Both of test and numerical simulation methods are employed to study the vertical progressive collapse and collapse assessment methods of steel frame under accidental loads. Firstly, a steel frame collapse test model with a geometric similarity ratio of 1:1.5 is designed and manufactured. The ends of column of test model is fixed. The upper and lower flange angle steel connection is used. The far end of the beam is constrained by a controllable sliding hinge support. The various stages of vertical progressive collapse of structures are simulated by a large displacement loads applied at the far end of the beam. The main focus of the testing is to examine the stress and deformation of beam and joint at various stages, particularly the development of stress and deformation in the angle steel attached to the beam-column connection. The development law of collapse load-displacement curve, joint rotation, beam strain, and angle steel strain are analyzed. The structural resistance mechanism and the transformation relationship between resistance mechanisms during collapse are studied. Subsequently, the relationship between the structural dynamic increase factor curve and the structural collapse process is analyzed using finite element method. The results show that the strain of angle steels can better reflect the destruction and deformation process of substructure during the collapse, the collapse resistance mechanism of structure, and the transition between resistance mechanisms.With the help of the characteristic points on the structural dynamic increase factor curve, the collapse limit state of structure can be reliably determined.