 to 1407 kPa, determined by triaxial compression tests. Validation was achieved using XRD, EDS, and SEM analyses. An interdisciplinary co-occurrence network graph from Scopus data highlighted key concepts like calcium carbonate, calcite precipitation, B. subtilis, bioclogging, and soil stabilization. MICP aligns with several UN Sustainable Development Goals (SDGs), notably SDG 6 by enhancing water retention, SDG 9 by promoting sustainable construction, and SDG 13 by improving soil stability against climate-related hazards. Thus, MICP is a sustainable and effective method for constructing impermeable channels, offering significant water management benefits.
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